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公开(公告)号：US07618812B2
公开(公告)日：2009-11-17
申请号：US11191327
申请日：2005-07-28
IPC分类号： C12M1/34
CPC分类号： B01L3/502715 , B01F5/10 , B01F11/0071 , B01F13/0059 , B01F15/00123 , B01F15/00318 , B01F15/00396 , B01F15/0244 , B01F2015/00084 , B01F2215/0037 , B01F2215/0073 , B01J2219/00353 , B01J2219/00396 , B01J2219/00477 , B01J2219/00479 , B01J2219/00533 , B01J2219/00659 , B01L3/50273 , B01L3/502738 , B01L3/50855 , B01L9/06 , B01L2200/0689 , B01L2300/0636 , B01L2300/0822 , B01L2300/0877 , B01L2400/0481 , C40B60/14
摘要： A device provides a hybridization chamber for hybridizing nucleic acid samples, proteins or tissue sections on a slide, and is an essentially rectangular body movable opposite the slide. The device includes a surface, lines for supplying and/or removing media, a specimen supply line and an agitating device. The agitation device includes at least one membrane which separates a pressure chamer to be fillable with a pressure fluid via one of the lines, from at least one agitation chamber connected via an agitation line to the hybridization chamber. The lines are continuously open so the media therein can permanently flow unhindered. Four such devices can be combined into a process unit for providing a hybridization chamber. Such unit is pivotable around an axis and has a holder with four seats, which is lockable in relation to a baseplate, one device being insertable into each one of the seats.
摘要翻译： 一种装置提供用于将载体上的核酸样品，蛋白质或组织切片杂交的杂交室，并且是与载片相对移动的基本上矩形的主体。 该装置包括表面，用于供应和/或移除介质的管线，试样供应管线和搅拌装置。 所述搅拌装置包括至少一个膜，其通过所述管线将至少一个通过搅动管线连接的搅拌室与压力流体分离成压缩倒角，所述至少一个搅拌室通过所述杂交室。 线路是连续打开的，因此其中的介质可以永久地不受阻碍地流动。 四个这样的装置可以组合成用于提供杂交室的处理单元。 这种单元可围绕轴线枢转并且具有具有四个座的保持器，该座具可相对于底板锁定，一个装置可插入每个座中。
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公开(公告)号：US20060003440A1
公开(公告)日：2006-01-05
申请号：US11191327
申请日：2005-07-28
IPC分类号： C12M1/34
CPC分类号： B01L3/502715 , B01F5/10 , B01F11/0071 , B01F13/0059 , B01F15/00123 , B01F15/00318 , B01F15/00396 , B01F15/0244 , B01F2015/00084 , B01F2215/0037 , B01F2215/0073 , B01J2219/00353 , B01J2219/00396 , B01J2219/00477 , B01J2219/00479 , B01J2219/00533 , B01J2219/00659 , B01L3/50273 , B01L3/502738 , B01L3/50855 , B01L9/06 , B01L2200/0689 , B01L2300/0636 , B01L2300/0822 , B01L2300/0877 , B01L2400/0481 , C40B60/14
摘要： A device provides a hybridization chamber for hybridizing nucleic acid samples, proteins or tissue sections on a slide, and is an essentially rectangular body movable opposite the slide. The device includes a surface, lines for supplying and/or removing media, a specimen supply line and an agitating device. The agitation device includes at least one membrane which separates a pressure chamer to be fillable with a pressure fluid via one of the lines, from at least one agitation chamber connected via an agitation line to the hybridization chamber. The lines are continuously open so the media therein can permanently flow unhindered. Four such devices can be combined into a process unit for providing a hybridization chamber. Such unit is pivotable around an axis and has a holder with four seats, which is lockable in relation to a baseplate, one device being insertable into each one of the seats.
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3.发明授权Device for providing a hybridization chamber, and process unit and system for hybridizing nucleic acid samples, proteins, and tissue sections 失效用于提供杂交室的装置，以及用于杂交核酸样品，蛋白质和组织切片的处理单元和系统公开(公告)号：US06946287B2
公开(公告)日：2005-09-20
申请号：US10154467
申请日：2002-05-22
IPC分类号： G01N33/53 , B01F5/10 , B01F11/00 , B01F13/00 , B01F15/00 , B01L3/00 , B01L9/06 , C12M1/00 , C40B60/14 , G01N1/00 , G01N1/28 , G01N37/00 , C12M1/34
CPC分类号： B01L3/502715 , B01F5/10 , B01F11/0071 , B01F13/0059 , B01F15/00123 , B01F15/00318 , B01F15/00396 , B01F15/0244 , B01F2015/00084 , B01F2215/0037 , B01F2215/0073 , B01J2219/00353 , B01J2219/00396 , B01J2219/00477 , B01J2219/00479 , B01J2219/00533 , B01J2219/00659 , B01L3/50273 , B01L3/502738 , B01L3/50855 , B01L9/06 , B01L2200/0689 , B01L2300/0636 , B01L2300/0822 , B01L2300/0877 , B01L2400/0481 , C40B60/14
摘要： A device (1) is disclosed for providing a hybridization chamber (2), for hybridizing nucleic acid samples, proteins, or tissue sections on a slide (3), which is implemented so that it is movable in relation to this slide (3) and includes an annular sealing surface (4) for sealing the hybridization chamber (2) by being applied to a surface (5) of this slide (3), lines (6) for supplying and/or removing media to and/or from the hybridization chamber (2), and a specimen supply line (7). The devices according to the invention include a media-separating agitation device (8) for moving liquids in relation to samples immobilized on the surface (5) of the slide (3). Furthermore, process units (18) for hybridizing nucleic acid samples, proteins, or tissue sections and corresponding automatable systems including such process units (18) are disclosed.
摘要翻译： 公开了一种用于提供杂交室（2）的装置（1），用于将幻灯片（3）上的核酸样品，蛋白质或组织切片杂交，其实施为使得其相对于该载片（3）可移动， 并且包括用于通过施加到该滑动件（3）的表面（5）来密封杂交室（2）的环形密封表面（4），用于向和/或从 杂交室（2）和样品供应管线（7）。 根据本发明的装置包括用于相对于固定在载玻片（3）的表面（5）上的样品移动液体的介质分离搅拌装置（8）。 此外，公开了用于杂交核酸样品，蛋白质或组织切片的相应的可自动化系统（18）的工艺单元（18）。
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