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    Methods for detecting nucleic acids using multiple signals
    13.
    发明授权
    Methods for detecting nucleic acids using multiple signals 失效
    使用多种信号检测核酸的方法

    公开(公告)号：US08039216B2

    公开(公告)日：2011-10-18

    申请号：US12845465

    申请日：2010-07-28

    申请人： Maurice Exner ,  Amy Rogers

    发明人： Maurice Exner ,  Amy Rogers

    IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H21/04

    CPC分类号： C12Q1/6818 ,  C12Q2563/173 ,  C12Q2527/107

    摘要： Disclosed is are methods for identifying a nucleic acid in a sample. In one example, the method includes: (a) contacting the nucleic acid in the sample with an oligonucleotide that is specific for the nucleic acid in the sample and that is labeled with at least a first fluorescent dye; (b) contacting the nucleic acid in the sample with a second fluorescent dye that is different from the first fluorescent dye, such that the second fluorescent dye interacts with the nucleic acid; (c) amplifying the nucleic acid if present in the sample; and (d) detecting the nucleic acid if present in the sample by observing fluorescence from the first fluorescent dye after the oligonucleotide hybridizes to the amplified nucleic acid and determining the melting temperature of the amplified nucleic acid by measuring the fluorescence of the second fluorescent dye. The second fluorescent dye may include a fluorescent intercalating agent.

    摘要翻译： 公开了用于鉴定样品中核酸的方法。 在一个实例中，该方法包括：（a）使样品中的核酸与样品中核酸特异的寡核苷酸接触，并用至少第一荧光染料标记; （b）使样品中的核酸与不同于第一荧光染料的第二荧光染料接触，使得第二荧光染料与核酸相互作用; （c）如果存在于样品中则扩增核酸; 以及（d）通过在寡核苷酸与扩增的核酸杂交后通过观察来自第一荧光染料的荧光检测样品中的核酸，并通过测量第二荧光染料的荧光来测定扩增的核酸的解链温度。 第二荧光染料可以包括荧光嵌入剂。

    SYSTEMS AND METHODS FOR DETECTING THE PRESENCE OF A SELECTED VOLUME OF MATERIAL IN A SAMPLE PROCESSING DEVICE
    15.
    发明申请
    SYSTEMS AND METHODS FOR DETECTING THE PRESENCE OF A SELECTED VOLUME OF MATERIAL IN A SAMPLE PROCESSING DEVICE 有权
    用于检测样品处理装置中选择的材料体积的存在的系统和方法

    公开(公告)号：US20120293796A1

    公开(公告)日：2012-11-22

    申请号：US13474903

    申请日：2012-05-18

    申请人： Peter D. Ludowise ,  David A. Whitman ,  Kyle C. Armantrout ,  Maurice Exner ,  Lucien A.E. Jacky ,  Michelle Tabb

    发明人： Peter D. Ludowise ,  David A. Whitman ,  Kyle C. Armantrout ,  Maurice Exner ,  Lucien A.E. Jacky ,  Michelle Tabb

    IPC分类号： G01N21/01

    CPC分类号： C12Q1/686 ,  B01L3/5027 ,  B01L3/502715 ,  B01L2200/0605 ,  B01L2300/087 ,  B01L2400/0409 ,  G01N21/6486 ,  G01N35/025 ,  G01N35/04 ,  G01N2035/0441

    摘要： Systems and methods for processing sample processing devices. The system can include a sample processing device comprising a detection chamber, a motor configured to rotate the sample processing device about an axis of rotation, and an optical module operatively positioned relative to the sample processing device and configured to determine whether a selected volume of material is present in the detection chamber of the sample processing device. The method can include rotating the sample processing device about an axis of rotation, and determining whether a selected volume of material is present in the detection chamber, while rotating the sample processing device. In some embodiments, determining whether a selected volume of material is present can be performed by optically interrogating the detection chamber for an optical property of the material.

    摘要翻译： 用于处理样品处理装置的系统和方法。 该系统可以包括样品处理装置，该样品处理装置包括检测室，被配置为围绕旋转轴线旋转样品处理装置的马达和相对于样品处理装置可操作地定位的光学模块，并配置成确定所选择的材料体积 存在于样品处理装置的检测室中。 该方法可以包括围绕旋转轴旋转样品处理装置，并且在旋转样品处理装置的同时确定所选择的材料体积是否存在于检测室中。 在一些实施例中，确定所选择的材料体积是否存在可以通过光学询问该检测室以获得该材料的光学特性。

    Methods for detecting nucleic acids using multiple signals
    16.
    发明授权
    Methods for detecting nucleic acids using multiple signals 有权
    使用多种信号检测核酸的方法

    公开(公告)号：US07785786B2

    公开(公告)日：2010-08-31

    申请号：US11337805

    申请日：2006-01-23

    申请人： Maurice Exner ,  Amy Rogers

    发明人： Maurice Exner ,  Amy Rogers

    IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H21/04

    CPC分类号： C12Q1/6818 ,  C12Q2563/173 ,  C12Q2527/107

    摘要： Disclosed is are methods for identifying a nucleic acid in a sample. In one example, the method includes: (a) contacting the nucleic acid in the sample with an oligonucleotide that is specific for the nucleic acid in the sample and that is labeled with at least a first fluorescent dye; (b) contacting the nucleic acid in the sample with a second fluorescent dye that is different from the first fluorescent dye, such that the second fluorescent dye interacts with the nucleic acid; (c) amplifying the nucleic acid if present in the sample; and (d) detecting the nucleic acid if present in the sample by observing fluorescence from the first fluorescent dye after the oligonucleotide hybridizes to the amplified nucleic acid and determining the melting temperature of the amplified nucleic acid by measuring the fluorescence of the second fluorescent dye. The second fluorescent dye may include a fluorescent intercalating agent.

    摘要翻译： 公开了用于鉴定样品中核酸的方法。 在一个实例中，该方法包括：（a）使样品中的核酸与样品中核酸特异的寡核苷酸接触，并用至少第一荧光染料标记; （b）使样品中的核酸与不同于第一荧光染料的第二荧光染料接触，使得第二荧光染料与核酸相互作用; （c）如果存在于样品中则扩增核酸; 以及（d）通过在寡核苷酸与扩增的核酸杂交后通过观察来自第一荧光染料的荧光检测样品中的核酸，并通过测量第二荧光染料的荧光来测定扩增的核酸的解链温度。 第二荧光染料可以包括荧光嵌入剂。

    Oligonucleotides and methods for detecting Borrelia burgdorferi
    17.
    发明授权
    Oligonucleotides and methods for detecting Borrelia burgdorferi 有权
    寡核苷酸和检测勃氏疏螺旋体的方法

    公开(公告)号：US06686162B2

    公开(公告)日：2004-02-03

    申请号：US10011340

    申请日：2001-12-04

    申请人： Maurice Exner ,  Hasnah Hamdan ,  Michael Lewinski

    发明人： Maurice Exner ,  Hasnah Hamdan ,  Michael Lewinski

    IPC分类号： C12Q168

    CPC分类号： C12Q1/689 ,  C12Q2600/166 ,  Y02A50/57

    摘要： The present invention provides methods and compositions for determining the presence and/or amount of Borrelia burgdorferi nucleic acids in a test sample related to Lyme disease. In particular, substantially purified oligonucleotide primers and probes are described that can be used for qualitatively and quantitatively detecting Borrelia burgdorferi nucleic acid in a test sample by amplification methods. The present invention also provides primers and probes for generating and detecting control nucleic acid sequences that provide a convenient method for assessing internal quality control of the Borrelia burgdorferi assay.

    摘要翻译： 本发明提供了用于确定与莱姆病相关的测试样品中伯氏疏螺旋体核酸的存在和/或量的方法和组合物。 特别地，描述了基本上纯化的寡核苷酸引物和探针，其可以用于通过扩增方法在测试样品中定性和定量检测勃氏疏螺旋体核酸。 本发明还提供用于产生和检测控制核酸序列的引物和探针，其提供用于评估疏螺旋体凝血酶测定的内部质量控制的方便方法。

    Detection of Mycobacterium tuberculosis complex nucleic acids
    18.
    发明授权
    Detection of Mycobacterium tuberculosis complex nucleic acids 有权
    检测结核分枝杆菌复合核酸

    公开(公告)号：US09145591B1

    公开(公告)日：2015-09-29

    申请号：US13587599

    申请日：2012-08-16

    申请人： Maurice Exner

    发明人： Maurice Exner

    IPC分类号： C07H21/04 ,  C12Q1/68

    CPC分类号： C12Q1/689

    摘要： Disclosed is a method for determining the presence of Mycobacterium tuberculosis complex nucleic acids in a test sample. In particular, regions of the IS6110 preferential locus (ipl) 3′-flanking region of the Mycobacterium tuberculosis complex genome are amplified and detected. In addition, oligonucleotides that can be used as primers to amplify the ipl 3′-flanking region and probe oligonucleotides are described.

    摘要翻译： 公开了一种用于确定测试样品中结核分枝杆菌复合核酸的存在的方法。 特别地，扩增和检测结核分枝杆菌复合物基因组的IS6110优先位点（ipl）3'-侧翼区域。 此外，描述了可用作引物以扩增ipl 3'侧翼区和探针寡核苷酸的寡核苷酸。

    Methods for detecting retroviruses
    19.
    发明授权
    Methods for detecting retroviruses 失效
    检测逆转录病毒的方法

    公开(公告)号：US07566532B1

    公开(公告)日：2009-07-28

    申请号：US11169287

    申请日：2005-06-28

    申请人： Maurice Exner ,  Jamie L. Platt

    发明人： Maurice Exner ,  Jamie L. Platt

    IPC分类号： C12Q1/70 ,  C12P19/34

    CPC分类号： C12Q1/703

    摘要： Disclosed is a method for detecting human retroviral nucleic acids such as human immunodeficiency virus type I (HIV-1) nucleic acid, human T-cell leukemia virus type I (HTLV-I) nucleic acid, and human T-cell leukemia virus type II (HTLV-II) nucleic acid in a sample. In the method, the sample is treated with reverse transcriptase to generate cDNA, and the cDNA is subsequently analyzed to detect HIV-1, HTLV-I, and HTLV-II. The method may include performing PCR and the method may utilize specific primers. In addition, the method may utilize HTLV linkers that facilitate PCR amplification and sequencing. The cDNA may be treated with restriction enzymes before or after PCR amplification to facilitate sequencing and detection of HIV-1, HTLV-I, or HTLV-II nucleic acid.

    摘要翻译： 公开了用于检测人类逆转录病毒核酸如I型（HIV-1）核酸，人类T细胞白血病病毒I型（HTLV-I）核酸和II型人类T细胞白血病病毒 （HTLV-II）核酸。 在该方法中，用逆转录酶处理样品以产生cDNA，随后分析cDNA以检测HIV-1，HTLV-1和HTLV-II。 该方法可以包括进行PCR，并且该方法可以利用特异性引物。 此外，该方法可以利用促进PCR扩增和测序的HTLV接头。 可以在PCR扩增之前或之后用限制酶处理cDNA，以促进HIV-1，HTLV-I或HTLV-II核酸的测序和检测。

    INTERNAL POSITIVE CONTROL FOR NUCLEIC ACID ASSAYS
    20.
    发明申请
    INTERNAL POSITIVE CONTROL FOR NUCLEIC ACID ASSAYS 审中-公开
    内部酸性测定的内部控制

    公开(公告)号：US20090181366A1

    公开(公告)日：2009-07-16

    申请号：US11830759

    申请日：2007-07-30

    申请人： Edgar B. Ong ,  Maurice Exner ,  Michael A. Lewinski

    发明人： Edgar B. Ong ,  Maurice Exner ,  Michael A. Lewinski

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6851 ,  C12Q2545/101 ,  C12Q2527/127

    摘要： Compositions and methods for detecting a non-specific nucleic acid amplification inhibitor in a reaction are disclosed. An internal positive control (IPC) may be included in samples to be tested for target nucleic acids as a means to monitor non-specific inhibition of nucleic acid amplification and provide confidence in negative results obtained in target-specific assays. Provided herein are an IPC polynucleotide, IPC control primers, and IPC probes. Also provided are methods of using an IPC polynucleotide, primers, and probes to detect a non-specific nucleic acid amplification inhibitor.

    摘要翻译： 公开了用于在反应中检测非特异性核酸扩增抑制剂的组合物和方法。 可以在待测试的靶核酸样品中包括内部阳性对照（IPC），作为监测核酸扩增的非特异性抑制的手段，并为靶特异性测定中获得的阴性结果提供置信度。 本文提供了IPC多核苷酸，IPC对照引物和IPC探针。 还提供了使用IPC多核苷酸，引物和探针来检测非特异性核酸扩增抑制剂的方法。