-
公开(公告)号：US09382561B2
公开(公告)日：2016-07-05
申请号：US13519526
申请日：2010-12-29
CPC分类号： C12P13/12 , C12N9/1014 , C12N9/88 , C12N15/52 , Y02P20/52
摘要： The present invention relates to a method for the production of methionine using modified strains with attenuated transformation of threonine. This can be achieved by reducing threonine transformation into glycine, and/or by reducing its transformation to α-ketobutyrate. The invention also concerns the modified strains with attenuated transformation of threonine.
摘要翻译： 本发明涉及使用苏氨酸减毒转化的改良菌株生产甲硫氨酸的方法。 这可以通过将苏氨酸转化为甘氨酸和/或通过减少其转化为α-酮丁酸来实现。 本发明还涉及经苏氨酸减毒转化的改良菌株。
-
公开(公告)号：US09121043B2
公开(公告)日：2015-09-01
申请号：US13704578
申请日：2011-06-15
申请人： Wanda Dischert , Rainer Figge , Philippe Soucaille
发明人： Wanda Dischert , Rainer Figge , Philippe Soucaille
CPC分类号： C12P7/42 , C12N15/635 , C12N15/70
摘要： The present invention relates to use of inducible promoters in the production of glycolic acid by fermentation. The present invention concerns a method for the production of glycolic acid in a fermentative process comprising the following steps: culturing a modified microorganism in an appropriate culture medium comprising a source of carbon, modulating in said microorganism the expression of a target gene with an external stimulus, and recovering glycolic acid from the culture medium, wherein in said modified microorganism, the expression of at least one gene involved in glycolic acid production is under the control of a heterologous inducible promoter whose activity is modulated with said external stimulus. The invention also concerned the modified microorganism used in the method of glycolic acid production.
摘要翻译： 本发明涉及通过发酵在诱导型促进剂生产乙醇酸中的用途。 本发明涉及在发酵方法中生产乙醇酸的方法，包括以下步骤：在包含碳源的适当培养基中培养经修饰的微生物，在所述微生物中调节靶基因与外部刺激物的表达 并从培养基中回收乙醇酸，其中在所述修饰的微生物中，参与乙醇酸生产的至少一个基因的表达在异源诱导型启动子的控制下，其活性用所述外部刺激调节。 本发明还涉及用于乙醇酸生产方法中的改性微生物。
-
公开(公告)号：US08748136B2
公开(公告)日：2014-06-10
申请号：US12370422
申请日：2009-02-12
CPC分类号： C12P13/12
摘要： The present invention relates to a method for the production of methionine or its derivatives by culturing a microorganism in an appropriate culture medium comprising a source of carbon and a source of sulphur. The microorganism and/or the culture medium and/or the process parameters were modified in a way that the accumulation of the by-product N-acyl-methionine (NAM) is reduced. The isolation of methionine or its derivatives from the fermentation medium is also claimed.
摘要翻译： 本发明涉及通过在包含碳源和硫源的适当培养基中培养微生物来生产甲硫氨酸或其衍生物的方法。 微生物和/或培养基和/或工艺参数以副产物N-酰基 - 甲硫氨酸（NAM）的积累减少的方式进行修饰。 也要求从发酵培养基中分离甲硫氨酸或其衍生物。
-
公开(公告)号：US20130089903A1
公开(公告)日：2013-04-11
申请号：US13704578
申请日：2011-06-15
申请人： Wanda Dischert , Rainer Figge , Philippe Soucaille
发明人： Wanda Dischert , Rainer Figge , Philippe Soucaille
CPC分类号： C12P7/42 , C12N15/635 , C12N15/70
摘要： The present invention relates to use of inducible promoters in the production of glycolic acid by fermentation. The present invention concerns a method for the production of glycolic acid in a fermentative process comprising the following steps: culturing a modified microorganism in an appropriate culture medium comprising a source of carbon, modulating in said microorganism the expression of a target gene with an external stimulus, and recovering glycolic acid from the culture medium, wherein in said modified microorganism, the expression of at least one gene involved in glycolic acid production is under the control of a heterologous inducible promoter whose activity is modulated with said external stimulus. The invention also concerned the modified microorganism used in the method of glycolic acid production.
摘要翻译： 本发明涉及通过发酵在诱导型促进剂生产乙醇酸中的用途。 本发明涉及在发酵方法中生产乙醇酸的方法，包括以下步骤：在包含碳源的适当培养基中培养经修饰的微生物，在所述微生物中调节靶基因与外部刺激物的表达 并从培养基中回收乙醇酸，其中在所述修饰的微生物中，参与乙醇酸生产的至少一个基因的表达在异源诱导型启动子的控制下，其活性用所述外部刺激调节。 本发明还涉及用于乙醇酸生产方法中的改性微生物。
-
15.发明授权Process for chromosomal integration and DNA sequence replacement in clostridia 有权梭菌染色体整合和DNA序列置换的方法公开(公告)号：US08628967B2
公开(公告)日：2014-01-14
申请号：US13424404
申请日：2012-03-20
IPC分类号： C12N15/74
CPC分类号： C12N15/74
摘要： The present invention is related to a new method for replacing or deleting DNA sequences in Clostridia, with high efficiency, easy to perform and applicable at an industrial level. This method is useful to modify several genetic loci in Clostridia in a routine manner. This method is based on a replicative vector carrying at least two marker genes.
摘要翻译： 本发明涉及一种用于在梭菌中替代或缺失DNA序列的新方法，其在工业上具有高效率，易于实施和适用。 该方法可用于以常规方式修饰梭菌中的几个遗传基因座。 该方法基于携带至少两个标记基因的复制载体。
-
16.发明申请MICRO-ORGANISMS FOR THE PRODUCTION OF ACETOL OBTAINED BY A COMBINATION OF EVOLUTION AND RATIONAL DESIGN 审中-公开通过组合进化和合理设计获得的ACETOL的微生物公开(公告)号：US20110262980A1
公开(公告)日：2011-10-27
申请号：US12532531
申请日：2008-03-21
摘要： The present invention concerns a new method combining evolution and rational design for the preparation of a strain of micro-organism for the production of acetol from a simple carbon source. The said method comprises: growing an initial strain under selection pressure in an appropriate growth medium, said initial bacterial strain comprising an attenuation of the expression of the tpiA gene and an attenuation the expression of at least one gene involved in the conversion of methylglyoxal to lactate, in order to promote evolution in said initial strain, then selecting and isolating the evolved strain having an increased acetol or 1,2-propanediol production rate, then reconstructing a functional tpiA gene in the evolved strain. The present invention also concerns the evolved strain such as obtained, that may be furthermore genetically modified in order to optimize the conversion of a simple carbon source into acetol without by-products and with the best possible yield.
摘要翻译： 本发明涉及一种组合进化和合理设计的新方法，用于制备用于从简单碳源生产丙酮醇的微生物菌株。 所述方法包括：在合适的生长培养基中在选择压力下生长初始菌株，所述初始细菌菌株包括tpiA基因表达的减弱和减少参与将甲基乙二醛转化为乳酸的至少一个基因的表达 为了促进所述初始菌株的进化，然后选择和分离具有增加的丙酮醇或1,2-丙二醇生产速率的进化菌株，然后重建进化菌株中的功能性tpiA基因。 本发明还涉及所获得的进化菌株，其可以进一步经遗传修饰，以便优化简单的碳源转化成不含副产物和最佳产率的丙酮醇。
-
17.发明申请USE OF SUCROSE AS SUBSTRATE FOR FERMENTATIVE PRODUCTION OF 1,2-PROPANEDIOL 审中-公开作为基质用于生产1,2-丙二醇的SUCROSE的使用公开(公告)号：US20110217744A1
公开(公告)日：2011-09-08
申请号：US13127642
申请日：2008-11-07
IPC分类号： C12P7/18
CPC分类号： C12P7/18
摘要： The present invention is relative to a method for producing 1,2-propanediol by fermentation, comprising: cultivating a microorganism producing 1,2-propanediol in an appropriate medium comprising a source of sucrose, and recovering the 1,2-propanediol being produced, wherein the microorganism is able to utilize sucrose as sole carbon source for the production of 1,2-propanediol. In a preferred aspect of the invention, the source of sucrose is obtained from plant biomass, and is in particular sugar cane juice.
摘要翻译： 本发明是相对于通过发酵生产1,2-丙二醇的方法，包括：在合适的培养基中培养产生1,2-丙二醇的微生物，该培养基包含蔗糖源，回收生产的1,2-丙二醇， 其中微生物能够利用蔗糖作为生产1,2-丙二醇的唯一碳源。 在本发明的优选方面，蔗糖源由植物生物质获得，特别是甘蔗汁。
-
18.发明申请METABOLICALLY ENGINEERED MICROORGANISM USEFUL FOR THE PRODUCTION OF ACETOL 审中-公开用于生产ACETOL的有代表性的工程微生物公开(公告)号：US20100279369A1
公开(公告)日：2010-11-04
申请号：US12532460
申请日：2008-03-21
摘要： This invention concerns a microorganism useful for the production of acetol from a simple carbon source, wherein said microorganism is characterized by: an improved activity of the biosynthesis pathway from dihydroxyacetone phosphate to acetol, and an attenuated activity of the glyceraldehyde 3-phosphate dehydrogenase This invention also concerns a method for producing acetol by fermentating a microorganism according to the invention.
摘要翻译： 本发明涉及一种用于从简单碳源生产丙酮醇的微生物，其中所述微生物的特征在于：从磷酸二羟丙酮向丙酮醇的生物合成途径的活性提高，甘油醛-3-磷酸脱氢酶的减毒活性本发明 还涉及通过发酵本发明的微生物来生产丙酮醇的方法。
-
公开(公告)号：US20090325245A1
公开(公告)日：2009-12-31
申请号：US12302726
申请日：2007-06-12
申请人： Philippe Soucaille , Rainer Figge
发明人： Philippe Soucaille , Rainer Figge
IPC分类号： C12P13/00
CPC分类号： C12P13/001
摘要： The present invention provides a bacterium and a method for the biological production of ethanolamine from a fermentable carbon source. In one aspect of the present invention, a process for the conversion of glucose to ethanolamine is achieved by the use of a recombinant bacterium transformed i) to express a serine decarboxylase enzyme to convert serine to ethanolamine ii) to inactivate the ethanolamine consuming pathways and iii) to increase 3-phosphoglycerate availability. In another aspect of the present invention, the process for the production of ethanolamine from glucose using a recombinant E. coli is improved by i) increasing the flux in the serine pathway and ii) decreasing the flux in the serine consuming pathways.
摘要翻译： 本发明提供从可发酵碳源生物制备乙醇胺的细菌和方法。 在本发明的一个方面，通过使用转化的重组细菌i）来表达丝氨酸脱羧酶以将丝氨酸转化为乙醇胺来实现将葡萄糖转化为乙醇胺的方法ii）使乙醇胺消耗途径失活，并且iii ）以提高3-磷酸甘油的可用性。 在本发明的另一方面，使用重组大肠杆菌从葡萄糖生产乙醇胺的方法通过i）增加丝氨酸途径中的通量和ii）降低丝氨酸消耗途径中的通量来改善。
-
20.发明授权公开(公告)号：US09051588B2
公开(公告)日：2015-06-09
申请号：US12532469
申请日：2008-03-21
IPC分类号： C12N1/20 , C12N9/88 , C12N9/02 , C12N15/00 , C12P7/18 , C07H21/04 , C12N15/70 , C12N9/04 , C12P7/26
CPC分类号： C12P7/18 , C12N9/0004 , C12N9/0006 , C12N9/88 , C12N15/70 , C12P7/26
摘要： The present invention concerns a modified microorganism with an increased methylglyoxal reductase activity, and its use for the preparation of 1,2-propanediol and/or acetol. In particular this increased methylglyoxal reductase activity is obtained by increasing the expression of specific genes from microorganisms.This invention is also related to a method for producing 1,2-propanediol and/or acetol by fermentation of a microorganism having an increased methylglyoxal reductase activity.
摘要翻译： 本发明涉及甲基乙二醛还原酶活性增加的修饰微生物及其用于制备1,2-丙二醇和/或丙酮醇的用途。 特别地，通过增加来自微生物的特异性基因的表达来获得增加的甲基乙二醛还原酶活性。 本发明还涉及通过发酵具有增加的甲基乙二醛还原酶活性的微生物来生产1,2-丙二醇和/或丙酮醇的方法。
-
-
-
-
-
-
-
-
-
搜索结果
39 条记录 (耗时 0.028 秒)
