公开(公告)号：US20230125113A1

公开(公告)日：2023-04-27

申请号：US17821449

申请日：2022-08-22

Applicant: Becton, Dickinson and Company

Inventor： Christina Fan ,  Christina Chang ,  Eleen Shum ,  Olaf Zoellner ,  Nidhanjali Bansal ,  James Ghadiali ,  Gretchen Yinbon Lam ,  Eric Jensen ,  Jurg Rohrer ,  Brent Gaylord ,  Jody Martin ,  Jason G. Vidal

IPC: G01N33/566 ,  G16B99/00 ,  G01N33/50 ,  G01N33/543 ,  G01N33/68 ,  C12Q1/6804 ,  G16Z99/00 ,  C12N15/10 ,  C12Q1/6806 ,  C12Q1/6823 ,  C12Q1/6869 ,  C12Q1/68

Abstract: Some embodiments disclosed herein provide a plurality of compositions each comprising a protein binding reagent conjugated with an oligonucleotide. The oligonucleotide comprises a unique identifier for the protein binding reagent it is conjugated with, and the protein binding reagent is capable of specifically binding to a protein target. Further disclosed are methods and kits for quantitative analysis of a plurality of protein targets in a sample and for simultaneous quantitative analysis of protein and nucleic acid targets in a sample. Also disclosed herein are systems and methods for preparing a labeled biomolecule reagent, including a labeled biomolecule agent comprising a protein binding reagent conjugated with an oligonucleotide.

公开(公告)号：US20210198754A1

公开(公告)日：2021-07-01

申请号：US17192814

申请日：2021-03-04

Applicant: Becton, Dickinson and Company

Inventor： Christina Fan ,  Stephen P.A. Fodor ,  Glenn Fu ,  Geoffrey Richard Facer ,  Julie Wilhelmy

IPC: C12Q1/6888 ,  C12Q1/6874 ,  C12Q1/6876 ,  C12Q1/6881

Abstract: The disclosure provides for methods, compositions, and kits for multiplex nucleic acid analysis of single cells. The methods, compositions and systems may be used for massively parallel single cell sequencing. The methods, compositions and systems may be used to analyze thousands of cells concurrently. The thousands of cells may comprise a mixed population of cells (e.g., cells of different types or subtypes, different sizes).

公开(公告)号：US20200172957A1

公开(公告)日：2020-06-04

申请号：US16789311

申请日：2020-02-12

Applicant: BECTON, DICKINSON AND COMPANY

Inventor： Christina Chang ,  Christina Fan ,  Eleen Shum

IPC: C12Q1/6804 ,  C12Q1/6816 ,  C12Q1/6806 ,  C12Q1/6811 ,  C12Q1/6809 ,  C12Q1/6869 ,  C12Q1/6855 ,  C12Q1/6865 ,  C12Q1/6876 ,  G01N33/532

Abstract: Disclosed herein include systems, methods, compositions, and kits for sample identification. A sample indexing composition can comprise, for example, a protein binding reagent associated with a sample indexing oligonucleotide. Different sample indexing compositions can include sample indexing oligonucleotides with different sequences. Sample origin of cells can be identified based on the sequences of the sample indexing oligonucleotides. Sample indexing oligonucleotides can be barcoded using barcoded and lengthened using daisy-chaining primers.

公开(公告)号：US20190338357A1

公开(公告)日：2019-11-07

申请号：US16400885

申请日：2019-05-01

Applicant: Becton, Dickinson and Company

Inventor： Christina Fan ,  Elisabeth Marie Walczak

IPC: C12Q1/6876

Abstract: Disclosed herein include systems, methods, compositions, and kits for sample analysis. Nucleic acid fragments comprising a capture sequence (or a complement thereof) can be generated from double-stranded genomic deoxyribonucleic acid (gDNA), barcoded to generate single-stranded DNA (ssDNA) fragments, and sequenced. Information relating to the gDNA (e.g., genome, chromatin accessibility, methylome) can be determined based on the sequences of the ssDNA fragments in the sequencing data obtained.

公开(公告)号：US20230295742A1

公开(公告)日：2023-09-21

申请号：US18194590

申请日：2023-03-31

Applicant: Becton, Dickinson and Company

Inventor： Christina Fan ,  Stephen P.A. Fodor ,  Glenn Fu ,  Geoffrey Richard Facer ,  Julie Wilhelmy

IPC: C12Q1/6888 ,  C12Q1/6874 ,  C12Q1/6876 ,  C12Q1/6881

CPC classification number: C12Q1/6888 ,  C12Q1/6874 ,  C12Q1/6876 ,  C12Q1/6881 ,  C12Q2600/158 ,  C12Q2600/16

Abstract: The disclosure provides for methods, compositions, and kits for multiplex nucleic acid analysis of single cells. The methods, compositions and systems may be used for massively parallel single cell sequencing. The methods, compositions and systems may be used to analyze thousands of cells concurrently. The thousands of cells may comprise a mixed population of cells (e.g., cells of different types or subtypes, different sizes).

公开(公告)号：US20230295723A1

公开(公告)日：2023-09-21

申请号：US18324880

申请日：2023-05-26

Applicant: Becton, Dickinson and Company

Inventor： Christina Fan ,  Elisabeth Marie Walczak

IPC: C12Q1/6876

CPC classification number: C12Q1/6876

Abstract: Disclosed herein include systems, methods, compositions, and kits for sample analysis. Nucleic acid fragments comprising a capture sequence (or a complement thereof) can be generated from double-stranded genomic deoxyribonucleic acid (gDNA), barcoded to generate single-stranded DNA (ssDNA) fragments, and sequenced. Information relating to the gDNA (e.g., genome, chromatin accessibility, methylome) can be determined based on the sequences of the ssDNA fragments in the sequencing data obtained.

公开(公告)号：US11365409B2

公开(公告)日：2022-06-21

申请号：US16400886

申请日：2019-05-01

Applicant: Becton, Dickinson and Company

Inventor： Eleen Shum ,  Christina Fan

IPC: C12N15/10 ,  C12Q1/6816

Abstract: Disclosed herein include systems, methods, compositions, and kits for molecular barcoding on the 5′-end of a nucleic acid target. After barcoding a nucleic acid target using an oligonucleotide barcode comprising a target binding region and a molecular label to generate a barcoded nucleic acid molecule, an oligonucleotide comprising a complement of the target binding region can be added to generate a barcoded nucleic acid molecule comprising the target-binding region and the complement of the target-binding region. A stem loop is formed with intra-molecular hybridization of the barcoded nucleic acid molecule, which can be extended to generate an extended barcoded nucleic acid molecule comprising the molecular label and a complement of the molecular label.

公开(公告)号：US10253375B1

公开(公告)日：2019-04-09

申请号：US16162161

申请日：2018-10-16

Applicant: Becton, Dickinson and Company

Inventor： Christina Fan ,  Stephen P. A. Fodor ,  Glenn Fu ,  Geoffrey Richard Facer ,  Julie Wilhelmy

IPC: C12Q1/68 ,  C12Q1/6888 ,  C12Q1/6876 ,  C12Q1/6881 ,  C12Q1/6874

Abstract: The disclosure provides for methods, compositions, and kits for multiplex nucleic acid analysis of single cells. The methods, compositions and systems may be used for massively parallel single cell sequencing. The methods, compositions and systems may be used to analyze thousands of cells concurrently. The thousands of cells may comprise a mixed population of cells (e.g., cells of different types or subtypes, different sizes).

公开(公告)号：US20190085412A1

公开(公告)日：2019-03-21

申请号：US16162161

申请日：2018-10-16

Applicant: Becton, Dickinson and Company

Inventor： Christina Fan ,  Stephen P.A. Fodor ,  Glenn Fu ,  Geoffrey Richard Facer ,  Julie Wilhelmy

IPC: C12Q1/6888 ,  C12Q1/6876 ,  C12Q1/6881 ,  C12Q1/6874

Abstract: The disclosure provides for methods, compositions, and kits for multiplex nucleic acid analysis of single cells. The methods, compositions and systems may be used for massively parallel single cell sequencing. The methods, compositions and systems may be used to analyze thousands of cells concurrently. The thousands of cells may comprise a mixed population of cells (e.g., cells of different types or subtypes, different sizes).

公开(公告)号：US20190040474A1

公开(公告)日：2019-02-07

申请号：US16161981

申请日：2018-10-16

Applicant: Becton, Dickinson and Company

Inventor： Christina Fan ,  Stephen P.A. Fodor ,  Glenn Fu ,  Geoffrey Richard Facer ,  Julie Wilhelmy

IPC: C12Q1/6888 ,  C12Q1/6874 ,  C12Q1/6876 ,  C12Q1/6881

Abstract: The disclosure provides for methods, compositions, and kits for multiplex nucleic acid analysis of single cells. The methods, compositions and systems may be used for massively parallel single cell sequencing. The methods, compositions and systems may be used to analyze thousands of cells concurrently. The thousands of cells may comprise a mixed population of cells (e.g., cells of different types or subtypes, different sizes).