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公开(公告)号：US20220034811A1
公开(公告)日：2022-02-03
申请号：US17488659
申请日：2021-09-29
Applicant: HAMAMATSU PHOTONICS K.K.
Inventor： Norikazu SUGIYAMA , Masanori MATSUBARA , Satoshi YAMAMOTO
Abstract: A sample observation device includes an irradiation unit that irradiates a sample with planar light, a scanning unit that scans the sample in one direction with respect to an irradiation surface of the planar light, an image formation unit that forms images of fluorescent light and scattered light from the sample, an imaging unit that outputs first image data based on a light image of the fluorescent light and second image data based on a light image of the scattered light, an image processing unit that generates a fluorescent light image on the basis of a plurality of pieces of first image data and generates a scattered light image on the basis of a plurality of pieces of second image data, and an analysis unit that specifies an area in which there is the sample in the fluorescent light image on the basis of the scattered light image, and sets an analysis area in the fluorescent light image on the basis of the area in which there is the sample.
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公开(公告)号：US20230222753A1
公开(公告)日：2023-07-13
申请号：US17928992
申请日：2021-06-14
Applicant: HAMAMATSU PHOTONICS K.K.
Inventor： Satoshi YAMAMOTO , Masanori MATSUBARA
IPC: G06V10/143 , G06T7/70 , G06V10/28 , G06V10/60
CPC classification number: G06V10/143 , G06T7/70 , G06V10/28 , G06V10/60 , G06T2207/10152
Abstract: In a sample observation device, an image acquisition unit 6 acquires a plurality of pieces of image data of a sample in a Y-axis direction, and an image generation unit generates luminance image data on luminance of the sample on the basis of the plurality of pieces of image data, binarizes luminance values of each of the plurality of pieces of image data to generate a plurality of pieces of binarized image data, and generates area image data on an existing area of the sample on the basis of the plurality of pieces of binarized image data.
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公开(公告)号：US20210382286A1
公开(公告)日：2021-12-09
申请号：US17411533
申请日：2021-08-25
Applicant: HAMAMATSU PHOTONICS K.K.
Inventor： Satoshi YAMAMOTO , Masanori MATSUBARA , Norikazu SUGIYAMA
Abstract: A sample observation device (1) includes: an emission optical system (3) for emitting planar light (L2) onto a sample (S); a scanning unit (4) for scanning the sample (S) with respect to an emission face (R) of the planar light (L2); an imaging optical system (5) having an observation axis (P2) inclined with respect to the emission face (R) and for forming an image from observation light (L3) generated in the sample (S) in accordance with the emission of the planar light (L2); an image acquiring unit (6) for acquiring a plurality of partial image data corresponding to a part of an optical image according to the observation light (L3) formed as an image by the imaging optical system (5); and an image generating unit (8) for generating observation image data of the sample S based on the plurality of partial image data generated by the image acquiring unit (6).
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公开(公告)号：US20220205913A1
公开(公告)日：2022-06-30
申请号：US17699834
申请日：2022-03-21
Applicant: HAMAMATSU PHOTONICS K.K.
Inventor： Norikazu SUGIYAMA , Masanori MATSUBARA , Satoshi YAMAMOTO
IPC: G01N21/64
Abstract: A sample observation device includes a sample container that holds the sample stained with a first fluorescent substance and a solution including a second fluorescent substance, an irradiation unit that performs irradiation with first excitation light and second excitation light, a scanning unit that scans the sample container in one direction, an image formation unit that forms first fluorescent light from the sample and second fluorescent light from the solution, an imaging unit that outputs first image data based on the first fluorescent light and first image data based on the second fluorescent light, an image processing unit that generates a first fluorescent light image based on a plurality of pieces of first image data and a second fluorescent light image based on a plurality of pieces of second image data, and an analysis unit that specifies an area in which there is the sample on the basis of the second fluorescent light image and sets an analysis area in the first fluorescent light image.
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Patent Agency Ranking
公开(公告)号：US20220283420A1
公开(公告)日：2022-09-08
申请号：US17751818
申请日：2022-05-24
Applicant: HAMAMATSU PHOTONICS K.K.
Inventor： Satoshi YAMAMOTO , Masanori MATSUBARA , Norikazu SUGIYAMA
Abstract: A sample observation device (1) includes: an emission optical system (3) for emitting planar light (L2) onto a sample (S); a scanning unit (4) for scanning the sample (S) with respect to an emission face (R) of the planar light (L2); an imaging optical system (5) having an observation axis (P2) inclined with respect to the emission face (R) and for forming an image from observation light (L3) generated in the sample (S) in accordance with the emission of the planar light (L2); an image acquiring unit (6) for acquiring a plurality of partial image data corresponding to a part of an optical image according to the observation light (L3) formed as an image by the imaging optical system (5); and an image generating unit (8) for generating observation image data of the sample S based on the plurality of partial image data generated by the image acquiring unit (6).
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公开(公告)号：US20210116373A1
公开(公告)日：2021-04-22
申请号：US16629326
申请日：2018-04-10
Applicant: HAMAMATSU PHOTONICS K.K.
Inventor： Norikazu SUGIYAMA , Masanori MATSUBARA , Satoshi YAMAMOTO
Abstract: A sample observation device includes an imaging unit that images observation light generated due to irradiation with the planar light that is transmitted through a membrane filter and outputs fluorescent light image data, a partial image generation unit that specifies a first area corresponding to a first sample holding space and a second area corresponding to a second sample holding space in the fluorescent light image data, and generates first partial image data corresponding to the first area and second partial image data corresponding to the second area, an observation image generation unit that generates first observation image data and second observation image data on the basis of the partial image data, and an analysis unit that analyzes a sample on the basis of the first observation image data and the second observation image data.
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公开(公告)号：US20210088446A1
公开(公告)日：2021-03-25
申请号：US16629202
申请日：2018-04-18
Applicant: HAMAMATSU PHOTONICS K.K.
Inventor： Norikazu SUGIYAMA , Masanori MATSUBARA , Satoshi YAMAMOTO
Abstract: A sample observation device includes an irradiation unit that irradiates a sample with planar light, a scanning unit that scans the sample in one direction with respect to an irradiation surface of the planar light, an image formation unit that forms images of fluorescent light and scattered light from the sample, an imaging unit that outputs first image data based on a light image of the fluorescent light and second image data based on a light image of the scattered light, an image processing unit that generates a fluorescent light image on the basis of a plurality of pieces of first image data and generates a scattered light image on the basis of a plurality of pieces of second image data, and an analysis unit that specifies an area in which there is the sample in the fluorescent light image on the basis of the scattered light image, and sets an analysis area in the fluorescent light image on the basis of the area in which there is the sample.
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公开(公告)号：US20200158633A1
公开(公告)日：2020-05-21
申请号：US16631927
申请日：2018-05-30
Applicant: HAMAMATSU PHOTONICS K.K.
Inventor： Satoshi YAMAMOTO , Masanori MATSUBARA , Norikazu SUGIYAMA , Masanori KOBAYASHI
Abstract: A sample observation device includes a flow cell in which a fluid containing samples flows, an irradiation unit configured to irradiate the samples flowing in the flow cell with planar light, an image formation unit having an observation axis inclined with respect to an irradiation surface for the planar light, and configured to form an image of observation light generated in the sample due to the irradiation with the planar light, a two-dimensional imaging element configured to capture a light image including at least a cross section of the fluid among light images according to the observation light formed by the image formation unit, and outputs image data, and an analysis unit configured to analyze a light intensity profile of the sample in a flow direction of the fluid on the basis of the image data.
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公开(公告)号：US20200041776A1
公开(公告)日：2020-02-06
申请号：US16340797
申请日：2017-08-15
Applicant: HAMAMATSU PHOTONICS K.K.
Inventor： Satoshi YAMAMOTO , Masanori MATSUBARA , Norikazu SUGIYAMA
Abstract: A sample observation device (1) includes: an emission optical system (3) for emitting planar light (L2) onto a sample (S); a scanning unit (4) for scanning the sample (S) with respect to an emission face (R) of the planar light (L2); an imaging optical system (5) having an observation axis (P2) inclined with respect to the emission face (R) and for forming an image from observation light (L3) generated in the sample (S) in accordance with the emission of the planar light (L2); an image acquiring unit (6) for acquiring a plurality of partial image data corresponding to a part of an optical image according to the observation light (L3) formed as an image by the imaging optical system (5); and an image generating unit (8) for generating observation image data of the sample S based on the plurality of partial image data generated by the image acquiring unit (6).
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