-
公开(公告)号：US12097501B2
公开(公告)日：2024-09-24
申请号：US17181966
申请日：2021-02-22
Applicant: Fluidigm Corporation
Inventor： Michael L. Phelan , Christopher J. Kubu , Brian Fowler , Gang Sun , Nikita Patel , Naveen Ramalingam
CPC classification number: B01L7/52 , B01L3/502715 , B01L3/527 , C12Q1/686 , B01L2200/027 , B01L2200/10 , B01L2300/0654 , B01L2300/0672 , B01L2300/0816 , B01L2300/0867 , B01L2300/0887 , B01L2300/1822 , B01L2400/0421 , B01L2400/0481 , B01L2400/0487 , B01L2400/0655
Abstract: Described herein are methods, kits and systems for sample enrichment, multi-step library preparation, sample normalization, detection of sample biomolecules and combinations thereof. Enrichment and multi-step library preparation is described in the context of microfluidic workflows. Sample barcoding methods and kits are described for increasing sample throughput while reducing background in negative samples. Integrated microfluidic devices comprising sample processing unit cells coupled to an array of reaction sites are provided for integrated workflows.
-
公开(公告)号：US20160114327A1
公开(公告)日：2016-04-28
申请号：US14864491
申请日：2015-09-24
Applicant: Fluidigm Corporation
Inventor： Jake Kimball , Brandon Ripley , Gang Sun , Dominique Toppani , Myo Thu Maung
IPC: B01L7/00
CPC classification number: B01L7/52 , B01L3/5027 , B01L2200/025 , B01L2200/147 , B01L2300/0819 , B01L2300/0893 , B01L2300/1805 , B01L2300/1822 , C12P19/34 , C12Q3/00 , G01N21/6408 , G01N21/6428 , G01N21/6456 , G01N21/76
Abstract: A thermal cycler for a microfluidic device includes a controller operable to provide a series of electrical signals, a heat sink, and a heating element in thermal communication with the heat sink and operable to receive the series of electrical signals from the controller. The thermal cycler also includes a thermal chuck in thermal communication with the heating element. The thermal chuck comprises a heating surface operable to make thermal contact with the microfluidic device. The heating surface is characterized by a temperature ramp rate between 2.5 degrees Celsius per second and 5.5 degrees Celsius per second and a temperature difference between a first portion of the heating surface supporting a first portion of the microfluidic device and a second portion of the heating surface supporting a second portion of the microfluidic device is less than 0.25° C.
Abstract translation: 用于微流体装置的热循环仪包括控制器，其可操作以提供与散热器热连通的一系列电信号，散热器和加热元件，并可操作以从控制器接收一系列电信号。 热循环仪还包括与加热元件热连通的热卡盘。 热卡盘包括可操作以与微流体装置进行热接触的加热表面。 加热表面的特征在于每秒2.5摄氏度和5.5摄氏度/秒之间的温度升高速率，并且支撑微流体装置的第一部分的加热表面的第一部分与加热表面的第二部分之间的温度差 支撑微流体装置的第二部分小于0.25℃
-
公开(公告)号：US20130266204A1
公开(公告)日：2013-10-10
申请号：US13755754
申请日：2013-01-31
Applicant: Fluidigm Corporation
Inventor： Emerson Chueng Quan , Colin Jon Taylor , Michael Lee , Christopher Ceasar , Greg Harris , Gang Sun
IPC: B01L3/00
CPC classification number: G01N33/00 , B01L3/5025 , B01L3/502707 , B01L2200/025 , B01L2200/12 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2300/087 , B01L2300/0887 , B01L2400/0481 , B01L2400/0655 , Y10T436/11
Abstract: A biological substrate, e.g., microfluidic chip. The substrate includes a rigid substrate material, which has a surface region capable of acting as a handle substrate. The substrate also has a deformable fluid layer coupled to the surface region. One or more well regions are formed in a first portion of the deformable fluid layer and are capable of holding a fluid therein. The one or more channel regions are formed in a second portion of the deformable fluid layer and are coupled to one or more of the well regions. An active region is formed in the deformable fluid layer. At least three fiducial markings are formed within the non-active region and disposed in a spatial manner associated with at least one of the well regions. A control layer is coupled to the fluid layer.
-
公开(公告)号：US20190217302A1
公开(公告)日：2019-07-18
申请号：US16245537
申请日：2019-01-11
Applicant: Fluidigm Corporation
Inventor： Jake Kimball , Brandon Ripley , Gang Sun , Dominique Toppani , Myo Thu Maung
CPC classification number: B01L7/52 , B01L3/5027 , B01L2200/025 , B01L2200/147 , B01L2300/0819 , B01L2300/0893 , B01L2300/1805 , B01L2300/1822 , C12P19/34 , C12Q3/00 , G01N21/6408 , G01N21/6428 , G01N21/6456 , G01N21/76
Abstract: A thermal cycler for a microfluidic device includes a controller operable to provide a series of electrical signals, a heat sink, and a heating element in thermal communication with the heat sink and operable to receive the series of electrical signals from the controller. The thermal cycler also includes a thermal chuck in thermal communication with the heating element. The thermal chuck comprises a heating surface operable to make thermal contact with the microfluidic device. The heating surface is characterized by a temperature ramp rate between 2.5 degrees Celsius per second and 5.5 degrees Celsius per second and a temperature difference between a first portion of the heating surface supporting a first portion of the microfluidic device and a second portion of the heating surface supporting a second portion of the microfluidic device is less than 0.25° C.
-
公开(公告)号：US08849037B2
公开(公告)日：2014-09-30
申请号：US14071423
申请日：2013-11-04
Applicant: Fluidigm Corporation
Inventor： Simant Dube , Gang Sun , Lian-She Zhao
CPC classification number: G01N21/64 , G06T7/0012 , G06T7/74 , G06T2207/10064 , G06T2207/30004
Abstract: A method of processing data associated with fluorescent emissions from a microfluidic device. The method includes performing an auto-focus process associated with a first image of the microfluidic device and performing an auto-exposure process associated with the first image of the microfluidic device. The method also includes capturing a plurality of images of the microfluidic device. The plurality of images are associated with a plurality of thermal cycles. The method further includes performing image analysis of the plurality of captured images to determine a series of optical intensities and performing data analysis of the series of optical intensities to provide a series of change in threshold values.
Abstract translation: 处理与微流体装置的荧光发射有关的数据的方法。 该方法包括执行与微流体装置的第一图像相关联的自动聚焦处理，并且执行与微流体装置的第一图像相关联的自动曝光处理。 该方法还包括捕获微流体装置的多个图像。 多个图像与多个热循环相关联。 该方法还包括执行多个拍摄图像的图像分析以确定一系列光强度并执行该系列光强度的数据分析以提供一系列阈值变化。
-
Patent Agency Ranking
公开(公告)号：US08808640B2
公开(公告)日：2014-08-19
申请号：US13755754
申请日：2013-01-31
Applicant: Fluidigm Corporation
Inventor： Emerson Chueng Quan , Colin Jon Taylor , Michael Lee , Christopher Ceasar , Greg Harris , Gang Sun
IPC: G01N33/00
CPC classification number: G01N33/00 , B01L3/5025 , B01L3/502707 , B01L2200/025 , B01L2200/12 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2300/087 , B01L2300/0887 , B01L2400/0481 , B01L2400/0655 , Y10T436/11
Abstract: A biological substrate, e.g., microfluidic chip. The substrate includes a rigid substrate material, which has a surface region capable of acting as a handle substrate. The substrate also has a deformable fluid layer coupled to the surface region. One or more well regions are formed in a first portion of the deformable fluid layer and are capable of holding a fluid therein. The one or more channel regions are formed in a second portion of the deformable fluid layer and are coupled to one or more of the well regions. An active region is formed in the deformable fluid layer. At least three fiducial markings are formed within the non-active region and disposed in a spatial manner associated with at least one of the well regions. A control layer is coupled to the fluid layer.
Abstract translation: 生物底物，例如微流体芯片。 衬底包括刚性衬底材料，其具有能够用作手柄衬底的表面区域。 衬底还具有耦合到表面区域的可变形流体层。 一个或多个阱区域形成在可变形流体层的第一部分中并且能够在其中保持流体。 一个或多个通道区域形成在可变形流体层的第二部分中并且耦合到一个或多个阱区域。 在可变形流体层中形成有源区。 在非有源区域内形成至少三个基准标记，并以与至少一个阱区域相关联的空间方式设置。 控制层耦合到流体层。
-
公开(公告)号：US20220017953A1
公开(公告)日：2022-01-20
申请号：US17333792
申请日：2021-05-28
Applicant: Fluidigm Corporation
Inventor： Michael L. Phelan , Christopher J. Kubu , Brian Fowler , Gang Sun , Nikita Patel , Naveen Ramalingam
IPC: C12Q1/6851 , C12Q1/6806 , C12Q1/70
Abstract: Described herein are methods, kits and systems for sample enrichment, multi-step library preparation, sample normalization, detection of sample biomolecules and combinations thereof. Enrichment and multi-step library preparation is described in the context of microfluidic workflows. Sample barcoding methods and kits are described for increasing sample throughput while reducing background in negative samples. Integrated microfluidic devices comprising sample processing unit cells coupled to an array of reaction sites are provided for integrated workflows.
-
公开(公告)号：US20210268508A1
公开(公告)日：2021-09-02
申请号：US17181966
申请日：2021-02-22
Applicant: Fluidigm Corporation
Inventor： Michael L. Phelan , Christopher J. Kubu , Brian Fowler , Gang Sun , Nikita Patel , Naveen Ramalingam
Abstract: Described herein are methods, kits and systems for sample enrichment, multi-step library preparation, sample normalization, detection of sample biomolecules and combinations thereof. Enrichment and multi-step library preparation is described in the context of microfluidic workflows. Sample barcoding methods and kits are described for increasing sample throughput while reducing background in negative samples. Integrated microfluidic devices comprising sample processing unit cells coupled to an array of reaction sites are provided for integrated workflows.
-
公开(公告)号：US10226770B2
公开(公告)日：2019-03-12
申请号：US14864491
申请日：2015-09-24
Applicant: Fluidigm Corporation
Inventor： Jake Kimball , Brandon Ripley , Gang Sun , Dominique Toppani , Myo Thu Maung
Abstract: A thermal cycler for a microfluidic device includes a controller operable to provide a series of electrical signals, a heat sink, and a heating element in thermal communication with the heat sink and operable to receive the series of electrical signals from the controller. The thermal cycler also includes a thermal chuck in thermal communication with the heating element. The thermal chuck comprises a heating surface operable to make thermal contact with the microfluidic device. The heating surface is characterized by a temperature ramp rate between 2.5 degrees Celsius per second and 5.5 degrees Celsius per second and a temperature difference between a first portion of the heating surface supporting a first portion of the microfluidic device and a second portion of the heating surface supporting a second portion of the microfluidic device is less than 0.25° C.
-
10.发明申请ANALYSIS ENGINE AND DATABASE FOR MANIPULATING PARAMETERS FOR FLUIDIC SYSTEMS ON A CHIP 审中-公开用于流动系统的芯片操作参数的分析发动机和数据库公开(公告)号：US20160238623A1
公开(公告)日：2016-08-18
申请号：US15136610
申请日：2016-04-22
Applicant: Fluidigm Corporation
Inventor： Gang Sun , Greg Harris , Andy May , Kyle Self , Kevin Farrell , Paul Wyatt
CPC classification number: G01N35/00029 , B01L3/5027 , B01L3/502715 , B01L2200/10 , B01L2300/06 , B01L2300/0654 , B01L2300/0829 , C12Q1/686 , G01N15/1475 , G01N21/6428 , G01N35/00732 , G01N35/00871 , G01N35/0092 , G01N35/0099 , G01N35/1065 , G01N2035/00148 , G01N2035/00237 , G01N2035/00752 , G01N2035/0091 , G01N2035/1034 , G01N2201/025 , G01N2201/0446 , Y10S901/01 , Y10T436/11
Abstract: A system includes a platform including one or more workstations and a microfluidic input device coupled to the one or more workstations. The microfluidic input device is adapted to receive a microfluidic device from a user. The system also includes a robotic device comprising a robotic arm and disposed on the platform. The robotic arm is capable of accessing the one or more workstations and is configured to transfer a plurality of sample solutions from a first spatial location to the microfluidic device when coupled to the microfluidic input device. The system further includes a multi-pixel image capturing device optically coupled to the microfluidic device and an image processing device operably coupled to the multi-pixel image capturing device. The multi-pixel image capturing device is adapted to capture a plurality of multi-pixel images. The image processing device is configured to receive the plurality of multi-pixel images.
Abstract translation: 系统包括包括一个或多个工作站的平台和耦合到该一个或多个工作站的微流体输入设备。 微流体输入装置适于从用户接收微流体装置。 该系统还包括机器人装置，其包括机器臂并且设置在平台上。 机器臂能够访问一个或多个工作站，并且被配置为当耦合到微流体输入装置时将多个样品溶液从第一空间位置传送到微流体装置。 该系统还包括光学耦合到微流体装置的多像素图像捕获装置和可操作地耦合到多像素图像捕获装置的图像处理装置。 多像素图像捕获装置适于捕获多个多像素图像。 图像处理装置被配置为接收多个多像素图像。
-
-
-
-
-
-
-
-
-
Search Results
13
records
(took 0.019 seconds)
