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    System having device for preventing air bubbles in a hybridization chamber and corresponding method
    1.
    发明申请
    System having device for preventing air bubbles in a hybridization chamber and corresponding method 有权
    具有用于在杂交室中防止气泡的装置的系统和相应的方法

    公开(公告)号：US20060008812A1

    公开(公告)日：2006-01-12

    申请号：US10931432

    申请日：2004-09-01

    申请人： Wolfgang Streit ,  Gyoergy Wenczel ,  Waltraud Lamprecht ,  Heribert Eglauer

    发明人： Wolfgang Streit ,  Gyoergy Wenczel ,  Waltraud Lamprecht ,  Heribert Eglauer

    IPC分类号： C12Q1/68 ,  C12M1/34

    CPC分类号： B01L3/502723 ,  B01L3/50855 ,  B01L2200/0684 ,  B01L2300/0822 ,  B01L2300/14

    摘要： The present invention relates to a system (1) having hybridization chambers (5) for hybridizing nucleic acid samples, proteins, or tissue sections immobilized on slides (27), each hybridization chamber (5) being defined as an essentially gap-shaped chamber, which is essentially fillable with a liquid, between one of these slides (27) and a cover (26), and the cover (26) being positioned in relation to the slide (27) in such a way that the hybridization chamber (5) is sealed to the surrounding air, the system (1) including a device for preventing air bubbles in the hybridization chambers (5). The system according to the present invention is distinguished in that this device for preventing air bubbles in the hybridization chambers (5) is implemented as a pressure device to build up a chamber pressure in the hybridization chambers (5), this chamber pressure lying above the normal atmospheric pressure existing in the surrounding air. The present invention additionally relates to a method for preventing air bubbles in the hybridization chambers (5) of such a system (1) and is distinguished in that, using a pressure device of this system (1), a chamber pressure is implemented in the hybridization chambers (5) which lies above the normal atmospheric pressure existing in the surrounding air.

    摘要翻译： 本发明涉及一种具有杂交室（5）的系统（1），用于将固定在载片（27）上的核酸样品，蛋白质或组织切片进行杂交，每个杂交室（5）被定义为基本间隙形的室， 其基本上可用液体填充，在这些滑动件（27）中的一个和盖（26）之间，并且盖（26）相对于载玻片（27）定位成使得杂交室（5） 密封到周围空气，系统（1）包括用于防止杂交室（5）中的气泡的装置。 根据本发明的系统的特征在于，用于防止杂交室（5）中的气泡的装置被实施为用于在杂交室（5）中建立室压力的压力装置，该室压力位于 周围空气中存在正常的大气压力。 本发明还涉及一种用于防止这种系统（1）的杂交室（5）中的气泡的方法，其区别在于，使用该系统（1）的压力装置，在 位于周围空气中存在的正常大气压力之上的杂交室（5）。

    System having device for preventing air bubbles in a hybridization chamber and corresponding method
    2.
    发明授权
    System having device for preventing air bubbles in a hybridization chamber and corresponding method 有权
    具有用于在杂交室中防止气泡的装置的系统和相应的方法

    公开(公告)号：US07615370B2

    公开(公告)日：2009-11-10

    申请号：US10931432

    申请日：2004-09-01

    申请人： Wolfgang Streit ,  Gyoergy Wenczel ,  Waltraud Lamprecht ,  Heribert Eglauer

    发明人： Wolfgang Streit ,  Gyoergy Wenczel ,  Waltraud Lamprecht ,  Heribert Eglauer

    IPC分类号： C12M1/34 ,  C12M3/00

    CPC分类号： B01L3/502723 ,  B01L3/50855 ,  B01L2200/0684 ,  B01L2300/0822 ,  B01L2300/14

    摘要： The present invention relates to a system (1) having hybridization chambers (5) for hybridizing nucleic acid samples, proteins, or tissue sections immobilized on slides (27), each hybridization chamber (5) being defined as an essentially gap-shaped chamber, which is essentially fillable with a liquid, between one of these slides (27) and a cover (26), and the cover (26) being positioned in relation to the slide (27) in such a way that the hybridization chamber (5) is sealed to the surrounding air, the system (1) including a device for preventing air bubbles in the hybridization chambers (5). The system according to the present invention is distinguished in that this device for preventing air bubbles in the hybridization chambers (5) is implemented as a pressure device to build up a chamber pressure in the hybridization chambers (5), this chamber pressure lying above the normal atmospheric pressure existing in the surrounding air. The present invention additionally relates to a method for preventing air bubbles in the hybridization chambers (5) of such a system (1) and is distinguished in that, using a pressure device of this system (1), a chamber pressure is implemented in the hybridization chambers (5) which lies above the normal atmospheric pressure existing in the surrounding air.

    摘要翻译： 本发明涉及一种具有杂交室（5）的系统（1），用于将固定在载片（27）上的核酸样品，蛋白质或组织切片进行杂交，每个杂交室（5）被定义为基本间隙形的室， 其基本上可用液体填充，在这些滑动件（27）中的一个和盖（26）之间，并且盖（26）相对于载玻片（27）定位成使得杂交室（5） 密封到周围空气，系统（1）包括用于防止杂交室（5）中的气泡的装置。 根据本发明的系统的特征在于，用于防止杂交室（5）中的气泡的装置被实施为用于在杂交室（5）中建立室压力的压力装置，该室压力位于 周围空气中存在正常的大气压力。 本发明还涉及一种用于防止这种系统（1）的杂交室（5）中的气泡的方法，其区别在于，使用该系统（1）的压力装置，在 位于周围空气中存在的正常大气压力之上的杂交室（5）。

    Device and method for providing a hybridization chamber and for influencing air bubbles in the same
    3.
    发明授权
    Device and method for providing a hybridization chamber and for influencing air bubbles in the same 有权
    用于提供杂交室并用于影响其中气泡的装置和方法

    公开(公告)号：US07459306B2

    公开(公告)日：2008-12-02

    申请号：US10909521

    申请日：2004-08-02

    申请人： Wolfgang Streit ,  Gyoergy Wenczel ,  Waltraud Lamprecht ,  Heribert Eglauer

    发明人： Wolfgang Streit ,  Gyoergy Wenczel ,  Waltraud Lamprecht ,  Heribert Eglauer

    IPC分类号： C12M1/34 ,  C12M3/00 ,  B01L3/00

    CPC分类号： B01D19/0021 ,  B01F13/0059 ,  B01F2005/0621 ,  B01L3/502723 ,  B01L3/502746 ,  B01L7/52 ,  B01L2200/0684 ,  B01L2300/0636 ,  B01L2300/0822 ,  B01L2300/0877 ,  B01L2300/1805 ,  B01L2400/086

    摘要： A device, appropriate process units and/or systems for providing a gap-shaped hybridization space to hybridize nucleic acid samples, proteins or tissue sections over a slide. Said items of device are able to move in relation to the slide and comprise an annular sealing surface to seal the hybridization space by application to a surface of the slide. Said items of device additionally comprise lines to introduce media to and remove them from the hybridization space. Said items of device furthermore comprise a specimen supply line and preferably an agitation device to move fluids in relation to the samples immobilized on the surface of the slide. The devices according to the invention are characterized in that they limit a hybridization space that features relief structures to conduct and/or block air bubbles on at least one surface of the device and the slide directed at the interior of this hybridization space. According to the method of the invention, air bubbles present in the hybridization space are conducted and/or blocked by these relief structures.

    摘要翻译： 用于提供间隙形杂交空间以在载玻片上杂交核酸样品，蛋白质或组织切片的装置，适当的处理单元和/或系统。 所述装置的物品能够相对于滑块移动，并且包括环形密封表面，以通过施加到载玻片的表面来密封杂交空间。 所述装置的项目还包括将介质引入杂交空间并将其从杂交空间移除的线。 所述装置的物品还包括样品供应管线，优选地是相对于固定在载玻片表面上的样品移动流体的搅拌装置。 根据本发明的装置的特征在于，它们限制了杂交空间，该杂交空间具有浮雕结构以在装置的至少一个表面和指向该杂交空间内部的载玻片上传导和/或阻挡气泡。 根据本发明的方法，杂交空间中存在的气泡由这些浮雕结构进行和/或阻挡。

    Device and method for providing a hybridization chamber and for influencing air bubbles in the same
    4.
    发明申请
    Device and method for providing a hybridization chamber and for influencing air bubbles in the same 有权
    用于提供杂交室并用于影响其中气泡的装置和方法

    公开(公告)号：US20060014269A1

    公开(公告)日：2006-01-19

    申请号：US10909521

    申请日：2004-08-02

    申请人： Wolfgang Streit ,  Gyoergy Wenczel ,  Waltraud Lamprecht ,  Heribert Eglauer

    发明人： Wolfgang Streit ,  Gyoergy Wenczel ,  Waltraud Lamprecht ,  Heribert Eglauer

    IPC分类号： C12M1/34

    CPC分类号： B01D19/0021 ,  B01F13/0059 ,  B01F2005/0621 ,  B01L3/502723 ,  B01L3/502746 ,  B01L7/52 ,  B01L2200/0684 ,  B01L2300/0636 ,  B01L2300/0822 ,  B01L2300/0877 ,  B01L2300/1805 ,  B01L2400/086

    摘要： A device (1), appropriate process units (18) and/or systems (38) for providing a gap-shaped hybridization space (2) to hybridize nucleic acid samples, proteins or tissue sections over a slide (3). Said items of device (1) are able to move in relation to the slide (3) and comprise an annular sealing surface (4) to seal the hybridization space (2) by application to a surface (5) of the slide (3). Said items of device (1) additionally comprise lines (6,6′) to introduce media to and remove them from the hybridization space (2). Said items of device (1) furthermore comprise a specimen supply line (7) and preferably an agitation device (8) to move fluids in relation to the samples immobilized on the surface (5) of the slide (3). The devices according to the invention are characterized in that they limit a hybridization space (2) that features relief structures (101) to conduct and/or block air bubbles (102) on at least one surface (5,14) of the device (1) and the slide (3) directed at the interior of this hybridization space (2). According to the method of the invention, air bubbles (102) present in the hybridization space (2) are conducted and/or blocked by these relief structures (101).

    摘要翻译： 一种用于提供间隙形杂交空间（2）的装置（1），适当的处理单元（18）和/或系统（38），以使载体（3）上的核酸样品，蛋白质或组织切片杂交。 装置（1）的所述物品能够相对于滑动件（3）移动并且包括环形密封表面（4），以通过施加到滑动件（3）的表面（5）来密封杂交空间（2） 。 装置（1）的所述物品还包括用于将介质引入杂交空间（2）并将其从杂交空间（2）中移除的线（6,6'）。 装置（1）的所述物品还包括样品供应管线（7），优选地相对于固定在载玻片（3）的表面（5）上的样品）移动流体的搅拌装置（8）。 根据本发明的装置的特征在于它们限制了具有浮雕结构（101）以在装置的至少一个表面（5,14）上传导和/或阻挡气泡（102）的杂交空间（2） 1）和指向该杂交空间（2）内部的载玻片（3）。 根据本发明的方法，存在于杂交空间（2）中的气泡（102）由这些浮雕结构（101）进行和/或阻挡。

    Setting method for microplate washing devices
    8.
    发明授权
    Setting method for microplate washing devices 有权
    微孔板清洗装置的设定方法

    公开(公告)号：US09333505B2

    公开(公告)日：2016-05-10

    申请号：US13357080

    申请日：2012-01-24

    申请人： Wolfgang Streit ,  Juha Koota ,  Wolfgang Fuchs

    发明人： Wolfgang Streit ,  Juha Koota ,  Wolfgang Fuchs

    IPC分类号： B08B7/04 ,  B08B7/00 ,  B01L99/00

    CPC分类号： B01L99/00 ,  B01L2200/08 ,  B01L2200/14

    摘要： Setting method for microplate washing devices has a receptacle for receiving a microplate and a washing head having washing cannulas. The cannulas are in an array corresponding to a well array with lowermost ends define a work plane. This work plane is parallel to a reference plane. In a first phase, the receptacle and/or washing head are moved together until the lowermost ends of the cannulas touch a surface defining the reference plane. A sensor device has a controller linked thereto and from the sensor device is registered using the controller and a relative altitude value is determined therewith, which indicates touching of the surface by the lowermost ends of the cannulas or for determining the position of this surface. Based on this, an active altitude of the lowermost ends of the washing cannulas in relation to an inner surface of the well bottoms of a microplate is determined.

    摘要翻译： 微孔板洗涤装置的设置方法具有容纳微孔板的容器和具有清洗插管的清洗头。 插管是对应于具有最下端限定工作平面的井阵列的阵列。 该工作平面与参考平面平行。 在第一阶段，容器和/或洗涤头一起移动，直到插管的最下端接触限定参考平面的表面。 传感器装置具有连接到其上的控制器，并且使用控制器登记传感器装置，并且由此确定相对高度值，其指示由插管的最下端接触表面或用于确定该表面的位置。 基于此，确定相对于微板的井底的内表面的洗涤插管的最下端的主动高度。

    NOVEL GROUP OF ESTERASES FOR THE PRODUCTION OF FINE AND SPECIALITY CHEMICALS
    9.
    发明申请
    NOVEL GROUP OF ESTERASES FOR THE PRODUCTION OF FINE AND SPECIALITY CHEMICALS 有权

    公开(公告)号：US20090221031A1

    公开(公告)日：2009-09-03

    申请号：US12298874

    申请日：2007-04-27

    申请人： Christian Elend ,  Karl-Erich Jaeger ,  Christian Leggewie ,  Christel Vollstedt ,  Wolfgang Streit

    发明人： Christian Elend ,  Karl-Erich Jaeger ,  Christian Leggewie ,  Christel Vollstedt ,  Wolfgang Streit

    IPC分类号： C12P21/00 ,  C12N9/18 ,  C12N15/11 ,  C12N15/00 ,  C12N1/21 ,  C12N15/63 ,  C07K16/18 ,  C07H21/04 ,  C12P7/02

    CPC分类号： C12N9/18

    摘要： The present invention relates to a polynucleotide encoding an enzyme having carboxyl esterase [E.C. 3.1.1.1] activity, wherein the coding sequence is selected from the group consisting of (a) a polynucleotide encoding an amino acid sequence as depicted in any one of SEQ ID NOs: 2, 4, 6 and 8; (b) a polynucleotide having or comprising a nucleotide sequence encoding an enzyme, wherein the nucleic acid sequence is as shown in any one of SEQ ID NOs: 1, 3, 5 and 7; (c) a polynucleotide having or comprising a nucleotide sequence encoding a fragment or derivative of an enzyme encoded by a polynucleotide of (a) or (b), wherein in said derivative one or more amino acid residues are conservatively substituted compared to the amino acid sequence of (a); (d) a polynucleotide encoding an enzyme having carboxyl esterase activity which polynucleotide is at least 65% identical to a polynucleotide encoding an enzyme as shown in one of SEQ ID NOs: 2, 4, 6 and 8; (e) a polynucleotide having or comprising a nucleotide sequence the complementary strand of which hybridizes to a polynucleotide as defined in any one of (a) to (d); and (f) a polynucleotide having or comprising a nucleotide sequence being degenerate to the nucleotide sequence of the polynucleotide of (d) or (e); or the complementary strand of such a polynucleotide of (a) to (f) or fragments thereof useful as specific probes or primers. The present invention also relates to a host, genetically engineered with the polynucleotide of the present invention or the vector of the present invention. The present invention also relates to a polypeptide comprising the amino acid sequence encoded by a polynucleotide of the present invention or which is obtainable by the process of the present invention. Moreover, the present invention relates to a process for producing said polypeptide and for producing bacteria expressing said polypeptide. Finally, the present invention relates to a composition comprising the polynucleotide of the present invention, the vector of the present invention, the host of the present invention, the polypeptide of the present invention, the antibody of the present invention and/or one or more primers of the present invention.

    Method for registering the filling potential of a waste container of microplate washing devices
    10.
    发明授权
    Method for registering the filling potential of a waste container of microplate washing devices 有权
    用于记录微孔板洗涤装置的废物容器的填充电位的方法

    公开(公告)号：US08800365B2

    公开(公告)日：2014-08-12

    申请号：US13357773

    申请日：2012-01-25

    申请人： Wolfgang Streit ,  Juha Koota ,  Wolfgang Fuchs

    发明人： Wolfgang Streit ,  Juha Koota ,  Wolfgang Fuchs

    IPC分类号： G01F23/14 ,  G01N35/10 ,  G01F17/00 ,  B01L99/00

    CPC分类号： G01N35/1004 ,  B01L99/00 ,  B01L2300/0829 ,  G01F17/00 ,  G01F23/14

    摘要： Method for registering filling potential of a waste container of a microplate washing device has a needle for aspirating liquids, a waste container, a pump for generating a partial vacuum and a pressure sensor for determining air pressure in the container, a sensor controller, and a first valve for blocking the line between the needle and the waste container. The pump and needle are connected to the container and the method includes closing the first valve, generating a partial vacuum in a test range, closing a further valve between the container and the pump, opening the first valve, triggering a partial vacuum dissipation in the container, measuring a test time for the partial vacuum dissipation in the test range, comparing the test time to a known threshold time, and deciding that the waste container is fillable or not.

    摘要翻译： 用于记录微板清洗装置的废液容器的填充电位的方法具有用于吸液的针，废液容器，用于产生部分真空的泵和用于确定容器中的空气压力的压力传感器，传感器控制器和 用于阻塞针和废物容器之间的线的第一阀。 泵和针连接到容器，并且该方法包括关闭第一阀，在测试范围内产生部分真空，关闭容器和泵之间的另一阀，打开第一阀，触发第一阀中的部分真空耗散 在测试范围内测量部分真空耗散的测试时间，将测试时间与已知阈值时间进行比较，并确定废物容器是否可填充。