公开(公告)号：US07476498B2

公开(公告)日：2009-01-13

申请号：US11192070

申请日：2005-07-29

申请人： Manfred Motz ,  Georg Bauer ,  Erwin Soutschek

发明人： Manfred Motz ,  Georg Bauer ,  Erwin Soutschek

IPC分类号： C12Q1/70

CPC分类号： G01N33/56994

摘要： In the area of virus diagnosis, in particular Epstein-Barr virus (EBV) diagnosis, methods for the detection of EBV and agents suitable for this purpose are provided, including peptides which are derived from p18-VCA and permit discrimination between acute and past EBV infection.

摘要翻译： 在病毒诊断领域，特别是EB病毒（EBV）诊断中，提供了用于检测EBV的方法和适合于此目的的试剂，包括衍生自p18-VCA的肽，并允许区分急性和过去的EBV 感染。

公开(公告)号：US06808711B2

公开(公告)日：2004-10-26

申请号：US10403220

申请日：2003-03-26

申请人： Manfred Motz ,  Erwin Soutschek

发明人： Manfred Motz ,  Erwin Soutschek

IPC分类号： A61K3902

CPC分类号： C07K14/20 ,  A61K39/00 ,  A61K2039/51 ,  Y02A50/401

摘要： The present invention relates to immunologically active proteins from Borrelia burgdorferi which are present in a form which is free of other proteins derived from Borrelia burgdorferi and which exhibit the sequence of the protein 1829-22A, which has the amino acid sequence (SEQ. ID NO:1) MKKFNLIIEALFAILLTACNFGLMEETKIALESSSKDVKNKILQIKKDAE DKGVNFAAFTSSETGSKVTNGGLALREAKIQAINEVEKFLKRIEEEALKL KEHGNSGQFLELFDLLLEVLESLEPIGIKGLKDFISEEAKCNPISTSER LIEVKVQIENKMEEVKRKQNLNKERKSNKGKKKK or a part sequence therefor having at least 10 consecutive amino acids, or exhibit the sequence of the protein 1829-22B, which has the amino acid sequence (SEQ. ID NO:2) MIKYNKIILTLTLLASLLAACSLTGKARLESSVKDITNEIEKAIKEAEDA GVKTDAFTETQTGGKVAGPKIRAAKIRVADLTIKFLEATEEETITFKENG AGEDEFSGIYDLILNAAKAVEKIGMKDMTKTVEEAAKENPKTTANGIIEI VKVMKAKVENIKEKQTKNQK or part sequence thereof having at least 10 consecutive amino acids.

公开(公告)号：US10669350B2

公开(公告)日：2020-06-02

申请号：US15543215

申请日：2016-02-01

申请人： Georg Bauer ,  Manfred Motz

发明人： Georg Bauer ,  Manfred Motz

IPC分类号： C07K16/40 ,  C07K16/30 ,  A61K31/337 ,  A61K39/395 ,  A61K45/06 ,  A61K39/00

摘要： The invention relates to single domain VHH fragments which specifically bind to and inhibit superoxide dismutase and/or bind to and inhibit catalase and/or bind to and inhibit superoxide dismutase and catalase, in particular for the use in the therapy of tumor diseases.

公开(公告)号：US06183755B2

公开(公告)日：2001-02-06

申请号：US09196293

申请日：1998-11-19

申请人： Manfred Motz ,  Erwin Soutscheck ,  Renate Fuchs ,  Bettina Wilske ,  Vera Preac-Mursic

发明人： Manfred Motz ,  Erwin Soutscheck ,  Renate Fuchs ,  Bettina Wilske ,  Vera Preac-Mursic

IPC分类号： A61K3902

CPC分类号： C07K16/1207 ,  A61K38/00 ,  A61K39/00 ,  C07K14/20 ,  G01N33/56911 ,  Y02A50/401 ,  Y02A50/57 ,  Y10S436/804 ,  Y10S530/825

摘要： Various immunologically active proteins from Borrelia burgdorferi have been prepared by genetic manipulation in microorganisms. To do this, the specific DNA sequences were selected from a B. burgdorferi gene bank using suitable screening methods, or were prepared directly by DNA amplification using selected hybridization probes, and were placed under the control of inducible promoters such as, for example, the lac promoter. It has been possible, owing to description of efficient purification methods for the expressed antigens, to provide the proteins in a suitable way. These proteins can be used to produce specific and sensitive diagnostic assay kits. The specific combination of the immunologically active proteins makes precise diagnosis possible. Furthermore, monoclonal antibodies have been generated and are used as reagents for detecting pathogens directly in test samples or after cultivation. The Borrelia burgdorferi-specific DNA sequences can be employed for direct detection of the pathogen in patients' samples (for example by means of the PCR reaction).

摘要翻译： 通过遗传操作在微生物中制备了来自博氏疏螺旋体的各种免疫活性蛋白质。 为此，使用合适的筛选方法从布氏疏螺旋体基因库中选择特异性DNA序列，或者通过使用选择的杂交探针的DNA扩增直接制备，并置于诱导型启动子的控制下，例如， lac启动子。 由于对表达的抗原的有效纯化方法的描述，可以以合适的方式提供蛋白质是可能的。 这些蛋白质可用于制备特异性敏感的诊断测定试剂盒。 免疫活性蛋白质的特异性组合使得精确的诊断成为可能。 此外，已经产生了单克隆抗体，并且用作用于在测试样品中或培养后直接检测病原体的试剂。 疏螺旋体异常特异性DNA序列可用于直接检测患者样品中的病原体（例如通过PCR反应）。

公开(公告)号：US06509019B1

公开(公告)日：2003-01-21

申请号：US09711546

申请日：2000-11-13

申请人： Renate Fuchs ,  Bettina Wilske ,  Vera Preac-Mursic ,  Manfred Motz ,  Erwin Soutscheck

发明人： Renate Fuchs ,  Bettina Wilske ,  Vera Preac-Mursic ,  Manfred Motz ,  Erwin Soutscheck

IPC分类号： A61K3902

CPC分类号： C07K16/1207 ,  A61K38/00 ,  A61K39/00 ,  C07K14/20 ,  G01N33/56911 ,  Y02A50/401 ,  Y02A50/57 ,  Y10S436/804 ,  Y10S530/825

摘要： Various immunologically active proteins from Borrelia burgdorferi have been prepared by genetic manipulation in microorganisms. To do this, the specific DNA sequences were selected from a B. burgdorferi gene bank using suitable screening methods, or were prepared directly by DNA amplification using selected hybridization probes, and were placed under the control of inducible promoters such as, for example, the lac promoter. It has been possible, owing to description of efficient purification methods for the expressed antigens, to provide the proteins in a suitable way. These proteins can be used to produce specific and sensitive diagnostic assay kits. The specific combination of the immunologically active proteins makes precise diagnosis possible. Furthermore, monoclonal antibodies have been generated and are used as reagents for detecting pathogens directly in test samples or after cultivation. The Borrelia burgdorferi-specific DNA sequences can be employed for direct detection of the pathogen in patients' samples (for example by means of the PCR reaction).

摘要翻译： 通过遗传操作在微生物中制备了来自博氏疏螺旋体的各种免疫活性蛋白质。 为此，使用合适的筛选方法从布氏疏螺旋体基因库中选择特异性DNA序列，或者通过使用选择的杂交探针的DNA扩增直接制备，并置于诱导型启动子的控制下，例如， lac启动子。 由于对表达的抗原的有效纯化方法的描述，可以以合适的方式提供蛋白质是可能的。 这些蛋白质可用于制备特异性敏感的诊断测定试剂盒。 免疫活性蛋白质的特异性组合使得精确的诊断成为可能。 此外，已经产生了单克隆抗体，并且用作用于在测试样品中或培养后直接检测病原体的试剂。 疏螺旋体异常特异性DNA序列可用于直接检测患者样品中的病原体（例如通过PCR反应）。

公开(公告)号：US07083792B2

公开(公告)日：2006-08-01

申请号：US10762665

申请日：2004-01-22

申请人： Renate Fuchs ,  Bettina Wilske ,  Vera Preac-Mursic ,  Manfred Motz ,  Erwin Soutscheck

发明人： Renate Fuchs ,  Bettina Wilske ,  Vera Preac-Mursic ,  Manfred Motz ,  Erwin Soutscheck

IPC分类号： A61K39/00 ,  A61K39/02 ,  C07K1/00 ,  C07K14/00

CPC分类号： C07K16/1207 ,  A61K38/00 ,  A61K39/00 ,  C07K14/20 ,  G01N33/56911 ,  Y02A50/401 ,  Y02A50/57 ,  Y10S436/804 ,  Y10S530/825

摘要： Various immunologically active proteins from Borrelia burgdorferi have been prepared by genetic manipulation in microorganisms. To do this, the specific DNA sequences were selected from a B. burgdorferi gene bank using suitable screening methods, or were prepared directly by DNA amplification using selected hybridization probes, and were placed under the control of inducible promoters such as, for example, the lac promoter. It has been possible, owing to description of efficient purification methods for the expressed antigens, to provide the proteins in a suitable way. These proteins can be used to produce specific and sensitive diagnostic assay kits. The specific combination of the immunologically active proteins makes precise diagnosis possible. Furthermore, monoclonal antibodies have been generated and are used as reagents for detecting pathogens directly in test samples or after cultivation. The Borrelia burgdorferi-specific DNA sequences can be employed for direct detection of the pathogen in patients' samples (for example by means of the PCR reaction).

公开(公告)号：US5741656A

公开(公告)日：1998-04-21

申请号：US586640

申请日：1996-04-29

申请人： Hans Joachim Wolf ,  Udo Reischl ,  Manfred Motz

发明人： Hans Joachim Wolf ,  Udo Reischl ,  Manfred Motz

IPC分类号： C12N15/09 ,  A61K38/00 ,  A61K39/00 ,  A61K39/245 ,  A61P31/12 ,  C07K14/05 ,  C07K16/08 ,  C12N1/21 ,  C12N15/38 ,  C12P21/02 ,  C12Q1/68 ,  C12Q1/70 ,  C12R1/19 ,  C12R1/92 ,  G01N33/53 ,  C12P19/34 ,  C12P21/00 ,  G01N33/48

CPC分类号： C12Q1/705 ,  C07K14/005 ,  A61K38/00 ,  A61K39/00 ,  C07K2319/00 ,  C12N2710/16222

摘要： The application describes the identification of Epstein-Barr virus (EBV) encoded gene and rapid methods for construction of plasmids efficiently expressing this or other genes. The EBV gene can be purified and used as an antigen for diagnosis and therapy of EBV related diseases.

摘要翻译： PCT No.PCT / EP94 / 02436 Sec。 371日期：1996年4月29日 102（e）日期1996年4月29日PCT 1994年7月22日PCT公布。 公开号WO95 / 03415 日期1995年2月2日该应用描述了爱泼斯坦 - 巴尔病毒（EBV）编码基因的鉴定和用于构建有效表达该基因或其他基因的质粒的快速方法。 可以纯化EBV基因并将其用作EBV相关疾病的诊断和治疗的抗原。

公开(公告)号：US06753183B2

公开(公告)日：2004-06-22

申请号：US10289795

申请日：2002-11-07

申请人： Renate Fuchs ,  Bettina Wilske ,  Vera Preac-Mursic ,  Manfred Motz ,  Erwin Soutscheck

发明人： Renate Fuchs ,  Bettina Wilske ,  Vera Preac-Mursic ,  Manfred Motz ,  Erwin Soutscheck

IPC分类号： G01N3353

CPC分类号： C07K16/1207 ,  A61K38/00 ,  A61K39/00 ,  C07K14/20 ,  G01N33/56911 ,  Y02A50/401 ,  Y02A50/57 ,  Y10S436/804 ,  Y10S530/825

摘要： Various immunologically active proteins from Borrelia burgdorferi have been prepared by genetic manipulation in microorganisms. To do this, the specific DNA sequences were selected from a B. burgdorferi gene bank using suitable screening methods, or were prepared directly by DNA amplification using selected hybridization probes, and were placed under the control of inducible promoters such as, for example, the lac promoter. It has been possible, owing to description of efficient purification methods for the expressed antigens, to provide the proteins in a suitable way. These proteins can be used to produce specific and sensitive diagnostic assay kits. The specific combination of the immunologically active proteins makes precise diagnosis possible. Furthermore, monoclonal antibodies have been generated and are used as reagents for detecting pathogens directly in test samples or after cultivation. The Borrelia burgdorferi—specific DNA sequences can be employed for direct detection of the pathogen in patients' samples (for example by means of the PCR reaction).

摘要翻译： 通过遗传操作在微生物中制备了来自博氏疏螺旋体的各种免疫活性蛋白质。 为此，使用合适的筛选方法从布氏疏螺旋体基因库中选择特异性DNA序列，或者通过使用选择的杂交探针的DNA扩增直接制备，并置于诱导型启动子的控制下，例如， lac启动子。 由于对表达的抗原的有效纯化方法的描述，可以以合适的方式提供蛋白质是可能的。 这些蛋白质可用于制备特异性敏感的诊断测定试剂盒。 免疫活性蛋白质的特异性组合使得精确的诊断成为可能。 此外，已经产生了单克隆抗体，并且用作用于在测试样品中或培养后直接检测病原体的试剂。 疏螺旋体异常特异性DNA序列可用于直接检测患者样品中的病原体（例如通过PCR反应）。

公开(公告)号：US06248538B1

公开(公告)日：2001-06-19

申请号：US08209603

申请日：1994-03-10

申请人： Manfred Motz ,  Erwin Soutscheck ,  Renate Fuchs ,  Bettina Wilske ,  Vera Preac-Mursic

发明人： Manfred Motz ,  Erwin Soutscheck ,  Renate Fuchs ,  Bettina Wilske ,  Vera Preac-Mursic

IPC分类号： G01N3353

CPC分类号： C07K16/1207 ,  A61K38/00 ,  A61K39/00 ,  C07K14/20 ,  G01N33/56911 ,  Y02A50/401 ,  Y02A50/57 ,  Y10S436/804 ,  Y10S530/825

摘要： Various immunologically active proteins from Borrelia burgdorferi have been prepared by genetic manipulation in microorganisms. To do this, the specific DNA sequences were selected from a B. burgdorferi gene bank using suitable screening methods, or were prepared directly by DNA amplification using selected hybridization probes, and were placed under the control of inducible promoters such as, for example, the lac promoter. It has been possible, owing to description of efficient purification methods for the expressed antigens, to provide the proteins in a suitable way. These proteins can be used to produce specific and sensitive diagnostic assay kits. The specific combination of the immunologically active proteins makes precise diagnosis possible. Furthermore, monoclonal antibodies have been generated and are used as reagents for detecting pathogens directly in test samples or after cultivation. The Borrelia burgdorferi-specific DNA sequences can be employed for direct detection of the pathogen in patients' samples (for example by means of the PCR reaction).

摘要翻译： 通过遗传操作在微生物中制备了来自博氏疏螺旋体的各种免疫活性蛋白质。 为此，使用合适的筛选方法从布氏疏螺旋体基因库中选择特异性DNA序列，或者通过使用选择的杂交探针的DNA扩增直接制备，并置于诱导型启动子的控制下，例如， lac启动子。 由于对表达的抗原的有效纯化方法的描述，可以以合适的方式提供蛋白质是可能的。 这些蛋白质可用于制备特异性敏感的诊断测定试剂盒。 免疫活性蛋白质的特异性组合使得精确的诊断成为可能。 此外，已经产生了单克隆抗体，并且用作用于在测试样品中或培养后直接检测病原体的试剂。 疏螺旋体异常特异性DNA序列可用于直接检测患者样品中的病原体（例如通过PCR反应）。