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    ISOTHERMAL AMPLIFICATION OF NUCLEIC ACIDS WITHIN A POROUS MATRIX
    11.
    发明申请
    ISOTHERMAL AMPLIFICATION OF NUCLEIC ACIDS WITHIN A POROUS MATRIX 审中-公开
    在多孔矩阵中的核酸的同位素放大

    公开(公告)号：US20150167065A1

    公开(公告)日：2015-06-18

    申请号：US14106264

    申请日：2013-12-13

    Applicant: General Electric Company

    Inventor： John Richard Nelson ,  David Roger Moore ,  Bing Li ,  Robert Scott Duthie ,  Patrick McCoy Spooner

    IPC: C12Q1/68

    CPC classification number: C12Q1/6844 ,  C12Q1/6853 ,  C12Q2521/307 ,  C12Q2531/119

    Abstract: Provided herein are methods for amplification a target dsDNA that is impregnated within a porous matrix using endonuclease-assisted DNA amplification. The amplicons may be subsequent detected within the porous matrix or may be eluted out of the porous matrix. Methods for extracting a genetic material from a biological sample using endonuclease-assisted DNA amplification within a porous matrix are also provided.

    Abstract translation: 本文提供了使用内切核酸酶辅助的DNA扩增来扩增浸渍在多孔基质内的靶dsDNA的方法。 可以在多孔基质内随后检测扩增子，或者可以从多孔基质中洗脱。 还提供了在多孔基质内使用内切核酸酶辅助的DNA扩增从生物样品中提取遗传物质的方法。

    SUBSTRATES AND ASSOCIATED METHODS FOR ELUTION OF NUCLEIC ACIDS
    12.
    发明申请
    SUBSTRATES AND ASSOCIATED METHODS FOR ELUTION OF NUCLEIC ACIDS 有权
    用于提取核酸的基质和相关方法

    公开(公告)号：US20150118683A1

    公开(公告)日：2015-04-30

    申请号：US14068532

    申请日：2013-10-31

    Applicant: General Electric Company

    Inventor： Bing Li ,  Gregory Andrew Grossmann ,  David Roger Moore

    IPC: C12Q1/68

    CPC classification number: C12N15/1006

    Abstract: A solid substrate for biological sample storage under dry-state and elution of biomolecules is provided. The dry, solid substrate comprises a surface modified with a plurality of hydrophilic groups; and the substrate is comprised of one or more protein denaturing agents impregnated therein under a substantially dry state. A method for elution of biomolecules from biological samples is also provided. The compositions disclosed herein provide for enhanced elution and recovery of biomolecules, such as nucleic acids, from the sample. The sample is disposed on a substrate, dried to a substantially dry state; eluted from the biological sample dried on the substrate by rehydrating the substrate in an elution buffer.

    Abstract translation: 提供了一种在干态下生物样品储存和生物分子洗脱的固体底物。 干燥的固体基质包括用多个亲水基团改性的表面; 并且底物由在基本干燥状态下浸渍的一种或多种蛋白质变性剂组成。 还提供了用于从生物样品中洗脱生物分子的方法。 本文公开的组合物提供来自样品的生物分子（例如核酸）的增强的洗脱和回收。 将样品置于基材上，干燥至基本干燥状态; 通过在洗脱缓冲液中再水化底物，从在底物上干燥的生物样品中洗脱。

    Substrates and associated methods for elution of nucleic acids
    14.
    发明授权
    Substrates and associated methods for elution of nucleic acids 有权

    公开(公告)号：US09719082B2

    公开(公告)日：2017-08-01

    申请号：US14068532

    申请日：2013-10-31

    Applicant: General Electric Company

    Inventor： Bing Li ,  Gregory Andrew Grossmann ,  David Roger Moore

    IPC: C07H21/00 ,  C12N15/10

    CPC classification number: C12N15/1006

    Abstract: A solid substrate for biological sample storage under dry-state and elution of biomolecules is provided. The dry, solid substrate comprises a surface modified with a plurality of hydrophilic groups; and the substrate is comprised of one or more protein denaturing agents impregnated therein under a substantially dry state. A method for elution of biomolecules from biological samples is also provided. The compositions disclosed herein provide for enhanced elution and recovery of biomolecules, such as nucleic acids, from the sample. The sample is disposed on a substrate, dried to a substantially dry state; eluted from the biological sample dried on the substrate by rehydrating the substrate in an elution buffer.

    METHODS FOR CAPTURING NUCLEIC ACIDS
    16.
    发明申请
    METHODS FOR CAPTURING NUCLEIC ACIDS 审中-公开
    捕获核酸的方法

    公开(公告)号：US20160002622A1

    公开(公告)日：2016-01-07

    申请号：US14566865

    申请日：2014-12-11

    Applicant: General Electric Company

    Inventor： John Richard Nelson ,  Bing Li

    IPC: C12N15/10

    CPC classification number: C12N15/1006

    Abstract: A method is provided herein, wherein the method of capturing a target nucleic acid, comprises applying a nucleic acid capture probe to a capture zone of a needs definition, wherein the nucleic acid capture probe having a first molecular weight comprises at least a sequence that is complimentary to at least a portion of the target nucleic acid sequence and the nucleic acid capture probe is substantially immobilized at the capture zone of the substrate. The method further comprises applying a sample comprising the target nucleic acid having a second molecular weight to a sample application zone of the substrate; wherein the sample comprising the target nucleic acid flows across a length of the substrate from the sample application zone to the capture zone by lateral flow, and the target nucleic acid is captured by the nucleic acid capture probes by hybridization to the capture zone.

    Abstract translation: 本文提供了一种捕获靶核酸的方法，包括将核酸捕获探针应用于需要定义的捕获区，其中具有第一分子量的核酸捕获探针包含至少一个序列， 与靶核酸序列的至少一部分互补，并且核酸捕获探针基本上固定在基底的捕获区。 所述方法还包括将包含具有第二分子量的靶核酸的样品施加到所述基底的样品施用区域; 其中包含靶核酸的样品通过横向流从样品施用区域流过基底的一段长度到捕获区，并且核酸捕获探针通过与捕获区杂交捕获靶核酸。

    Methods and compositions for extraction and storage of nucleic acids
    17.
    发明授权
    Methods and compositions for extraction and storage of nucleic acids 有权
    用于提取和储存核酸的方法和组合物

    公开(公告)号：US09040679B2

    公开(公告)日：2015-05-26

    申请号：US13968497

    申请日：2013-08-16

    Applicant: General Electric Company

    Inventor： Erik Leeming Kvam ,  Bing Li ,  Brian Christopher Bales

    IPC: C12N15/10 ,  B01J20/24

    CPC classification number: B01J20/24 ,  C12N15/1003 ,  C12N15/1006

    Abstract: A solid matrix for the extraction, stabilization, and storage of nucleic acids is provided. At least one protein denaturant, and at least one acid or acid-titrated buffer reagent are impregnated in a dry state therein the matrix; and the matrix is configured to provide an acidic pH on hydration. The matrix is configured to extract nucleic acids from a sample and stabilize the extracted nucleic acids, particularly RNA, in a dry format under ambient conditions for a prolonged period of time. Methods for collecting and recovering the nucleic acids stored in the dry solid matrix are also described.

    Abstract translation: 提供了用于提取，稳定和储存核酸的固体基质。 至少一种蛋白质变性剂和至少一种酸或酸滴定的缓冲剂在基质中以干燥状态浸渍; 并且所述基质配置成在水合时提供酸性pH。 该基质构造成从样品中提取核酸，并在环境条件下长时间地以干燥形式稳定所提取的核酸，特别是RNA。 还描述了收集和回收存储在干燥固体基质中的核酸的方法。

    Methods and compositions for extraction and storage of nucleic acids
    18.
    发明授权
    Methods and compositions for extraction and storage of nucleic acids 有权

    公开(公告)号：US08981077B2

    公开(公告)日：2015-03-17

    申请号：US13968497

    申请日：2013-08-16

    Applicant: General Electric Company

    Inventor： Erik Leeming Kvam ,  Bing Li ,  Brian Christopher Bales

    IPC: C12N15/10 ,  B01J20/24

    Abstract: A solid matrix for the extraction, stabilization, and storage of nucleic acids is provided. At least one protein denaturant, and at least one acid or acid-titrated buffer reagent are impregnated in a dry state therein the matrix; and the matrix is configured to provide an acidic pH on hydration. The matrix is configured to extract nucleic acids from a sample and stabilize the extracted nucleic acids, particularly RNA, in a dry format under ambient conditions for a prolonged period of time. Methods for collecting and recovering the nucleic acids stored in the dry solid matrix are also described.

    METHODS AND COMPOSITIONS FOR EXTRACTION AND STORAGE OF NUCLEIC ACIDS
    19.
    发明申请
    METHODS AND COMPOSITIONS FOR EXTRACTION AND STORAGE OF NUCLEIC ACIDS 有权
    提取和储存核酸的方法和组合物

    公开(公告)号：US20130338351A1

    公开(公告)日：2013-12-19

    申请号：US13968497

    申请日：2013-08-16

    Applicant: General Electric Company

    Inventor： Erik Leeming Kvam ,  Bing Li ,  Brian Christopher Bales

    IPC: B01J20/24 ,  C12N15/10

    CPC classification number: B01J20/24 ,  C12N15/1003 ,  C12N15/1006

    Abstract: A solid matrix for the extraction, stabilization, and storage of nucleic acids is provided. At least one protein denaturant, and at least one acid or acid-titrated buffer reagent are impregnated in a dry state therein the matrix; and the matrix is configured to provide an acidic pH on hydration. The matrix is configured to extract nucleic acids from a sample and stabilize the extracted nucleic acids, particularly RNA, in a dry format under ambient conditions for a prolonged period of time. Methods for collecting and recovering the nucleic acids stored in the dry solid matrix are also described.

    Abstract translation: 提供了用于提取，稳定和储存核酸的固体基质。 至少一种蛋白质变性剂和至少一种酸或酸滴定的缓冲剂在基质中以干燥状态浸渍; 并且所述基质配置成在水合时提供酸性pH。 该基质构造成从样品中提取核酸，并在环境条件下长时间地以干燥形式稳定所提取的核酸，特别是RNA。 还描述了收集和回收存储在干燥固体基质中的核酸的方法。

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