公开(公告)号：US4908313A

公开(公告)日：1990-03-13

申请号：US131281

申请日：1987-12-10

申请人： Yoshiaki Satoh ,  Yasutaka Nakashima ,  Kanehiko Enomoto ,  Atsushi Fujiwara ,  Toshiaki Doi

发明人： Yoshiaki Satoh ,  Yasutaka Nakashima ,  Kanehiko Enomoto ,  Atsushi Fujiwara ,  Toshiaki Doi

IPC分类号： C12N1/20 ,  C12N13/00 ,  C12P1/04 ,  C12P13/02 ,  C12R1/01 ,  C12R1/07 ,  C12R1/13 ,  C12R1/15 ,  C12R1/265 ,  C12R1/365

CPC分类号： C12N13/00 ,  C12P13/02

摘要： In a process for hydrating a nitrile compound by the action of a microorganism having nitrilase activity to convert the nitrile compound into the corresponding amide compound, the performances such as yield and reaction velocity are markedly enhanced by irradiation of the microorganism with light. The process of the present invention is characterized in that (a) the microorganism having nitrilase activity is a positive gram-staining microorganism, (b) the microbial cells are allowed to accept light energy of at least about 1.times.10.sup.-2 .mu.E/g microbial cells second before termination of the hydration reaction, and (c) the hydration reaction is carried out in a vessel composed at least partly of a non-light transmitting material.

摘要翻译： 在通过具有腈水解酶活性的微生物的作用将腈化合物水合化以将腈化合物转化成相应的酰胺化合物的方法中，通过用光照射微生物，显着提高了产率和反应速度等性能。 本发明的方法的特征在于（a）具有腈水解酶活性的微生物是阳性革兰氏染色微生物，（b）允许微生物细胞接受至少约1×10-2微克/微克微生物 在第二次水合反应结束之前的细胞，和（c）水合反应在至少部分由不透光材料组成的容器中进行。

公开(公告)号：US6121025A

公开(公告)日：2000-09-19

申请号：US284966

申请日：1999-05-05

申请人： Eiji Sato ,  Kanehiko Enomoto

发明人： Eiji Sato ,  Kanehiko Enomoto

IPC分类号： C12P17/18 ,  C12P41/00 ,  C12R1/38 ,  C12R1/66 ,  C12R1/72 ,  C12R1/845 ,  C12P17/12 ,  C12P17/10

CPC分类号： C12P17/182 ,  C12P41/004 ,  Y10S435/913 ,  Y10S435/917 ,  Y10S435/918 ,  Y10S435/921 ,  Y10S435/939

摘要： The present invention relates to a process for producing optically active 3-quinuclidinol or derivatives, wherein a racemic 3-quinuclidinol ester represented by the general formula (I): ##STR1## wherein R represents a straight-chain or branched alkyl group, and (H.sup.+) represents that said ester may be in the form of a salt formed with a mineral acid or an organic acid, is reacted with a microorganism belonging to the genus Aspergillus, Rhizopus, Candida or Pseudomonas having the ability to asymmetrically hydrolyze said ester linkage, a culture of said microorganism, a treated material from said microorganism, an enzyme produced by said microorganism, or an enzyme derived from swine or cattle.According to the present invention, there is provided a process for easily producing optically active 3-quinuclidinol derivatives which are important synthetic intermediates for pharmaceutical preparations etc.

摘要翻译： PCT No.PCT / JP97 / 04009 Sec。 371日期1999年5月5日 102（e）日期1999年5月5日PCT 1997年11月4日PCT公布。 WO98 / 20152 PCT出版物 日期：1998年5月14日本发明涉及一种光学活性3-奎宁环醇或其衍生物的制备方法，其中通式（I）表示的外消旋3-奎宁环醇：其中R表示直链或支链烷基， （H +）表示所述酯可以是与无机酸或有机酸形成的盐的形式，与具有不对称水解所述酯键的能力的曲霉属，根霉属，假丝酵母​​属或假单胞菌属的微生物反应 ，所述微生物的培养物，来自所述微生物的处理物，由所述微生物产生的酶，或源自猪或牛的酶。 根据本发明，提供了一种易于制备作为药物制剂等的重要合成中间体的光学活性3-奎宁环醇衍生物的方法

公开(公告)号：US5079153A

公开(公告)日：1992-01-07

申请号：US531701

申请日：1990-06-01

申请人： Kanehiko Enomoto ,  Takao Miyamori ,  Akihiro Sakimae ,  Ryozo Numazawa

发明人： Kanehiko Enomoto ,  Takao Miyamori ,  Akihiro Sakimae ,  Ryozo Numazawa

IPC分类号： C12P17/04

CPC分类号： C12P17/04

摘要： A process for the preparation of an organic ester of ascorbic acid or erythorbic acid represented by the general formula (II) or (III): ##STR1## wherein R.sub.1 is alkyl, aralkyl, or aryl, by reacting ascorbic acid or erythorbic acid with an organic acid or an ester thereof of the general formula (I) :R.sub.1 COOR.sub.2 (I)wherein R.sub.1 is as defined above and R.sub.2 is hydrogen, methyl, ethyl or propyl, in an organic solvent in the presence of an ester hydrolase.