公开(公告)号：US10774347B2

公开(公告)日：2020-09-15

申请号：US15453094

申请日：2017-03-08

Applicant: Braskem S.A.

Inventor： Daniel Johannes Koch ,  Mateus Schreiner Lopes ,  Ane Fernanda Beraldi Zeidler ,  Lucas Pedersen Parizzi

IPC: C12N9/10 ,  C12N1/20 ,  C12P7/28 ,  C12P7/18 ,  C12N9/02 ,  C12N9/12 ,  C12N9/88 ,  C12N9/90 ,  C12N15/52 ,  C12P5/02 ,  C12P7/04 ,  C12P7/26

Abstract: The present application relates to recombinant microorganisms useful in the biosynthesis of monoethylene glycol (MEG) and one or more three-carbon compounds such as acetone, isopropanol or propene. The MEG and one or more three-carbon compounds described herein are useful as starting material for production of other compounds or as end products for industrial and household use. The application further relates to recombinant microorganisms co-expressing a C2 branch pathway and a C3 branch pathway for the production of MEG and one or more three-carbon compounds. Also provided are methods of producing MEG and one or more three-carbon compounds using the recombinant microorganisms, as well as compositions comprising the recombinant microorganisms and/or optionally the products MEG and one or more three-carbon compounds.

公开(公告)号：US10000744B2

公开(公告)日：2018-06-19

申请号：US15023579

申请日：2014-09-23

Applicant: BRASKEM S.A. ,  Avram Michael Slovic

Inventor： Avram Michael Slovic ,  Iuri Estrada Gouvea ,  Daniel Johannes Koch ,  Felipe Galzerani

IPC: C12N1/19 ,  C12N1/21 ,  C12N9/16 ,  C12N9/10 ,  C12P7/04

CPC classification number: C12N9/16 ,  C12N9/13 ,  C12P7/04 ,  C12Y301/02011

Abstract: The disclosure provides engineered enzymes that are capable of mediating the conversion of acetoacetyl-CoA to acetoacetate that do not react with the same order of magnitude with acetyl-CoA as they do with acetoacetyl-CoA (e.g., the engineered enzymes have a specific acetoacetyl-CoA hydrolase activity at least 10× higher than its acetyl-CoA hydrolase activity). Additionally, the disclosure provides modified microorganisms that comprise the engineered enzymes disclosed herein and methods of using same.

公开(公告)号：US11345908B2

公开(公告)日：2022-05-31

申请号：US15327616

申请日：2015-05-29

Applicant: BRASKEM S.A.

Inventor： Avram Michael Slovic ,  Daniel Johannes Koch

IPC: C12N9/90 ,  C12N9/10 ,  C12N9/12 ,  C12P5/00 ,  C12P7/06 ,  C12P19/32

Abstract: The present disclosure generally relates to modified microorganisms comprising an optimized system for oligosaccharide utilization comprising one or more polynucleotides coding for one or more energy independent oligosaccharide transporters for transporting an oligosaccharide into the microorganism, one or more polynucleotides coding for enzymes that catalyze the conversion of the oligosaccharide into at least one phosphorylated saccharide, and one or more polynucleotides coding for enzymes that catalyze the conversion of the phosphorylated saccharide into an isomer of the phosphorylated saccharide that is utilized in one or more enzymatic pathways in the microorganism for the production of an organic molecule such as acetic acid, acrylic acid, 3-hydroxypropionic acid, lactic acid, etc. The present disclosure also generally relates to methods of using the optimized system for oligosaccharide utilization.

公开(公告)号：US11286490B2

公开(公告)日：2022-03-29

申请号：US16317406

申请日：2017-07-12

Applicant: BRASKEM S.A.

Inventor： Mateus Schreiner Lopes ,  Daniel Johannes Koch ,  Iuri Estrada Gouvea ,  Debora Noma Okamoto ,  Veronica Leite Queiroz

IPC: C12P5/02 ,  C12N9/90 ,  C12N9/88 ,  C12N15/52 ,  C12P7/04

Abstract: The present application relates to recombinant microorganisms expressing a dehydratase useful in a one-step, direct fermentative production of one or more primary alkenes from one or more saturated primary or secondary alcohols. Known, well developed high-yielding pathways that use renewable feedstock can be introduced into the recombinant microorganisms to obtain the alcohol precursors. Also provided are methods of producing one or more primary alkenes using the recombinant microorganisms, as well as compositions comprising the recombinant microorganisms and/or optionally the primary alkene products.

公开(公告)号：US10774317B2

公开(公告)日：2020-09-15

申请号：US16011041

申请日：2018-06-18

Applicant: Braskem S.A.

Inventor： Avram Michael Slovic ,  Iuri Estrada Gouvea ,  Daniel Johannes Koch ,  Felipe Galzerani

IPC: C12N9/10 ,  C12N9/16 ,  C12P7/04

Abstract: The disclosure provides engineered enzymes that are capable of mediating the conversion of acetoacetyl-CoA to acetoacetate that do not react with the same order of magnitude with acetyl-CoA as they do with acetoacetyl-CoA (e.g., the engineered enzymes have a specific acetoacetyl-CoA hydrolase activity at least 10× higher than its acetyl-CoA hydrolase activity). Additionally, the disclosure provides modified microorganisms that comprise the engineered enzymes disclosed herein and methods of using same.

公开(公告)号：US20160208228A1

公开(公告)日：2016-07-21

申请号：US15023579

申请日：2014-09-23

Applicant: Avram Michael SLOVIC ,  BRASKEM S.A.

Inventor： Avram Michael Slovic ,  Iuri Estrada Gouvea ,  Daniel Johannes Koch ,  Felipe Galzerani

IPC: C12N9/16

CPC classification number: C12N9/16 ,  C12N9/13 ,  C12P7/04 ,  C12Y301/02011

Abstract: The disclosure provides engineered enzymes that are capable of mediating the conversion of acetoacetyl-CoA to acetoacetate that do not react with the same order of magnitude with acetyl-CoA as they do with acetoacetyl-CoA (e.g., the engineered enzymes have a specific acetoacetyl-CoA hydrolase activity at least 10x higher than its acetyl-CoA hydrolase activity). Additionally, the disclosure provides modified microorganisms that comprise the engineered enzymes disclosed herein and methods of using same.

Abstract translation: 本公开提供了能够介导乙酰乙酰辅酶A向乙酰乙酸酯转化的工程化酶，其与乙酰辅酶A不具有与乙酰辅酶A相同数量级的反应（例如，工程化酶具有特定的乙酰乙酰基 - CoA水解酶活性比其乙酰-CoA水解酶活性高至少10倍）。 另外，本公开提供包含本文公开的工程化酶的修饰的微生物及其使用方法。

公开(公告)号：US20150064759A1

公开(公告)日：2015-03-05

申请号：US14478737

申请日：2014-09-05

Applicant: Braskem S.A.

Inventor： Johana Rincones Perez ,  Juan Diego Rojas Rojas ,  Ane Fernanda Beraldi Zeidler ,  Aline Silva Romao Dumaresq ,  Marilene Elizabete Pavan Rodrigues ,  Iuri Estrada Gouvea ,  Felipe Galzerani ,  Daniel Johannes Koch ,  Lucas Pedersen Parizzi ,  Mateus Schreiner Garcez Lopes ,  Thomas Martin Halder ,  Antonio Luis Ribeiro De Castro Morschbacker ,  Avram Michael Slovic

IPC: C12P7/18 ,  C12P7/04

CPC classification number: C12P7/18 ,  C07K14/395 ,  C12N15/63 ,  C12P5/026 ,  C12P7/04

Abstract: The present disclosure provides a non-naturally occurring microorganism comprising: one or more polynucleotides encoding one or more enzymes in a pathway that produces acetyl-CoA; one or more polynucleotides encoding one or more enzymes in a pathway that catalyze a conversion of cytosolic acetyl-CoA to 2-propanol; one or more polynucleotides encoding one or more enzymes in a pathway that catalyze a conversion of dihydroxyacetone-phosphate to 1-propanol and/or 1,2-propanediol, wherein the microorganism has reduced levels of pyruvate decarboxylase enzymatic activity (e.g., the microorganism comprises a disruption of one or more enzymes that decarboxylate pyruvate and/or a disruption of one or more transcription factors of one or more enzymes that decarboxylate pyruvate), and wherein the microorganism is capable of growing on a C6 sugar as a sole carbon source under anaerobic conditions. Also provided are methods of using the disclosed non-naturally occurring microorganisms in methods for the coproduction of 2-propanol and 1-propanol and/or 1,2-propanediol.

Abstract translation: 本公开提供了非天然存在的微生物，其包含：在产生乙酰辅酶A的途径中编码一种或多种酶的一种或多种多核苷酸; 一种或多种编码催化细胞溶质乙酰辅酶A转化为2-丙醇的途径中的一种或多种酶的多核苷酸; 一种或多种编码一种或多种酶的多核苷酸，其在催化二羟丙基磷酸盐转化为1-丙醇和/或1,2-丙二醇的途径中，其中微生物具有降低的丙酮酸脱羧酶水平的酶活性（例如，微生物包含 破坏一种或多种脱羧酸丙酮酸酶和/或破坏一种或多种丙酮酸脱羧酸酶的一种或多种转录因子的酶），并且其中所述微生物能够生长在C6糖作为唯一碳源的厌氧 条件。 还提供了在共同生产2-丙醇和/或1,2-丙二醇的方法中使用所公开的非天然存在的微生物的方法。