Rice and products thereof having starch with an increased proportion of amylose

    公开(公告)号:US07790955B2

    公开(公告)日:2010-09-07

    申请号:US10577564

    申请日:2004-10-27

    摘要: Rice having reduced levels of starch branching enzymes produce grain having a high relative amylose content in the endosperm. The rice grain of this invention can be of a non-shrunken phenotype despite a lesion in the amylopectin synthesis pathway and may be transgenic or nontransgenic.    1 GCCACCGACA TCCGCCGCAA TGCTGTGTCT CACCTCCTCT TCCTCCTCCG CGCCCGCTCC   61 GCTCCTTCCC TCTCTCGCTG ATCGACCGAG CCCGGGAATC GCGGGCGGGG GTGGCAATGT  121 TCGCCTGAGC GTGGTTTCTT CGCCGCGCCG GTCGTGGCCT GGAAAGGTCA AGACCAATTT  181 CTCAGTTCCT GCGACTGCGC GAAAAAACAA AACCATGGTG ACTGTTGTGG AGGAGGTCGA  241 CCACCTTCCT ATATATGATC TGGACCCTAA GTTGGAGGAA TTCAAGGATC ACTTCAACTA  301 TAGGATAAAA AGATACCTCG ACCAGAAATG CCTGATTGAA AAACATGAGG GGGGCCTTGA  361 AGAATTTTCT AAAGGCTATT TGAAGTTTGG GATTAATACA GTTGATGGTG CCACAATATA  421 TCGTGAATGG GCGCCTGCTG CACAAGAAGC ACAGCTCATT GGTGAGTTCA ATAACTGGAA  481 TGGTGCAAAA CACAAGATGG AGAAGGATAA ATTTGGCATT TGGTCAATCA AGATTTCACA  541 TGTCAATGGG AAGCCTGCCA TCCCTCACAA TTCCAAGGTT AAATTTCGCT TTAGGCATGG  601 GGGTGGAGCA TGGGTTGATC GTATTCCCGC ATGGATTCGT TATGCAACTT TTGATGCCTC  661 TAAATTTGGA GCTCCATATG ATGGTGTACA CTGGGATCCT CCAGCCTGTG AAAGGTACGT  721 GTTTAAGCAT CCTCGACCTC CAAAACCTGA TGCTCCACGC ATCTATGAGG CTCATGTGGG  781 GATGAGTGGT GAAGAGCCAG AAGTAAGCAC ATACAGAGAA TTTGCAGACA ATGTGTTACC  841 ACGCATACGG GCAAATAACT ACAACACAGT TCAGTTAATG GCAATCATGG AACATTCCTA  901 CTATGCTTCT TTTGGGTATC ACGTGACAAA TTTTTTCGCA GTCAGCAGCA GATCAGGAAC  961 ACCAGAGGAT CTGAAATATC TTGTTGACAA GGCACATAGT TTAGGATTAC GAGTTCTGAT 1021 GGATGTTGTC CATAGCCATG CGAGTAATAA TGTGACCGAT GGTCTAAATG GCTATGACGT 1081 TGGACAAAAC ACTCATGAGT CTTATTTTCA TACAGGAGAT AGGGGCTACC ATAAACTCTG 1141 GGATAGTCGT CTGTTCAACT ATGCCAATTG GGAGGTCTTA AGATTTCTTC TTTCTAATTT 1201 GAGATATTGG ATGGACGAAT TCATGTTTGA TGGCTTCCGA TTTGATGGGG TTACATCAAT 1261 GCTATACCAT CACCATGGTA TCAATAAGGG ATTTACTGGA AACTACAAGG AGTATTTCAG 1321 TTTGGATACC GATGTGGATG CAATTGTTTA CATGATGCTC GCAAACCATT TAATGCATAA 1381 ACTCTTGCCG GAAGCAACTA TTGTTGCTGA AGATGTTTCG GGCATGCCAG TGCTTTGTCG 1441 GCCAGTTGAT GAAGGTGGAG TAGGGTTTGA CTTCCGCCTG GCAATGGCCA TTCCTGATAG 1501 ATGGATTGAC TACCTGAAGA ACAAAGAGGA CCGCAAATGG TCAATGAGTG AAATAGTGCA 1561 AACTTTGACT AACAGGAGAT ATACAGAAAA ATGCATTGCC TATGCCGAGA GCCATGATCA 1621 GTCCATTGTT GGTGACAAGA CTATAGCATT TCTCTTGATG GACAAGGAAA TGTACACTGG 1681 CATGTCAGAC TTGCAGCCTG CTTCACCTAC CATCAACCGT GGCATTGCAC TCCAAAAGAT 1741 GATTCACTTC ATTACGATGG CCCTTGGAGG TGATGGCTAC TTAAATTTTA TGGGCAATGA 1801 GTTTGGCCAT CCAGAATGGA TTGACTTTCC AAGAGAAGGC AACAACTGGA GCTATGATAA 1861 ATGCAGACGT CAGTGGAGCC TTGTCGACAC TGATCACCTT CGATACAAGT ATATGAATGC 1921 ATTTGATCAA GCAATGAATG CACTCGAGGA GGAATTTTCC TTCCTGTCAT CATCAAAGCA 1981 GATTGTTAGC GACATGAACG AGAAAGATAA GGTTATTGTC TTTGAACGTG GAGATTTGGT 2041 TTTTGTTTTC AATTTTCATC CCAACAAAAC TTATAAGGGT TACAAAGTCG GATGTGACTT 2101 GCCCGGGAAG TACAGAGTAG CTCTGGACTG TGATGCTTTG GTCTTTGGTG GCCATGGAAG 2161 AGTTGGCCAT GATGTGGATC ACTTCACGTC TCCCGAGGGA ATGCCAGGAG TACCAGAAAC 2221 AAATTTCAAC AACCGCCCTA ACTCATTCAA AGTCCTTTCC CCGCCCCGTA CCTGTGTGGC 2281 TTACTATCGC GTTGATGAAG ATCGTGAAGA GCTCAGGAGG GGTGGAGCAG TTGCTTCTGG 2341 AAAGATTGTT ACAGAGTATA TCGATGTTGA AGCAACAAGT GGGGAGACTA TCTCTGGTGG 2401 CTGGAAGGGC TCCGAGAAGG ACGATTGTGG CAAGAAAGGG ATGAAGTTTG TGTTTCGGTC 2461 TTCTGACGAA GACTGCAAAT GAAGCATCAG ATTTCTTGAT CAGGAGCAAC TGTTGGTGCC 2521 CTTGTAATCT GGAGATCCTG GCTTGCCTTG GACTTGGTTG TGGTTCTTTA GCAGTTGCTA 2581 TGTACCTATC TATGATATGA ACTTTATGTA TAGTTCGCCT TAAAGAAAGA ATAAGCAGTG 2641 ATGATGTGGC CTTAAACCTG AGCTGCACAA GCCTAATGTA AAAATAAAGT TTCAGGCTTT 2701 CATCCAGAAT AAAACAGCTG TTCATTTACC ATCTCAAAA

    Novel isoamylases and associated methods and products
    19.
    发明申请
    Novel isoamylases and associated methods and products 审中-公开
    新型异戊基酶及相关方法和产品

    公开(公告)号:US20060015965A1

    公开(公告)日:2006-01-19

    申请号:US10495662

    申请日:2002-11-12

    IPC分类号: C12Q1/68 A01H1/00 C12N15/82

    摘要: The present invention provides methods for distinguishing between different isoamylase genes of individual genomes of a polyploid plant. These methods enable a polyploid plant-to be produced and/or identified which has at least one impaired isoamylase gene. In particular, the methods of the invention enable various plants of a polyploidy species to be produced and/or indentified which, between the plants, lack a functional isoamylase gene for each genome of the plant. Such plants can be cross-bred to produce a plant which lacks any functional isoamylase protein. Furthermore, the invention provides numerous isoamylase gene, protein and cDNA sequences from various polyploidy plant species, including hexaploid wheat.

    摘要翻译: 本发明提供了用于区分多倍体植物的个体基因组的不同异淀粉酶基因的方法。 这些方法能够产生和/或鉴定具有至少一种受损的异淀粉酶基因的多倍体植物。 特别地,本发明的方法能够产生和/或识别多倍体物种的各种植物,其在植物之间缺少植物的每个基因组的功能性异淀粉酶基因。 这样的植物可以交叉繁殖以产生缺乏任何功能性异淀粉酶蛋白质的植物。 此外,本发明提供了来自各种多倍体植物物种的许多异淀粉酶基因,蛋白质和cDNA序列,包括六倍体小麦。