公开(公告)号：US20160208322A1

公开(公告)日：2016-07-21

申请号：US14723872

申请日：2015-05-28

Applicant: Fluidigm Corporation

Inventor： Megan Anderson ,  Peilin Chen ,  Brian Fowler ,  Robert C. Jones ,  Fiona Kaper ,  Ronald Lebofsky ,  Andrew May

IPC: C12Q1/68 ,  C12N15/10

CPC classification number: C12Q1/6855 ,  C12N15/10 ,  C12N15/1065 ,  C12N15/65 ,  C12N15/66 ,  C12Q1/686 ,  C12Q1/6874

Abstract: Described herein are methods useful for incorporating one or more adaptors and/or nucleotide tag(s) and/or barcode nucleotide sequence(s) one, or typically more, target nucleotide sequences. In particular embodiments, nucleic acid fragments having adaptors, e.g., suitable for use in high-throughput DNA sequencing are generated. In other embodiments, information about a reaction mixture is encoded into a reaction product. Also described herein are methods and kits useful for amplifying one or more target nucleic acids in preparation for applications such as bidirectional nucleic acid sequencing. In particular embodiments, methods of the invention entail additionally carrying out bidirectional DNA sequencing. Also described herein are methods for encoding and detecting and/or quantifying alleles by primer extension.

公开(公告)号：US20140154787A1

公开(公告)日：2014-06-05

申请号：US13915476

申请日：2013-06-11

Applicant: Fluidigm Corporation

Inventor： Gang Sun ,  Ramesh Ramakrishnan ,  Robert C. Jones

IPC: C12Q1/68

CPC classification number: C12Q1/686 ,  C12Q2565/629 ,  C12Q2565/501 ,  C12Q2545/114

Abstract: A method of adjusting amplification curves in a PCR experiment includes receiving a plurality of amplification curves for a sample and computing a first parameter for each of the plurality of amplification curves. The method also includes computing a second parameter for each of the plurality of amplification curves and computing a third parameter using at least a portion of the first or second parameters. The method further includes computing an offset for each of the plurality of amplification curves. The offset is a function of the first parameter and the third parameter. Moreover, the method includes adjusting at least one of the plurality of amplification curves by subtracting the offset.

Abstract translation: 在PCR实验中调整扩增曲线的方法包括接收样品的多个放大曲线并计算多个放大曲线中的每一个的第一参数。 该方法还包括为多个放大曲线中的每一个计算第二参数，并使用第一或第二参数的至少一部分来计算第三参数。 该方法还包括计算多个放大曲线中的每一个的偏移量。 偏移量是第一个参数和第三个参数的函数。 此外，该方法包括通过减去偏移来调整多个放大曲线中的至少一个。

公开(公告)号：US20140045729A1

公开(公告)日：2014-02-13

申请号：US13863973

申请日：2013-04-16

Applicant: Fluidigm Corporation

Inventor： Robert C. Jones ,  Jing Wang ,  Andrew May ,  David Cohen

IPC: B01L3/00

CPC classification number: B01J19/00 ,  B01F5/0085 ,  B01F5/0602 ,  B01F13/0069 ,  B01J2219/00283 ,  B01J2219/00286 ,  B01J2219/00315 ,  B01J2219/00317 ,  B01J2219/00351 ,  B01J2219/00355 ,  B01J2219/00389 ,  B01J2219/00418 ,  B01L3/502715 ,  B01L3/502738 ,  B01L3/5085 ,  B01L7/52 ,  B01L2200/16 ,  B01L2300/0654 ,  B01L2300/0829 ,  B01L2300/123 ,  B01L2300/1805 ,  B01L2400/0481 ,  B01L2400/0605 ,  B01L2400/0655

Abstract: An SBS-formatted microfluidic device where the geometry of the plate defines an array of interrogation areas, and where each interrogation area encompasses at least one reaction site.

Abstract translation: SBS格式的微流体装置，其中板的几何形状限定了询问区域阵列，并且其中每个询问区域包含至少一个反应位点。