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公开(公告)号：USRE49884E1
公开(公告)日：2024-03-26
申请号：US17214093
申请日：2021-06-08
申请人： ILLUMINA, INC.
IPC分类号： G01N21/64 , C12Q1/6825 , C12Q1/6869 , C12Q1/6874 , G01N21/03 , G01N21/05 , G02B21/00
CPC分类号： G01N21/6458 , C12Q1/6825 , C12Q1/6869 , C12Q1/6874 , G01N21/05 , G02B21/0024 , G01N2021/0346 , G01N2021/6419 , G01N2021/6421 , G02B2207/113
摘要： A system and method for imaging biological samples on multiple surfaces of a support structure are disclosed. The support structure may be a flow cell through which a reagent fluid is allowed to flow and interact with the biological samples. Excitation radiation from at least one radiation source may be used to excite the biological samples on multiple surfaces. In this manner, fluorescent emission radiation may be generated from the biological samples and subsequently captured and detected by detection optics and at least one detector. The detected fluorescent emission radiation may then be used to generate image data. This imaging of multiple surfaces may be accomplished either sequentially or simultaneously. In addition, the techniques of the present invention may be used with any type of imaging system. For instance, both epifluorescent and total internal reflection methods may benefit from the techniques of the present invention.
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公开(公告)号：US20230295711A1
公开(公告)日：2023-09-21
申请号：US18008953
申请日：2021-06-24
申请人： Illumina, Inc.
IPC分类号： C12Q1/6869 , G01N27/327
CPC分类号： C12Q1/6869 , G01N27/3276
摘要： Provided herein are compositions and methods for sequencing using at least altering electrical characteristics of polymer bridges. In some examples, the bridges may span the space between first and second electrodes and may include first and second polymer chains that are hybridized to one another. A plurality of nucleotides may be coupled to corresponding labels. A polymerase may be coupled to the bridge and may add nucleotides to a first polynucleotide using at least a sequence of a second polynucleotide. The labels corresponding to those nucleotides respectively may alter hybridization between the first and second polymer chains. Detection circuitry may detect a sequence in which the polymerase adds the nucleotides to the first polynucleotide using at least changes in an electrical signal through the bridge, the changes being responsive to the respective alterations of hybridization using the labels corresponding to those nucleotides.
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公开(公告)号：US11702695B2
公开(公告)日：2023-07-18
申请号：US17662124
申请日：2022-05-05
申请人： Illumina, Inc.
发明人： Yir-Shyuan Wu , Yan-You Lin , M. Shane Bowen , Cyril Delattre , Fabien Abeille , Tarun Khurana , Arnaud Rival , Poorya Sabounchi , Dajun Yuan , Maria Candelaria Rogert Bacigalupo
IPC分类号： B05D3/06 , G03F7/00 , G03F7/20 , C12Q1/6874 , C12Q1/6806
CPC分类号： C12Q1/6874 , B05D3/06 , C12Q1/6806 , G03F7/0002 , G03F7/20
摘要： Embodiments provided herewith are directed to self-assembled methods of preparing a patterned surface for sequencing applications including, for example, a patterned flow cell or a patterned surface for digital fluidic devices. The methods utilize photolithography to create a patterned surface with a plurality of microscale or nanoscale contours, separated by hydrophobic interstitial regions, without the need of oxygen plasma treatment during the photolithography process. In addition, the methods avoid the use of any chemical or mechanical polishing steps after the deposition of a gel material to the contours.
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公开(公告)号：US11384392B2
公开(公告)日：2022-07-12
申请号：US17139834
申请日：2020-12-31
发明人： Jeffrey S. Fisher , Brian D. Mather , Maria Candelaria Rogert Bacigalupo , Justin Fullerton , Ludovic Vincent , Lewis J. Kraft , Sahngki Hong , Boyan Boyanov , M. Shane Bowen , Sang Park , Wayne N. George , Andrew A. Brown
IPC分类号： C12Q1/6869 , C08F220/56 , C08G77/04 , G03F7/075 , G03F7/16 , G03F7/00 , G03F7/004
摘要： An example of a flow cell includes a substrate; a first primer set attached to a first region on the substrate, the first primer set including an un-cleavable first primer and a cleavable second primer; and a second primer set attached to a second region on the substrate, the second primer set including a cleavable first primer and an un-cleavable second primer.
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公开(公告)号：USRE48561E1
公开(公告)日：2021-05-18
申请号：US16000720
申请日：2018-06-05
申请人： ILLUMINA, INC.
IPC分类号： G01N21/05 , G01N21/64 , C12Q1/6825 , C12Q1/6874 , G02B21/00 , C12Q1/6869 , G01N21/03
摘要： A system and method for imaging biological samples on multiple surfaces of a support structure are disclosed. The support structure may be a flow cell through which a reagent fluid is allowed to flow and interact with the biological samples. Excitation radiation from at least one radiation source may be used to excite the biological samples on multiple surfaces. In this manner, fluorescent emission radiation may be generated from the biological samples and subsequently captured and detected by detection optics and at least one detector. The detected fluorescent emission radiation may then be used to generate image data. This imaging of multiple surfaces may be accomplished either sequentially or simultaneously. In addition, the techniques of the present invention may be used with any type of imaging system. For instance, both epifluorescent and total internal reflection methods may benefit from the techniques of the present invention.
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公开(公告)号：US20210024991A1
公开(公告)日：2021-01-28
申请号：US16626452
申请日：2019-06-07
发明人： Jeffrey S. Fisher , Brian D. Mather , Maria Candelaria Rogert Bacigalupo , Justin Fullerton , Ludovic Vincent , Lewis J. Kraft , Sahngki Hong , Boyan Boyanov , M. Shane Bowen , Sang Park , Wayne N. George , Andrew A. Brown , Dajun Yuan
IPC分类号： C12Q1/6869 , G03F7/075 , C08G77/04 , C08F220/56
摘要： An example of a flow cell includes a substrate; a first primer set attached to a first region on the substrate, the first primer set including an un-cleavable first primer and a cleavable second primer; and a second primer set attached to a second region on the substrate, the second primer set including a cleavable first primer and an un-cleavable second primer.
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公开(公告)号：US20170159118A1
公开(公告)日：2017-06-08
申请号：US15436115
申请日：2017-02-17
申请人： Illumina, Inc.
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6869 , C07H21/02 , C07H21/04 , C12N9/00 , C12P19/30 , C12Q1/6874 , C12Q2521/101 , C12Q2521/119 , C12Q2525/101 , C12Q2533/101 , C12Q2533/107 , C12Q2535/122 , C12Q2565/507 , C12Q2565/518 , C12Q2565/537
摘要： A method for sequencing includes steps of (a) providing first and second nucleic acid templates, wherein the two templates have different sequences; (b) extending a first primer bound to the first template using a first polymerase species and a first set of nucleotide analogs; (c) extending a second primer bound to the second template using a second polymerase species and a second set of nucleotide analogs, wherein the first polymerase species is different from the second polymerase species and wherein the first set of nucleotide analog is different from the second set of nucleotide analog, (d) detecting the first and second primer extension products; and (e) repeating steps (b) through (d), thereby determining the different sequences of the first and second templates.
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8.发明申请公开(公告)号：US20160199832A1
公开(公告)日：2016-07-14
申请号：US14912394
申请日：2014-08-29
发明人： Arash Jamshidi , Yan-You Lin , Alex Aravanis , Cyril Delattre , Arnaud Rival , Jennifer Foley , Poorya Sabounchi , Tarun Khurana , Majid Babazadeh , Hamed Amini , Bala Murali Venkatesan , M. Shane Bowen , Steven M. Barnard , Maria Candelaria Rogert Bacigalupo , Dietrich Dehlinger
IPC分类号： B01L3/00
CPC分类号： B01L3/50273 , B01F13/0071 , B01F13/0076 , B01F13/0084 , B01L3/502792 , B01L2200/06 , B01L2300/0636 , B01L2300/0819 , B01L2300/0858 , B01L2300/087 , B01L2300/161 , B01L2300/166 , B01L2400/0427 , B41J2/04 , G01N15/1463 , G01N15/1475 , G01N2015/1006
摘要： Provided herein is a droplet actuator including (a) first and second substrates separated by a droplet-operations gap, the first and second substrates including respective hydrophobic surfaces that face the droplet-operations gap; (b) a plurality of electrodes coupled to at least one of the first substrate and the second substrate, the electrodes arranged along the droplet-operations gap to control movement of a droplet along the hydrophobic surfaces within the droplet-operations gap; and (c) a hydrophilic or variegated-hydrophilic surface exposed to the droplet-operations gap, the hydrophilic or variegated-hydrophilic surface being positioned to contact the droplet when the droplet is at a select position within the droplet-operations gap.
摘要翻译： 本文提供的液滴致动器包括（a）由液滴操作间隙分离的第一和第二基板，第一和第二基板包括面对液滴操作间隙的各个疏水表面; （b）耦合到第一衬底和第二衬底中的至少一个的多个电极，电极沿着液滴操作间隙排列，以控制液滴沿着液滴操作间隙内的疏水表面的运动; 和（c）暴露于液滴操作间隙的亲水或杂色亲水表面，当液滴在液滴操作间隙内的选择位置时，亲水或杂色亲水表面定位成接触液滴。
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公开(公告)号：US11542550B2
公开(公告)日：2023-01-03
申请号：US16626425
申请日：2019-02-13
申请人： Illumina, Inc.
IPC分类号： C12Q1/68 , C07H19/06 , C12Q1/6869 , B01L3/00
摘要： A labeled nucleotide includes a nucleotide, a linking molecule attached to a phosphate group of the nucleotide, and a redox-active charge tag attached to the linking molecule. The redox-active charge tag is to be oxidized or reduced by an electrically conductive channel when maintained in proximity of a sensing zone of the electrically conductive channel.
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公开(公告)号：US20220145379A1
公开(公告)日：2022-05-12
申请号：US17599994
申请日：2020-11-25
发明人： Wayne N. George , Brian D. Mather , Andrew A. Brown , Pietro Gatti Lafranconi , Maria Candelaria Rogert Bacigalupo , Antoine Francais , Xiaohai Liu
IPC分类号： C12Q1/6848 , C12Q1/6869
摘要： An example of a kit includes a flow cell and a cleavage mix. An example flow cell includes a substrate; a catalytic polymeric hydrogel on the substrate, the catalytic polymeric hydrogel including a deblocking catalyst; and an amplification primer attached to the catalytic polymeric hydrogel. The deblocking catalyst accelerates cleavage of a blocking group of a 3′ OH blocked nucleotide introduced to the flow cell and incorporated into a template strand attached to the amplification primer. An example of the cleavage mix includes a component to initiate cleavage of the blocking group.
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