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    Active proteins from Borrelia burgdorferi
    6.
    发明授权
    Active proteins from Borrelia burgdorferi 失效
    来自博氏疏螺旋体的活性蛋白

    公开(公告)号：US06183755B2

    公开(公告)日：2001-02-06

    申请号：US09196293

    申请日：1998-11-19

    申请人： Manfred Motz ,  Erwin Soutscheck ,  Renate Fuchs ,  Bettina Wilske ,  Vera Preac-Mursic

    发明人： Manfred Motz ,  Erwin Soutscheck ,  Renate Fuchs ,  Bettina Wilske ,  Vera Preac-Mursic

    IPC分类号： A61K3902

    CPC分类号： C07K16/1207 ,  A61K38/00 ,  A61K39/00 ,  C07K14/20 ,  G01N33/56911 ,  Y02A50/401 ,  Y02A50/57 ,  Y10S436/804 ,  Y10S530/825

    摘要： Various immunologically active proteins from Borrelia burgdorferi have been prepared by genetic manipulation in microorganisms. To do this, the specific DNA sequences were selected from a B. burgdorferi gene bank using suitable screening methods, or were prepared directly by DNA amplification using selected hybridization probes, and were placed under the control of inducible promoters such as, for example, the lac promoter. It has been possible, owing to description of efficient purification methods for the expressed antigens, to provide the proteins in a suitable way. These proteins can be used to produce specific and sensitive diagnostic assay kits. The specific combination of the immunologically active proteins makes precise diagnosis possible. Furthermore, monoclonal antibodies have been generated and are used as reagents for detecting pathogens directly in test samples or after cultivation. The Borrelia burgdorferi-specific DNA sequences can be employed for direct detection of the pathogen in patients' samples (for example by means of the PCR reaction).

    摘要翻译： 通过遗传操作在微生物中制备了来自博氏疏螺旋体的各种免疫活性蛋白质。 为此，使用合适的筛选方法从布氏疏螺旋体基因库中选择特异性DNA序列，或者通过使用选择的杂交探针的DNA扩增直接制备，并置于诱导型启动子的控制下，例如， lac启动子。 由于对表达的抗原的有效纯化方法的描述，可以以合适的方式提供蛋白质是可能的。 这些蛋白质可用于制备特异性敏感的诊断测定试剂盒。 免疫活性蛋白质的特异性组合使得精确的诊断成为可能。 此外，已经产生了单克隆抗体，并且用作用于在测试样品中或培养后直接检测病原体的试剂。 疏螺旋体异常特异性DNA序列可用于直接检测患者样品中的病原体（例如通过PCR反应）。

    Immunologically active proteins from Borrelia burgdorferi, nucleic acids which encode them, and their use in test kits and as vaccines
    8.
    发明授权
    Immunologically active proteins from Borrelia burgdorferi, nucleic acids which encode them, and their use in test kits and as vaccines 有权
    来自博氏疏螺旋体的免疫活性蛋白，编码它们的核酸及其在测试试剂盒和疫苗中的应用

    公开(公告)号：US06610301B1

    公开(公告)日：2003-08-26

    申请号：US09242299

    申请日：1999-02-12

    申请人： Manfred Motz ,  Erwin Soutschek

    发明人： Manfred Motz ,  Erwin Soutschek

    IPC分类号： A61K3902

    CPC分类号： C07K14/20 ,  A61K39/00 ,  A61K2039/51 ,  Y02A50/401

    摘要： The present invention relates to immunologically active proteins from Borrelia burgdorferi which are present in a form which is free of other proteins derived from Borrelia burgdorferi and which exhibit the sequence of the protein 1829-22A, which has the amino acid sequence MKKFNLIIEALFAILLTACNFGLMEETKIALESSSKDVKNKILQIKKDAEDKGVNFAAFTSSETG SKVTNGGLALREAKIQAINEVEKFLKRIEEEALKLKEHGNSGQFLELFDLLLEVLESLEPIGIKG LKDFISEEAKCNPISTSERLIEVKVQIENKMEEVKRKQNLNKERKSNKGKKKK SEQ. ID NO.: 1 or a part sequence thereof having at least 10 consecutive amino acids, or exhibit the sequence of the protein 1829-22B, which has the amino acid sequence MIKYNKIILTLTLLASLLAACSLTGKARLESSVKDITNEIEKAIKEAEDAGVKTDAFTETQTGGK VAGPKIRAAKIRVADLTIKFLEATEEETITFKENGAGEDEFSGIYDLILNAAKAVEKIGMKDMTK TVEEAAKENPKTTANGIIEIVKVMKAKVENIKEKQTKNQK SEQ. ID NO.: 2 or a part sequence thereof having at least 10 consecutive amino acids.

    摘要翻译： 本发明涉及由莱姆病螺旋体免疫活性的蛋白质，其存在于一种形式，其不含来自伯氏疏螺旋和其表现出的蛋白1829-22A，其具有氨基酸sequenceMKKFNLIIEALFAILLTACNFGLMEETKIALESSSKDVKNKILQIKKDAEDKGVNFAAFTSSETG SKVTNGGLALREAKIQAINEVEKFLKRIEEEALKLKEHGNSGQFLELFDLLLEVLESLEPIGIKG LKDFISEEAKCNPISTSERLIEVKVQIENKMEEVKRKQNLNKERKSNKGKKKK SEQ的序列衍生其它蛋白质。 或具有至少10个连续氨基酸的部分序列，或表现出具有氨基酸序列的蛋白质1829-22B的序列，其具有以下氨基酸序列：MIKYNKIILTLTLLASLLAACSLTGKARLESSVKDITNEIEKAIKEAEDAGVKTDAFTETQTGGK VAGPKIRAAKIRVADLTIKFLEATEEETITFKENGAGEDEFSGIYDLILNAAKAVEKIGMKDMTK TVEEAAKENPKTTANGIIEIVKVMKAKVENIKEKQTKNQK SEQ ID NO： ID号：2或其具有至少10个连续氨基酸的部分序列。

    Immunologically active proteins from Borrelia burgdorferi
    9.
    发明授权
    Immunologically active proteins from Borrelia burgdorferi 失效
    来自博氏疏螺旋体的免疫活性蛋白

    公开(公告)号：US06509019B1

    公开(公告)日：2003-01-21

    申请号：US09711546

    申请日：2000-11-13

    申请人： Renate Fuchs ,  Bettina Wilske ,  Vera Preac-Mursic ,  Manfred Motz ,  Erwin Soutscheck

    发明人： Renate Fuchs ,  Bettina Wilske ,  Vera Preac-Mursic ,  Manfred Motz ,  Erwin Soutscheck

    IPC分类号： A61K3902

    CPC分类号： C07K16/1207 ,  A61K38/00 ,  A61K39/00 ,  C07K14/20 ,  G01N33/56911 ,  Y02A50/401 ,  Y02A50/57 ,  Y10S436/804 ,  Y10S530/825

    摘要： Various immunologically active proteins from Borrelia burgdorferi have been prepared by genetic manipulation in microorganisms. To do this, the specific DNA sequences were selected from a B. burgdorferi gene bank using suitable screening methods, or were prepared directly by DNA amplification using selected hybridization probes, and were placed under the control of inducible promoters such as, for example, the lac promoter. It has been possible, owing to description of efficient purification methods for the expressed antigens, to provide the proteins in a suitable way. These proteins can be used to produce specific and sensitive diagnostic assay kits. The specific combination of the immunologically active proteins makes precise diagnosis possible. Furthermore, monoclonal antibodies have been generated and are used as reagents for detecting pathogens directly in test samples or after cultivation. The Borrelia burgdorferi-specific DNA sequences can be employed for direct detection of the pathogen in patients' samples (for example by means of the PCR reaction).

    摘要翻译： 通过遗传操作在微生物中制备了来自博氏疏螺旋体的各种免疫活性蛋白质。 为此，使用合适的筛选方法从布氏疏螺旋体基因库中选择特异性DNA序列，或者通过使用选择的杂交探针的DNA扩增直接制备，并置于诱导型启动子的控制下，例如， lac启动子。 由于对表达的抗原的有效纯化方法的描述，可以以合适的方式提供蛋白质是可能的。 这些蛋白质可用于制备特异性敏感的诊断测定试剂盒。 免疫活性蛋白质的特异性组合使得精确的诊断成为可能。 此外，已经产生了单克隆抗体，并且用作用于在测试样品中或培养后直接检测病原体的试剂。 疏螺旋体异常特异性DNA序列可用于直接检测患者样品中的病原体（例如通过PCR反应）。

    Immunologically active peptides or polypeptides from the parvovirus B19
    10.
    发明授权
    Immunologically active peptides or polypeptides from the parvovirus B19 失效
    来自细小病毒B19的免疫活性肽或多肽

    公开(公告)号：US06274307B1

    公开(公告)日：2001-08-14

    申请号：US08856841

    申请日：1997-05-15

    申请人： Erwin Soutschek ,  Manfred Motz

    发明人： Erwin Soutschek ,  Manfred Motz

    IPC分类号： C12Q170

    CPC分类号： C07K14/005 ,  C12N2750/14222 ,  Y10S435/975

    摘要： Immunologically active peptides or polypeptides with a partial amino-acid sequence of the capsid proteins VP1 and VP2 of parvovirus B19 which permit tests to be carried out at low cost, sensitively and specifically for the determination of antibodies against human parvovirus B19 are made available. Short peptide sequences which, employed as antigen, serve to identify anti-B19 IgG-positive sera are identified. Furthermore, the production of these peptides using genetic engineering measures is disclosed. Other antigens which are produced by genetic engineering and which can be stably produced in a high yield in E.coli and subsequently purified therefrom are used as additional antigens for IgG detection. Finally, a set of antigens permits tests to be carried out to determine IgM antibodies against the virus. In addition, the components, produced by genetic engineering, of the surface proteins represent substances which can be used for prophylactic immunisation.

    摘要翻译： 提供具有细小病毒B19的衣壳蛋白VP1和VP2的部分氨基酸序列的免疫活性肽或多肽，其允许以低成本进行测试，敏感地且专门用于测定抗人细小病毒B19的抗体。 鉴定用作抗原的短肽序列，用于鉴定抗B19 IgG阳性血清。 此外，公开了使用遗传工程措施生产这些肽。 通过遗传工程产生并且可以在大肠杆菌中以高产量稳定产生并随后从其中纯化的其他抗原用作IgG检测的额外抗原。 最后，一组抗原允许进行测试来确定针对病毒的IgM抗体。 此外，通过遗传工程生产的表面蛋白质的组分代表可用于预防性免疫的物质。