公开(公告)号：US10597689B2

公开(公告)日：2020-03-24

申请号：US16173805

申请日：2018-10-29

Applicant: DSM IP ASSETS B.V.

Inventor： Bertus Noordam ,  Michael Petrus Jozef Berkhout

IPC: C12P19/14 ,  C12P19/02 ,  C13K1/02 ,  C13K13/00 ,  D21C3/26

Abstract: The invention relates to a process for the preparation of a fermentation product from lignocellulosic material, comprising the following steps: a) optionally, pre-treatment of the lignocellulosic material, b) optionally, washing of the optionally pretreated lignocellulosic material, c) enzymatic hydrolysis of the optionally washed and/or optionally pretreated lignocellulosic material using an enzyme composition comprising at least two cellulases and whereby the enzyme composition at least comprises LPMO, and optionally purifying the hydrolyzed lignocellulosic material, d) fermentation of the hydrolyzed lignocellulosic material to produce a fermentation product, and e) optionally, recovery of a fermentation product, wherein oxygen is consumed in amounts corresponding to between 20 and 5000 mmol molecular oxygen per kg glucan present in the lignocellulosic material, the oxygen is added after the pretreatment and before and/or during the enzymatic hydrolysis of the lignocellulosic material, preferably in an amount corresponding to at least 30 mmol molecular oxygen per kg glucan present in the lignocellulosic material, more preferably in an amount corresponding to at least 40 mmol molecular oxygen per kg glucan present in the lignocellulosic material, and most preferably in an amount corresponding to at least 50 mmol molecular oxygen per kg glucan present in the lignocellulosic material is consumed.

公开(公告)号：US10144939B2

公开(公告)日：2018-12-04

申请号：US15307046

申请日：2015-04-29

Applicant: DSM IP ASSETS B.V.

Inventor： Bertus Noordam ,  Michael Petrus Jozef Berkhout

IPC: C12P19/14 ,  C12P19/02 ,  C13K1/02 ,  D21C3/26 ,  C13K13/00

Abstract: The invention relates to a process for the preparation of a fermentation product from lignocellulosic material, comprising the following steps: a) optionally, pre-treatment of the lignocellulosic material, b) optionally, washing of the optionally pretreated lignocellulosic material, c) enzymatic hydrolysis of the optionally washed and/or optionally pretreated lignocellulosic material using an enzyme composition comprising at least two cellulases and whereby the enzyme composition at least comprises LPMO, and optionally purifying the hydrolyzed lignocellulosic material, d) fermentation of the hydrolyzed lignocellulosic material to produce a fermentation product, and e) optionally, recovery of a fermentation product, wherein oxygen is consumed in amounts corresponding to between 20 and 5000 mmol molecular oxygen per kg glucan present in the lignocellulosic material, the oxygen is added after the pretreatment and before and/or during the enzymatic hydrolysis of the lignocellulosic material, preferably in an amount corresponding to at least 30 mmol molecular oxygen per kg glucan present in the lignocellulosic material, more preferably in an amount corresponding to at least 40 mmol molecular oxygen per kg glucan present in the lignocellulosic material, and most preferably in an amount corresponding to at least 50 mmol molecular oxygen per kg glucan present in the lignocellulosic material is consumed.

公开(公告)号：US10087475B2

公开(公告)日：2018-10-02

申请号：US15300278

申请日：2015-01-29

Applicant: DSM IP Assets B.V.

Inventor： Bertus Noordam ,  Michael Petrus Jozef Berkhout ,  Joseph Johannes Maria Hofmeester

IPC: C12P19/02 ,  C12P19/14 ,  C12M1/00 ,  D21C3/26 ,  D21C5/00

Abstract: The disclosure relates to a process for the preparation of a sugar product from lignocellulosic material, comprising the following steps: a) optionally, pretreatment of the lignocellulosic material; b) optionally, washing of the optionally pretreated lignocellulosic material; c) enzymatic hydrolysis of the optionally washed and/or optionally pretreated lignocellulosic material in a hydrolysis reactor using an enzyme composition comprising at least two cellulase; and d) optionally, recovery of a sugar product; wherein during the enzymatic hydrolysis oxygen-containing gas is added to the lignocellulosic material in the hydrolysis reactor and wherein part of the oxygen-containing gas, added to the lignocellulosic material, is gas originating from the headspace of the reactor, preferably during part of the time of the enzymatic hydrolysis less oxygen is added to the lignocellulosic material compared to the other part of the time of the enzymatic hydrolysis, or preferably during part of the time of the enzymatic hydrolysis no oxygen is added to the lignocellulosic material.

公开(公告)号：US11512334B2

公开(公告)日：2022-11-29

申请号：US17115429

申请日：2020-12-08

Applicant: DSM IP ASSETS B.V.

Inventor： Bertus Noordam ,  Michael Petrus Jozef Berkhout

IPC: C12P7/14 ,  C12P19/02 ,  C13K1/02 ,  D21C3/26 ,  C13K13/00 ,  C12P19/14

Abstract: The invention relates to a process for the preparation of a fermentation product from lignocellulosic material, comprising the following steps: a) optionally, pre-treatment of the lignocellulosic material, b) optionally, washing of the optionally pretreated lignocellulosic material, c) enzymatic hydrolysis of the optionally washed and/or optionally pretreated lignocellulosic material using an enzyme composition comprising at least two cellulases and whereby the enzyme composition at least comprises LPMO, and optionally purifying the hydrolysed lignocellulosic material, d) fermentation of the hydrolysed lignocellulosic material to produce a fermentation product, and e) optionally, recovery of a fermentation product, wherein oxygen is consumed in amounts corresponding to between 20 and 5000 mmol molecular oxygen per kg glucan present in the lignocellulosic material, the oxygen is added after the pretreatment and before and/or during the enzymatic hydrolysis of the lignocellulosic material, preferably in an amount corresponding to at least 30 mmol molecular oxygen per kg glucan present in the lignocellulosic material, more preferably in an amount corresponding to at least 40 mmol molecular oxygen per kg glucan present in the lignocellulosic material, and most preferably in an amount corresponding to at least 50 mmol molecular oxygen per kg glucan present in the lignocellulosic material is consumed.

公开(公告)号：US11434508B2

公开(公告)日：2022-09-06

申请号：US16856143

申请日：2020-04-23

Applicant: DSM IP ASSETS B.V.

Inventor： Bertus Noordam ,  Michael Petrus Jozef Berkhout

IPC: C12P7/10 ,  C12P7/16 ,  C12P7/18 ,  C12P7/20 ,  C12P7/40 ,  C12P7/42 ,  C12P7/46 ,  C12P7/48 ,  C12P7/54 ,  C12P7/56 ,  C12P17/10 ,  C12P19/02 ,  C12P19/14 ,  C12P35/00 ,  C12P5/02

Abstract: The invention relates to a process for the preparation of a fermentation product from ligno-cellulosic material, comprising the following steps: a) optionally pre-treatment of the ligno-cellulosic material; b) optionally washing of the optionally pre-treated ligno-cellulosic material; c) enzymatic hydrolysis of the optionally washed and/or optionally pre-treated ligno-cellulosic material using an enzyme composition comprising at least two cellulase and whereby the enzyme composition at least comprises GH61; d) whereby less than 7.5 mg enzyme composition/g glucan (on dry matter and enzyme as protein) or less than 3.0 mg enzyme composition/g feedstock (on dry matter and enzyme as protein) is used; and e) fermentation of the hydrolysed ligno-cellulosic material to produce a fermentation product; and f) optionally recovery of a fermentation product; wherein before and/or during the enzymatic hydrolysis oxygen is added to the ligno-cellulosic material.

公开(公告)号：US10717995B2

公开(公告)日：2020-07-21

申请号：US16152197

申请日：2018-10-04

Applicant: DSM IP ASSETS B.V.

Inventor： Bertus Noordam ,  Michael Petrus Jozef Berkhout

IPC: C12P7/10 ,  C12P7/16 ,  C12P7/18 ,  C12P7/20 ,  C12P7/40 ,  C12P7/42 ,  C12P7/46 ,  C12P7/48 ,  C12P7/54 ,  C12P7/56 ,  C12P17/10 ,  C12P19/02 ,  C12P19/14 ,  C12P35/00 ,  C12P5/02

Abstract: The invention relates to a process for the preparation of a fermentation product from ligno-cellulosic material, comprising the following steps: a) optionally pre-treatment of the ligno-cellulosic material; b) optionally washing of the optionally pre-treated ligno-cellulosic material; c) enzymatic hydrolysis of the optionally washed and/or optionally pre-treated ligno-cellulosic material using an enzyme composition comprising at least two cellulase and whereby the enzyme composition at least comprises GH61; d) whereby less than 7.5 mg enzyme composition/g glucan (on dry matter and enzyme as protein) or less than 3.0 mg enzyme composition/g feedstock (on dry matter and enzyme as protein) is used; and e) fermentation of the hydrolysed ligno-cellulosic material to produce a fermentation product; and f) optionally recovery of a fermentation product; wherein before and/or during the enzymatic hydrolysis oxygen is added to the ligno-cellulosic material.

公开(公告)号：US10557157B2

公开(公告)日：2020-02-11

申请号：US15535809

申请日：2015-12-16

Applicant: DSM IP ASSETS B.V.

Inventor： Bertus Noordam

IPC: C12P19/14 ,  C12P7/10 ,  C12P7/14 ,  C12P19/02 ,  C12N9/30

Abstract: The invention relates to a process for the preparation of a sugar and/or fermentation product from lignocellulosic material.

公开(公告)号：US10131923B2

公开(公告)日：2018-11-20

申请号：US15956841

申请日：2018-04-19

Applicant: DSM IP ASSETS B.V.

Inventor： Bertus Noordam ,  Michael Petrus Jozef Berkhout

IPC: C12P7/10 ,  C12P7/16 ,  C12P7/18 ,  C12P7/20 ,  C12P7/40 ,  C12P7/42 ,  C12P7/46 ,  C12P7/48 ,  C12P7/54 ,  C12P7/56 ,  C12P17/10 ,  C12P19/02 ,  C12P19/14 ,  C12P5/02 ,  C12P35/00

Abstract: The invention relates to a process for the preparation of a fermentation product from ligno-cellulosic material, comprising the following steps: a) optionally pre-treatment of the ligno-cellulosic material; b) optionally washing of the optionally pre-treated ligno-cellulosic material; c) enzymatic hydrolysis of the optionally washed and/or optionally pre-treated ligno-cellulosic material using an enzyme composition comprising at least two cellulase and whereby the enzyme composition at least comprises GH61; d) whereby less than 7.5 mg enzyme composition/g glucan (on dry matter and enzyme as protein) or less than 3.0 mg enzyme composition/g feedstock (on dry matter and enzyme as protein) is used; and e) fermentation of the hydrolyzed ligno-cellulosic material to produce a fermentation product; and f) optionally recovery of a fermentation product; wherein before and/or during the enzymatic hydrolysis oxygen is added to the ligno-cellulosic material.

公开(公告)号：US09957528B2

公开(公告)日：2018-05-01

申请号：US14440658

申请日：2013-11-07

Applicant: DSM IP ASSETS B.V.

Inventor： Bertus Noordam ,  Michael Petrus Jozef Berkhout ,  Aida Hiseni

IPC: C12P7/10 ,  C12P7/16 ,  C12P7/18 ,  C12P7/20 ,  C12P7/40 ,  C12P7/42 ,  C12P7/46 ,  C12P7/48 ,  C12P7/54 ,  C12P7/56 ,  C12P17/10 ,  C12P19/02 ,  C12P19/14 ,  C12P35/00 ,  C12P5/02

CPC classification number: C12P7/10 ,  C12P5/026 ,  C12P7/16 ,  C12P7/18 ,  C12P7/20 ,  C12P7/40 ,  C12P7/42 ,  C12P7/46 ,  C12P7/48 ,  C12P7/54 ,  C12P7/56 ,  C12P17/10 ,  C12P19/02 ,  C12P19/14 ,  C12P35/00 ,  C12P2203/00 ,  Y02E50/16 ,  Y02E50/343

Abstract: The invention relates to a process for the preparation of a sugar product from ligno-cellulosic material, comprising the following steps: a) optionally pre-treatment of the ligno-cellulosic material; b) optionally washing of the optionally pre-treated ligno-cellulosic material; c) enzymatic hydrolysis of the optionally washed and/or optionally pre-treated ligno-cellulosic material using an enzyme composition comprising at least two cellulase and whereby the enzyme composition at least comprises GH61; and d) optionally recovery of a sugar product; wherein during part of the time of the enzymatic hydrolysis, oxygen is added to the ligno-cellulosic material and during part of the time of the enzymatic hydrolysis less oxygen is added to the ligno-cellulosic material compared to the other part of the time of the enzymatic hydrolysis, preferably no oxygen is added to the ligno-cellulosic material.

公开(公告)号：US11427844B2

公开(公告)日：2022-08-30

申请号：US16859618

申请日：2020-04-27

Applicant: DSM IP ASSETS B.V.

Inventor： Bertus Noordam

IPC: C12P7/10 ,  C12P7/16 ,  C12P7/18 ,  C12P7/20 ,  C12P7/40 ,  C12P19/02 ,  C12P19/14

Abstract: The invention relates to a process for the preparation of a fermentation product from ligno-cellulosic material, comprising the following steps: a) optionally pre-treatment of the ligno-cellulosic material; b) optionally washing of the optionally pre-treated ligno-cellulosic material; c) enzymatic hydrolysis of the optionally washed and/or optionally pre-treated ligno-cellulosic material using an enzyme composition comprising at least two cellulase and whereby the enzyme composition at least comprises GH61; d) fermentation of the hydrolysed ligno-cellulosic material to produce a fermentation product; and e) optionally recovery of a fermentation product; wherein before and/or during the enzymatic hydrolysis oxygen is added to the ligno-cellulosic material.