公开(公告)号：US20210088446A1

公开(公告)日：2021-03-25

申请号：US16629202

申请日：2018-04-18

Applicant: HAMAMATSU PHOTONICS K.K.

Inventor： Norikazu SUGIYAMA ,  Masanori MATSUBARA ,  Satoshi YAMAMOTO

IPC: G01N21/64 ,  G01N21/51

Abstract: A sample observation device includes an irradiation unit that irradiates a sample with planar light, a scanning unit that scans the sample in one direction with respect to an irradiation surface of the planar light, an image formation unit that forms images of fluorescent light and scattered light from the sample, an imaging unit that outputs first image data based on a light image of the fluorescent light and second image data based on a light image of the scattered light, an image processing unit that generates a fluorescent light image on the basis of a plurality of pieces of first image data and generates a scattered light image on the basis of a plurality of pieces of second image data, and an analysis unit that specifies an area in which there is the sample in the fluorescent light image on the basis of the scattered light image, and sets an analysis area in the fluorescent light image on the basis of the area in which there is the sample.

公开(公告)号：US20200158633A1

公开(公告)日：2020-05-21

申请号：US16631927

申请日：2018-05-30

Applicant: HAMAMATSU PHOTONICS K.K.

Inventor： Satoshi YAMAMOTO ,  Masanori MATSUBARA ,  Norikazu SUGIYAMA ,  Masanori KOBAYASHI

IPC: G01N21/51 ,  G01N1/20 ,  G02B5/04 ,  G01N21/53

Abstract: A sample observation device includes a flow cell in which a fluid containing samples flows, an irradiation unit configured to irradiate the samples flowing in the flow cell with planar light, an image formation unit having an observation axis inclined with respect to an irradiation surface for the planar light, and configured to form an image of observation light generated in the sample due to the irradiation with the planar light, a two-dimensional imaging element configured to capture a light image including at least a cross section of the fluid among light images according to the observation light formed by the image formation unit, and outputs image data, and an analysis unit configured to analyze a light intensity profile of the sample in a flow direction of the fluid on the basis of the image data.

公开(公告)号：US20200041776A1

公开(公告)日：2020-02-06

申请号：US16340797

申请日：2017-08-15

Applicant: HAMAMATSU PHOTONICS K.K.

Inventor： Satoshi YAMAMOTO ,  Masanori MATSUBARA ,  Norikazu SUGIYAMA

IPC: G02B21/00 ,  G02B21/36

Abstract: A sample observation device (1) includes: an emission optical system (3) for emitting planar light (L2) onto a sample (S); a scanning unit (4) for scanning the sample (S) with respect to an emission face (R) of the planar light (L2); an imaging optical system (5) having an observation axis (P2) inclined with respect to the emission face (R) and for forming an image from observation light (L3) generated in the sample (S) in accordance with the emission of the planar light (L2); an image acquiring unit (6) for acquiring a plurality of partial image data corresponding to a part of an optical image according to the observation light (L3) formed as an image by the imaging optical system (5); and an image generating unit (8) for generating observation image data of the sample S based on the plurality of partial image data generated by the image acquiring unit (6).

公开(公告)号：US20160349170A1

公开(公告)日：2016-12-01

申请号：US15037142

申请日：2014-10-17

Applicant: KYOTO UNIVERSITY ,  HAMAMATSU PHOTONICS K.K.

Inventor： Norio NAKATSUJI ,  Norikazu SUGIYAMA ,  Yoshinori MIZUGUCHI ,  Tadashi FUKAMI ,  Hidenao YAMADA ,  Toyohiko YAMAUCHI ,  Yumi KAKUNO

IPC: G01N15/14 ,  G06K9/00

CPC classification number: G01N15/1434 ,  C12M41/36 ,  G01N15/1475 ,  G01N33/5073 ,  G01N2015/1006 ,  G01N2015/144 ,  G01N2015/1447 ,  G06K9/00134 ,  G06K9/0014 ,  G06K9/00147

Abstract: The present invention relates to a cell assessment method characterized in including an acquisition step of acquiring an optical path length image of a small cell clump, an extraction step of extracting a cell nucleus region within the acquired optical path length image, a comparison step of comparing an optical path length of an inside and an optical path length of an outside of the extracted cell nucleus region, and an assessment step of assessing whether or not a cell is a stem cell based on the comparison results.

Abstract translation: 本发明涉及一种细胞评估方法，其特征在于包括获取小细胞团的光程长度图像的获取步骤，在获取的光程长图像内提取细胞核区域的提取步骤，比较步骤 提取的细胞核区域的内侧的光路长度和光路长度，以及基于比较结果来评价细胞是否为干细胞的评价步骤。