公开(公告)号：US20240125783A1

公开(公告)日：2024-04-18

申请号：US18491255

申请日：2023-10-20

Applicant: California Institute of Technology

Inventor： Mitchell Guttman ,  Mario R. Blanco ,  Ward Walkup, IV

IPC: G01N33/573 ,  C12N15/90

CPC classification number: G01N33/573 ,  C12N15/907 ,  C12Y201/01043

Abstract: In some embodiments, methods of detecting an association between a query protein and a target moiety are described. In some embodiments, compositions are described. In some embodiments, kits are described.

公开(公告)号：US20230408504A1

公开(公告)日：2023-12-21

申请号：US18167362

申请日：2023-02-10

Applicant: California Institute of Technology

Inventor： Mitchell Guttman ,  Andrew A. Perez ,  Mario R. Blanco ,  Isabel N. Goronzy ,  James K. Guo

IPC: G01N33/543

CPC classification number: G01N33/54353 ,  G01N33/54326 ,  G01N33/54346

Abstract: Disclosed herein include methods, compositions, and kits suitable for use in generating barcoded detection particles. Each barcoded detection particle can comprise a particle associated with an antigen-binding protein and a plurality of barcoding oligonucleotides. The plurality of barcoding oligonucleotides can comprise a first ligand. The particle can comprise a second ligand. The plurality of barcoding oligonucleotides can be associated with the particle via a multivalent binding agent comprising two or more binding moieties capable of binding the first ligand and/or the second ligand. There are provided, in some embodiments, methods for detecting interactions between nucleic acid molecules and proteins of interest. Methods for detecting interactions between ribonucleic acid molecules and RNA-binding proteins (RBPs) are also provided herein.

公开(公告)号：US11828684B2

公开(公告)日：2023-11-28

申请号：US16573673

申请日：2019-09-17

Applicant: California Institute of Technology

Inventor： Mitchell Guttman ,  Mario R. Blanco ,  Ward Walkup, IV

IPC: G01N33/573 ,  C12N15/90

CPC classification number: G01N33/573 ,  C12N15/907 ,  C12Y201/01043

Abstract: In some embodiments, methods of detecting an association between a query protein and a target moiety are described. In some embodiments, compositions are described. In some embodiments, kits are described.

公开(公告)号：US20170044609A1

公开(公告)日：2017-02-16

申请号：US15336748

申请日：2016-10-27

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Mitchell Guttman ,  Chun-Kan Chen ,  Colleen A. McHugh

IPC: C12Q1/68 ,  G01N33/50 ,  G01N33/68

CPC classification number: C12Q1/6876 ,  C12Q2600/136 ,  C12Q2600/158 ,  G01N33/5008 ,  G01N33/5023 ,  G01N33/6848

Abstract: Methods of prohibiting Xist-dependent silencing of X chromosome genes include targeting the required Xist silencing complex components including SHARP, SMRT, HDAC3, SAF-A, LBR, and the respective binding sites of SHARP, LBR, and SAF-A on Xist, thereby prohibiting Xist repression, allowing for reactivation of the silenced X chromosome genes.

Abstract translation: 禁止X染色体基因Xist依赖性沉默的方法包括靶向所需的Xist沉默复合物成分，包括SHARP，SMRT，HDAC3，SAF-A，LBR以及Xist上SHARP，LBR和SAF-A的各自的结合位点，从而 禁止Xist镇压，允许重新激活沉默的X染色体基因。