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    Mutant protein and methods and materials for making and using it
    11.
    发明授权
    Mutant protein and methods and materials for making and using it 失效
    突变蛋白及其制备和使用的方法和材料

    公开(公告)号：US5874238A

    公开(公告)日：1999-02-23

    申请号：US480270

    申请日：1995-06-07

    申请人： Lawrence A. Potempa ,  Hans H. Liao ,  Becky L. Crump

    发明人： Lawrence A. Potempa ,  Hans H. Liao ,  Becky L. Crump

    IPC分类号： A61K38/00 ,  C07K14/47 ,  C12P21/00 ,  C12N15/12 ,  C12N15/74

    CPC分类号： C07K14/4737 ,  A61K38/00

    摘要： The invention provides a mutant protein which has the same amino acid sequence as an unmutated C-reactive protein (CRP) subunit or an unmutated preCRP, except that at least one amino acid of the unmutated CRP subunit or unmutated preCRP has been deleted, at least one amino acid of the unmutated CRP subunit or unmutated preCRP has been replaced by another amino acid, at least one amino acid has been added to the unmutated CRP subunit or preCRP, or a combination of such changes has been made. The amino acid(s) added, deleted and/or replaced are chosen so that the mutant protein is less likely to form covalently cross-linked aggregates than the unmutated CRP subunit or unmutated preCRP. The mutant protein also exhibits at least one of the biological activities of modified-CRP. The invention also provides DNA molecules coding for the mutant proteins of the invention, vectors for expressing the mutant proteins, host cells which have been transformed so that they can express the mutant proteins, and a method of producing the mutant proteins of the invention comprising culturing the transformed host cells. Finally, the invention provides methods and materials for using the mutant proteins.

    摘要翻译： 本发明提供了与未突变的C-反应蛋白（CRP）亚基或未突变的preCRP具有相同氨基酸序列的突变蛋白，除了未突变的CRP亚基或未突变的preCRP的至少一个氨基酸已被缺失，至少 未突变的CRP亚基或未突变的preCRP的一个氨基酸已经被另一个氨基酸替代，至少一个氨基酸已经添加到未突变的CRP亚基或preCRP中，或者已经进行了这些改变的组合。 选择加入，缺失和/或替换的氨基酸，使得突变蛋白不比未突变的CRP亚基或未突变的preCRP形成共价交联的聚集体。 突变蛋白还表现出修饰CRP的至少一种生物活性。 本发明还提供编码本发明的突变型蛋白质的DNA分子，用于表达突变型蛋白质的载体，已被转化的宿主细胞，使得它们能够表达突变型蛋白质，以及产生本发明的突变型蛋白质的方法，包括培养 转化的宿主细胞。 最后，本发明提供了使用突变蛋白的方法和材料。