公开(公告)号：US20140134616A1

公开(公告)日：2014-05-15

申请号：US14073445

申请日：2013-11-06

Applicant: Genia Technologies, Inc.

Inventor： Randall Davis ,  Roger Chen ,  Arkadiusz Bibillo ,  Daniel Korenblum ,  Michael Dorwart

IPC: C12Q1/68

CPC classification number: C12Q1/6869 ,  G01N27/44717 ,  G01N27/44791 ,  G01N33/48721 ,  C12Q2527/113 ,  C12Q2535/122 ,  C12Q2563/113 ,  C12Q2565/631

Abstract: This disclosure provides chips, systems and methods for sequencing a nucleic acid sample. Tagged nucleotides are provided into a reaction chamber comprising a nanopore in a membrane. An individual tagged nucleotide of the tagged nucleotides can contain a tag coupled to a nucleotide, which tag is detectable with the aid of the nanopore. Next, an individual tagged nucleotide of the tagged nucleotides can be incorporated into a growing strand complementary to a single stranded nucleic acid molecule derived from the nucleic acid sample. With the aid of the nanopore, a tag associated with the individual tagged nucleotide can be detected upon incorporation of the individual tagged nucleotide. The tag can be detected with the aid of the nanopore when the tag is released from the nucleotide.

Abstract translation: 本公开提供了用于对核酸样品进行测序的芯片，系统和方法。 将标记的核苷酸提供到包含膜中的纳米孔的反应室中。 标记的核苷酸的单个标记的核苷酸可以含有与核苷酸偶联的标签，该标签借助于纳米孔可检测。 接下来，标记的核苷酸的单个标记的核苷酸可以掺入与衍生自核酸样品的单链核酸分子互补的生长链中。 借助纳米孔，可以在掺入单个标记的核苷酸时检测与单个标记的核苷酸相关的标签。 当标签从核苷酸释放时，可以借助纳米孔检测标签。

公开(公告)号：US10273536B2

公开(公告)日：2019-04-30

申请号：US15066117

申请日：2016-03-10

Applicant: Genia Technologies, Inc.

Inventor： Randall W. Davis ,  Edward Shian Liu ,  Eric Takeshi Harada ,  Anne Aguirre ,  Andrew Trans ,  James Pollard ,  Cynthia Cech ,  Hui Tian ,  Robert Yuan ,  John Foster ,  Roger Chen

IPC: G01N27/327 ,  C12Q1/6869 ,  C12M1/34 ,  G01N33/487 ,  G01N27/447

Abstract: A biochip for molecular detection and sensing is disclosed. The biochip includes a substrate. The biochip includes a plurality of discrete sites formed on the substrate having a density of greater than five hundred wells per square millimeter. Each discrete site includes sidewalls disposed on the substrate to form a well. Each discrete site includes an electrode disposed at the bottom of the well. In some embodiments, the wells are formed such that cross-talk between the wells is reduced. In some embodiments, the electrodes disposed at the bottom of the wells are organized into groups of electrodes, wherein each group of electrodes shares a common counter electrode. In some embodiments, the electrode disposed at the bottom of the well has a dedicated counter electrode. In some embodiments, surfaces of the sidewalls are silanized such that the surfaces facilitate the forming of a membrane in or adjacent to the well.

公开(公告)号：US11505827B2

公开(公告)日：2022-11-22

申请号：US16351456

申请日：2019-03-12

Applicant: Roche Sequencing Solutions, Inc. ,  LIFE TECHNOLOGIES CORPORATION

Inventor： Timothy Geiser ,  Randall Davis ,  Roger Chen

IPC: C12M1/34 ,  C12Q1/6874 ,  C12Q1/6869 ,  B82Y15/00

Abstract: Systems and methods of polynucleotide sequencing are provided. Systems and methods optimize control, speed, movement, and/or translocation of a sample (e.g., a polynucleotide) within, through, or at least partially through a nanopore or a type of protein or mutant protein in order to accumulate sufficient time and current blocking information to identify contiguous nucleotides or plurality of nucleotides in a single-stranded area of a polynucleotide.