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    Methods and kits for extracting nucleic acids from paraffin embedded samples
    2.
    发明授权
    Methods and kits for extracting nucleic acids from paraffin embedded samples 有权

    公开(公告)号：US11078476B2

    公开(公告)日：2021-08-03

    申请号：US16105711

    申请日：2018-08-20

    Applicant: Fluidigm Corporation

    Inventor： Jian Qin ,  Peilin Chen ,  Ramesh Ramakrishnan

    IPC: C12N15/10

    Abstract: This invention provides a technology for isolating nucleic acids from wax-embedded samples that is superior to the current state of the art. Standard protocols with this objective typically comprise dissolving the wax-embedded sample in an organic solvent, extracting nucleic acids from the organic solvent into an aqueous buffer, and isolating the nucleic acids from the aqueous buffer. The technology described here includes using hexadecane as the solvent to dissolve the sample, precipitating and washing the extracted nucleic acids, and dissolving the nucleic acids in a lysis buffer that includes NP40 and SDS. By implementing the reagents and techniques described in this disclosure, the user can obtain a product that has better yield, less degradation, and contains more unique mRNA transcripts for subsequent sequencing and analysis.

    METHODS AND KITS FOR EXTRACTING NUCLEIC ACIDS FROM PARAFFIN EMBEDDED SAMPLES
    3.
    发明申请
    METHODS AND KITS FOR EXTRACTING NUCLEIC ACIDS FROM PARAFFIN EMBEDDED SAMPLES 审中-公开

    公开(公告)号：US20190055540A1

    公开(公告)日：2019-02-21

    申请号：US16105711

    申请日：2018-08-20

    Applicant: Fluidigm Corporation

    Inventor： Jian Qin ,  Peilin Chen ,  Ramesh Ramakrishnan

    IPC: C12N15/10

    Abstract: This invention provides a technology for isolating nucleic acids from wax-embedded samples that is superior to the current state of the art. Standard protocols with this objective typically comprise dissolving the wax-embedded sample in an organic solvent, extracting nucleic acids from the organic solvent into an aqueous buffer, and isolating the nucleic acids from the aqueous buffer. The technology described here includes using hexadecane as the solvent to dissolve the sample, precipitating and washing the extracted nucleic acids, and dissolving the nucleic acids in a lysis buffer that includes NP40 and SDS. By implementing the reagents and techniques described in this disclosure, the user can obtain a product that has better yield, less degradation, and contains more unique mRNA transcripts for subsequent sequencing and analysis.

    COPY NUMBER VARIATION DETERMINATION, METHODS AND SYSTEMS
    7.
    发明申请
    COPY NUMBER VARIATION DETERMINATION, METHODS AND SYSTEMS 审中-公开

    公开(公告)号：US20130260381A1

    公开(公告)日：2013-10-03

    申请号：US13902667

    申请日：2013-05-24

    Applicant: Fluidigm Corporation

    Inventor： Ramesh Ramakrishnan

    IPC: C12Q1/68

    CPC classification number: C12Q1/6869 ,  C12Q1/6851 ,  C12Q2545/101 ,  C12Q2527/143

    Abstract: The present invention methods and systems for determining copy number variation of a target polynucleotide in a genome of a subject including amplification based techniques. Methods can include pre-amplification of the sample followed by distribution of sample and a plurality of reaction volumes, quantitative detection of a target polynucleotide and a reference polynucleotide, and analysis so as to determine the relative copy number of the target polynucleotide sequence in the genome of the subject.

    METHOD AND APPARATUS FOR BIOLOGICAL SAMPLE ANALYSIS
    9.
    发明申请
    METHOD AND APPARATUS FOR BIOLOGICAL SAMPLE ANALYSIS 审中-公开
    生物样品分析方法与装置

    公开(公告)号：US20140154787A1

    公开(公告)日：2014-06-05

    申请号：US13915476

    申请日：2013-06-11

    Applicant: Fluidigm Corporation

    Inventor： Gang Sun ,  Ramesh Ramakrishnan ,  Robert C. Jones

    IPC: C12Q1/68

    CPC classification number: C12Q1/686 ,  C12Q2565/629 ,  C12Q2565/501 ,  C12Q2545/114

    Abstract: A method of adjusting amplification curves in a PCR experiment includes receiving a plurality of amplification curves for a sample and computing a first parameter for each of the plurality of amplification curves. The method also includes computing a second parameter for each of the plurality of amplification curves and computing a third parameter using at least a portion of the first or second parameters. The method further includes computing an offset for each of the plurality of amplification curves. The offset is a function of the first parameter and the third parameter. Moreover, the method includes adjusting at least one of the plurality of amplification curves by subtracting the offset.

    Abstract translation: 在PCR实验中调整扩增曲线的方法包括接收样品的多个放大曲线并计算多个放大曲线中的每一个的第一参数。 该方法还包括为多个放大曲线中的每一个计算第二参数，并使用第一或第二参数的至少一部分来计算第三参数。 该方法还包括计算多个放大曲线中的每一个的偏移量。 偏移量是第一个参数和第三个参数的函数。 此外，该方法包括通过减去偏移来调整多个放大曲线中的至少一个。

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