公开(公告)号：US12098365B2

公开(公告)日：2024-09-24

申请号：US17213611

申请日：2021-03-26

Applicant: GENENTECH, INC.

Inventor： Michael Wilson Laird ,  Shahram Misaghi ,  Amy Shen ,  Anthony Tomlinson ,  Inn Huam Yuk

IPC: C12N15/10 ,  C12N5/071

CPC classification number: C12N15/102 ,  C12N5/0682 ,  C12N2510/00

Abstract: The present disclosure relates to methods, cells, and compositions for producing a product of interest, e.g., a recombinant protein. In particular, the present disclosure provides improved mammalian cells expressing the product of interest, where the cells (e.g., Chinese Hamster Ovary (CHO) cells) have reduced or eliminated activity, e.g., expression, of certain host cell proteins, e.g., enzymes including, but not limited to, certain lipases, esterases, and/or hydrolases.

公开(公告)号：US20230374497A1

公开(公告)日：2023-11-23

申请号：US18176062

申请日：2023-02-28

Applicant: GENENTECH, INC. ,  HOFFMANN-LA ROCHE INC.

Inventor： Amy SHEN ,  Inn Huam Yuk ,  Peggy Wai No Ko ,  Shahram Misaghi ,  Simon Thomas Auslaender ,  Midori Greenwood-Goodwin ,  Michael Wilson Laird ,  Benedikt Alois Claudius Oswald

IPC: C12N15/10 ,  C12N15/11 ,  C12N9/22 ,  C12N15/90 ,  C12N5/00

CPC classification number: C12N15/1082 ,  C12N15/11 ,  C12N9/22 ,  C12N15/907 ,  C12N5/00 ,  C12N2310/20 ,  C12N2800/80

Abstract: The present disclosure relates to modified mammalian cells having reduced or eliminated expression of certain cellular proteins, CRISPR/Cas9 multiplex knockout strategies for making such cells, and methods of using such cells, e.g., in the context of cell-based therapy or as host cells in the production of a product of interest.

公开(公告)号：US20240010709A1

公开(公告)日：2024-01-11

申请号：US18201904

申请日：2023-05-25

Applicant: Genentech, Inc.

Inventor： Ryan Christopher Hundley ,  Michael Wilson Laird ,  Jerome Joseph Bill ,  Christopher J. Dowd

IPC: C07K16/00 ,  C12P21/00

CPC classification number: C07K16/00 ,  C12P21/005 ,  C07K2317/14

Abstract: Methods for preparing mammalian cells for perfusion cell culture processes that improve the growth and productivity of the cells are provided. Also provided are cell culture methods that involve subjecting mammalian cells to one or more perfusion procedures, which involve generating a solids phase and a liquid phase with a continuous flow centrifuge, and utilizing at least a portion of the solids phase to sustain, maintain and/or initiate a new cell culture. Perfusion bioreactor systems and components thereof are also provided.