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11.发明授权公开(公告)号：US10973229B2
公开(公告)日：2021-04-13
申请号：US16434995
申请日：2019-06-07
Applicant: DOW AGROSCIENCES LLC , M.S. Technologies, L.L.C.
Inventor： Yunxing C. Cui , Thomas Hoffman , Ning Zhou , Stephen N. Novak , Julissa Colon , Dawn M. Parkhurst , Sandra G. Toledo , Terry R. Wright , Sean M. Russell , Bruce Held , Vaithilingam Sekar
Abstract: This invention relates in part to soybean event pDAB8264.44.06.1 and includes a novel expression cassettes and transgenic inserts comprising multiple traits conferring resistance to glyphosate, aryloxyalkanoate, and glufosinate herbicides. This invention also relates in part to methods of controlling resistant weeds, plant breeding and herbicide tolerant plants. In some embodiments, the event sequence can be “stacked” with other traits, including, for example, other herbicide tolerance gene(s) and/or insect-inhibitory proteins. This invention further relates in part to endpoint TaqMan PCR assays for the detection of Event pDAB8264.44.06.1 in soybeans and related plant material. Some embodiments can perform high throughput zygosity analysis of plant material and other embodiments can be used to uniquely identify the zygosity of and breed soybean lines comprising the event of the subject invention. Kits and conditions useful in conducting these assays are also provided.
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12.发明授权公开(公告)号：US10450549B2
公开(公告)日：2019-10-22
申请号：US15287630
申请日：2016-10-06
Applicant: Dow AgroSciences, LLC
Inventor： Carla N. Yerkes , Barbara A. Zilinskas , Donald J. Merlo , Terry R. Wright , Justin Lira , Nicole Arnold , Gary D. Thompson , Ning Zhou , Andrew Worden
Abstract: The present disclosure relates to the use of a grass-active herbicide postemergently applied to AAD1-transformed turfgrasses to selectively control grass weeds in a turf grass crop. Also described is the use of AAD1 as a selectable marker in the production of transgenic turfgrass.
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13.发明申请公开(公告)号：US20170088855A1
公开(公告)日：2017-03-30
申请号：US15287630
申请日：2016-10-06
Applicant: Dow AgroSciences, LLC
Inventor： Carla N. Yerkes , Barbara A. Zilinskas , Donald J. Merlo , Terry R. Wright , Justin Lira , Nicole Arnold , Gary D. Thompson , Ning Zhou , Andrew Worden
CPC classification number: C12N15/8274 , C12N9/0069 , C12N9/0071 , C12N15/8209
Abstract: The present disclosure relates to the use of a grass-active herbicide postemergently applied to AAD1-transformed turfgrasses to selectively control grass weeds in a turf grass crop. Also described is the use of AAD1 as a selectable marker in the production of transgenic turfgrass.
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14.发明授权公开(公告)号：US11819022B2
公开(公告)日：2023-11-21
申请号：US17897988
申请日：2022-08-29
Applicant: Dow AgroSciences LLC , M.S. Technologies, L.L.C.
Inventor： Yunxing C. Cui , Thomas Hoffman , Ning Zhou , Stephen N. Novak , Julissa Colon , Dawn M. Parkhurst , Sandra G. Toledo , Terry R. Wright , Sean M. Russell , Bruce Held , Vaithilingam Sekar
IPC: C12N15/82 , A01N39/04 , C12N9/10 , C12Q1/6895 , C12N9/00
CPC classification number: A01N39/04 , C12N9/1092 , C12N9/93 , C12N15/8241 , C12N15/8274 , C12N15/8275 , C12Q1/6895 , C12Y205/01019 , C12Y604/01002 , C12Q2600/13 , C12Q2600/156 , C12Q2600/16
Abstract: This invention relates in part to soybean event pDAB8264.44.06.1 and includes a novel expression cassettes and transgenic inserts comprising multiple traits conferring resistance to glyphosate, aryloxyalkanoate, and glufosinate herbicides. This invention also relates in part to methods of controlling resistant weeds, plant breeding and herbicide tolerant plants. In some embodiments, the event sequence can be “stacked” with other traits, including, for example, other herbicide tolerance gene(s) and/or insect-inhibitory proteins. This invention further relates in part to endpoint TaqMan PCR assays for the detection of Event pDAB8264.44.06.1 in soybeans and related plant material. Some embodiments can perform high throughput zygosity analysis of plant material and other embodiments can be used to uniquely identify the zygosity of and breed soybean lines comprising the event of the subject invention. Kits and conditions useful in conducting these assays are also provided.
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15.发明申请公开(公告)号：US20230056359A1
公开(公告)日：2023-02-23
申请号：US17876172
申请日：2022-07-28
Applicant: Dow AgroSciences LLC , M.S. Technologies, L.L.C.
Inventor： Yunxing C. Cui , Thomas Hoffman , Ning Zhou , Stephen N. Novak , Julissa Colon , Dawn M. Parkhurst , Sandra G. Toledo , Terry R. Wright , Sean M. Russell , Bruce Held , Vaithilingam Sekar
Abstract: This invention relates in part to soybean event pDAB8264.44.06.1 and includes a novel expression cassettes and transgenic inserts comprising multiple traits conferring resistance to glyphosate, aryloxyalkanoate, and glufosinate herbicides. This invention also relates in part to methods of controlling resistant weeds, plant breeding and herbicide tolerant plants. In some embodiments, the event sequence can be “stacked” with other traits, including, for example, other herbicide tolerance gene(s) and/or insect-inhibitory proteins. This invention further relates in part to endpoint TaqMan PCR assays for the detection of Event pDAB8264.44.06.1 in soybeans and related plant material. Some embodiments can perform high throughput zygosity analysis of plant material and other embodiments can be used to uniquely identify the zygosity of and breed soybean lines comprising the event of the subject invention. Kits and conditions useful in conducting these assays are also provided.
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Patent Agency Ranking
公开(公告)号：US10344322B2
公开(公告)日：2019-07-09
申请号：US15249684
申请日：2016-08-29
Applicant: Dow AgroSciences LLC
Inventor： Lakshmi Sastry-Dent , Matthew A. Simpson , Zehui Cao , Wei Chen , Ning Zhou , Steven R. Webb
IPC: C12Q1/68 , C12Q1/6858 , C12Q1/6895 , C12N15/10 , C12N15/66 , C12N15/70 , C40B40/08 , A01G24/00 , C12N5/00
Abstract: The present disclosure provides methods for detecting and identifying plant events that contain precision targeted genomic loci, and plants and plant cells comprising such targeted genomic loci. The method can be deployed as a high throughput process utilized for screening the intactness or disruption of a targeted genomic loci and optionally for detecting a donor DNA polynucleotide insertion at the targeted genomic loci. The methods are readily applicable for the identification of plant events produced via a targeting method which results from the use of a site specific nuclease.
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公开(公告)号：US09863008B2
公开(公告)日：2018-01-09
申请号：US14410793
申请日：2013-06-25
Applicant: DOW AGROSCIENCES LLC
Inventor： Lauren Clark , Kelley Ann Smith , Yang Wang , Ning Zhou
CPC classification number: C12Q1/6895 , C12N15/8277 , C12N15/8286 , C12Q2600/13 , C12Q2600/156 , Y02A40/162
Abstract: Soybean Event pDAB9582.816.15.1 comprises gene expression cassettes which contain genes encoding Cry1F, Cry1Ac (synpro), and PAT, affording insect resistance and herbicide tolerance to soybean crops containing the event, and enabling methods for crop protection and protection of stored products. The disclosure provides polynucleotide related event detection methods. The present disclosure relates to a method for detecting a new insect resistant and herbicide tolerant transgenic soybean transformation event, designated Soybean Event pDAB9582.816.15,1. The DNA of soybean plants containing this event includes the junction/flanking sequences described herein that characterize the location of the inserted DNA within the soybean genome. SEQ ID NO: 1 and SEQ ID N0:2 are diagnostic for Soybean Event pDAB9582.816.15.1.
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公开(公告)号：US09695441B2
公开(公告)日：2017-07-04
申请号：US14223249
申请日：2014-03-24
Applicant: DOW AGROSCIENCES LLC
Inventor： Nathan Bard , Gregory A. Bradfisch , Yunxing C. Cui , James E. Dripps , Thomas Hoffman , Dayakar Pareddy , Dawn M. Parkhurst , Sandra G. Toledo , Barry Wiggins , Ning Zhou
CPC classification number: C12N15/8286 , A01N37/38 , A01N37/44 , A01N65/20 , C12N15/8209 , C12N15/8274 , C12N15/8277 , C12Q1/6895 , C12Q2600/13 , Y02A40/162
Abstract: Soybean event 9582.814.19.1 comprising genes encoding Cry1F, Cry1Ac (synpro), and PAT, affording insect resistance and herbicide tolerance to soybean crops containing the event, and enabling methods for crop protection and protection of stored products.
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公开(公告)号：US20130130920A1
公开(公告)日：2013-05-23
申请号：US13683442
申请日：2012-11-21
Applicant: Dow AgroSciences LLC
Inventor： Zehui Cao , Stephen Novak , Lakshmi Sastry-Dent , Ning Zhou
IPC: C12Q1/68
CPC classification number: C12Q1/686 , C12Q1/6895
Abstract: A method of analyzing, in chromosomal DNA having a transgene incorporated therein, a DNA flanking region derived from the chromosome which is adjacent to the transgene. Wherein, the DNA flanking region is characterized by isolation and digestion of genomic DNA with a restriction enzyme, ligation of a double stranded adapter to the isolated and digested genomic DNA, a primer extension reaction of the adapter ligated genomic DNA, and the isolation of the primer extension reaction product via a streptavidin-biotin interaction. The DNA flanking region is further characterized via subsequent PCR amplification reactions and DNA sequencing.
Abstract translation: 在其中掺入转基因的染色体DNA中分析衍生自与转基因相邻的染色体的DNA侧翼区的方法。 其中DNA侧翼区的特征在于用限制酶分离和消化基因组DNA，将双链衔接子连接到分离和消化的基因组DNA上，衔接子连接的基因组DNA的引物延伸反应和 通过链霉亲和素 - 生物素相互作用引物延伸反应产物。 通过随后的PCR扩增反应和DNA测序进一步表征DNA侧翼区。
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20.发明公开公开(公告)号：US20230143169A1
公开(公告)日：2023-05-11
申请号：US17897988
申请日：2022-08-29
Applicant: Dow AgroSciences LLC , M.S. Technologies, L.L.C.
Inventor： Yunxing C. CUI , Thomas Hoffman , Ning Zhou , Stephen N. Novak , Julissa Colon , Dawn M. Parkhursl , Sandra G. Toledo , Terry R. Wright , Sean M. Russell , Bruce Held , Vaithilingam Sekar
CPC classification number: A01N39/04 , C12N9/1092 , C12Y205/01019 , C12N15/8274 , C12Q1/6895 , C12N15/8275 , A01H1/02 , C12N9/93 , C12N15/8241 , C12Y604/01002 , C12Q2600/16 , C12Q2600/13 , C12Q2600/156
Abstract: This invention relates in part to soybean event pDAB8264.44.06.1 and includes a novel expression cassettes and transgenic inserts comprising multiple traits conferring resistance to glyphosate, aryloxyalkanoate, and glufosinate herbicides. This invention also relates in part to methods of controlling resistant weeds, plant breeding and herbicide tolerant plants. In some embodiments, the event sequence can be “stacked” with other traits, including, for example, other herbicide tolerance gene(s) and/or insect-inhibitory proteins. This invention further relates in part to endpoint TaqMan PCR assays for the detection of Event pDAB8264.44.06.1 in soybeans and related plant material. Some embodiments can perform high throughput zygosity analysis of plant material and other embodiments can be used to uniquely identify the zygosity of and breed soybean lines comprising the event of the subject invention. Kits and conditions useful in conducting these assays are also provided.
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