公开(公告)号：US20170067064A1

公开(公告)日：2017-03-09

申请号：US15182839

申请日：2016-06-15

Applicant: BASF SE

Inventor： Cornelius Bessler ,  Jorg Feesche ,  Stefan Evers ,  Karl-Heinz Maurer ,  Armin Ehrenreich ,  Birgit Veith ,  Heiko Liesegang ,  Anke Henne ,  Christina Herzberg ,  Gerhard Gottschalk

IPC: C12N15/52 ,  C12P7/42 ,  C12P7/52 ,  C12P13/00 ,  C12N15/75 ,  C12N9/84 ,  C12N9/88 ,  C12N9/04 ,  C12N9/02 ,  C12N9/00 ,  C12N9/12 ,  C12P7/16 ,  C12N9/10

CPC classification number: C12N15/52 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/001 ,  C12N9/0016 ,  C12N9/1025 ,  C12N9/1029 ,  C12N9/1096 ,  C12N9/1217 ,  C12N9/16 ,  C12N9/78 ,  C12N9/80 ,  C12N9/84 ,  C12N9/88 ,  C12N9/93 ,  C12N15/75 ,  C12P7/16 ,  C12P7/40 ,  C12P7/42 ,  C12P7/52 ,  C12P13/001 ,  C12Y101/01 ,  C12Y101/01035 ,  C12Y101/01157 ,  C12Y102/04002 ,  C12Y103/99 ,  C12Y203/01019 ,  C12Y206/01042 ,  C12Y207/02007 ,  C12Y305/01001 ,  C12Y305/01011 ,  C12Y401/01018 ,  C12Y401/01019 ,  C12Y402/01017 ,  C12Y402/01055 ,  C12Y602/01 ,  C12Y602/01001 ,  Y02E50/10 ,  Y02P20/52

Abstract: The present invention relates to 25 hitherto undescribed genes of B. licheniformis and gene products derived thereform and all sufficiently homologous nucleic acids and proteins thereof. They occur in five different metabolic pathways for the formation of odorous substances. The metabolic pathways in question are for the synthesis of: 1) isovalerian acid (as part of the catabolism of leucine), 2) 2-methylbutyric acid and/or isobutyric acid (as part of the catabolism of valine and/or isoleucine), 3) butanol and/or butyric acid (as part of the metabolism of butyric acid), 4) propyl acid (as part of the metabolism of propionate) and/or 5) cadaverine and/or putrescine (as parts of the catabolism of lysine and/or arginine). The identification of these genes allows biotechnological production methods to be developed that are improved to the extent that, to assist these nucleic acids, the formation of the odorous substances synthesised via these metabolic pathways can be reduced by deactivating the corresponding genes in the micro-organism used for the biotechnological production. In addition, these gene products are thus available for preparing reactions or for methods according to their respective biochemical properties.

Abstract translation: 本发明涉及地衣芽孢杆菌的25个迄今未描述的基因以及衍生自地衣芽孢杆菌的基因产物及其全部充分同源的核酸及其蛋白质。 它们发生在五种不同的代谢途径中，用于形成气味物质。 所讨论的代谢途径是用于合成：1）异戊酸（作为亮氨酸分解代谢的一部分），2）2-甲基丁酸和/或异丁酸（作为缬氨酸和/或异亮氨酸分解代谢的一部分） 3）丁醇和/或丁酸（作为丁酸代谢的一部分），4）丙酸（作为丙酸代谢的一部分）和/或5）尸胺和/或腐胺（作为赖氨酸分解代谢的一部分） 和/或精氨酸）。 这些基因的鉴定允许开发生物技术生产方法，其被改进到对于辅助这些核酸的程度，可以通过使微生物中相应的基因失活来减少通过这些代谢途径合成的恶臭物质的形成 用于生物技术生产。 此外，这些基因产物因此可用于制备反应或根据其各自生物化学性质的方法。

公开(公告)号：US09353361B2

公开(公告)日：2016-05-31

申请号：US14300826

申请日：2014-06-10

Applicant: BASF SE

Inventor： Cornelius Bessler ,  Susanne Wieland ,  Karl-Heinz Maurer

IPC: C12N15/56 ,  C12N9/28 ,  C11D3/386

CPC classification number: C12N9/2417 ,  C11D3/38681

Abstract: The present invention relates to α-amylase variants that are stabilized to dimerization and/or multimerization, in particular at elevated temperatures or high pH, by point mutagenesis of positively polarized or charged or neutral surface amino acids to give more negatively polarized or charged amino acids. The invention further relates to methods of increasing the stability of an α-amylase to dimerization and/or multimerization brought about by electrostatic interactions whereby at least one amino acid residue on the surface of the starting molecule, which makes a neutral or positively polar or charged contribution to the electrostatic potential of said molecule, is replaced with a more negatively polar or negatively charged amino acid residue. The α-amylase variants obtained thereby exhibit better stability to influences of the solvent, increased processivity, and are suited for numerous industrial areas of use, in particular as active ingredients in detergents and cleansers.

Abstract translation: 本发明涉及通过正极化或带电荷或中性表面氨基酸的点诱变稳定至二聚化和/或多聚化的α-淀粉酶变体，特别是在升高的温度或高pH下，以产生更多的负极化或带电荷的氨基酸 。 本发明还涉及增加α-淀粉酶对由静电相互作用引起的二聚化和/或多聚化的稳定性的方法，其中起始分子表面上至少有一个氨基酸残基，其形成中性或正极性或带电 对所述分子的静电电位的贡献被更负极性或带负电荷的氨基酸残基取代。 由此获得的α-淀粉酶变体对溶剂的影响表现出更好的稳定性，增加的持续性，并且适用于许多工业使用领域，特别是作为洗涤剂和清洁剂中的活性成分。