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11.发明申请System having device for preventing air bubbles in a hybridization chamber and corresponding method 有权具有用于在杂交室中防止气泡的装置的系统和相应的方法公开(公告)号：US20060008812A1
公开(公告)日：2006-01-12
申请号：US10931432
申请日：2004-09-01
CPC分类号： B01L3/502723 , B01L3/50855 , B01L2200/0684 , B01L2300/0822 , B01L2300/14
摘要： The present invention relates to a system (1) having hybridization chambers (5) for hybridizing nucleic acid samples, proteins, or tissue sections immobilized on slides (27), each hybridization chamber (5) being defined as an essentially gap-shaped chamber, which is essentially fillable with a liquid, between one of these slides (27) and a cover (26), and the cover (26) being positioned in relation to the slide (27) in such a way that the hybridization chamber (5) is sealed to the surrounding air, the system (1) including a device for preventing air bubbles in the hybridization chambers (5). The system according to the present invention is distinguished in that this device for preventing air bubbles in the hybridization chambers (5) is implemented as a pressure device to build up a chamber pressure in the hybridization chambers (5), this chamber pressure lying above the normal atmospheric pressure existing in the surrounding air. The present invention additionally relates to a method for preventing air bubbles in the hybridization chambers (5) of such a system (1) and is distinguished in that, using a pressure device of this system (1), a chamber pressure is implemented in the hybridization chambers (5) which lies above the normal atmospheric pressure existing in the surrounding air.
摘要翻译： 本发明涉及一种具有杂交室（5）的系统（1），用于将固定在载片（27）上的核酸样品，蛋白质或组织切片进行杂交，每个杂交室（5）被定义为基本间隙形的室， 其基本上可用液体填充，在这些滑动件（27）中的一个和盖（26）之间，并且盖（26）相对于载玻片（27）定位成使得杂交室（5） 密封到周围空气，系统（1）包括用于防止杂交室（5）中的气泡的装置。 根据本发明的系统的特征在于，用于防止杂交室（5）中的气泡的装置被实施为用于在杂交室（5）中建立室压力的压力装置，该室压力位于 周围空气中存在正常的大气压力。 本发明还涉及一种用于防止这种系统（1）的杂交室（5）中的气泡的方法，其区别在于，使用该系统（1）的压力装置，在 位于周围空气中存在的正常大气压力之上的杂交室（5）。
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12.发明授权System having device for preventing air bubbles in a hybridization chamber and corresponding method 有权具有用于在杂交室中防止气泡的装置的系统和相应的方法公开(公告)号：US07615370B2
公开(公告)日：2009-11-10
申请号：US10931432
申请日：2004-09-01
CPC分类号： B01L3/502723 , B01L3/50855 , B01L2200/0684 , B01L2300/0822 , B01L2300/14
摘要： The present invention relates to a system (1) having hybridization chambers (5) for hybridizing nucleic acid samples, proteins, or tissue sections immobilized on slides (27), each hybridization chamber (5) being defined as an essentially gap-shaped chamber, which is essentially fillable with a liquid, between one of these slides (27) and a cover (26), and the cover (26) being positioned in relation to the slide (27) in such a way that the hybridization chamber (5) is sealed to the surrounding air, the system (1) including a device for preventing air bubbles in the hybridization chambers (5). The system according to the present invention is distinguished in that this device for preventing air bubbles in the hybridization chambers (5) is implemented as a pressure device to build up a chamber pressure in the hybridization chambers (5), this chamber pressure lying above the normal atmospheric pressure existing in the surrounding air. The present invention additionally relates to a method for preventing air bubbles in the hybridization chambers (5) of such a system (1) and is distinguished in that, using a pressure device of this system (1), a chamber pressure is implemented in the hybridization chambers (5) which lies above the normal atmospheric pressure existing in the surrounding air.
摘要翻译： 本发明涉及一种具有杂交室（5）的系统（1），用于将固定在载片（27）上的核酸样品，蛋白质或组织切片进行杂交，每个杂交室（5）被定义为基本间隙形的室， 其基本上可用液体填充，在这些滑动件（27）中的一个和盖（26）之间，并且盖（26）相对于载玻片（27）定位成使得杂交室（5） 密封到周围空气，系统（1）包括用于防止杂交室（5）中的气泡的装置。 根据本发明的系统的特征在于，用于防止杂交室（5）中的气泡的装置被实施为用于在杂交室（5）中建立室压力的压力装置，该室压力位于 周围空气中存在正常的大气压力。 本发明还涉及一种用于防止这种系统（1）的杂交室（5）中的气泡的方法，其区别在于，使用该系统（1）的压力装置，在 位于周围空气中存在的正常大气压力之上的杂交室（5）。
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公开(公告)号：US20060003440A1
公开(公告)日：2006-01-05
申请号：US11191327
申请日：2005-07-28
IPC分类号： C12M1/34
CPC分类号： B01L3/502715 , B01F5/10 , B01F11/0071 , B01F13/0059 , B01F15/00123 , B01F15/00318 , B01F15/00396 , B01F15/0244 , B01F2015/00084 , B01F2215/0037 , B01F2215/0073 , B01J2219/00353 , B01J2219/00396 , B01J2219/00477 , B01J2219/00479 , B01J2219/00533 , B01J2219/00659 , B01L3/50273 , B01L3/502738 , B01L3/50855 , B01L9/06 , B01L2200/0689 , B01L2300/0636 , B01L2300/0822 , B01L2300/0877 , B01L2400/0481 , C40B60/14
摘要： A device provides a hybridization chamber for hybridizing nucleic acid samples, proteins or tissue sections on a slide, and is an essentially rectangular body movable opposite the slide. The device includes a surface, lines for supplying and/or removing media, a specimen supply line and an agitating device. The agitation device includes at least one membrane which separates a pressure chamer to be fillable with a pressure fluid via one of the lines, from at least one agitation chamber connected via an agitation line to the hybridization chamber. The lines are continuously open so the media therein can permanently flow unhindered. Four such devices can be combined into a process unit for providing a hybridization chamber. Such unit is pivotable around an axis and has a holder with four seats, which is lockable in relation to a baseplate, one device being insertable into each one of the seats.
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