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公开(公告)号：US20210002669A1
公开(公告)日：2021-01-07
申请号：US16956942
申请日：2018-12-21
Applicant: GENENTECH, INC.
Inventor： Chi Kin Domingos Ng , Yongping Guo Crawford , Amy Shen , Meixia Zhou , Bradley R. Snedecor , Shahram Misaghi , Albert Eric Gao
Abstract: The presently disclosed subject matter relates to targeted integration (TI) host cells suitable for the expression of recombinant proteins, as well as methods of producing and using said TI host cells.
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公开(公告)号：US12098365B2
公开(公告)日：2024-09-24
申请号：US17213611
申请日：2021-03-26
Applicant: GENENTECH, INC.
Inventor： Michael Wilson Laird , Shahram Misaghi , Amy Shen , Anthony Tomlinson , Inn Huam Yuk
CPC classification number: C12N15/102 , C12N5/0682 , C12N2510/00
Abstract: The present disclosure relates to methods, cells, and compositions for producing a product of interest, e.g., a recombinant protein. In particular, the present disclosure provides improved mammalian cells expressing the product of interest, where the cells (e.g., Chinese Hamster Ovary (CHO) cells) have reduced or eliminated activity, e.g., expression, of certain host cell proteins, e.g., enzymes including, but not limited to, certain lipases, esterases, and/or hydrolases.
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公开(公告)号：US10704071B2
公开(公告)日：2020-07-07
申请号：US15994950
申请日：2018-05-31
Applicant: Genentech, Inc.
Inventor： Meixia Zhou , Bradley Richard Snedecor , Chi Kin Domingos Ng , Amy Shen
IPC: C12P21/00 , C12N15/113 , C12N1/38 , C12N15/11 , C12N15/85
Abstract: The present invention provides methods and compositions for reducing lactate production and increasing polypeptide production in cultured cells. In one aspect, the invention provides a method comprising culturing cells expressing a) a small interfering RNA (siRNA) specific for a lactate dehydrogenase (LDH) and b) an siRNA specific for a pyruvate dehydrogenase kinase (PDHK). In another aspect, the invention provides cultured cells or vectors comprising an siRNA specific for a LDH and an siRNA specific for a PDHK.
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