公开(公告)号：US20150190806A1

公开(公告)日：2015-07-09

申请号：US14418215

申请日：2013-07-18

Applicant: Corning Incorporated

Inventor： Po Ki Yuen

IPC: B01L3/00

CPC classification number: B01L3/502746 ,  B01L3/5023 ,  B01L2200/0621 ,  B01L2200/0642 ,  B01L2200/0678 ,  B01L2300/069 ,  B01L2400/0406 ,  B01L2400/0466 ,  B01L2400/084

Abstract: A method of operating a microfluidic device (15), wherein the microfluidic device comprises a microfluidic channel (20), a fluid conveyance extension (30), and an absorbent microfluidic flow modulator (35). The microfluidic channel extends from a channel outlet chamber (25) of the microfluidic device and the fluid conveyance extension is fluidly coupled to the channel outlet chamber. The absorbent microfluidic flow modulator is configured to absorb a fluid from the fluid conveyance extension when fluidly coupled to the fluid conveyance extension. The method comprises admitting the fluid into the microfluidic channel and the channel outlet chamber, saturating the fluid conveyance extension with the fluid, and generating a fluid flow in the microfluidic channel by fluidly coupling the absorbent microfluidic flow modulator to the fluid conveyance extension to absorb the fluid from the fluid conveyance extension.

Abstract translation: 一种操作微流体装置（15）的方法，其中所述微流体装置包括微流体通道（20），流体输送延伸部（30）和吸收性微流体流动调节器（35）。 微流体通道从微流体装置的通道出口室（25）延伸，并且流体输送延伸部流体地联接到通道出口室。 吸收性微流体流动调节器构造成当流体连接到流体输送延伸部时从流体输送延伸部吸收流体。 该方法包括将流体进入微流体通道和通道出口室，使流体输送延伸部饱和，并且通过将吸收性微流体流动调节器流体耦合到流体输送延伸部以吸收流体流动，从而在微流体通道中产生流体流 来自流体输送延伸部的流体。

公开(公告)号：US11136542B2

公开(公告)日：2021-10-05

申请号：US16077573

申请日：2017-02-13

Applicant: Corning Incorporated

Inventor： Sumitava De ,  Yulong Hong ,  Nikolaos Pantelis Kladias ,  Shawn Michael O'Malley ,  Aravind Raghavan Rammohan ,  Po Ki Yuen

IPC: C12N1/00 ,  C12M1/04 ,  C12M1/00 ,  C12M1/06 ,  C12M1/12

Abstract: A perfusion bioreactor and a method for using the perfusion bioreactor for performing a continuous cell culture are disclosed. The perfusion bioreactor includes an outer vessel having a housing with a gas permeable membrane, an opening and a cavity; an inner vessel within the cavity; and a lid to cover the opening. A porous membrane within the cavity divides the cavity into inner and outer compartments. A fresh media port extends through the outer vessel or the at least one lid to receive a fresh media tube that has an end located in the inner compartment. A spent media port extends through the outer vessel or the at least one lid to receive a spent media tube that has an end located in the outer compartment. A mixer is within the inner compartment, and the porous membrane is attached to an opening within the inner vessel.

公开(公告)号：US20150004686A1

公开(公告)日：2015-01-01

申请号：US14375032

申请日：2013-01-31

Applicant: CORNING INCORPORATED

Inventor： Vasiliy Nikolaevich Goral ,  Fang Lai ,  Po Ki Yuen

IPC: C12M1/00

Abstract: A microplate for culturing cells with automatic, continuous perfusion includes a well frame and a planar substrate. The well frame and the planar substrate form a first well, a second well, and a third well. The first well is fluidly connected with the second well with a first perfusion membrane and the first well is fluidly connected with the third well with a second perfusion membrane. Methods of fabricating the microplate and methods of culturing cells are also provided.

Abstract translation: 用于用自动连续灌注培养细胞的微板包括井架和平面基底。 井架和平面底板形成第一井，第二井和第三井。 第一个井用第一个灌注膜与第二个井流体连接，第一个井用第二个灌注膜与第三个井流体连接。 还提供了制备微孔板的方法和培养细胞的方法。

公开(公告)号：US08679843B2

公开(公告)日：2014-03-25

申请号：US13911490

申请日：2013-06-06

Applicant: Corning Incorporated

Inventor： Ronald Allen Faris ,  Vasiliy Nikolaevich Goral ,  Miya Yi-Cheng Hsieh ,  Odessa Natalie Petzold ,  Po Ki Yuen

IPC: C12N5/071

CPC classification number: G01N33/5008 ,  C12M21/08 ,  C12M23/16 ,  C12M23/24 ,  C12M23/40 ,  C12M29/10

Abstract: A microfluidic cell culture apparatus includes a cell retention chamber and a perfusion channel. The cell retention chamber has a structured surface. The structured surface includes a major surface from which a plurality of projections extends into the chamber. The plurality of projections are arranged to suspend cells cultured in the chamber above the major surface. The first perfusion channel is configured to provide laminar flow of a fluid through the channel and forms a plurality of openings in communication with the cell retention chamber. The openings are configured to prevent cells from the retention chamber from entering the perfusion channel.

Abstract translation: 微流体细胞培养装置包括细胞保留室和灌注通道。 细胞保留室具有结构化表面。 结构化表面包括主表面，多个突起从该主表面延伸到腔室中。 多个突起被布置成悬浮在主表面上方的室中培养的细胞。 第一灌注通道构造成提供流体通过通道的层流，并形成与细胞保持室连通的多个开口。 开口构造成防止来自保留腔的细胞进入灌注通道。

公开(公告)号：US20130273589A1

公开(公告)日：2013-10-17

申请号：US13911490

申请日：2013-06-06

Applicant: Corning Incorporated

Inventor： Ronald Allen Faris ,  Vasiliy Nikolaevich Goral ,  Miya Yi-Cheng Hsieh ,  Odessa Natalie Petzold ,  Po Ki Yuen

IPC: G01N33/50

CPC classification number: G01N33/5008 ,  C12M21/08 ,  C12M23/16 ,  C12M23/24 ,  C12M23/40 ,  C12M29/10

Abstract: A microfluidic cell culture apparatus includes a cell retention chamber and a perfusion channel. The cell retention chamber has a structured surface. The structured surface includes a major surface from which a plurality of projections extends into the chamber. The plurality of projections are arranged to suspend cells cultured in the chamber above the major surface. The first perfusion channel is configured to provide laminar flow of a fluid through the channel and forms a plurality of openings in communication with the cell retention chamber. The openings are configured to prevent cells from the retention chamber from entering the perfusion channel.

Abstract translation: 微流体细胞培养装置包括细胞保留室和灌注通道。 细胞保留室具有结构化表面。 结构化表面包括主表面，多个突起从该主表面延伸到腔室中。 多个突起被布置成悬浮在主表面上方的室中培养的细胞。 第一灌注通道构造成提供流体通过通道的层流，并形成与细胞保持室连通的多个开口。 开口构造成防止来自保留腔的细胞进入灌注通道。