公开(公告)号：US12286619B2

公开(公告)日：2025-04-29

申请号：US17036075

申请日：2020-09-29

Applicant: GENENTECH, INC.

Inventor： Shahram Misaghi ,  Masaru Ken Shiratori ,  Bradley Richard Snedecor ,  Michael W. Laird

IPC: C12N15/10 ,  C12N5/071 ,  C12N9/22 ,  C12N15/113

Abstract: The present disclosure relates to methods, cells and compositions for producing a product of interest, e.g., a recombinant protein. In particular, the present disclosure provides improved mammalian cells expressing the product of interests, where the cells (e.g., Chinese Hamster Ovary (CHO) cells) have modulated lactogenic activity. The present disclosure also relates to methods and compositions for modulating pyruvate kinase muscle (PKM) expression (e.g., PKM-1 expression) in a mammalian cell to thereby reduce or eliminate the lactogenic activity of the cell, as well compositions comprising a cell having reduced or eliminated lactogenic activity and methods of using the same.

公开(公告)号：US11987638B1

公开(公告)日：2024-05-21

申请号：US18410949

申请日：2024-01-11

Applicant: Genentech, Inc.

Inventor： Yung-Hsiang Kao ,  Michael W. Laird ,  Melody Trexler Schmidt ,  Rita L. Wong ,  Daniel P. Hewitt

IPC: C07K16/28 ,  A61K39/395 ,  C07K1/14

CPC classification number: C07K16/2887 ,  A61K39/39591 ,  C07K1/14 ,  C07K2317/24

Abstract: The invention concerns methods for preventing the reduction of disulfide bonds during the recombinant production of disulfide-containing polypeptides. In particular, the invention concerns the prevention of disulfide bond reduction during harvesting of disulfide-containing polypeptides, including antibodies, from recombinant host cell cultures.

公开(公告)号：US11976127B1

公开(公告)日：2024-05-07

申请号：US18304286

申请日：2023-04-20

Applicant: Genentech, Inc.

Inventor： Yung-Hsiang Kao ,  Michael W. Laird ,  Melody Trexler Schmidt ,  Rita L. Wong ,  Daniel P. Hewitt

IPC: C07K16/28 ,  A61K39/395 ,  C07K1/14

CPC classification number: C07K16/2887 ,  A61K39/39591 ,  C07K1/14 ,  C07K2317/24

Abstract: The invention concerns methods and means for preventing the reduction of disulfide bonds during the recombinant production of disulfide-containing polypeptides. In particular, the invention concerns the prevention of disulfide bond reduction during harvesting of disulfide-containing polypeptides, including antibodies, from recombinant host cell cultures.

公开(公告)号：US20240132612A1

公开(公告)日：2024-04-25

申请号：US18304286

申请日：2023-04-20

Applicant: Genentech, Inc.

Inventor： Yung-Hsiang Kao ,  Michael W. Laird ,  Melody Trexler Schmidt ,  Rita L. Wong ,  Daniel P. Hewitt

IPC: C07K16/28 ,  A61K39/395 ,  C07K1/14

CPC classification number: C07K16/2887 ,  A61K39/39591 ,  C07K1/14 ,  C07K2317/24

Abstract: The invention concerns methods and means for preventing the reduction of disulfide bonds during the recombinant production of disulfide-containing polypeptides. In particular, the invention concerns the prevention of disulfide bond reduction during harvesting of disulfide-containing polypeptides, including antibodies, from recombinant host cell cultures.

公开(公告)号：US11078294B2

公开(公告)日：2021-08-03

申请号：US17124314

申请日：2020-12-16

Applicant: Genentech, Inc.

Inventor： Yung-Hsiang Kao ,  Michael W. Laird ,  Melody Trexler Schmidt ,  Rita L. Wong ,  Daniel P. Hewitt

IPC: C07K16/28 ,  C07K1/14 ,  A61K39/395

Abstract: The invention concerns methods for preventing the reduction of disulfide bonds during the recombinant production of disulfide-containing polypeptides. In particular, the invention concerns the prevention of disulfide bond reduction during harvesting of disulfide-containing polypeptides, including antibodies, from recombinant host cell cultures.

公开(公告)号：US20190135935A1

公开(公告)日：2019-05-09

申请号：US16240592

申请日：2019-01-04

Applicant: Genentech, Inc.

Inventor： Yung-Hsiang Kao ,  Michael W. Laird ,  Melody Trexler Schmidt ,  Rita L. Wong ,  Daniel P. Hewitt

IPC: C07K16/28 ,  C07K1/14 ,  A61K39/395

Abstract: The invention concerns methods and means for preventing the reduction of disulfide bonds during the recombinant production of disulfide-containing polypeptides. In particular, the invention concerns the prevention of disulfide bond reduction during harvesting of disulfide-containing polypeptides, including antibodies, from recombinant host cell cultures.

公开(公告)号：US10179925B2

公开(公告)日：2019-01-15

申请号：US15816305

申请日：2017-11-17

Applicant: Genentech, Inc.

Inventor： Michael W. Laird ,  Karthik Veeravalli

IPC: C12P21/00 ,  C12P21/02 ,  C12N15/70 ,  C12N9/10 ,  C07K16/18 ,  C07K16/22 ,  C07K16/40 ,  C07K16/28 ,  C07K16/32

Abstract: The present invention relates to methods and compositions for preventing incorporation of norleucine into proteins during recombinant protein production in bacteria. The present invention also provides microorganism host cells and nucleic acid molecules for use with the methods and compositions provided herein.

公开(公告)号：US12145997B2

公开(公告)日：2024-11-19

申请号：US18648209

申请日：2024-04-26

Applicant: Genentech, Inc.

Inventor： Yung-Hsiang Kao ,  Michael W. Laird ,  Melody Trexler Schmidt ,  Rita L. Wong ,  Daniel P. Hewitt

IPC: C07K16/28 ,  A61K39/395 ,  C07K1/14

Abstract: The invention concerns methods for preventing the reduction of disulfide bonds during the recombinant production of disulfide-containing polypeptides. In particular, the invention concerns the prevention of disulfide bond reduction during harvesting of disulfide-containing polypeptides, including antibodies, from recombinant host cell cultures.

公开(公告)号：US11639395B2

公开(公告)日：2023-05-02

申请号：US17087313

申请日：2020-11-02

Applicant: Genentech, Inc.

Inventor： Yung-Hsiang Kao ,  Michael W. Laird ,  Melody Trexler Schmidt ,  Rita L. Wong ,  Daniel P. Hewitt

IPC: C07K16/28 ,  A61K39/395 ,  C07K1/14 ,  A61P5/00 ,  A61P37/02 ,  C07K14/00 ,  C07K16/32 ,  C12N1/38 ,  C07K1/22 ,  C12N5/00

Abstract: The invention concerns methods for preventing the reduction of disulfide bonds during the recombinant production of disulfide-containing polypeptides. In particular, the invention concerns the prevention of disulfide bond reduction during harvesting of disulfide-containing polypeptides, including antibodies, from recombinant host cell cultures.

公开(公告)号：US11015214B2

公开(公告)日：2021-05-25

申请号：US16536149

申请日：2019-08-08

Applicant: Genentech, Inc.

Inventor： Michael W. Laird ,  Karthik Veeravalli

IPC: C12P21/00 ,  C12P21/02 ,  C07K16/22 ,  C07K16/18 ,  C07K16/40 ,  C12N9/10 ,  C12N15/70 ,  C07K16/28 ,  C07K16/32

Abstract: The present invention relates to methods and compositions for preventing incorporation of norleucine into proteins during recombinant protein production in bacteria. The present invention also provides microorganism host cells and nucleic acid molecules for use with the methods and compositions provided herein.