公开(公告)号：US20170283865A1

公开(公告)日：2017-10-05

申请号：US15249684

申请日：2016-08-29

Applicant: Dow AgroSciences LLC

Inventor： Lakshmi Sastry-Dent ,  Matthew A. Simpson ,  Zehui Cao ,  Wei Chen ,  Ning Zhou ,  Steven R. Webb

IPC: C12Q1/68 ,  A01G31/00 ,  C12N15/70 ,  C12N15/66 ,  C40B40/08 ,  C12N15/10

CPC classification number: C12Q1/6858 ,  A01G24/00 ,  C12N5/00 ,  C12N15/1082 ,  C12N15/66 ,  C12N15/70 ,  C12Q1/6895 ,  C12Q2600/13 ,  C12Q2600/156 ,  C40B40/08

Abstract: The present disclosure provides methods for detecting and identifying plant events that contain precision targeted genomic loci, and plants and plant cells comprising such targeted genomic loci. The method can be deployed as a high throughput process utilized for screening the intactness or disruption of a targeted genomic loci and optionally for detecting a donor DNA polynucleotide insertion at the targeted genomic loci. The methods are readily applicable for the identification of plant events produced via a targeting method which results from the use of a site specific nuclease.

公开(公告)号：US20140298547A1

公开(公告)日：2014-10-02

申请号：US14105223

申请日：2013-12-13

Applicant: Dow AgroSciences LLC

Inventor： Lakshmi Sastry-Dent ,  Matthew Simpson ,  Zehui Cao ,  Wei Chen ,  Ning Zhou ,  Steven R. Webb

IPC: C12Q1/68

CPC classification number: C12Q1/6858 ,  A01G24/00 ,  C12N5/00 ,  C12N15/1082 ,  C12N15/66 ,  C12N15/70 ,  C12Q1/6895 ,  C12Q2600/13 ,  C12Q2600/156 ,  C40B40/08

Abstract: The present disclosure provides methods for detecting and identifying plant events that contain precision targeted genomic loci, and plants and plant cells comprising such targeted genomic loci. The method can be deployed as a high throughput process utilized for screening the intactness or disruption of a targeted genomic loci and optionally for detecting a donor DNA polynucleotide insertion at the targeted genomic loci. The methods are readily applicable for the identification of plant events produced via a targeting method which results from the use of a site specific nuclease.

Abstract translation: 本公开提供了用于检测和鉴定含有精确靶向基因组基因座的植物事件的方法，以及包含这种靶基因组基因座的植物和植物细胞。 该方法可以部署为用于筛选靶基因组基因座的完整性或破坏的高通量过程，并且任选地用于检测目标基因组基因座上的供体DNA多核苷酸插入。 该方法容易适用于鉴定通过使用位点特异性核酸酶产生的靶向方法产生的植物事件。

公开(公告)号：US11198882B2

公开(公告)日：2021-12-14

申请号：US16112200

申请日：2018-08-24

Applicant: Dow AgroSciences LLC

Inventor： Lakshmi Sastry-Dent ,  Zehui Cao ,  Shreedharan Sriram ,  Steven R. Webb ,  Debra L. Camper

IPC: C12N15/82 ,  A01H5/10

Abstract: As disclosed herein, optimal native genomic loci from maize plants have been identified that represent best sites for targeted insertion of exogenous sequences.

公开(公告)号：US11149287B2

公开(公告)日：2021-10-19

申请号：US16246864

申请日：2019-01-14

Applicant: Dow AgroSciences LLC

Inventor： Lakshmi Sastry-Dent ,  Zehui Cao ,  Shreedharan Sriram ,  Steven R. Webb ,  Debra L. Camper

IPC: C12N15/82 ,  C12N15/90

Abstract: As disclosed herein, optimal native genomic loci have been identified in dicot plants, such as soybean plants, that represent best sites for targeted insertion of exogenous sequences.

公开(公告)号：US10233465B2

公开(公告)日：2019-03-19

申请号：US14531732

申请日：2014-11-03

Applicant: Dow AgroSciences LLC

Inventor： Lakshmi Sastry-Dent ,  Zehui Cao ,  Shreedharan Sriram ,  Steven R. Webb ,  Debra L. Camper

IPC: C12N15/82 ,  C12N15/90

Abstract: As disclosed herein, optimal native genomic loci have been identified in dicot plants, such as soybean plants, that represent best sites for targeted insertion of exogenous sequences.

公开(公告)号：US10106804B2

公开(公告)日：2018-10-23

申请号：US15867770

申请日：2018-01-11

Applicant: DOW AGROSCIENCES LLC

Inventor： Lakshmi Sastry-Dent ,  Zehui Cao ,  Shreedharan Sriram ,  Steven R. Webb ,  Debra L. Camper ,  W. Michael Ainley

IPC: C12N15/82 ,  A01H5/10

Abstract: As disclosed herein, optimal native genomic loci of soybean plants have been identified that represent best sites for targeted insertion of exogenous sequences.

公开(公告)号：US10087492B2

公开(公告)日：2018-10-02

申请号：US15705447

申请日：2017-09-15

Applicant: DOW AGROSCIENCES LLC

Inventor： Lakshmi Sastry-Dent ,  W. Michael Ainley ,  Jayakumar P. Samuel ,  Zehui Cao ,  Liu Shen ,  Cristie M. Dewes

IPC: C12P19/34 ,  C12Q1/6895

Abstract: The present disclosure provides a system and methods for detecting and identifying plant events that contain donor sequences inserted precisely into a targeted genomic loci, and plants and plant cells comprising such targeted genomic loci. The method comprises the steps of amplifying a genomic DNA with a first round of PCR to produce an amplicon from donor sequences inserted in the reverse orientation, wherein the production of the amplicon indicates the presence of the site specific integration event.

公开(公告)号：US20130211729A1

公开(公告)日：2013-08-15

申请号：US13761711

申请日：2013-02-07

Applicant: Dow Agrosciences LLC

Inventor： Lakshmi Sastry-Dent ,  Shreedharan Sriram ,  Navin Elango ,  Zehui Cao ,  Karthik Narayan Muthuraman

IPC: G06F19/28

CPC classification number: G16B50/00 ,  G16B30/00

Abstract: Systems and methods for data analysis are provided. In one embodiment, a method for analysis is provided, including electronically receiving sequence data; electronically receiving one or more reference data sequences related to at least an expression vector; associating the sequence data with at least one of the reference data sequences to identify a transgene flanking sequence; searching a genome for one or more insertion sites of the transgene flanking sequence; and annotating the genome and the one or more insertion sites within the genome when one or more insertion sites are found in said searching step.

Abstract translation: 提供了数据分析的系统和方法。 在一个实施例中，提供了一种用于分析的方法，包括电子地接收序列数据; 电子地接收与至少一个表达载体相关的一个或多个参考数据序列; 将序列数据与至少一个参考数据序列相关联以鉴定转基因侧翼序列; 在基因组中搜索转基因侧翼序列的一个或多个插入位点; 并且当在所述搜索步骤中发现一个或多个插入位点时，注释基因组和基因组内的一个或多个插入位点。