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公开(公告)号：US20080213843A1
公开(公告)日：2008-09-04
申请号：US12034906
申请日：2008-02-21
申请人： Bjarne Ronfeldt Nielsen , Allan Svendsen , Henrik Pedersen , Jesper Vind , Hanne Vang Hendriksen , Torben Peter Frandsen
发明人： Bjarne Ronfeldt Nielsen , Allan Svendsen , Henrik Pedersen , Jesper Vind , Hanne Vang Hendriksen , Torben Peter Frandsen
CPC分类号： C12N9/2428 , C12P19/20 , Y02E50/17
摘要： The invention relates to a variant of a parent fungal glucoamylase, which exhibits altered properties, in particular improved thermal stability and/or increased specific activity.
摘要翻译： 本发明涉及亲本真菌葡糖淀粉酶的变体，其具有改变的性质，特别是改进的热稳定性和/或增加的比活性。
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公开(公告)号：US07927857B2
公开(公告)日：2011-04-19
申请号：US12034906
申请日：2008-02-21
申请人： Bjarne Ronfeldt Nielsen , Allan Svendsen , Henrik Pedersen , Jesper Vind , Hanne Vang Hendriksen , Torben Peter Frandsen
发明人： Bjarne Ronfeldt Nielsen , Allan Svendsen , Henrik Pedersen , Jesper Vind , Hanne Vang Hendriksen , Torben Peter Frandsen
CPC分类号： C12N9/2428 , C12P19/20 , Y02E50/17
摘要： The invention relates to a variant of a parent fungal glucoamylase, which exhibits altered properties, in particular improved thermal stability and/or increased specific activity.
摘要翻译： 本发明涉及亲本真菌葡糖淀粉酶的变体，其具有改变的性质，特别是改进的热稳定性和/或增加的比活性。
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公开(公告)号：US20100098804A1
公开(公告)日：2010-04-22
申请号：US12643104
申请日：2009-12-21
申请人： Joel Robert Cherry , Allan Svendsen , Carsten Andersen , Lars Beier , Torben Peter Frandsen , Thomas Schäfer
发明人： Joel Robert Cherry , Allan Svendsen , Carsten Andersen , Lars Beier , Torben Peter Frandsen , Thomas Schäfer
CPC分类号： C12N9/2417 , A21D8/042
摘要： The inventors have discovered some striking, and not previously predicted structural similarities and differences between the structure of Novamyl and the reported structures of CGTases, and based on this they have constructed variants of maltogenic alpha-amylase having CGTase activity and variants of CGTase having maltogenic alpha-amylase activity. Further, on the basis of sequence homology between Novamyl® and CGTases, the inventors have constructed hybrid enzymes with one or more improvements to specific properties of the parent enzymes, using recombinant DNA methodology.
摘要翻译： 发明人已经发现了一些引人注目的，并没有预先结合的Novamyl结构与报道的CGTase结构之间的结构相似性和差异，并且基于此，它们构建了具有CGT酶活性的麦芽糖α-淀粉酶变异体和具有麦芽糖α 淀粉酶活性。 此外，基于Novamyl和CGTases之间的序列同源性，发明人使用重组DNA方法构建了对母体酶的特定性质的一个或多个改进的杂合酶。
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公开(公告)号：US20090142803A1
公开(公告)日：2009-06-04
申请号：US12208425
申请日：2008-09-11
申请人： Joel Robert Cherry , Allan Svendsen , Carsten Denmark Andersen , Lars Beier , Torben Peter Frandsen , Thomas Schafer
发明人： Joel Robert Cherry , Allan Svendsen , Carsten Denmark Andersen , Lars Beier , Torben Peter Frandsen , Thomas Schafer
CPC分类号： C12N9/2417 , A21D8/042
摘要： The inventors have discovered some striking, and not previously predicted structural similarities and differences between the structure of Novamyl and the reported structures of CGTases, and based on this they have constructed variants of maltogenic alpha-amylase having CGTase activity and variants of CGTase having maltogenic alpha-amylase activity. Further, on the basis of sequence homology between Novamyl® and CGTases, the inventors have constructed hybrid enzymes with one or more improvements to specific properties of the parent enzymes, using recombinant DNA methodology.
摘要翻译： 发明人已经发现了一些引人注目的，并没有预先结合的Novamyl结构与报道的CGTase结构之间的结构相似性和差异，并且基于此，它们构建了具有CGT酶活性的麦芽糖α-淀粉酶变异体和具有麦芽糖α 淀粉酶活性。 此外，基于Novamyl和CGTases之间的序列同源性，本发明人使用重组DNA方法构建了对母体酶的特定性质具有一个或多个改进的杂合酶。
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公开(公告)号：US20110159570A1
公开(公告)日：2011-06-30
申请号：US13010219
申请日：2011-01-20
IPC分类号： C12N9/28
CPC分类号： C12N9/2417 , A21D2/26 , A21D8/042
摘要： The inventors have modified the amino acid sequence of a maltogenic alpha-amylase to obtain variants with improved properties, based on the three-dimensional structure of the maltogenic alpha-amylase Novamyl. The variants have altered physicochemical properties, e.g. an altered pH optimum, improved thermostability, increased specific activity, an altered cleavage pattern or an increased ability to reduce retrogradation of starch or staling of bread.
摘要翻译： 基于麦芽糖α-淀粉酶Novamyl的三维结构，本发明人已经修饰了产麦芽糖α-淀粉酶的氨基酸序列以获得具有改进性质的变体。 这些变体具有改变的物理化学性质，例如 改变的pH最佳，改善的热稳定性，增加的比活性，改变的切割模式或增加降低淀粉退化或面包陈化的能力。
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16.发明授权Processes for preparing a dough or a baked product using maltogenic alpha-amylase variants 有权使用产麦芽糖α-淀粉酶变体制备面团或烘焙产品的方法公开(公告)号：US08546099B2
公开(公告)日：2013-10-01
申请号：US13533055
申请日：2012-06-26
CPC分类号： C12N9/2417 , A21D2/26 , A21D8/042
摘要： The inventors have modified the amino acid sequence of a maltogenic alpha-amylase to obtain variants with improved properties, based on the three-dimensional structure of the maltogenic alpha-amylase Novamyl. The variants have altered physicochemical properties, e.g. an altered pH optimum, improved thermostability, increased specific activity, an altered cleavage pattern or an increased ability to reduce retrogradation of starch or staling of bread.
摘要翻译： 基于麦芽糖α-淀粉酶Novamyl的三维结构，本发明人已经修饰了产麦芽糖α-淀粉酶的氨基酸序列以获得具有改进性质的变体。 这些变体具有改变的物理化学性质，例如 改变的pH最佳，改善的热稳定性，增加的比活性，改变的切割模式或增加降低淀粉退化或面包陈化的能力。
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公开(公告)号：US07908094B2
公开(公告)日：2011-03-15
申请号：US12413758
申请日：2009-03-30
CPC分类号： C12N9/2417 , A21D2/26 , A21D8/042
摘要： The inventors have modified the amino acid sequence of a maltogenic alpha-amylase to obtain variants with improved properties, based on the three-dimensional structure of the maltogenic alpha-amylase Novamyl. The variants have altered physicochemical properties, e.g. an altered pH optimum, improved thermostability, increased specific activity, an altered cleavage pattern or an increased ability to reduce retrogradation of starch or staling of bread.
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公开(公告)号：US6162628A
公开(公告)日：2000-12-19
申请号：US386607
申请日：1999-08-31
CPC分类号： C12N9/2417 , A21D8/042 , C07K2299/00
摘要： The inventors have modified the amino acid sequence of a maltogenic alpha-amylase to obtain variants with improved properties, based on the three-dimensional structure of the maltogenic alpha-amylase Novamyl. The variants have altered physicochemical properties, e.g. an altered pH optimum, improved thermostability, increased specific activity, an altered cleavage pattern or an increased ability to reduce retrogradation of starch or staling of bread.
摘要翻译： 基于麦芽糖α-淀粉酶Novamyl的三维结构，本发明人已经修饰了产麦芽糖α-淀粉酶的氨基酸序列以获得具有改进性质的变体。 这些变体具有改变的物理化学性质，例如 改变的pH最佳，改善的热稳定性，增加的比活性，改变的切割模式或增加降低淀粉退化或面包陈化的能力。
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公开(公告)号：US20090203108A1
公开(公告)日：2009-08-13
申请号：US12413758
申请日：2009-03-30
IPC分类号： C12N9/28
CPC分类号： C12N9/2417 , A21D2/26 , A21D8/042
摘要： The inventors have modified the amino acid sequence of a maltogenic alpha-amylase to obtain variants with improved properties, based on the three-dimensional structure of the maltogenic alpha-amylase Novamyl. The variants have altered physicochemical properties., e.g. an altered pH optimum, improved thermostability, increased specific activity, an altered cleavage pattern or an increased ability to reduce retrogradation of starch or staling of bread.
摘要翻译： 基于麦芽糖α-淀粉酶Novamyl的三维结构，本发明人已经修饰了产麦芽糖α-淀粉酶的氨基酸序列以获得具有改进性质的变体。 这些变体具有改变的物理化学性质。 改变的pH最佳，改善的热稳定性，增加的比活性，改变的切割模式或增加降低淀粉退化或面包陈化的能力。
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公开(公告)号：US20120270253A1
公开(公告)日：2012-10-25
申请号：US13533055
申请日：2012-06-26
CPC分类号： C12N9/2417 , A21D2/26 , A21D8/042
摘要： The inventors have modified the amino acid sequence of a maltogenic alpha-amylase to obtain variants with improved properties, based on the three-dimensional structure of the maltogenic alpha-amylase Novamyl. The variants have altered physicochemical properties., e.g. an altered pH optimum, improved thermostability, increased specific activity, an altered cleavage pattern or an increased ability to reduce retrogradation of starch or staling of bread.
摘要翻译： 基于麦芽糖α-淀粉酶Novamyl的三维结构，本发明人已经修饰了产麦芽糖α-淀粉酶的氨基酸序列以获得具有改进性质的变体。 这些变体具有改变的物理化学性质。 改变的pH最佳，改善的热稳定性，增加的比活性，改变的切割模式或增加降低淀粉退化或面包陈化的能力。
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