公开(公告)号：US20160348093A1

公开(公告)日：2016-12-01

申请号：US15157643

申请日：2016-05-18

Applicant: 10X Genomics, Inc.

Inventor： Andrew D. Price ,  Christopher Hindson ,  Rajiv Bharadwaj ,  Sukhvinder Kaur ,  Geoffrey McDermott

IPC: C12N15/10 ,  C12Q1/68

CPC classification number: C12N15/1006 ,  C12Q1/6806 ,  C12Q1/6834 ,  C12Q2527/119 ,  C12Q2527/125 ,  C12Q2527/153 ,  C12Q2563/149 ,  C12Q2563/179 ,  C12Q2565/629 ,  C12Q2563/159

Abstract: Compositions that include particle suspensions where such particle suspensions have characteristics for use in a variety of applications including, for example, flow restriction, reagent delivery, and use in microfluidic systems. In some compositions provided, the particle suspension include deformable particles and in particular compositions the deformable particles are beads or gel beads.

Abstract translation: 包括颗粒悬浮液的组合物，其中这种颗粒悬浮液具有用于各种应用的特征，包括例如流动限制，试剂递送和在微流体系统中的应用。 在提供的一些组合物中，颗粒悬浮液包括可变形颗粒，特别是组合物中，可变形颗粒是珠粒或凝胶珠。

公开(公告)号：US12264316B2

公开(公告)日：2025-04-01

申请号：US16419428

申请日：2019-05-22

Applicant: 10X Genomics, Inc.

Inventor： Zahra Kamila Belhocine ,  Rajiv Bharadwaj ,  Christopher Hindson ,  Michael Schnall-Levin ,  Bill Kengli Lin ,  Anthony Makarewicz ,  Pranav Patel ,  Katherine Pfeiffer ,  Andrew D. Price ,  Mohammad Rahimi Lenji ,  Tobias Daniel Wheeler ,  Yifeng Yin

IPC: C12N15/10 ,  C12Q1/6806 ,  C12Q1/6869

Abstract: Methods and systems are provided for sample preparation techniques and sequencing of macromolecular constituents of cells and other biological materials.

公开(公告)号：US12117378B2

公开(公告)日：2024-10-15

申请号：US18205397

申请日：2023-06-02

Applicant: 10X GENOMICS, INC.

Inventor： Christopher Hindson ,  Andrew D. Price ,  Michael Schnall-Levin

IPC: G01N1/28 ,  C12N5/00 ,  G01N1/40

CPC classification number: G01N1/28 ,  C12N5/0006 ,  G01N1/4077 ,  C12N2537/10

Abstract: Methods and systems for cell and bead processing or analysis are provided. A cell or bead can be subjected to conditions sufficient to change a first characteristic or set of characteristics (e.g., cell or bead size). The cell or bead can further be subjected to conditions sufficient to change a second characteristic or set of characteristics. In some cases, crosslinks are formed within the cell or bead.

公开(公告)号：US12084716B2

公开(公告)日：2024-09-10

申请号：US17473760

申请日：2021-09-13

Applicant: 10X Genomics, Inc.

Inventor： Phillip Belgrader ,  Zachary Bent ,  Rajiv Bharadwaj ,  Vijay Kumar Sreenivasa Gopalan ,  Josephine Harada ,  Christopher Hindson ,  Mohammad Rahimi Lenji ,  Michael Ybarra Lucero ,  Geoffrey McDermott ,  Elliott Meer ,  Tarjei Sigurd Mikkelsen ,  Christopher Joachim O'Keeffe ,  Katherine Pfeiffer ,  Andrew D. Price ,  Paul Ryvkin ,  Serge Saxonov ,  John R. Stuelpnagel ,  Jessica Michele Terry ,  Tobias Daniel Wheeler ,  Indira Wu ,  Solongo Batjargal Ziraldo ,  Stephane Claude Boutet ,  Sarah Taylor ,  Niranjan Srinivas

IPC: C12Q1/68 ,  C12Q1/6806 ,  C12Q1/683 ,  C12Q1/6874

CPC classification number: C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/683 ,  C12Q2525/191 ,  C12Q2537/143 ,  C12Q2563/179

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization. Such polynucleotide processing may be useful for a variety of applications, including analyte characterization by polynucleotide sequencing. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins), genomic DNA, and RNA (e.g., mRNA or CRISPR guide RNAs). Also described herein, are barcoded labelling agents and oligonucleotide molecules useful for “tagging” analytes for characterization.

公开(公告)号：US11636921B2

公开(公告)日：2023-04-25

申请号：US16698612

申请日：2019-11-27

Applicant: 10X Genomics, Inc.

Inventor： Michael Schnall-Levin ,  Rajiv Bharadwaj ,  Zahra Kamila Belhocine ,  Andrew D. Price ,  Yifeng Yin ,  Vijay Kumar Sreenivasa Gopalan ,  Zeljko Jovan Dzakula

IPC: G16B30/00 ,  G16H10/40 ,  G16H20/00

Abstract: Systems and methods for inferring a status of a cell population are provided. Described techniques allow deconvolving a first clonal population comprising a first plurality of cells of a species, wherein nucleic acid sequence reads from each cell in the first plurality of cells are obtained. The nucleic acid sequence reads are mapped into bins representing portions of a reference genome, and a pattern of sequence read counts for each cell across the multiple bins is used to assign a cell to a group, thereby inferring a mitotic status of the cell. The assignment of nucleic acid sequence reads into bins is also be used for segregating cells into classes based on a status of a certain biological marker in each cell. Comparison of sequence read counts for a subset of bins across the cell classes allows evaluating effect of a compound on a cell status.

公开(公告)号：US11193122B2

公开(公告)日：2021-12-07

申请号：US17208369

申请日：2021-03-22

Applicant: 10X Genomics, Inc.

Inventor： Zahra Kamila Belhocine ,  Rajiv Bharadwaj ,  Christopher Hindson ,  Michael Schnall-Levin ,  Bill Kengli Lin ,  Anthony Makarewicz ,  Pranav Patel ,  Andrew D. Price ,  Mohammad Rahimi Lenji ,  Tobias Daniel Wheeler ,  Yifeng Yin

IPC: C12N15/10 ,  C12Q1/6806 ,  C12Q1/6869

Abstract: Methods and systems are provided for sample preparation techniques and sequencing of macromolecular constituents of cells and other biological materials.

公开(公告)号：US20210222158A1

公开(公告)日：2021-07-22

申请号：US17208369

申请日：2021-03-22

Applicant: 10X Genomics, Inc.

Inventor： Zahra Kamila Belhocine ,  Rajiv Bharadwaj ,  Christopher Hindson ,  Michael Schnall-Levin ,  Bill Kengli Lin ,  Anthony Makarewicz ,  Pranav Patel ,  Katherine Pfeiffer ,  Andrew D. Price ,  Mohammad Rahimi Lenji ,  Tobias Daniel Wheeler ,  Yifeng Yin

IPC: C12N15/10 ,  C12Q1/6806 ,  C12Q1/6869

Abstract: Methods and systems are provided for sample preparation techniques and sequencing of macromolecular constituents of cells and other biological materials.

公开(公告)号：US20210040535A1

公开(公告)日：2021-02-11

申请号：US16915493

申请日：2020-06-29

Applicant: 10X GENOMICS, INC.

Inventor： Adam Lowe ,  Christopher Hindson ,  Andrew D. Price

IPC: C12Q1/6806 ,  C12N15/10 ,  B01J19/00 ,  B01J13/04

Abstract: The present disclosure provides particles (e.g., beads) and methods, kits, and systems involving the same for sample processing or analysis. Such particles may include one or more analytes, one or more reagents, and two or more gel components and/or walled components. The particles described herein may be formed, for example, by polymerization of a polymerizable material in proximity to a gel or walled component.

公开(公告)号：US20200239874A1

公开(公告)日：2020-07-30

申请号：US16554564

申请日：2019-08-28

Applicant: 10X GENOMICS, INC.

Inventor： Tarjei Sigurd Mikkelsen ,  Eswar Prasad Ramachandran Iyer ,  Andrew Kohlway ,  Luigi Jhon Alvarado Martinez ,  Katherine Pfeiffer ,  Andrew D. Price

IPC: C12N15/10 ,  C12Q1/682 ,  C12Q1/6855

Abstract: The present disclosure provides methods of processing or analyzing a sample. A method for processing a sample may comprise hybridizing a probe molecule to a target region of a nucleic acid molecule (e.g., a ribonucleic acid (RNA) molecule), barcoding the probe-nucleic acid molecule complex, and performing extension, denaturation, and amplification processes. A method for processing a sample may comprise hybridizing first and second probes to adjacent or non-adjacent target regions of a nucleic acid molecule (e.g., an RNA molecule), linking the first and second probes to provide a probe-linked nucleic acid molecule, and barcoding the probe-linked nucleic acid molecule. One or more processes of the methods described herein may be performed within a partition, such as a droplet or well. One or more processes of the methods described herein may be performed on a cell, such as a permeabilized cell.

公开(公告)号：US10590244B2

公开(公告)日：2020-03-17

申请号：US16178430

申请日：2018-11-01

Applicant: 10X Genomics, Inc.

Inventor： Joshua Delaney ,  Shalini Gohil ,  Christopher Hindson ,  Adam Lowe ,  Andrew D. Price

IPC: C08J3/075 ,  C08J3/24 ,  B01L3/00

Abstract: The present disclosure provides systems and methods for making a hydrogel comprising a cell, cell nucleus, or one or more components derived from a cell or cell nucleus. A method for making a hydrogel may comprise providing a cell or cell nucleus, a first polymer, wherein the first polymer comprises a plurality of first crosslink precursors, each of the plurality of first crosslink precursors comprising an azide group; providing a second polymer, wherein the second polymer comprises a plurality of second crosslink precursors, each of the plurality of second crosslink precursors comprising an alkyne group; and crosslinking the first polymer and the second polymer via a reaction between a first section of the first crosslink precursors and a second section of the second crosslink precursors, thereby providing the hydrogel comprising the cell or cell nucleus.