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公开(公告)号：US09957531B1
公开(公告)日：2018-05-01
申请号：US15658668
申请日：2017-07-25
发明人： Michael Koepke , Rasmus Overgaard Jensen , James Bruce Yarnton Haycock Behrendorff , Ryan Edward Hill , Darmawi Juminaga , Alexander Paul Mueller
IPC分类号： C12N1/20 , C12P7/42 , C12N9/10 , C12N9/02 , C12N9/04 , C12N9/12 , C12N9/16 , C12P7/24 , C12P7/18
CPC分类号： C12P7/42 , C12N9/0006 , C12N9/0008 , C12N9/1029 , C12N9/1217 , C12N9/16 , C12P5/007 , C12P5/026 , C12P7/04 , C12P7/18 , C12P7/24 , C12P7/28 , C12P7/625 , C12Y101/01001 , C12Y101/01002 , C12Y102/07005 , C12Y203/01008 , C12Y203/01009 , C12Y203/01019 , C12Y207/02001 , C12Y207/02007 , C12Y301/0202
摘要： The invention relates to a genetically engineered bacterium having an enzyme that converts acetyl-CoA to acetoacetyl-CoA, an enzyme that converts acetoacetyl-CoA to 3-hydroxybutyryl-CoA, and an enzyme that converts 3-hydroxybutyryl-CoA to 3-hydroxybutyrate. The bacterium may also have enzymes to produce other downstream products, such as 3-hydroxybutyryaldehyde, and 1,3-butanediol. Typically, the bacterium is capable of producing these products from a gaseous substrate, such as syngas or an industrial waste gas.
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公开(公告)号：US20170253896A1
公开(公告)日：2017-09-07
申请号：US15511684
申请日：2015-09-16
CPC分类号： C12P7/52 , C12N9/1029 , C12N9/1217 , C12N9/88 , C12N15/52 , C12Y203/01008 , C12Y203/01019 , C12Y207/02001 , C12Y207/02007 , C12Y207/02014 , C12Y207/02015 , C12Y402/01017 , C12Y402/01018 , C12Y402/01055 , C12Y402/01059 , C12Y402/01074 , C12Y402/01116
摘要： Described is a method for the conversion of 3-methylcrotonyl-CoA into 3-hydroxy-3-methylbutyric acid comprising the steps of:(a) enzymatically converting 3-methylcrotonyl-CoA into 3-hydroxy-3-methylbutyryl-CoA; and (b) further enzymatically converting the thus produced 3-hydroxy-3-methylbutyryl-CoA into 3-hydroxy-3-methylbutyric acid wherein the enzymatic conversion of 3-hydroxy-3-methylbutyryl-CoA into 3-hydroxy-3-methylbutyric acid according to step (b) is achieved by first converting 3-hydroxy-3-methylbutyryl-CoA into 3-hydroxy-3-methylbutyryl phosphate and then subsequently converting the thus produced 3-hydroxy-3-methylbutyryl phosphate into 3-hydroxy-3-methylbutyric acid.
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3.发明申请公开(公告)号：US20070190605A1
公开(公告)日：2007-08-16
申请号：US11616319
申请日：2006-12-27
申请人： Cornelius Bessler , Jorg Feesche , Stefan Evers , Karl-Heinz Maurer , Armin Ehrenreich , Birgit Veith , Heiko Liesegang , Anke Henne , Christina Herzberg , Gerhard Gottschalk
发明人： Cornelius Bessler , Jorg Feesche , Stefan Evers , Karl-Heinz Maurer , Armin Ehrenreich , Birgit Veith , Heiko Liesegang , Anke Henne , Christina Herzberg , Gerhard Gottschalk
IPC分类号： C07H21/04 , C12P21/06 , C12P7/40 , C12N9/02 , C12N9/16 , C12N9/32 , C12N9/42 , C12N9/50 , C12N9/64 , C12N15/74 , C12N1/21
CPC分类号： C12N15/52 , C12N9/0006 , C12N9/0008 , C12N9/001 , C12N9/0016 , C12N9/1025 , C12N9/1029 , C12N9/1096 , C12N9/1217 , C12N9/16 , C12N9/78 , C12N9/80 , C12N9/84 , C12N9/88 , C12N9/93 , C12N15/75 , C12P7/16 , C12P7/40 , C12P7/42 , C12P7/52 , C12P13/001 , C12Y101/01 , C12Y101/01035 , C12Y101/01157 , C12Y102/04002 , C12Y103/99 , C12Y203/01019 , C12Y206/01042 , C12Y207/02007 , C12Y305/01001 , C12Y305/01011 , C12Y401/01018 , C12Y401/01019 , C12Y402/01017 , C12Y402/01055 , C12Y602/01 , C12Y602/01001 , Y02E50/10 , Y02P20/52
摘要： The present invention relates to 25 hitherto undescribed genes of B. licheniformis and gene products derived therefrom and all sufficiently homologous nucleic acids and proteins thereof. They occur in five different metabolic pathways for the formation of odorous substances. The metabolic pathways in question are for the synthesis of: 1) isovalerian acid (as part of the catabolism of leucine), 2) 2-methylbutyric acid and/or isobutyric acid (as part of the catabolism of valine and/or isoleucine), 3) butanol and/or butyric acid (as part of the metabolism of butyric acid), 4) propyl acid (as part of the metabolism of propionate) and/or 5) cadaverine and/or putrescine (as parts of the catabolism of lysine and/or arginine). The identification of these genes allows biotechnological production methods to be developed that are improved to the extent that, to assist these nucleic acids, the formation of the odorous substances synthesized via these metabolic pathways can be reduced by deactivating the corresponding genes in the micro-organism used for the biotechnological production. In addition, these gene products are thus available for preparing reactions or for methods according to their respective biochemical properties.
摘要翻译： 本发明涉及地衣芽孢杆菌的25个迄今未描述的基因及其衍生的基因产物及其全部充分同源的核酸及其蛋白质。 它们发生在五种不同的代谢途径中，用于形成气味物质。 所讨论的代谢途径是用于合成：1）异戊酸（作为亮氨酸分解代谢的一部分），2）2-甲基丁酸和/或异丁酸（作为缬氨酸和/或异亮氨酸分解代谢的一部分） 3）丁醇和/或丁酸（作为丁酸代谢的一部分），4）丙酸（作为丙酸代谢的一部分）和/或5）尸胺和/或腐胺（作为赖氨酸分解代谢的一部分） 和/或精氨酸）。 这些基因的鉴定允许开发生物技术生产方法，其被改进到对于辅助这些核酸的程度，可以通过使微生物中相应的基因失活来减少通过这些代谢途径合成的恶臭物质的形成 用于生物技术生产。 此外，这些基因产物因此可用于制备反应或根据其各自生物化学性质的方法。
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公开(公告)号：US09738875B2
公开(公告)日：2017-08-22
申请号：US15293191
申请日：2016-10-13
发明人： Michael Koepke , Rasmus Overgaard Jensen , James Bruce Yarnton Haycock Behrendorff , Ryan Edward Hill
CPC分类号： C12P7/42 , C12N9/0006 , C12N9/0008 , C12N9/1029 , C12N9/1217 , C12N9/16 , C12P5/007 , C12P5/026 , C12P7/04 , C12P7/18 , C12P7/24 , C12P7/28 , C12P7/625 , C12Y101/01001 , C12Y101/01002 , C12Y102/07005 , C12Y203/01008 , C12Y203/01009 , C12Y203/01019 , C12Y207/02001 , C12Y207/02007 , C12Y301/0202
摘要： The invention relates to a genetically engineered bacterium comprising an energy-generating fermentation pathway and methods related thereto. In particular, the invention provides a bacterium comprising a phosphate butyryltransferase (Ptb) and a butyrate kinase (Buk) (Ptb-Buk) that act on non-native substrates to produce a wide variety of products and intermediates. In certain embodiments, the invention relates to the introduction of Ptb-Buk into a C1-fixing microoorgansim capable of producing products from a gaseous substrate.
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5.发明申请Bacteria engineered to treat diseases that benefit from reduced gut inflammation and/or tighten gut mucosal barrier 审中-公开用于治疗受益于减少肠道炎症和/或收紧肠粘膜屏障的疾病的细菌公开(公告)号：US20160206666A1
公开(公告)日：2016-07-21
申请号：US14998376
申请日：2015-12-22
申请人： Synlogic, Inc.
CPC分类号： C12N15/70 , A61K31/19 , A61K31/198 , A61K35/741 , A61K38/20 , A61K38/2013 , A61K38/2066 , A61K38/26 , A61K38/446 , A61K2035/115 , C12N9/001 , C12N9/1217 , C12Y103/08001 , C12Y115/01001 , C12Y207/02007 , Y02A50/401 , Y02A50/414 , Y02A50/473
摘要： Genetically engineered bacteria, pharmaceutical compositions thereof, and methods of treating or preventing autoimmune disorders, inhibiting inflammatory mechanisms in the gut, and/or tightening gut mucosal barrier function are disclosed.
摘要翻译： 公开了遗传工程细菌，其药物组合物，以及治疗或预防自身免疫疾病，抑制肠道炎症机制和/或收紧肠粘膜屏障功能的方法。
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公开(公告)号：US20130217086A1
公开(公告)日：2013-08-22
申请号：US13725256
申请日：2012-12-21
发明人： Young Min LEE , Hyun Min KOO , Jae Young KIM , Jin Woo KIM , Jae Chan PARK , Hwa Young CHO , Joon Song PARK , Pyung Cheon LEE
IPC分类号： C12P7/18
CPC分类号： C12P7/18 , C12N9/0006 , C12N9/0008 , C12N9/1029 , C12N9/1217 , C12N9/13 , C12N9/88 , C12Y101/01001 , C12Y101/01016 , C12Y102/01016 , C12Y102/04002 , C12Y203/01019 , C12Y207/02007 , C12Y208/03005 , C12Y401/01071
摘要： A modified microorganism for production of 1,4-butanediol, an expression vector, and a method of producing 1,4-butanediol using the modified microorganism are provided. The method can be useful in producing 1,4-butanediol using a biological production process.
摘要翻译： 提供了用于生产1,4-丁二醇的改性微生物，表达载体，以及使用该修饰微生物生产1,4-丁二醇的方法。 该方法可用于使用生物制备方法生产1,4-丁二醇。
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公开(公告)号：US20180105847A1
公开(公告)日：2018-04-19
申请号：US15658668
申请日：2017-07-25
发明人： Michael Koepke , Rasmus Overgaard Jensen , James Bruce Yarnton Haycock Behrendorff , Ryan Edward Hill , Darmawi Juminaga , Alexander Paul Mueller
CPC分类号： C12P7/42 , C12N9/0006 , C12N9/0008 , C12N9/1029 , C12N9/1217 , C12N9/16 , C12P5/007 , C12P5/026 , C12P7/04 , C12P7/18 , C12P7/24 , C12P7/28 , C12P7/625 , C12Y101/01001 , C12Y101/01002 , C12Y102/07005 , C12Y203/01008 , C12Y203/01009 , C12Y203/01019 , C12Y207/02001 , C12Y207/02007 , C12Y301/0202
摘要： The invention relates to a genetically engineered bacterium having an enzyme that converts acetyl-CoA to acetoacetyl-CoA, an enzyme that converts acetoacetyl-CoA to 3-hydroxybutyryl-CoA, and an enzyme that converts 3-hydroxybutyryl-CoA to 3-hydroxybutyrate. The bacterium may also have enzymes to produce other downstream products, such as 3-hydroxybutyryaldehyde, and 1,3-butanediol. Typically, the bacterium is capable of producing these products from a gaseous substrate, such as syngas or an industrial waste gas.
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8.发明授权Recombinant microorganism having enhanced butanol producing ability and method for producing butanol using the same 有权具有提高的丁醇生产能力的重组微生物及使用其生产丁醇的方法公开(公告)号：US09567613B2
公开(公告)日：2017-02-14
申请号：US14418401
申请日：2013-03-11
发明人： Sang-Hyun Lee , Moon-Ho Eom
CPC分类号： C12P7/16 , C12N9/0006 , C12N9/0008 , C12N9/1029 , C12N9/1217 , C12N9/13 , C12N15/52 , C12Y101/01001 , C12Y102/01003 , C12Y203/01008 , C12Y207/02007 , C12Y208/03008 , Y02E50/10 , Y02P20/52
摘要： The present invention relates to a microorganism having an acetyl CoA biosynthesis pathway and a butyryl CoA biosynthesis pathway; the microorganism being a recombinant microorganism having an increased ability to produce butanol, wherein a pathway for converting acetyl CoA into acetate is suppressed, and a pathway for converting acetate into acetyl CoA and a pathway for converting butyryl CoA into butanol are promoted. Also, the present invention concerns a method for producing butanol by using the recombinant microorganism.
摘要翻译： 本发明涉及具有乙酰CoA生物合成途径和丁酰辅酶A生物合成途径的微生物; 所述微生物是具有增加生产丁醇能力的重组微生物，其中抑制将乙酰CoA转化为乙酸的途径，并且促进将乙酸转化为乙酰CoA的途径和将丁酰CoA转化为丁醇的途径。 另外，本发明涉及使用重组微生物生产丁醇的方法。
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9.发明授权Gene products of Bacillus licheniformis which form odorous substances and improved biotechnological production methods based thereon 有权其形成气味物质的地衣芽孢杆菌的基因产物和基于其的改良的生物技术生产方法公开(公告)号：US09394528B2
公开(公告)日：2016-07-19
申请号：US11616319
申请日：2006-12-27
申请人： Cornelius Bessler , Jörg Feesche , Stefan Evers , Karl-Heinz Maurer , Armin Ehrenreich , Birgit Veith , Heiko Liesegang , Anke Henne , Christina Herzberg , Gerhard Gottschalk
发明人： Cornelius Bessler , Jörg Feesche , Stefan Evers , Karl-Heinz Maurer , Armin Ehrenreich , Birgit Veith , Heiko Liesegang , Anke Henne , Christina Herzberg , Gerhard Gottschalk
IPC分类号： C12N15/74 , C12N1/20 , C12P21/06 , C12N9/06 , C12N9/04 , C12N9/02 , C12N9/10 , C12N9/12 , C12N9/16 , C12N9/78 , C12N9/80 , C12N9/88 , C12N9/00 , C12N15/52 , C12P7/16 , C12P7/40 , C12P7/52 , C12P13/00
CPC分类号： C12N15/52 , C12N9/0006 , C12N9/0008 , C12N9/001 , C12N9/0016 , C12N9/1025 , C12N9/1029 , C12N9/1096 , C12N9/1217 , C12N9/16 , C12N9/78 , C12N9/80 , C12N9/84 , C12N9/88 , C12N9/93 , C12N15/75 , C12P7/16 , C12P7/40 , C12P7/42 , C12P7/52 , C12P13/001 , C12Y101/01 , C12Y101/01035 , C12Y101/01157 , C12Y102/04002 , C12Y103/99 , C12Y203/01019 , C12Y206/01042 , C12Y207/02007 , C12Y305/01001 , C12Y305/01011 , C12Y401/01018 , C12Y401/01019 , C12Y402/01017 , C12Y402/01055 , C12Y602/01 , C12Y602/01001 , Y02E50/10 , Y02P20/52
摘要： The present invention relates to 25 hitherto undescribed genes of B. licheniformis and gene products derived therefrom and all sufficiently homologous nucleic acids and proteins thereof. They occur in five different metabolic pathways for the formation of odorous substances. The metabolic pathways in question are for the synthesis of: 1) isovalerian acid (as part of the catabolism of leucine), 2) 2-methylbutyric acid and/or isobutyric acid (as part of the catabolism of valine and/or isoleucine), 3) butanol and/or butyric acid (as part of the metabolism of butyric acid), 4) propyl acid (as part of the metabolism of propionate) and/or 5) cadaverine and/or putrescine (as parts of the catabolism of lysine and/or arginine). The identification of these genes allows biotechnological production methods to be developed that are improved to the extent that, to assist these nucleic acids, the formation of the odorous substances synthesized via these metabolic pathways can be reduced by deactivating the corresponding genes in the micro-organism used for the biotechnological production. In addition, these gene products are thus available for preparing reactions or for methods according to their respective biochemical properties.
摘要翻译： 本发明涉及地衣芽孢杆菌的25个迄今未描述的基因及其衍生的基因产物及其全部充分同源的核酸及其蛋白质。 它们发生在五种不同的代谢途径中，用于形成气味物质。 所讨论的代谢途径是用于合成：1）异戊酸（作为亮氨酸分解代谢的一部分），2）2-甲基丁酸和/或异丁酸（作为缬氨酸和/或异亮氨酸分解代谢的一部分） 3）丁醇和/或丁酸（作为丁酸代谢的一部分），4）丙酸（作为丙酸代谢的一部分）和/或5）尸胺和/或腐胺（作为赖氨酸分解代谢的一部分） 和/或精氨酸）。 这些基因的鉴定允许开发生物技术生产方法，其被改进到对于辅助这些核酸的程度，可以通过使微生物中相应的基因失活来减少通过这些代谢途径合成的恶臭物质的形成 用于生物技术生产。 此外，这些基因产物因此可用于制备反应或根据其各自生物化学性质的方法。
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公开(公告)号：US20170260548A1
公开(公告)日：2017-09-14
申请号：US15511676
申请日：2015-09-16
发明人： Philippe Marlière , Mathieu Allard
CPC分类号： C12P5/026 , C12N9/0079 , C12N9/1029 , C12N9/1217 , C12P5/02 , C12Y114/15004 , C12Y203/01019 , C12Y207/02007
摘要： Described is a method for the production of isobutene from 3-methylcrotonyl-CoA comprising the steps of:(a) enzymatically converting 3-methylcrotonyl-CoA into 3-methylbutyric acid; and (b) further enzymatically converting the thus produced 3-methylbutyric acid into isobutene. The conversion of 3-methylcrotonyl-CoA into 3-methylbutyric acid can be achieved by first enzymatically converting 3-methylcrotonyl-CoA into 3-methylbutyryl-CoA and further enzymatically converting the thus produced 3-methylbutyryl-CoA into 3-methylbutyric acid. Alternatively, the conversion of 3-methylcrotonyl-CoA into 3-methylbutyric acid can be achieved by first enzymatically converting 3-methylcrotonyl-CoA into 3-methylcrotonic acid and then further enzymatically converting the thus produced 3-methylcrotonic acid into 3-methylbutyric acid.
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