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    Fusion proteins having mutated immunoglobulin hinge region
    11.
    发明授权
    Fusion proteins having mutated immunoglobulin hinge region 有权
    具有突变的免疫球蛋白铰链区的融合蛋白

    公开(公告)号：US08067548B2

    公开(公告)日：2011-11-29

    申请号：US12180455

    申请日：2008-07-25

    申请人： Haitao Wang ,  Yong Du ,  Rui Zhang ,  Jing Xu ,  Longbin Liu

    发明人： Haitao Wang ,  Yong Du ,  Rui Zhang ,  Jing Xu ,  Longbin Liu

    IPC分类号： C12P21/08 ,  A61K39/00

    CPC分类号： C12N15/62 ,  A61K47/6811 ,  A61K47/6835 ,  C07K2317/53 ,  C07K2319/30

    摘要： A fusion protein having a non-immunoglobulin polypeptide having a cysteine residue proximal to the C terminal thereof, and an immunoglobulin component with a mutated hinge region is provided. The mutation comprises a point mutated site corresponding in position to the position in a native hinge region of the cysteine residue located nearest the cysteine residue of the non-Ig component. The distance from the cysteine residue of the non-immunoglobulin polypeptide and any remaining cysteine residues of the mutated hinge region is sufficient to prevent the formation of a disulphide bond therebetween.

    摘要翻译： 提供了具有非免疫球蛋白多肽的融合蛋白，其具有接近其C末端的半胱氨酸残基和具有突变的铰链区的免疫球蛋白组分。 该突变包括位于位于最接近非Ig组分的半胱氨酸残基的半胱氨酸残基的天然铰链区的位置的点突变位点。 与非免疫球蛋白多肽的半胱氨酸残基和突变的铰链区的任何剩余半胱氨酸残基的距离足以防止其间形成二硫键。

    RECOMBINANT HUMAN EPO-FC FUSION PROTEINS WITH PROLONGED HALF-LIFE AND ENHANCED ERYTHROPOIETIC ACTIVITY IN VIVO
    12.
    发明申请
    RECOMBINANT HUMAN EPO-FC FUSION PROTEINS WITH PROLONGED HALF-LIFE AND ENHANCED ERYTHROPOIETIC ACTIVITY IN VIVO 有权
    重组人EPO-FC融合蛋白，具有延长的半衰期和增强的生殖活性

    公开(公告)号：US20090297522A1

    公开(公告)日：2009-12-03

    申请号：US12162320

    申请日：2007-01-27

    申请人： Haitao Wang ,  Yong Du ,  Rui Zhang ,  Jing Xu ,  Longbin Liu

    发明人： Haitao Wang ,  Yong Du ,  Rui Zhang ,  Jing Xu ,  Longbin Liu

    IPC分类号： A61K39/395 ,  C07K16/18 ,  C12N15/11 ,  C12N15/00 ,  C12N5/06 ,  C12P21/02

    CPC分类号： C07K14/505 ,  A61K9/0019 ,  A61K38/00 ,  A61K47/6811 ,  C07K2317/53 ,  C07K2319/30

    摘要： A recombinant fusion protein comprising a human erythropoietin peptide portion linked to an immunoglobulin peptide portion is described. The fusion protein has a prolonged half-life in vivo in comparison to naturally occurring or recombinant native human erythropoietin. In one embodiment of the invention the protein has a half-life in vivo at least three fold higher than native human erythropoietin. The fusion protein also exhibits enhanced erythropoietic bioactivity in comparison to native human erythropoietin. In one embodiment, the fusion protein comprises the complete peptide sequence of a human erythropoietin (EPO) molecule and the peptide sequence of an Fc fragment of human immunoglobulin IgG1. The Fc fragment in the fusion protein includes the hinge region, CH2 and CH3 domains of human immunoglobulin IgG1. The EPO molecule may be linked directly to the Fc fragment to avoid extraneous peptide linkers and lessen the risk of an immunogenic response when administered in vivo. In one embodiment the hinge region is a human Fc fragment variant having a non-cysteine residue at amino acid 6. The invention also relates to nucleic acid and amino acid sequences encoding the fusion protein and transfected cell lines and methods for producing the fusion protein. The invention further includes pharmaceutical compositions comprising the fusion protein and methods of using the fusion protein and/or the pharmaceutical compositions, for example to stimulate erythropoiesis in subjects in need of therapy.

    摘要翻译： 描述了包含与免疫球蛋白肽部分连接的人促红细胞生成素肽部分的重组融合蛋白。 与天然存在的或重组的天然人促红细胞生成素相比，融合蛋白在体内具有延长的半衰期。 在本发明的一个实施方案中，蛋白质的体内半衰期比天然人促红细胞生成素高至少三倍。 与天然人促红细胞生成素相比，融合蛋白也表现出增强的红细胞生物活性。 在一个实施方案中，融合蛋白包含人促红细胞生成素（EPO）分子的完整肽序列和人免疫球蛋白IgG1的Fc片段的肽序列。 融合蛋白中的Fc片段包括人免疫球蛋白IgG1的铰链区，CH2和CH3结构域。 EPO分子可以直接连接到Fc片段，以避免外来的肽接头并且当在体内施用时减轻免疫原性应答的风险。 在一个实施方案中，铰链区是在氨基酸6具有非半胱氨酸残基的人Fc片段变体。本发明还涉及编码融合蛋白和转染细胞系的核酸和氨基酸序列以及用于产生融合蛋白的方法。 本发明还包括包含融合蛋白的药物组合物和使用融合蛋白和/或药物组合物的方法，例如刺激需要治疗的受试者的红细胞生成。

    Recombinant human EPO-FC fusion proteins with prolonged half-life and enhanced erythropoietic activity in vivo
    13.
    发明授权
    Recombinant human EPO-FC fusion proteins with prolonged half-life and enhanced erythropoietic activity in vivo 有权
    重组人EPO-FC融合蛋白具有延长的半衰期和体内增强的红细胞生成活性

    公开(公告)号：US08431132B2

    公开(公告)日：2013-04-30

    申请号：US12555743

    申请日：2009-09-08

    申请人： Haitao Wang ,  Du Yong ,  Rui Zhang ,  Jing Xu ,  Longbin Liu

    发明人： Haitao Wang ,  Du Yong ,  Rui Zhang ,  Jing Xu ,  Longbin Liu

    IPC分类号： A61K39/00 ,  C07K1/00 ,  C07K16/00

    CPC分类号： C07K14/505 ,  A61K9/0019 ,  A61K38/00 ,  A61K47/6811 ,  C07K2317/53 ,  C07K2319/30

    摘要： A recombinant fusion protein comprising a human erythropoietin peptide portion linked to an immunoglobulin peptide portion is described. The fusion protein has a prolonged half-life in vivo in comparison to naturally occurring or recombinant native human erythropoietin. In one embodiment of the invention, the protein has a half-life in vivo at least three fold higher than native human erythropoietin. The fusion protein also exhibits enhanced erythropoietic bioactivity in comparison to native human erythropoietin. In one embodiment, the fusion protein comprises the complete peptide sequence of a human erythropoietin (EPO) molecule and the peptide sequence of an Fc fragment of human immunoglobulin IgG1. The Fc fragment in the fusion protein includes the hinge region, CH2 and CH3 domains of human immunoglobulin IgG1. The EPO molecule may be linked directly to the Fc fragment to avoid extraneous peptide linkers and lessen the risk of an immunogenic response when administered in vivo. In one embodiment the hinge region is a human Fc fragment variant having a non-cysteine residue at amino acid 6. The invention also relates to nucleic acid and amino acid sequences encoding the fusion protein and transfected cell lines and methods for producing the fusion protein. The invention further includes pharmaceutical compositions comprising the fusion protein and methods of using the fusion protein and/or the pharmaceutical compositions, for example to stimulate erythropoiesis in subjects in need of therapy.

    摘要翻译： 描述了包含与免疫球蛋白肽部分连接的人促红细胞生成素肽部分的重组融合蛋白。 与天然存在的或重组的天然人促红细胞生成素相比，融合蛋白在体内具有延长的半衰期。 在本发明的一个实施方案中，蛋白质的体内半衰期比天然人促红细胞生成素高至少三倍。 与天然人促红细胞生成素相比，融合蛋白也表现出增强的红细胞生物活性。 在一个实施方案中，融合蛋白包含人促红细胞生成素（EPO）分子的完整肽序列和人免疫球蛋白IgG1的Fc片段的肽序列。 融合蛋白中的Fc片段包括人免疫球蛋白IgG1的铰链区，CH2和CH3结构域。 EPO分子可以直接连接到Fc片段，以避免外来的肽接头并且当在体内施用时减轻免疫原性应答的风险。 在一个实施方案中，铰链区是在氨基酸6具有非半胱氨酸残基的人Fc片段变体。本发明还涉及编码融合蛋白和转染细胞系的核酸和氨基酸序列以及用于产生融合蛋白的方法。 本发明还包括包含融合蛋白的药物组合物和使用融合蛋白和/或药物组合物的方法，例如刺激需要治疗的受试者的红细胞生成。

    Polynucleotides encoding human EPO-FC fusion proteins with prolonged half-life and enhanced erythropoietic activity in vivo
    14.
    发明授权
    Polynucleotides encoding human EPO-FC fusion proteins with prolonged half-life and enhanced erythropoietic activity in vivo 有权
    编码具有延长的半衰期和增强体内红细胞生成活性的人EPO-FC融合蛋白的多核苷酸

    公开(公告)号：US07964375B2

    公开(公告)日：2011-06-21

    申请号：US12555742

    申请日：2009-09-08

    申请人： Haitao Wang ,  Du Yong ,  Rui Zhang ,  Jing Xu ,  Longbin Liu

    发明人： Haitao Wang ,  Du Yong ,  Rui Zhang ,  Jing Xu ,  Longbin Liu

    IPC分类号： A61K39/00 ,  C12P21/06 ,  C12N1/20 ,  C12N15/00 ,  C07K14/00 ,  C07H21/02

    CPC分类号： C07K14/505 ,  A61K9/0019 ,  A61K38/00 ,  A61K47/6811 ,  C07K2317/53 ,  C07K2319/30

    摘要： A recombinant fusion protein comprising a human erythropoietin peptide portion linked to an immunoglobulin peptide portion is described. The fusion protein has a prolonged half-life in vivo in comparison to naturally occurring or recombinant native human erythropoietin. In one embodiment of the invention, the protein has a half-life in vivo at least three fold higher than native human erythropoietin. The fusion protein also exhibits enhanced erythropoietic bioactivity in comparison to native human erythropoietin. In one embodiment, the fusion protein comprises the complete peptide sequence of a human erythropoietin (EPO) molecule and the peptide sequence of an Fc fragment of human immunoglobulin IgG1. The Fc fragment in the fusion protein includes the hinge region, CH2 and CH3 domains of human immunoglobulin IgG1. The EPO molecule may be linked directly to the Fc fragment to avoid extraneous peptide linkers and lessen the risk of an immunogenic response when administered in vivo. In one embodiment the hinge region is a human Fc fragment variant having a non-cysteine residue at amino acid 6. The invention also relates to nucleic acid and amino acid sequences encoding the fusion protein and transfected cell lines and methods for producing the fusion protein. The invention further includes pharmaceutical compositions comprising the fusion protein and methods of using the fusion protein and/or the pharmaceutical compositions, for example to stimulate erythropoiesis in subjects in need of therapy.

    摘要翻译： 描述了包含与免疫球蛋白肽部分连接的人促红细胞生成素肽部分的重组融合蛋白。 与天然存在的或重组的天然人促红细胞生成素相比，融合蛋白在体内具有延长的半衰期。 在本发明的一个实施方案中，蛋白质的体内半衰期比天然人促红细胞生成素高至少三倍。 与天然人促红细胞生成素相比，融合蛋白也表现出增强的红细胞生物活性。 在一个实施方案中，融合蛋白包含人促红细胞生成素（EPO）分子的完整肽序列和人免疫球蛋白IgG1的Fc片段的肽序列。 融合蛋白中的Fc片段包括人免疫球蛋白IgG1的铰链区，CH2和CH3结构域。 EPO分子可以直接连接到Fc片段，以避免外来的肽接头并且当在体内施用时减轻免疫原性应答的风险。 在一个实施方案中，铰链区是在氨基酸6具有非半胱氨酸残基的人Fc片段变体。本发明还涉及编码融合蛋白和转染细胞系的核酸和氨基酸序列以及用于产生融合蛋白的方法。 本发明还包括包含融合蛋白的药物组合物和使用融合蛋白和/或药物组合物的方法，例如刺激需要治疗的受试者的红细胞生成。

    Object pose normalization method and apparatus and object recognition method
    16.
    发明授权
    Object pose normalization method and apparatus and object recognition method 有权
    对象姿态归一化方法及装置与对象识别方法

    公开(公告)号：US08055028B2

    公开(公告)日：2011-11-08

    申请号：US11889427

    申请日：2007-08-13

    申请人： Gyu-tae Park ,  Haitao Wang ,  Young-su Moon

    发明人： Gyu-tae Park ,  Haitao Wang ,  Young-su Moon

    IPC分类号： G06K9/00

    CPC分类号： G06T7/73 ,  G06T2207/30201

    摘要： An object pose normalization method and apparatus and an object recognition method are provided. The object pose normalization method includes: determining a pose of a non-frontal image of an object; performing smoothing transformation on the non-frontal image of the object, thereby generating a smoothed object image; and synthesizing a frontal image of the object by using the pose determination result and the smoothed object image. According to the method and apparatus, a front object image can be synthesized by using a non-frontal object image without causing an image distortion problem due to self-occlusion and non-rigid deformation.

    摘要翻译： 提出了一种物体姿态归一化方法和装置以及物体识别方法。 对象姿态归一化方法包括：确定对象的非正面图像的姿态; 对对象的非正面图像执行平滑变换，从而生成平滑对象图像; 以及通过使用姿势确定结果和平滑的对象图像合成对象的正面图像。 根据该方法和装置，可以通过使用非正面对象图像来合成正面对象图像，而不会由于自闭和非刚性变形而引起图像失真问题。

    SOLAR-POWERED DEVICE
    17.
    发明申请
    SOLAR-POWERED DEVICE 审中-公开
    太阳能设备

    公开(公告)号：US20100001681A1

    公开(公告)日：2010-01-07

    申请号：US12423161

    申请日：2009-04-14

    申请人： Yazhao Zhang ,  Wu Yang ,  Dahong Zhou ,  Haitao Wang

    发明人： Yazhao Zhang ,  Wu Yang ,  Dahong Zhou ,  Haitao Wang

    IPC分类号： H01M10/46 ,  H01L31/048

    CPC分类号： H02J7/355 ,  H01L31/048 ,  H02S40/38 ,  Y02E10/50

    摘要： Solar-powered device having a body for housing at least one energy storage module and at least one control module are disclosed. The body includes an opening formed by at least two recesses disposed about an upper surface of the body. At least one solar panel may be coupled to the upper surface of the body where the solar panel may be received by the recesses.

    摘要翻译： 公开了具有用于容纳至少一个能量存储模块和至少一个控制模块的主体的太阳能装置。 主体包括由围绕主体的上表面设置的至少两个凹部形成的开口。 至少一个太阳能电池板可以耦合到主体的上表面，其中太阳能电池板可以被凹部接收。

    Automatic frequency offset compensation in a TDD wireless OFDM communication system
    18.
    发明申请
    Automatic frequency offset compensation in a TDD wireless OFDM communication system 失效
    TDD无线OFDM通信系统中的自动频偏补偿

    公开(公告)号：US20080137563A1

    公开(公告)日：2008-06-12

    申请号：US11651249

    申请日：2007-01-09

    申请人： Haitao Wang

    发明人： Haitao Wang

    IPC分类号： H04J3/06 ,  H04L7/00 ,  H04L27/06

    CPC分类号： H04L27/266 ,  H04L27/2613 ,  H04L27/2659 ,  H04L27/2675

    摘要： Offsets in the transmit clock in a wireless communication system are corrected by using data distributed over many frequencies. In the time domain the system uses separate copies of the same signal transmitted with a known spacing in terms of transmit clock signals. The variation of timing between the received signals yields an initial estimate that then is used in a closed loop tracking arrangement to yield and compensate for unpredictable rate changes caused by, for example, jitter, Doppler or thermal drift. The frequency offset for one channel can then be used to calculate the offset for other channels.

    摘要翻译： 通过使用分布在许多频率上的数据来校正无线通信系统中发射时钟的偏移。 在时域中，系统使用在传输时钟信号方面以已知间隔传输的相同信号的单独副本。 接收信号之间的定时变化产生初始估计，然后将其用于闭环跟踪装置中以产生并补偿由例如抖动，多普勒或热漂移引起的不可预测的速率变化。 然后可以使用一个通道的频率偏移来计算其他通道的偏移量。