公开(公告)号：US06670120B1

公开(公告)日：2003-12-30

申请号：US09462635

申请日：2000-04-10

申请人： Günter Schmidt ,  Andrew Hugin Thompson

发明人： Günter Schmidt ,  Andrew Hugin Thompson

IPC分类号： C12Q168

CPC分类号： C12Q1/6869 ,  C12Q1/6855 ,  C12Q2525/155 ,  C12Q2521/301

摘要： The present invention involves a method for categorizing nucleic acid which comprises: producing a nucleic acid population by action of an endonuclease on double-stranded nucleic acid, such that each nucleic acid in the nucleic acid population has a double-stranded portion; contacting the nucleic acid population with one or more oligonucleotide sequences; and isolating nucleic acid which correctly hybridizes to an oligonucleotide sequence. In the method of the present invention, each oligonucleotide sequence has a pre-determined recognition sequence. Furthermore, the nucleic acid is categorized by its ability to correctly hybridize to oligonucleotide sequences having the recognition sequence, the recognition sequence being situated such that it recognizes a sequence in the double-stranded portion of the nucleic acid. The oligonucleotide sequence can comprise one or more different recognition sequences.

摘要翻译： 本发明涉及一种用于分类核酸的方法，其包括：通过内切核酸酶在双链核酸上的作用产生核酸群体，使得核酸群体中的每个核酸具有双链部分; 使核酸群体与一个或多个寡核苷酸序列接触; 并分离与寡核苷酸序列正确杂交的核酸。 在本发明的方法中，每个寡核苷酸序列具有预定的识别序列。 此外，核酸通过其与具有识别序列的寡核苷酸序列正确杂交的能力分类，识别序列位于使其识别核酸双链部分中的序列。 寡核苷酸序列可以包含一个或多个不同的识别序列。

公开(公告)号：US08618511B2

公开(公告)日：2013-12-31

申请号：US12212874

申请日：2008-09-18

申请人： Josef K. Röper ,  Werner Finke ,  Martin Frank ,  Günter Schmidt ,  Siegfried Dick ,  Peter Stubenbord

发明人： Josef K. Röper ,  Werner Finke ,  Martin Frank ,  Günter Schmidt ,  Siegfried Dick ,  Peter Stubenbord

IPC分类号： G01N21/00

CPC分类号： G21K5/00 ,  G01N33/48764 ,  G01N35/00029 ,  G01N35/00732 ,  G01N2035/00108 ,  G01N2035/00168

摘要： The test elements are provided that are adapted to detect at least one analyte in a sample. At least some of the test elements are provided with a defect marking which contains information about defectiveness of the test elements. The test elements include at least one radiation-sensitive material. The test elements are exposed to at least one radiation, the radiation being adapted to induce marking in the form of at least one optically detectable change in the radiation-sensitive material.

摘要翻译： 提供了适于检测样品中的至少一种分析物的测试元件。 至少一些测试元件被提供有缺陷标记，该缺陷标记包含关于测试元件的缺陷的信息。 测试元件包括至少一种辐射敏感材料。 测试元件暴露于至少一个辐射，辐射适于以辐射敏感材料中的至少一个可光学检测的变化的形式诱导标记。

公开(公告)号：US06287780B1

公开(公告)日：2001-09-11

申请号：US09581792

申请日：2000-08-11

申请人： Günter Schmidt ,  Andrew Hugin Thompson ,  Robert Alexander Walker Johnstone

发明人： Günter Schmidt ,  Andrew Hugin Thompson ,  Robert Alexander Walker Johnstone

IPC分类号： C12Q168

CPC分类号： C07H23/00 ,  C07H21/00 ,  C12Q1/6816 ,  C12Q1/6837 ,  C12Q1/6872 ,  C12Q1/6874 ,  C40B40/00

摘要： The present invention provides a compound having the following formula: N-L-M wherein N comprises one or more nucleic acid bases, L is either a direct bond between N and M or L comprises a linker moiety, and M comprises a mass marker comprising an aryl ether.

摘要翻译： 本发明提供具有下式的化合物：N-L-M其中N包含一个或多个核酸碱基，L是N和M之间的直接键，或L包含连接体部分，M包括含有芳基醚的质量标记物。

公开(公告)号：US06225077B1

公开(公告)日：2001-05-01

申请号：US09254023

申请日：1999-04-20

申请人： Günter Schmidt ,  Andrew Hugin Thompson

发明人： Günter Schmidt ,  Andrew Hugin Thompson

IPC分类号： C12Q144

CPC分类号： C12N15/1096 ,  C12Q1/6837 ,  C12Q2525/191 ,  C12Q2523/107

摘要： A method for characterizing cDNA, which comprises: (a) cutting a sample comprising a population of one or more cDNAs or isolated fragments thereof, each having a strand complementary to the 3′ poly-A terminus of an mRNA and bearing a tail, with a first sampling endonuclease at a first sampling site of known displacement from a reference site proximal to the tail to generate from each cDNA or isolated fragment thereof a first and second sub-fragment, each comprising a sticky end sequence of predetermined length and unknown sequence, the first sub-fragment bearing the tail; (b) sorting either the first or second sub-fragments into sub-populations according to their sticky end sequence and recording the sticky end sequence of each sub-population as the first sticky end; (c) cutting the sub-fragments of each sub-population with a second sampling endonuclease, which is the same as or different from the first sampling endonuclease, at second sampling site of known displacement from the first sampling site to generate from each sub-fragment a further sub-fragment comprising a second sticky end sequence of predetermined length and unknown sequence; and (d) determining each second sticky end sequence; wherein the aggregate length of the first and second sticky end sequences of each sub-fragment is from 6 to 10; and wherein the sequences and relative positions of the reference site and first and second sticky ends are utilized to characterize said cDNA or cDNAs.

摘要翻译： 一种用于表征cDNA的方法，其包括：（a）切割包含一个或多个cDNA群体或其分离片段的样品，每个cDNA或其分离的片段具有与mRNA的3'poly-A末端互补的并具有尾部的链， 在距离参考站点近尾的已知置换的第一采样位点处的第一取样内切核酸酶从每个cDNA或其分离的片段产生第一和第二子片段，每个子片段包含预定长度和未知序列的粘性末端序列， 第一个带有尾巴的子片段; （b）根据第一或第二子片段的粘性末端序列将第一或第二子片段分成亚群，并记录每个子群体的粘性末端序列作为第一粘性末端; （c）在具有与第一采样内切核酸酶相同或不同于第一取样内切核酸酶的第二取样内切核酸酶切割每个亚群体的亚片段时， 将包含预定长度和未知序列的第二粘性末端序列的另外的子片段片段化; 和（d）确定每个第二粘性末端序列; 其中每个子片段的第一和第二粘性末端序列的聚合长度为6至10; 并且其中使用参考部位和第一和第二粘性末端的序列和相对位置来表征所述cDNA或cDNA。

公开(公告)号：US08448087B2

公开(公告)日：2013-05-21

申请号：US12924522

申请日：2010-09-28

申请人： Günter Schmidt ,  Michael R. Alvers

发明人： Günter Schmidt ,  Michael R. Alvers

IPC分类号： G06F3/00 ,  G06F9/00 ,  G06F17/00

CPC分类号： G06F3/0481

摘要： A computer-implemented method for navigating between sections in a display space is furnished. The sections in the display space are each assigned to a particular location of the display space, and representatives are provided which are each assigned to a particular section in the display space using an unambiguous relation. The method for navigating includes the following steps: selecting at least one representative that is assigned to a particular section in the display space with the aid of the unambiguous relation; and navigating to the particular section in the display space with the aid of the unambiguous relation with the particular section in the display space.

摘要翻译： 提供了一种用于在显示空间中的部分之间导航的计算机实现的方法。 显示空间中的各部分被分配给显示空间的特定位置，并且提供代表，其使用明确的关系分配给显示空间中的特定部分。 用于导航的方法包括以下步骤：借助于明确的关系来选择至少一个分配给显示空间中的特定部分的代表; 并借助于与显示空间中的特定部分的明确关系导航到显示空间中的特定部分。

公开(公告)号：US07523115B2

公开(公告)日：2009-04-21

申请号：US10239446

申请日：2001-03-20

申请人： Günter Schmidt ,  Michael Alvers

发明人： Günter Schmidt ,  Michael Alvers

IPC分类号： G06F17/30

CPC分类号： G06F17/30967 ,  G06F17/30651 ,  Y10S707/99935 ,  Y10S707/99944

摘要： What is disclosed is a computer-implemented method for finding objects which are based on data structures, the method comprising the following steps: announcing a selection of objects; generating and/or invalidating a characterization of one object or several objects of the selection, with characterization being effected by means of an item of characterization information; calculating weights of the objects of the selection and/or of objects located in a vicinity of the characterized objects based on the characterization information, weights already calculated, and/or weights already stored; and modifying the selection and continuing the method from the first step until objects to be found are contained in the selection, until the method is interrupted, or until a given or calculated number of method steps has been carried out, with the possibility of terminating or interrupting the method after any one of the preceding steps.

摘要翻译： 公开的是用于查找基于数据结构的对象的计算机实现的方法，该方法包括以下步骤：通知对象的选择; 产生和/或使一个对象或选择的几个对象的表征无效，其特征是通过表征信息项进行; 基于表征信息，已经计算的权重和/或已经存储的权重来计算选择对象和/或位于特征对象附近的对象的权重; 并且修改选择并从第一步继续该方法，直到找到的对象被包含在选择中，直到该方法被中断，或者直到给定或计算出的方法步骤的数量已被执行，具有可能终止或 在上述任一步骤之后中断该方法。

公开(公告)号：US06500615B1

公开(公告)日：2002-12-31

申请号：US09269555

申请日：1999-05-26

申请人： Günter Schmidt ,  Andrew Hugin Thompson

发明人： Günter Schmidt ,  Andrew Hugin Thompson

IPC分类号： C12Q168

CPC分类号： C12N15/113 ,  C12Q1/6811 ,  C12Q1/6837

摘要： A method for identifying an antisense oligonucleotide capable of binding to a target mRNA, which comprises contacting the target mRNA with each member of an oligonucleotide library separately under hybridization conditions, removing unhybridized material and determining which member or members hybridize; wherein the oligonucleotide library comprises a plurality of distinct nucleotide sequences of a predetermined common length, and wherein each nucleotide sequence comprises a known sequence of 4 to 8 bases and all possible combinations of the known sequence are present in the library.

摘要翻译： 一种用于鉴定能够结合靶mRNA的反义寡核苷酸的方法，其包括在杂交条件下将目标mRNA与寡核苷酸文库的每个成员单独接触，除去未杂交的材料并确定哪些成员或部分杂交; 其中所述寡核苷酸文库包含多个具有预定公共长度的不同核苷酸序列，并且其中每个核苷酸序列包含4至8个碱基的已知序列，并且已知序列的所有可能组合存在于文库中。