公开(公告)号：US08586947B1

公开(公告)日：2013-11-19

申请号：US13974976

申请日：2013-08-23

Applicant: Illumina, Inc.

Inventor： Wenyi Feng ,  Jason Bryant ,  Dale Buermann

IPC: G01N21/64

CPC classification number: C12Q1/6874 ,  C12Q1/6825 ,  C12Q1/6869 ,  G01N21/05 ,  G01N21/6458 ,  G01N2021/0346 ,  G01N2021/6419 ,  G01N2021/6421 ,  G02B21/0024 ,  G02B2207/113

Abstract: A system and method for imaging biological samples on multiple surfaces of a support structure are disclosed. The support structure may be a flow cell through which a reagent fluid is allowed to flow and interact with the biological samples. Excitation radiation from at least one radiation source may be used to excite the biological samples on multiple surfaces. In this manner, fluorescent emission radiation may be generated from the biological samples and subsequently captured and detected by detection optics and at least one detector. The detected fluorescent emission radiation may then be used to generate image data. This imaging of multiple surfaces may be accomplished either sequentially or simultaneously. In addition, the techniques of the present invention may be used with any type of imaging system. For instance, both epifluorescent and total internal reflection methods may benefit from the techniques of the present invention.

公开(公告)号：US20130023422A1

公开(公告)日：2013-01-24

申请号：US13629949

申请日：2012-09-28

Applicant: Illumina, Inc.

Inventor： Wenyi Feng ,  Jason Bryant ,  Dale Buermann

IPC: C40B20/04

CPC classification number: C12Q1/6874 ,  C12Q1/6825 ,  C12Q1/6869 ,  G01N21/05 ,  G01N21/6458 ,  G01N2021/0346 ,  G01N2021/6419 ,  G01N2021/6421 ,  G02B21/0024 ,  G02B2207/113

Abstract: A system and method for imaging biological samples on multiple surfaces of a support structure are disclosed. The support structure may be a flow cell through which a reagent fluid is allowed to flow and interact with the biological samples. Excitation radiation from at least one radiation source may be used to excite the biological samples on multiple surfaces. In this manner, fluorescent emission radiation may be generated from the biological samples and subsequently captured and detected by detection optics and at least one detector. The detected fluorescent emission radiation may then be used to generate image data. This imaging of multiple surfaces may be accomplished either sequentially or simultaneously. In addition, the techniques of the present invention may be used with any type of imaging system. For instance, both epifluorescent and total internal reflection methods may benefit from the techniques of the present invention.

公开(公告)号：USRE49884E1

公开(公告)日：2024-03-26

申请号：US17214093

申请日：2021-06-08

Applicant: ILLUMINA, INC.

Inventor： Wenyi Feng ,  Jason Bryant ,  Steven Barnard ,  Maria Candelaria Rogert Bacigalupo

IPC: G01N21/64 ,  C12Q1/6825 ,  C12Q1/6869 ,  C12Q1/6874 ,  G01N21/03 ,  G01N21/05 ,  G02B21/00

CPC classification number: G01N21/6458 ,  C12Q1/6825 ,  C12Q1/6869 ,  C12Q1/6874 ,  G01N21/05 ,  G02B21/0024 ,  G01N2021/0346 ,  G01N2021/6419 ,  G01N2021/6421 ,  G02B2207/113

Abstract: A system and method for imaging biological samples on multiple surfaces of a support structure are disclosed. The support structure may be a flow cell through which a reagent fluid is allowed to flow and interact with the biological samples. Excitation radiation from at least one radiation source may be used to excite the biological samples on multiple surfaces. In this manner, fluorescent emission radiation may be generated from the biological samples and subsequently captured and detected by detection optics and at least one detector. The detected fluorescent emission radiation may then be used to generate image data. This imaging of multiple surfaces may be accomplished either sequentially or simultaneously. In addition, the techniques of the present invention may be used with any type of imaging system. For instance, both epifluorescent and total internal reflection methods may benefit from the techniques of the present invention.

公开(公告)号：USRE48561E1

公开(公告)日：2021-05-18

申请号：US16000720

申请日：2018-06-05

Applicant: ILLUMINA, INC.

Inventor： Wenyi Feng ,  Jason Bryant ,  Steven Barnard ,  Maria Candelaria Rogert Bacigalupo

IPC: G01N21/05 ,  G01N21/64 ,  C12Q1/6825 ,  C12Q1/6874 ,  G02B21/00 ,  C12Q1/6869 ,  G01N21/03

Abstract: A system and method for imaging biological samples on multiple surfaces of a support structure are disclosed. The support structure may be a flow cell through which a reagent fluid is allowed to flow and interact with the biological samples. Excitation radiation from at least one radiation source may be used to excite the biological samples on multiple surfaces. In this manner, fluorescent emission radiation may be generated from the biological samples and subsequently captured and detected by detection optics and at least one detector. The detected fluorescent emission radiation may then be used to generate image data. This imaging of multiple surfaces may be accomplished either sequentially or simultaneously. In addition, the techniques of the present invention may be used with any type of imaging system. For instance, both epifluorescent and total internal reflection methods may benefit from the techniques of the present invention.

公开(公告)号：US20130228703A1

公开(公告)日：2013-09-05

申请号：US13858575

申请日：2013-04-08

Applicant: ILLUMINA, INC.

Inventor： Mark Pratt ,  Jason Bryant

IPC: G01N21/64

CPC classification number: G01N21/64 ,  G01J3/4406 ,  G01N21/6428 ,  G01N21/6458 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/6441 ,  G01N2021/6471

Abstract: A detection system including a detection assembly to receive emission light emitted from a sample. The detection assembly has a multi-band dichroic member and at least first and second detection devices. The multi-band dichroic member has a transmission/reflection characteristic with first and second transmissive regions and a reflective region along a wavelength spectrum. The dichroic member transmits portions of the emission light that align with the first and second transmissive regions and reflects a portion of the emission light that aligns with the reflective region. The dichroic member includes a single mirror with an incident surface. The incident surface is configured to transmit the portions of the emission light that align with the first and second transmissive regions and configured to reflect the portion of the emission light that aligns with the reflective region.

Abstract translation: 一种检测系统，包括用于接收从样品发射的发射光的检测组件。 检测组件具有多频带分色构件和至少第一和第二检测装置。 多频带分色构件具有第一和第二透射区域的透射/反射特性以及沿着波长光谱的反射区域。 二向色构件透射与第一和第二透射区域对准的发射光的部分并且反射与反射区域对准的发射光的一部分。 二向色构件包括具有入射表面的单个反射镜。 入射表面被配置为透射与第一和第二透射区域对准的发射光的部分并且被配置为反射与反射区域对准的发射光的部分。

公开(公告)号：US20140213486A1

公开(公告)日：2014-07-31

申请号：US14229455

申请日：2014-03-28

Applicant: Illumina, Inc.

Inventor： Wenyi Feng ,  Jason Bryant ,  Dale Buermann

IPC: C12Q1/68

CPC classification number: C12Q1/6874 ,  C12Q1/6825 ,  C12Q1/6869 ,  G01N21/05 ,  G01N21/6458 ,  G01N2021/0346 ,  G01N2021/6419 ,  G01N2021/6421 ,  G02B21/0024 ,  G02B2207/113

Abstract: A system and method for imaging biological samples on multiple surfaces of a support structure are disclosed. The support structure may be a flow cell through which a reagent fluid is allowed to flow and interact with the biological samples. Excitation radiation from at least one radiation source may be used to excite the biological samples on multiple surfaces. In this manner, fluorescent emission radiation may be generated from the biological samples and subsequently captured and detected by detection optics and at least one detector. The detected fluorescent emission radiation may then be used to generate image data. This imaging of multiple surfaces may be accomplished either sequentially or simultaneously. In addition, the techniques of the present invention may be used with any type of imaging system. For instance, both epifluorescent and total internal reflection methods may benefit from the techniques of the present invention.