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    YEAST CELL CAPABLE OF SIMULTANEOUSLY FERMENTING HEXOSE AND PENTOSE SUGARS
    11.
    发明申请
    YEAST CELL CAPABLE OF SIMULTANEOUSLY FERMENTING HEXOSE AND PENTOSE SUGARS 审中-公开

    公开(公告)号：US20200377846A1

    公开(公告)日：2020-12-03

    申请号：US16966741

    申请日：2019-01-31

    Applicant: DSM IP ASSETS B.V.

    Inventor： Antonius Jeroen Adriaan VAN MARIS ,  Jacobus Thomas PRONK ,  Maarten Dirk VERHOEVEN ,  Ioannis PAPAPETRIDIS

    IPC: C12N1/18 ,  C12N15/52 ,  C12N9/90 ,  C12N9/12 ,  C12N9/92 ,  C12N9/04 ,  C12N1/22 ,  C12P1/02

    Abstract: A method for preparing a yeast capable of simultaneously fermenting a pentose and a hexose sugar, comprising: (a) providing a yeast comprising: one or more heterologous genes encoding an enzyme of a pentose metabolic pathway; disruptions of a gene encoding a ribulose-phosphate 3-epimerase and of a gene encoding a glucose-6-phosphate isomerase; one or more overexpressed endogenous genes encoding an enzyme of the pentose phosphate pathway; and a disruption of one or more genes encoding an NADPH dependent 6-phosphogluconate dehydrogenase, (b) subjecting said yeast to evolutionary engineering on a medium comprising a hexose sugar and at least one pentose sugar, selecting for a yeast with improved growth rate obtain an evolved yeast; (d) restoring, in the evolved yeast, one or more of the disrupted genes, or: (d′) identifying genetic permutations in at least part of the genome of the evolved yeast by genome sequencing; (e) constructing an improved pentose and hexose-fermenting yeast comprising one or more said genetic permutations. Also described is a recombinant yeast comprising one or more heterologous genes of a pentose metabolic pathway, and a gene encoding a variant of a parent polypeptide, the variant comprising an amino acid sequence comprising at least one mutation, when aligned with the amino acid sequence in SEQ ID NO: 6.

    METABOLIC ENGINEERING OF XYLOSE-FERMENTING EUKARYOTIC CELLS
    14.
    发明申请
    METABOLIC ENGINEERING OF XYLOSE-FERMENTING EUKARYOTIC CELLS 审中-公开
    XYLOSE发酵的真核细胞的代谢工程

    公开(公告)号：US20150010943A1

    公开(公告)日：2015-01-08

    申请号：US14313025

    申请日：2014-06-24

    Applicant: DSM IP ASSETS B.V.

    Inventor： Aaron Adriaan WINKLER ,  Sipko Maarten KUYPER ,  Wilhelmus Theodorus Antonius Maria DE LAAT ,  Johannes Pieter VAN DIJKEN ,  Jacobus Thomas PRONK

    IPC: C12P7/06 ,  C12N9/90

    CPC classification number: C12P7/06 ,  C12N1/18 ,  C12N9/90 ,  C12P7/00 ,  C12P7/08 ,  Y02E50/17

    Abstract: The present invention relates to genetic modifications in eukaryotic host cells that have been transformed to express a xylose isomerase that confers on the host cell the ability to isomerize xylose to xylulose. These genetic modifications are aimed at improving the efficiency of xylose metabolism and include, e.g., reduction of nonspecific aldose reductase activity, increased xylulose kinase activity and increased flux of the pentose phosphate pathway. The modified host cells of the invention are suitable for the production of a wide variety of fermentation products, including ethanol, in fermentation processes in which a source of xylose or a source of xylose and glucose are used as carbon source.

    Abstract translation: 本发明涉及真核宿主细胞中的遗传修饰，其已被转化以表达木糖异构酶，赋予宿主细胞将木糖异构化成木酮糖的能力。 这些遗传修饰旨在提高木糖代谢的效率，并且包括例如降低非特异性醛糖还原酶活性，增加的木酮糖激酶活性和增加戊糖磷酸途径的通量。 本发明的修饰的宿主细胞适用于在发酵过程中生产各种各样的发酵产物，包括乙醇，其中木糖来源或木糖和葡萄糖来源用作碳源。

    RECOMBINANT YEAST CELL
    15.
    发明申请
    RECOMBINANT YEAST CELL 审中-公开

    公开(公告)号：US20200157489A1

    公开(公告)日：2020-05-21

    申请号：US16621572

    申请日：2018-06-04

    Applicant: DSM IP Assets B.V.

    Inventor： Ioannis PAPAPETRIDIS ,  Jacobus Thomas PRONK

    IPC: C12N1/16 ,  C12N15/52 ,  C12P7/10 ,  C12R1/865

    Abstract: The present invention describes a recombinant yeast cell functionally expressing one or more heterologous nucleic acid sequences encoding for ribulose-1,5-phosphate carboxylase/oxygenase (EC4.1.1.39; Rubisco), and optionally one or more molecular chaperones for Rubisco, and one or more phosphoribulokinase (EC2.7.1.19; PRK), wherein one or more genes of the non-oxidative branch of the pentose phosphate pathway are overexpressed and/or wherein said yeast cell comprises a deletion or disruption of a glycerol-3-phosphate dehydrogenase (GPD) gene.

    IMPROVED YEAST STRAINS FOR ETHANOL PRODUCTION
    16.
    发明申请
    IMPROVED YEAST STRAINS FOR ETHANOL PRODUCTION 审中-公开

    公开(公告)号：US20200095536A1

    公开(公告)日：2020-03-26

    申请号：US16471367

    申请日：2017-12-18

    Applicant: DSM IP Assets B.V.

    Inventor： Ioannis PAPAPETRIDIS ,  Antonius Jeroen Adriaan VAN MARIS ,  Jacobus Thomas PRONK

    IPC: C12N1/18 ,  C12N9/04 ,  C12N9/02 ,  C12P7/10

    Abstract: This invention relates to a recombinant cell, preferably a recombinant yeast cell comprising: a) a gene coding for an enzyme having glycerol-3-phosphate dehydrogenase activity, wherein said enzyme has a cofactor dependency for at least NADP+ and/or for NADPH; b) a gene encoding an enzyme having at least NAD+ dependent acetylating acetaldehyde dehydrogenase activity (EC 1.2.1.10); and c) a mutation or disruption in at least one gene selected from the group of GPD1 and GPD2. Said cell is suitable for ethanol production, has a reduced glycerol production at high ethanol yield.

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