公开(公告)号：US08497100B2

公开(公告)日：2013-07-30

申请号：US13762327

申请日：2013-02-07

Applicant: DSM IP Assets B.V.

Inventor： Paul W. Behrens ,  John M. Thompson ,  Kirk Apt ,  Joseph W. Pfeifer, III ,  James P. Wynn ,  James Casey Lippmeier ,  Jaouad Fichtali ,  Jon Hansen

IPC: C12P7/64 ,  C12N1/12 ,  C11B1/00 ,  A23D9/02 ,  A23L1/30

Abstract: Methods for production of highly unsaturated fatty acids by marine microorganisms, including the heterotrophic marine dinoflagellate Crypthecodinium, using low levels of chloride ion are disclosed. Specifically, methods of increasing production of highly unsaturated fatty acids by marine microorganisms while growing in low chloride media by manipulating sodium ion and potassium ion levels. The invention also relates to methods of production of highly unsaturated fatty acids by marine organisms at low pH levels, and includes methods for generation of low pH tolerant strains.

公开(公告)号：US20130158280A1

公开(公告)日：2013-06-20

申请号：US13762327

申请日：2013-02-07

Applicant: DSM IP Assets B.V.

Inventor： Paul W. BEHRENS ,  John M. Thompson ,  Kirk Apt ,  Joseph W. Pfeifer, III ,  James P. Wynn ,  James Casey Lippmeier ,  Jaouad Fichtali ,  Jon Hansen

IPC: C12P7/64

CPC classification number: C12P7/6427 ,  A61K36/02 ,  C11B1/10 ,  C12P7/6409 ,  C12P7/6472

Abstract: Methods for production of highly unsaturated fatty acids by marine microorganisms, including the heterotrophic marine dinoflagellate Crypthecodinium, using low levels of chloride ion are disclosed. Specifically, methods of increasing production of highly unsaturated fatty acids by marine microorganisms while growing in low chloride media by manipulating sodium ion and potassium ion levels. The invention also relates to methods of production of highly unsaturated fatty acids by marine organisms at low pH levels, and includes methods for generation of low pH tolerant strains.

公开(公告)号：US09518102B2

公开(公告)日：2016-12-13

申请号：US14548054

申请日：2014-11-19

Applicant: DSM IP Assets B.V.

Inventor： Kirk E. Apt ,  James Casey Lippmeier ,  David Simpson ,  Jun Wang ,  James P. Wynn ,  Ross Zirkle

IPC: C12N15/62 ,  C07K14/705 ,  C07K14/37 ,  C12N15/80 ,  C12P21/00 ,  C12R1/90 ,  C12N15/79

CPC classification number: C12P21/00 ,  C07K14/37 ,  C07K14/705 ,  C12N9/2431 ,  C12N15/79 ,  C12N15/80 ,  C12P21/005 ,  C12R1/90 ,  C12Y302/01026

Abstract: The present invention relates to recombinant cells and microorganisms of the phylum Labyrinthulomycota and their use in heterologous protein production. Novel promoter, terminator, and signal sequences for efficient production and, optionally, secretion of polypeptides from recombinant host cells and microorganisms are also encompassed by the present invention.

Abstract translation: 本发明涉及神经胶霉病菌门的重组细胞和微生物及其在异源蛋白质生产中的用途。 用于有效生产和任选地从重组宿主细胞和微生物分泌多肽的新型启动子，终止子和信号序列也包括在本发明中。

公开(公告)号：US09382521B2

公开(公告)日：2016-07-05

申请号：US13848070

申请日：2013-03-21

Applicant: DSM IP Assets B.V.

Inventor： James G. Metz ,  Jerry M. Kuner ,  James Casey Lippmeier

IPC: C12N9/10 ,  C12N9/00 ,  C12N15/82 ,  C12P7/64

CPC classification number: C12N9/1029 ,  C12N9/93 ,  C12N15/8247 ,  C12P7/6427 ,  C12P7/6472 ,  Y02P20/52

Abstract: Disclosed are novel acyl-CoA synthetases and novel acyltransferases, nucleic acid molecules encoding the same, recombinant nucleic acid molecules and recombinant host cells comprising such nucleic acid molecules, genetically modified organisms (microorganisms and plants) comprising the same, and methods of making and using the same. Also disclosed are genetically modified organisms (e.g., plants, microorganisms) that have been genetically modified to express a PKS-like system for the production of PUFAs (a PUFA PKS system or PUFA synthase), wherein the organisms have been modified to express an acyl-CoA synthetase, to express an acyl transferase, to delete or inactivate a fatty acid synthase (FAS) expressed by the organism, to reduce competition for malonyl CoA with the PUFA synthase or to increase the level of malonyl CoA in the organism, and in one aspect, to inhibit KASII or KASIII. Additional modifications, and methods to make and use such organisms, in addition to PUFAs and oils obtained from such organisms, are disclosed, alone with various products including such PUFAs and oils.

公开(公告)号：US09012616B2

公开(公告)日：2015-04-21

申请号：US14108794

申请日：2013-12-17

Applicant: DSM IP Assets B.V.

Inventor： Kirk E. Apt ,  James Casey Lippmeier ,  David Simpson ,  Jun Wang ,  James P. Wynn ,  Ross Zirkle

IPC: C07H21/04 ,  C12N1/13 ,  C12P21/02 ,  C07K14/37 ,  C12N15/80 ,  C12P21/00 ,  C12R1/90

CPC classification number: C12P21/00 ,  C07K14/37 ,  C07K14/705 ,  C12N9/2431 ,  C12N15/79 ,  C12N15/80 ,  C12P21/005 ,  C12R1/90 ,  C12Y302/01026

Abstract: The present invention relates to recombinant cells and microorganisms of the phylum Labyrinthulomycota and their use in heterologous protein production. Novel promoter, terminator, and signal sequences for efficient production and, optionally, secretion of polypeptides from recombinant host cells and microorganisms are also encompassed by the present invention.

Abstract translation: 本发明涉及神经胶霉病菌门的重组细胞和微生物及其在异源蛋白质生产中的用途。 用于有效生产和任选地从重组宿主细胞和微生物分泌多肽的新型启动子，终止子和信号序列也包括在本发明中。

公开(公告)号：US20140289904A1

公开(公告)日：2014-09-25

申请号：US13848070

申请日：2013-03-21

Applicant: DSM IP Assets B.V.

Inventor： James G. METZ ,  Jerry M. Kuner ,  James Casey Lippmeier

IPC: C12N9/10 ,  C12N15/82

CPC classification number: C12N9/1029 ,  C12N9/93 ,  C12N15/8247 ,  C12P7/6427 ,  C12P7/6472 ,  Y02P20/52

Abstract: Disclosed are novel acyl-CoA synthetases and novel acyltransferases, nucleic acid molecules encoding the same, recombinant nucleic acid molecules and recombinant host cells comprising such nucleic acid molecules, genetically modified organisms (microorganisms and plants) comprising the same, and methods of making and using the same. Also disclosed are genetically modified organisms (e.g., plants, microorganisms) that have been genetically modified to express a PKS-like system for the production of PUFAs (a PUFA PKS system or PUFA synthase), wherein the organisms have been modified to express an acyl-CoA synthetase, to express an acyl transferase, to delete or inactivate a fatty acid synthase (FAS) expressed by the organism, to reduce competition for malonyl CoA with the PUFA synthase or to increase the level of malonyl CoA in the organism, and in one aspect, to inhibit KASII or KASIII. Additional modifications, and methods to make and use such organisms, in addition to PUFAs and oils obtained from such organisms, are disclosed, alone with various products including such PUFAs and oils.

Abstract translation: 公开了新型酰基辅酶A合成酶和新型酰基转移酶，编码该酰基转移酶的核酸分子，重组核酸分子和包含该核酸分子的重组宿主细胞，包含该核酸分子的转基因生物（微生物和植物），以及制备和使用 一样。 还公开了经遗传修饰以表达用于生产PUFA（PUFA PKS系统或PUFA合酶）的PKS样系统的遗传修饰生物体（例如，植物，微生物），其中生物体被修饰以表达酰基 -CoA合成酶，以表达酰基转移酶，以删除或失活由生物体表达的脂肪酸合酶（FAS），以减少与PUFA合酶的丙二酰辅酶A竞争或增加生物体中丙二酰辅酶A的水平，以及 一方面抑制KASII或KASIII。 除了PUFA和从这些生物获得的油之外，还公开了另外的修饰和制造和使用这些生物体的方法，其中包括各种产品，包括这种PUFA和油。

公开(公告)号：US20130150604A1

公开(公告)日：2013-06-13

申请号：US13762324

申请日：2013-02-07

Applicant: DSM IP Assets B.V.

Inventor： Paul W. BEHRENS ,  John M. Thompson ,  Kirk Apt ,  Joseph W. Pfeifer, III ,  James P. Wynn ,  James Casey Lippmeier ,  Jaouad Fichtali ,  Jon Hansen

IPC: C12P7/64

CPC classification number: C12P7/6427 ,  A61K36/02 ,  C11B1/10 ,  C12P7/6409 ,  C12P7/6472

Abstract: Methods for production of highly unsaturated fatty acids by marine microorganisms, including the heterotrophic marine dinoflagellate Crypthecodinium, using low levels of chloride ion are disclosed. Specifically, methods of increasing production of highly unsaturated fatty acids by marine microorganisms while growing in low chloride media by manipulating sodium ion and potassium ion levels. The invention also relates to methods of production of highly unsaturated fatty acids by marine organisms at low pH levels, and includes methods for generation of low pH tolerant strains.

Abstract translation: 公开了使用低水平氯离子的海洋微生物生产高度不饱和脂肪酸的方法，包括异养海洋鞭毛藻Crypthecodinium。 具体地说，通过操纵钠离子和钾离子水平，在低氯化物培养基中生长时，通过海洋微生物增加高度不饱和脂肪酸的生产的方法。 本发明还涉及在低pH水平下由海洋生物生产高度不饱和脂肪酸的方法，并且包括产生低pH耐受菌株的方法。