公开(公告)号：US07282126B2

公开(公告)日：2007-10-16

申请号：US10617750

申请日：2003-07-14

Applicant: Zhaowei Liu ,  Zhiyong Guo ,  Christina Maye ,  Kevin R. Gutshall

Inventor： Zhaowei Liu ,  Zhiyong Guo ,  Christina Maye ,  Kevin R. Gutshall

IPC: G01N27/453

CPC classification number: G01N27/44773 ,  C12Q1/6816 ,  C12Q1/6827 ,  C12Q2600/156 ,  G01N27/44704 ,  G01N27/44721 ,  C12Q2565/125 ,  C12Q2545/113 ,  C12Q2527/101 ,  C12Q2565/131 ,  C12Q2527/107

Abstract: The present invention relates to a method of determining the genotype of a sample polynucleotide having at least a first variant site. At least a portion of the sample polynucleotide is amplified to obtain first amplicons, the first amplicons including the first variant site. The first amplicons are combined with first and second different polynucleotide controls, the first and second polynucleotide controls differing by at least one base therealong, the position of the at least one differing base corresponding to the first variant site of the sample polynucleotide. A plurality of first duplexes are prepared, each of at least some of the first duplexes comprising (i) a polynucleotide strand of one of the first amplicons and (ii) a complementary polynucleotide strand of the first polynucleotide control. A plurality of second duplexes are prepared, each of at least some of the second duplexes comprising (i) a polynucleotide strand of one of the first amplicons and (ii) a complementary polynucleotide strand of the second polynucleotide control. The first and second duplexes are subjected to temperature gradient electrophoresis (TGE) to obtain first and second electrophoresis data. The genotype of the first variant site of the sample polynucleotide is determiend based on the first and second electrophoresis data.

Abstract translation: 本发明涉及确定具有至少第一变异位点的样品多核苷酸的基因型的方法。 扩增样品多核苷酸的至少一部分以获得第一扩增子，第一扩增子包括第一变体位点。 将第一扩增子与第一和第二不同多核苷酸对照组合，第一和第二多核苷酸对照与其中至少一个碱基不同，所述至少一个不同碱基对应于样品多核苷酸的第一个变异位点的位置。 制备多个第一双链体，至少一些第一双链体包含（i）第一扩增子之一的多核苷酸链和（ii）第一多核苷酸对照的互补多核苷酸链。 制备多个第二双链体，至少一些第二双链体包含（i）第一扩增子之一的多核苷酸链和（ii）第二多核苷酸对照的互补多核苷酸链。 对第一和​​第二双链体进行温度梯度电泳（TGE）以获得第一和第二电泳数据。 基于第一和第二电泳数据确定样品多核苷酸的第一变体位点的基因型。

公开(公告)号：US20050064473A1

公开(公告)日：2005-03-24

申请号：US10910256

申请日：2004-08-02

Applicant: Zhaowei Liu ,  Yiqiong Wu

Inventor： Zhaowei Liu ,  Yiqiong Wu

IPC: C12Q1/68

CPC classification number: C12Q1/6827 ,  C12Q1/6858 ,  C12Q2565/131

Abstract: A method for determining a frequency of single nucleotide polymorphism (SNP) within genomic DNA includes providing genomic DNA of each of a plurality of different organisms. The genomic DNA of each organism includes first and second portions, e.g., first and second strands. First and second amplicons are prepared from the genomic DNA of each organism. The first amplicon corresponds to the first portion of the genomic DNA and the second amplicon corresponds to the second portion of the genomic DNA. A plurality of duplexes is prepared from the first and second amplicons of the genomic DNA of each organism. At least some of the duplexes include a portion of one of the first amplicons and a portion of one of the second amplicons. The duplexes are subjected to temperature gradient electrophoresis to obtain first electrophoresis data indicative of the rate of SNP at a first location in the genomic DNA of the plurality of organisms.

Abstract translation: 用于确定基因组DNA内的单核苷酸多态性（SNP）的频率的方法包括提供多种不同生物体中的每一种的基因组DNA。 每个生物体的基因组DNA包括第一和第二部分，例如第一和第二链。 从每个生物体的基因组DNA制备第一和第二扩增子。 第一扩增子对应于基因组DNA的第一部分，第二扩增子对应于基因组DNA的第二部分。 从每个生物体的基因组DNA的第一和第二扩增子制备多个双链体。 至少一些双链体包括第一扩增子之一和第二扩增子之一的一部分的一部分。 对双链体进行温度梯度电泳以获得指示多个生物体的基因组DNA中第一位置处的SNP的速率的第一电泳数据。

公开(公告)号：US20050064400A1

公开(公告)日：2005-03-24

申请号：US10287808

申请日：2002-11-05

Applicant: Zhiyong Guo ,  Zhaowei Liu ,  Qingbo Li

Inventor： Zhiyong Guo ,  Zhaowei Liu ,  Qingbo Li

IPC: C12Q1/68 ,  C12Q1/6827 ,  G01N33/48 ,  G01N33/50 ,  G06F19/00

CPC classification number: C12Q1/6827 ,  C12Q2565/125 ,  C12Q2527/101

Abstract: The present invention relates to a method for determining the presence of a mutation in a first sample comprising first polynucleotides. The reference sample comprises reference polynucleotides. The first sample and a reference sample are subjected to electrophoresis in the presence of at least one intercalating dye. During electrophoresis the temperature of the first sample and the reference sample is changed by an amount sufficient to change an electrophoretic mobility of at least one of the first or reference polynucleotides. Fluorescence intensity data are obtained. The fluorescence intensity data are indicative of the presence of the first and reference polynucleotides. The data are processed to determine the presence of mutation in the first polynucleotides.

Abstract translation: 本发明涉及一种用于确定包含第一多核苷酸的第一样品中突变存在的方法。 参考样品包括参考多核苷酸。 第一个样品和参考样品在至少一个插层染料存在下进行电泳。 在电泳期间，第一样品和参考样品的温度改变足以改变至少一个第一或参考多核苷酸的电泳迁移率的量。 获得荧光强度数据。 荧光强度数据表明第一和参考多核苷酸的存在。 处理数据以确定第一多核苷酸中突变的存在。

公开(公告)号：US09304234B2

公开(公告)日：2016-04-05

申请号：US13418097

申请日：2012-03-12

Applicant: Zhaowei Liu ,  Feifei Wei

Inventor： Zhaowei Liu ,  Feifei Wei

IPC: G02B5/00 ,  G01N21/552 ,  G01N21/64

CPC classification number: G02B5/008 ,  G01N21/553 ,  G01N21/6458 ,  G01N21/648 ,  G01N2201/062 ,  G01N2201/0628

Abstract: Plasmonic condensers for generating surface plasmon at an evanescent wave surface can include a substrate layer, a metal layer comprising the evanescent wave surface; and a media layer disposed between the metal layer and the substrate layer. The media layer can be active or passive and can include a source of radiation that interacts with the metal layer to create surface plasmons that are not substantially optically detectable as far field radiation until an interfering object is brought into proximity with the evanescent wave surface. When an interfering object such as a sample or specimen is brought into proximity with the evanescent wave surface, it causes coupling of at least some of the surface plasmons into propagating radiation detectable by an objective lens. Systems, methods, and the like are disclosed, as are features of a plasmonic meta-materials illuminator.

Abstract translation: 用于在ev逝波表面产生表面等离子体激元的等离子体冷凝器可以包括基底层，包含ev逝波面的金属层; 以及设置在金属层和基底层之间的介质层。 介质层可以是有源的或无源的，并且可以包括与金属层相互作用的辐射源，以产生表面等离子体，这些表面等离激元不是远场光辐射，直到干扰物体接近于ev逝波面。 当诸如样品或样品的干扰物体与ev逝波表面接近时，其使至少一些表面等离子体激元耦合到由物镜可检测的传播辐射。 公开了系统，方法等，以及等离子体激元间材料照明器的特征。

公开(公告)号：US07303879B2

公开(公告)日：2007-12-04

申请号：US10910256

申请日：2004-08-02

Applicant: Zhaowei Liu ,  Yiqiong Wu

Inventor： Zhaowei Liu ,  Yiqiong Wu

IPC: C12Q1/68 ,  C07H21/02 ,  C07H21/04

CPC classification number: C12Q1/6827 ,  C12Q1/6858 ,  C12Q2565/131

Abstract: A method for determining a frequency of single nucleotide polymorphism (SNP) within genomic DNA includes providing genomic DNA of each of a plurality of different organisms. The genomic DNA of each organism includes first and second portions, e.g., first and second strands. First and second amplicons are prepared from the genomic DNA of each organism. The first amplicon corresponds to the first portion of the genomic DNA and the second amplicon corresponds to the second portion of the genomic DNA.A plurality of duplexes is prepared from the first and second amplicons of the genomic DNA of each organism. At least some of the duplexes include a portion of one of the first amplicons and a portion of one of the second amplicons.The duplexes are subjected to temperature gradient electrophoresis to obtain first electrophoresis data indicative of the rate of SNP at a first location in the genomic DNA of the plurality of organisms.

Abstract translation: 用于确定基因组DNA内的单核苷酸多态性（SNP）的频率的方法包括提供多种不同生物体中的每一种的基因组DNA。 每个生物体的基因组DNA包括第一和第二部分，例如第一和第二链。 从每个生物体的基因组DNA制备第一和第二扩增子。 第一扩增子对应于基因组DNA的第一部分，第二扩增子对应于基因组DNA的第二部分。