公开(公告)号：US20190064141A1

公开(公告)日：2019-02-28

申请号：US16172633

申请日：2018-10-26

Applicant: Genia Technologies, Inc.

Inventor： Roger J.A. Chen ,  Hui Tian ,  Santiago Fernandez-Gomez

IPC: G01N33/487 ,  C12Q1/6869 ,  B82Y15/00

CPC classification number: G01N33/48721 ,  B82Y15/00 ,  C12Q1/6869

Abstract: A method of exporting measurements of a nanopore sensor on a nanopore based sequencing chip is disclosed. An electrical characteristic associated with the nanopore sensor is measured. The electrical characteristic associated with the nanopore sensor is processed. A summary for the electrical characteristic and one or more previous electrical characteristics is determined. The summary for the electrical characteristic and the one or more previous electrical characteristics are exported. Determining the summary includes determining that the electrical characteristic and at least a portion of the one or more previous electrical characteristics correspond to a base call event at the nanopore sensor. The summary represents the electrical characteristic and the at least a portion of the one or more previous electrical characteristics.

公开(公告)号：US20170089857A1

公开(公告)日：2017-03-30

申请号：US14864395

申请日：2015-09-24

Applicant: Genia Technologies, Inc.

Inventor： Bill Maney ,  Hui Tian ,  Santiago Fernandez-Gomez

IPC: G01N27/26 ,  B82Y35/00

CPC classification number: G01N27/26 ,  B82Y35/00 ,  G01N33/48721

Abstract: A nanopore measurement circuit is disclosed. The nanopore measurement circuit includes a nanopore electrode, a first analog memory and a second analog memory. The nanopore measurement circuit also includes a switch network that selectively connects the nanopore electrode to at least one of the first analog and the second analog memory.

公开(公告)号：US09557294B2

公开(公告)日：2017-01-31

申请号：US14577511

申请日：2014-12-19

Applicant: Genia Technologies, Inc.

Inventor： Roger J. A. Chen ,  Hui Tian ,  Bill Maney

IPC: G01N27/447 ,  B01L3/00 ,  C12Q1/68 ,  G01N33/487

CPC classification number: G01N27/44791 ,  B01L3/50273 ,  B01L2400/0421 ,  C12Q1/6869 ,  G01N27/44743 ,  G01N33/48721 ,  C12Q2565/607 ,  C12Q2565/631

Abstract: A method of analyzing a molecule in a nanopore is disclosed. A voltage is applied across a nanopore that is inserted in a membrane by coupling the nanopore to a voltage source. The nanopore is decoupled from the voltage source. After the decoupling, a rate of decay of the voltage across the nanopore is determined. A molecule in the nanopore is distinguished from other possible molecules based on the determined rate of decay of the voltage across the nanopore.

Abstract translation: 公开了分析纳米孔中分子的方法。 通过将纳米孔耦合到电压源，跨越插入膜中的纳米孔施加电压。 纳米孔与电压源分离。 在解耦之后，确定纳米孔上电压的衰减速率。 基于确定的纳米孔上的电压衰减速率，纳米孔中的分子与其它可能的分子区分开来。

公开(公告)号：US20190004028A1

公开(公告)日：2019-01-03

申请号：US16021664

申请日：2018-06-28

Applicant: Genia Technologies, Inc.

Inventor： Jennifer Hovis ,  Hui Tian ,  Roger J.A. Chen

IPC: G01N33/487

CPC classification number: G01N33/48721

Abstract: A nanopore sequencing device is disclosed. The nanopore sequencing device includes a working electrode. It further includes a dielectric layer, wherein a portion of the dielectric layer is disposed horizontally adjacent to the working electrode and a portion of the dielectric layer is disposed above and covering a portion of the working electrode, and wherein the dielectric layer forms a well having an opening above an uncovered portion of the working electrode. A base surface area of the working electrode is greater than a base surface area of the opening above the uncovered portion of the working electrode.

公开(公告)号：US20180328910A1

公开(公告)日：2018-11-15

申请号：US16046931

申请日：2018-07-26

Applicant: Genia Technologies, Inc.

Inventor： Roger J.A. Chen ,  Hui Tian ,  Santiago Fernandez-Gomez

IPC: G01N33/487 ,  C12Q1/6869 ,  B82Y15/00

Abstract: A method of exporting measurements of a nanopore sensor on a nanopore based sequencing chip is disclosed. An electrical characteristic associated with the nanopore sensor is measured. The electrical characteristic associated with the nanopore sensor is processed. A summary for the electrical characteristic and one or more previous electrical characteristics is determined. The summary for the electrical characteristic and the one or more previous electrical characteristics are exported. Determining the summary includes determining that the electrical characteristic and at least a portion of the one or more previous electrical characteristics correspond to a base call event at the nanopore sensor. The summary represents the electrical characteristic and the at least a portion of the one or more previous electrical characteristics.

公开(公告)号：US10036739B2

公开(公告)日：2018-07-31

申请号：US14606632

申请日：2015-01-27

Applicant: Genia Technologies, Inc.

Inventor： Jennifer Hovis ,  Hui Tian ,  Roger J. A. Chen

IPC: G01N27/447 ,  G01N33/487

Abstract: A nanopore sequencing device is disclosed. The nanopore sequencing device includes a working electrode. It further includes a dielectric layer, wherein a portion of the dielectric layer is disposed horizontally adjacent to the working electrode and a portion of the dielectric layer is disposed above and covering a portion of the working electrode, and wherein the dielectric layer forms a well having an opening above an uncovered portion of the working electrode. A base surface area of the working electrode is greater than a base surface area of the opening above the uncovered portion of the working electrode.

公开(公告)号：US09863904B2

公开(公告)日：2018-01-09

申请号：US14971667

申请日：2015-12-16

Applicant: Genia Technologies, Inc.

Inventor： Roger J. A. Chen ,  Hui Tian ,  Bill Maney

IPC: G01N27/447 ,  G01N27/22 ,  C12Q1/68 ,  G01N33/487

CPC classification number: G01N27/228 ,  C12Q1/6869 ,  G01N27/3273 ,  G01N27/44791 ,  G01N33/48721 ,  C12Q2527/125 ,  C12Q2565/607 ,  C12Q2565/629 ,  C12Q2565/631

Abstract: A method of analyzing a molecule is disclosed. A voltage source is selectively connected to or disconnected from a capacitor using a switch controlled by a reset signal. A charge is stored in a capacitor when the voltage source is connected to the capacitor. The capacitor is discharged through a nanopore in a membrane when the voltage source is disconnected from the capacitor. A duty cycle of the reset signal is determined such that the voltage source and the capacitor is connected for at least a one tenth portion of a reset signal period and disconnected for a remaining portion of the reset signal period, such that a voltage across the nanopore is maintained at a higher level during the portion of the reset signal period in which the connection is maintained than during the remaining portion of the reset signal period in which the connection is not maintained.

公开(公告)号：US20190025238A1

公开(公告)日：2019-01-24

申请号：US16141658

申请日：2018-09-25

Applicant: Genia Technologies, Inc.

Inventor： Bill Maney ,  Hui Tian ,  Santiago Fernandez-Gomez

IPC: G01N27/26 ,  G01N33/487 ,  B82Y35/00

Abstract: A nanopore measurement circuit includes a first analog memory configured to store a first electrical value corresponding to a first measurement sample of a nanopore and a second analog memory configured to store a second electrical value corresponding to a second measurement sample of the nanopore. The nanopore measurement circuit also includes a measurement circuitry configured to provide an output indicating a difference between the first electrical value of the first analog memory and the second electrical value of the second analog memory.

公开(公告)号：US20180373837A1

公开(公告)日：2018-12-27

申请号：US16117976

申请日：2018-08-30

Applicant: Genia Technologies, Inc.

Inventor： Santiago Fernandez-Gomez ,  Hui Tian ,  J. William Maney ,  Jayalakshmi Rajaraman

IPC: G06F19/22 ,  G01N33/487

Abstract: A system includes a plurality of nanopore cells. Data corresponding to nanopore states of the plurality of nanopore cells is received. The data is analyzed to determine a compressed output size of the data given at least one compression technique. It is determined whether the compressed output size exceeds a data budget. In the event it is determined that the compressed output size exceeds the data budget, the data is modified. The modified data is outputted.

公开(公告)号：US20160178554A1

公开(公告)日：2016-06-23

申请号：US14971667

申请日：2015-12-16

Applicant: Genia Technologies, Inc.

Inventor： Roger J.A. Chen ,  Hui Tian ,  Bill Maney

IPC: G01N27/22 ,  C12Q1/68 ,  G01N33/487 ,  G01N27/447

CPC classification number: G01N27/228 ,  C12Q1/6869 ,  G01N27/3273 ,  G01N27/44791 ,  G01N33/48721 ,  C12Q2527/125 ,  C12Q2565/607 ,  C12Q2565/629 ,  C12Q2565/631

Abstract: A method of analyzing a molecule is disclosed. A voltage source is selectively connected to or disconnected from a capacitor using a switch controlled by a reset signal. A charge is stored in a capacitor when the voltage source is connected to the capacitor. The capacitor is discharged through a nanopore in a membrane when the voltage source is disconnected from the capacitor. A duty cycle of the reset signal is determined such that the voltage source and the capacitor is connected for at least a one tenth portion of a reset signal period and disconnected for a remaining portion of the reset signal period, such that a voltage across the nanopore is maintained at a higher level during the portion of the reset signal period in which the connection is maintained than during the remaining portion of the reset signal period in which the connection is not maintained.

Abstract translation: 公开了分析分子的方法。 使用由复位信号控制的开关，电压源选择性地连接到电容器或从电容器断开。 当电压源连接到电容器时，电容被存储在电容器中。 当电压源与电容器断开时，电容器通过膜中的纳米孔排出。 确定复位信号的占空比，使得电压源和电容器连接至复位信号周期的至少十分之一，并且对于复位信号周期的剩余部分断开，使得跨越纳米孔的电压 在维持连接的复位信号周期的部分期间保持在比不保持连接的复位信号周期的剩余部分期间更高的电平。