公开(公告)号：US20170073691A1

公开(公告)日：2017-03-16

申请号：US15133150

申请日：2016-04-19

Applicant: Genomatica, Inc.

Inventor： Anthony P. BURGARD ,  Robin E. OSTERHOUT ,  Stephen J. VAN DIEN ,  Cara Ann TRACEWELL ,  Priti PHARKYA ,  Stefan ANDRAE

IPC: C12N15/52 ,  C12N9/10 ,  C12N9/06 ,  C12P7/62 ,  C12N9/04

CPC classification number: C12N15/52 ,  C08F120/06 ,  C08G63/664 ,  C12N9/0006 ,  C12N9/0028 ,  C12N9/1007 ,  C12N15/63 ,  C12P7/24 ,  C12P7/40 ,  C12P7/42 ,  C12P7/62 ,  C12Y101/01244 ,  C12Y105/0102 ,  C12Y201/0109

Abstract: Provided herein is a non-naturally occurring microbial organism having a methanol metabolic pathway that can enhance the availability of reducing equivalents in the presence of methanol. Such reducing equivalents can be used to increase the product yield of organic compounds produced by the microbial organism, such as 3-hydroxyisobutyrate or MAA. Also provided herein are methods for using such an organism to produce 3-hydroxyisobutyrate or MAA.

Abstract translation: 本文提供了具有甲醇代谢途径的非天然存在的微生物生物，其可以在甲醇存在下增强还原当量的可用性。 这种还原当量可用于增加由微生物生物体如3-羟基异丁酸酯或MAA产生的有机化合物的产物产率。 本文还提供了使用这种生物体产生3-羟基异丁酸酯或MAA的方法。