利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

22 条记录 (耗时 0.047 秒)

    METHOD FOR DIRECT CAPTURE OF RIBONUCLEIC ACID
    11.
    发明申请
    METHOD FOR DIRECT CAPTURE OF RIBONUCLEIC ACID 审中-公开
    直接捕获RIBONUCLEIC ACID的方法

    公开(公告)号：US20100112643A1

    公开(公告)日：2010-05-06

    申请号：US12264462

    申请日：2008-11-04

    申请人： Marie J. Archer ,  Baochuan Lin ,  David A. Stenger

    发明人： Marie J. Archer ,  Baochuan Lin ,  David A. Stenger

    IPC分类号： C12P19/34 ,  C07H21/02

    CPC分类号： C40B50/18 ,  C12Q1/6813

    摘要： A method of: providing a solid surface having a dendrimer molecule bound thereto and a single-stranded probe nucleic acid immobilized to the dendrimer; contacting the solid surface with a sample suspected or known to contain a target ribonucleic acid; denaturing the target ribonucleic acid; and incubating the sample to allow hybridization of the denatured ribonucleic acid to the probe nucleic acids. The target ribonucleic acid is complementary to the probe nucleic acid.

    摘要翻译： 一种方法：提供具有结合到其上的树枝状大分子分子的固体表面和固定在树状聚合物上的单链探针核酸; 使固体表面与疑似或已知含有目标核糖核酸的样品接触; 使目标核糖核酸变性; 并培养样品以允许变性核糖核酸与探针核酸杂交。 靶核糖核酸与探针核酸互补。

    DESIGN AND SELECTION OF GENETIC TARGETS FOR SEQUENCE RESOLVED ORGANISM DETECTION AND IDENTIFICATION
    13.
    发明申请
    DESIGN AND SELECTION OF GENETIC TARGETS FOR SEQUENCE RESOLVED ORGANISM DETECTION AND IDENTIFICATION 失效
    设计和选择用于序列​​分解的有机体检测和鉴定的遗传学目标

    公开(公告)号：US20080033706A1

    公开(公告)日：2008-02-07

    申请号：US11843126

    申请日：2007-08-22

    申请人： Anthony Malanoski ,  Zheng Wang ,  Baochuan Lin ,  David Stenger ,  Joel Schnur

    发明人： Anthony Malanoski ,  Zheng Wang ,  Baochuan Lin ,  David Stenger ,  Joel Schnur

    IPC分类号： G06G7/48 ,  C40B40/08 ,  C40B50/02

    CPC分类号： C40B40/08 ,  G06F19/20

    摘要： A computer-implemented method as follows. Providing a list of target sequences associated with one or more organisms in a list of organisms. Providing a list of candidate prototype sequences suspected of hybridizing to one or more of the target sequences. Generating a collection of probes corresponding to each candidate prototype sequence, each collection of probes having a set of probes for every subsequence having a predetermined, fixed subsequence length of the corresponding candidate prototype sequence. The sets consist of the corresponding subsequence and every variation of the corresponding subsequence formed by varying a center nucleotide of the corresponding subsequence. Generating a set of fragments corresponding to each target sequence, each set of fragments having every fragment having a predetermined, fixed fragment length of the corresponding target sequence. Calculating the binding free energy of each fragment with a perfect complimentary sequence of the fragment. If any binding free energy is above a predetermined, fixed threshold, the fragment is extended one nucleotide at a time until the binding free energy is below the threshold or the fragment is the same length as the probe, generating a set of extended fragments. Determining which extended fragments are perfect matches to any of the probes. Assembling a base call sequence corresponding to each candidate prototype sequence. The base call sequence has a base call corresponding to the center nucleotide of each probe of the corresponding prototype sequence that is a perfect match to any extended fragment, but for which the other members of the set of probes containing the perfect match probe are not perfect matches to any extended fragment and a non-base call in all other circumstances.

    摘要翻译： 计算机实现的方法如下。 提供与生物体列表中的一种或多种生物相关的靶序列的列表。 提供疑似与一个或多个靶序列杂交的候选原型序列的列表。 产生与每个候选原型序列相对应的探针的集合，每个探针集合具有用于每个子序列的探针组，其具有相应候选原型序列的预定的固定子序列长度。 这些集由相应的子序列和通过改变相应子序列的中心核苷酸形成的相应子序列的每个变化组成。 产生对应于每个靶序列的一组片段，每组片段具有每个片段具有相应靶序列的预定的固定片段长度。 用片段的完美互补序列计算每个片段的结合自由能。 如果任何结合自由能高于预定的固定阈值，则片段一次延伸一个核苷酸，直到结合自由能低于阈值或片段与探针的长度相同，产生一组扩展片段。 确定哪些扩展片段与任何探针完美匹配。 组装对应于每个候选原型序列的基本调用序列。 基本调用序列具有对应于相应原型序列的每个探针的中心核苷酸的碱基调用，其与任何扩展片段完美匹配，但是包含完美匹配探针的探针组的其它成员对于其不是完美的 在任何其他情况下匹配任何扩展片段和非基本调用。

    COMPUTER-IMPLEMENTED BIOLOGICAL SEQUENCE IDENTIFIER SYSTEM AND METHOD
    14.
    发明申请
    COMPUTER-IMPLEMENTED BIOLOGICAL SEQUENCE IDENTIFIER SYSTEM AND METHOD 失效
    计算机实现生物学序列识别系统和方法

    公开(公告)号：US20070059728A1

    公开(公告)日：2007-03-15

    申请号：US11422431

    申请日：2006-06-06

    申请人： Anthony Malanoski ,  Baochuan Lin ,  Joel Schnur ,  David Stenger

    发明人： Anthony Malanoski ,  Baochuan Lin ,  Joel Schnur ,  David Stenger

    IPC分类号： C12Q1/68 ,  G06F19/00 ,  C12M1/34

    CPC分类号： G06F19/22 ,  G06F19/18 ,  G06F19/20 ,  Y10S707/99936

    摘要： A method of: submitting reference sequences to a taxonomic database to produce taxonomic results; and reporting a taxonomic identification based on the taxonomic results. The reference sequences are the output of genetic database queries that return a score for each reference sequence. A method for processing a biological sequence obtained from an assay by: converting base calls located in a predetermined list of positions within the biological sequence to N; and determining the ratio of single nucleotide polymorphisms in the biological sequence relative to a reference sequence. Each entry in the predetermined list of positions represents the capability of a substance hybridizing to a microarray used to generate the biological sequence. The substance is not the nucleic acid of a target pathogen.

    摘要翻译： 一种方法：将参考序列提交给分类数据库以产生分类结果; 并根据分类结果报告分类鉴定。 参考序列是返回每个参考序列分数的遗传数据库查询的输出。 一种用于处理通过以下方式获得的生物学序列的方法：将位于所述生物学序列内的预定位置列表中的基本呼叫转换为N; 并确定生物序列中单核苷酸多态性相对于参考序列的比例。 预定位置列表中的每个条目表示与用于产生生物序列的微阵列杂交的物质的能力。 该物质不是目标病原体的核酸。

    Large scale preparation method for functionalizing the surface of magnetic microparticles with an inorganic phosphorous dendrimer
    15.
    发明申请
    Large scale preparation method for functionalizing the surface of magnetic microparticles with an inorganic phosphorous dendrimer 有权
    用无机磷树枝状聚合物对磁性微粒表面进行官能化的大规模制备方法

    公开(公告)号：US20140275628A1

    公开(公告)日：2014-09-18

    申请号：US14208265

    申请日：2014-03-13

    申请人： Marie J. Archer ,  Baochuan Lin

    发明人： Marie J. Archer ,  Baochuan Lin

    IPC分类号： C07F9/6593

    CPC分类号： C07F9/65815

    摘要： A method of attaching a phosphorous dendrimer onto magnetic microparticles by taking magnetic microparticles in a water-based solution, then performing a solvent exchange, then suspending the microparticles in a phosphorous dendrimer solution and shaking, then washing the microparticles with an organic solvent, and then washing the microparticles with a transition solvent. The solvent exchange is done by washing the microparticles with a first concentration of a transition solvent, then washing the microparticles with a second concentration of the transition solvent where the second concentration is greater than the first concentration, then washing the microparticles with an organic solvent, then washing the microparticles with the transition solvent, then washing the microparticles with the organic solvent, and then suspending the microparticles in the transition solvent. Also disclosed is the related phosphorous dendrimer made by this method.

    摘要翻译： 通过将水性溶液中的磁性微粒附着到磁性微粒上，然后进行溶剂交换，然后将微粒悬浮在磷树枝状聚合物溶液中并振荡，然后用有机溶剂洗涤微粒，然后再将该磷树枝状聚合物 用过渡溶剂洗涤微粒。 通过用第一浓度的过渡溶剂洗涤微粒，然后用第二浓度的第二浓度的过渡溶剂洗涤微粒，其中第二浓度大于第一浓度，然后用有机溶剂洗涤微粒来进行溶剂交换， 然后用过渡溶剂洗涤微粒，然后用有机溶剂洗涤微粒，然后将微粒悬浮在过渡溶剂中。 还公开了通过该方法制备的相关的磷树枝状聚合物。

    Large scale preparation method for functionalizing the surface of magnetic microparticles with an inorganic phosphorous dendrimer
    18.
    发明授权
    Large scale preparation method for functionalizing the surface of magnetic microparticles with an inorganic phosphorous dendrimer 有权
    用无机磷树枝状聚合物对磁性微粒表面进行官能化的大规模制备方法

    公开(公告)号：US09296771B2

    公开(公告)日：2016-03-29

    申请号：US14208265

    申请日：2014-03-13

    申请人： Marie J. Archer ,  Baochuan Lin

    发明人： Marie J. Archer ,  Baochuan Lin

    IPC分类号： C40B50/12 ,  A61K8/24 ,  C07F9/6593

    CPC分类号： C07F9/65815

    摘要： A method of attaching a phosphorous dendrimer onto magnetic microparticles by taking magnetic microparticles in a water-based solution, then performing a solvent exchange, then suspending the microparticles in a phosphorous dendrimer solution and shaking, then washing the microparticles with an organic solvent, and then washing the microparticles with a transition solvent. The solvent exchange is done by washing the microparticles with a first concentration of a transition solvent, then washing the microparticles with a second concentration of the transition solvent where the second concentration is greater than the first concentration, then washing the microparticles with an organic solvent, then washing the microparticles with the transition solvent, then washing the microparticles with the organic solvent, and then suspending the microparticles in the transition solvent. Also disclosed is the related phosphorous dendrimer made by this method.

    摘要翻译： 通过将水性溶液中的磁性微粒附着到磁性微粒上，然后进行溶剂交换，然后将微粒悬浮在磷树枝状聚合物溶液中并振荡，然后用有机溶剂洗涤微粒，然后再将该磷树枝状聚合物 用过渡溶剂洗涤微粒。 通过用第一浓度的过渡溶剂洗涤微粒，然后用第二浓度的第二浓度的过渡溶剂洗涤微粒，其中第二浓度大于第一浓度，然后用有机溶剂洗涤微粒来进行溶剂交换， 然后用过渡溶剂洗涤微粒，然后用有机溶剂洗涤微粒，然后将微粒悬浮在过渡溶剂中。 还公开了通过该方法制备的相关的磷树枝状聚合物。