公开(公告)号：US20140319376A1

公开(公告)日：2014-10-30

申请号：US14326128

申请日：2014-07-08

申请人： ILLUMINA, INC.

发明人： Mark Pratt ,  Jason Bryant

IPC分类号： G01N21/64

CPC分类号： G01N21/64 ,  G01J3/4406 ,  G01N21/6428 ,  G01N21/6458 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/6441 ,  G01N2021/6471

摘要： System including an excitation assembly that is configured to excite a sample with first and second wavelengths during first and second excitation events, respectively, of an illumination/detection cycle. The sample provides emission light that includes different first, second, third, and fourth spectral patterns in response to the first and second wavelengths. The system also includes a first detection camera that detects the first spectral pattern during a first measurement phase of the illumination/detection cycle. The first detection camera detects the third spectral pattern during a second measurement phase of the illumination/detection cycle. The system also includes a second detection camera that detects the second spectral pattern during the first measurement phase. The second detection camera detects the fourth spectral pattern during the second measurement phase.

摘要翻译： 该系统包括激励组件，其被配置为分别在照明/检测周期的第一和第二激励事件期间激发具有第一和第二波长的样本。 样品提供响应于第一和第二波长包括不同的第一，第二，第三和第四光谱图案的发射光。 该系统还包括在照明/检测周期的第一测量阶段期间检测第一光谱图案的第一检测照相机。 第一检测照相机在照明/检测周期的第二测量阶段期间检测第三光谱图案。 该系统还包括在第一测量阶段期间检测第二光谱图案的第二检测照相机。 第二检测照相机在第二测量阶段期间检测第四光谱图。

公开(公告)号：US08797535B2

公开(公告)日：2014-08-05

申请号：US13858575

申请日：2013-04-08

申请人： Illumina, Inc.

发明人： Mark Pratt ,  Jason Bryant

IPC分类号： G01N21/00

CPC分类号： G01N21/64 ,  G01J3/4406 ,  G01N21/6428 ,  G01N21/6458 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/6441 ,  G01N2021/6471

摘要： A detection system including a detection assembly to receive emission light emitted from a sample. The detection assembly has a multi-band dichroic member and at least first and second detection devices. The multi-band dichroic member has a transmission/reflection characteristic with first and second transmissive regions and a reflective region along a wavelength spectrum. The dichroic member transmits portions of the emission light that align with the first and second transmissive regions and reflects a portion of the emission light that aligns with the reflective region. The dichroic member includes a single mirror with an incident surface. The incident surface is configured to transmit the portions of the emission light that align with the first and second transmissive regions and configured to reflect the portion of the emission light that aligns with the reflective region.

摘要翻译： 一种检测系统，包括用于接收从样品发射的发射光的检测组件。 检测组件具有多频带分色构件和至少第一和第二检测装置。 多频带分色构件具有第一和第二透射区域的透射/反射特性以及沿着波长光谱的反射区域。 二向色构件透射与第一和第二透射区域对准的发射光的部分并且反射与反射区域对准的发射光的一部分。 二向色构件包括具有入射表面的单个反射镜。 入射表面被配置为透射与第一和第二透射区域对准的发射光的部分并且被配置为反射与反射区域对准的发射光的部分。

公开(公告)号：US08698102B2

公开(公告)日：2014-04-15

申请号：US14056590

申请日：2013-10-17

申请人： Illumina, Inc.

发明人： Wenyi Feng ,  Jason Bryant ,  Dale Buermann

IPC分类号： G01N21/64

CPC分类号： C12Q1/6874 ,  C12Q1/6825 ,  C12Q1/6869 ,  G01N21/05 ,  G01N21/6458 ,  G01N2021/0346 ,  G01N2021/6419 ,  G01N2021/6421 ,  G02B21/0024 ,  G02B2207/113

摘要： A system and method for imaging biological samples on multiple surfaces of a support structure are disclosed. The support structure may be a flow cell through which a reagent fluid is allowed to flow and interact with the biological samples. Excitation radiation from at least one radiation source may be used to excite the biological samples on multiple surfaces. In this manner, fluorescent emission radiation may be generated from the biological samples and subsequently captured and detected by detection optics and at least one detector. The detected fluorescent emission radiation may then be used to generate image data. This imaging of multiple surfaces may be accomplished either sequentially or simultaneously. In addition, the techniques of the present invention may be used with any type of imaging system. For instance, both epifluorescent and total internal reflection methods may benefit from the techniques of the present invention.

摘要翻译： 公开了用于在支撑结构的多个表面上成像生物样品的系统和方法。 支撑结构可以是允许试剂流体通过其流动并与生物样品相互作用的流动池。 来自至少一个辐射源的激发辐射可用于在多个表面上激发生物样品。 以这种方式，可以从生物样品产生荧光发射辐射，随后由检测光学器件和至少一个检测器捕获和检测。 然后可以使用检测到的荧光发射辐射来产生图像数据。 多个表面的这种成像可以顺序地或同时地完成。 此外，本发明的技术可以与任何类型的成像系统一起使用。 例如，荧光和全内反射方法都可以受益于本发明的技术。

公开(公告)号：US09365898B2

公开(公告)日：2016-06-14

申请号：US14721870

申请日：2015-05-26

申请人： Illumina, Inc.

发明人： Wenyi Feng ,  Jason Bryant ,  Dale Buermann

IPC分类号： G01N21/64 ,  C12Q1/68 ,  G01N21/05 ,  G02B21/00 ,  G01N21/03

CPC分类号： C12Q1/6874 ,  C12Q1/6825 ,  C12Q1/6869 ,  G01N21/05 ,  G01N21/6458 ,  G01N2021/0346 ,  G01N2021/6419 ,  G01N2021/6421 ,  G02B21/0024 ,  G02B2207/113

摘要： A system and method for imaging biological samples on multiple surfaces of a support structure are disclosed. The support structure may be a flow cell through which a reagent fluid is allowed to flow and interact with the biological samples. Excitation radiation from at least one radiation source may be used to excite the biological samples on multiple surfaces. In this manner, fluorescent emission radiation may be generated from the biological samples and subsequently captured and detected by detection optics and at least one detector. The detected fluorescent emission radiation may then be used to generate image data. This imaging of multiple surfaces may be accomplished either sequentially or simultaneously. In addition, the techniques of the present invention may be used with any type of imaging system. For instance, both epifluorescent and total internal reflection methods may benefit from the techniques of the present invention.

公开(公告)号：US20140045708A1

公开(公告)日：2014-02-13

申请号：US14056590

申请日：2013-10-17

申请人： Illumina, Inc.

发明人： Wenyi Feng ,  Jason Bryant ,  Dale Buermann

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6874 ,  C12Q1/6825 ,  C12Q1/6869 ,  G01N21/05 ,  G01N21/6458 ,  G01N2021/0346 ,  G01N2021/6419 ,  G01N2021/6421 ,  G02B21/0024 ,  G02B2207/113

摘要： A system and method for imaging biological samples on multiple surfaces of a support structure are disclosed. The support structure may be a flow cell through which a reagent fluid is allowed to flow and interact with the biological samples. Excitation radiation from at least one radiation source may be used to excite the biological samples on multiple surfaces. In this manner, fluorescent emission radiation may be generated from the biological samples and subsequently captured and detected by detection optics and at least one detector. The detected fluorescent emission radiation may then be used to generate image data. This imaging of multiple surfaces may be accomplished either sequentially or simultaneously. In addition, the techniques of the present invention may be used with any type of imaging system. For instance, both epifluorescent and total internal reflection methods may benefit from the techniques of the present invention.

摘要翻译： 公开了用于在支撑结构的多个表面上成像生物样品的系统和方法。 支撑结构可以是允许试剂流体通过其流动并与生物样品相互作用的流动池。 来自至少一个辐射源的激发辐射可用于在多个表面上激发生物样品。 以这种方式，可以从生物样品产生荧光发射辐射，随后由检测光学器件和至少一个检测器捕获和检测。 然后可以使用检测到的荧光发射辐射来产生图像数据。 多个表面的这种成像可以顺序地或同时地完成。 此外，本发明的技术可以与任何类型的成像系统一起使用。 例如，荧光和全内反射方法都可以受益于本发明的技术。

公开(公告)号：US08546772B2

公开(公告)日：2013-10-01

申请号：US13629949

申请日：2012-09-28

申请人： Illumina, Inc.

发明人： Wenyi Feng ,  Jason Bryant ,  Dale Buermann

IPC分类号： G01N21/64

CPC分类号： C12Q1/6874 ,  C12Q1/6825 ,  C12Q1/6869 ,  G01N21/05 ,  G01N21/6458 ,  G01N2021/0346 ,  G01N2021/6419 ,  G01N2021/6421 ,  G02B21/0024 ,  G02B2207/113

摘要： A system and method for imaging biological samples on multiple surfaces of a support structure are disclosed. The support structure may be a flow cell through which a reagent fluid is allowed to flow and interact with the biological samples. Excitation radiation from at least one radiation source may be used to excite the biological samples on multiple surfaces. In this manner, fluorescent emission radiation may be generated from the biological samples and subsequently captured and detected by detection optics and at least one detector. The detected fluorescent emission radiation may then be used to generate image data. This imaging of multiple surfaces may be accomplished either sequentially or simultaneously. In addition, the techniques of the present invention may be used with any type of imaging system. For instance, both epifluorescent and total internal reflection methods may benefit from the techniques of the present invention.

公开(公告)号：USRE48219E1

公开(公告)日：2020-09-22

申请号：US15926322

申请日：2018-03-20

申请人： ILLUMINA, INC.

发明人： Wenyi Feng ,  Jason Bryant ,  Dale Buermann

IPC分类号： C12Q1/6874 ,  C12Q1/6825 ,  G01N21/05 ,  G01N21/64 ,  C12Q1/6869 ,  G01N21/03 ,  G02B21/00

摘要： A system and method for imaging biological samples on multiple surfaces of a support structure are disclosed. The support structure may be a flow cell through which a reagent fluid is allowed to flow and interact with the biological samples. Excitation radiation from at least one radiation source may be used to excite the biological samples on multiple surfaces. In this manner, fluorescent emission radiation may be generated from the biological samples and subsequently captured and detected by detection optics and at least one detector. The detected fluorescent emission radiation may then be used to generate image data. This imaging of multiple surfaces may be accomplished either sequentially or simultaneously. In addition, the techniques of the present invention may be used with any type of imaging system. For instance, both epifluorescent and total internal reflection methods may benefit from the techniques of the present invention.

公开(公告)号：US20150252416A1

公开(公告)日：2015-09-10

申请号：US14721870

申请日：2015-05-26

申请人： Illumina, Inc.

发明人： Wenyi Feng ,  Jason Bryant ,  Dale Buermann

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6874 ,  C12Q1/6825 ,  C12Q1/6869 ,  G01N21/05 ,  G01N21/6458 ,  G01N2021/0346 ,  G01N2021/6419 ,  G01N2021/6421 ,  G02B21/0024 ,  G02B2207/113

摘要： A system and method for imaging biological samples on multiple surfaces of a support structure are disclosed. The support structure may be a flow cell through which a reagent fluid is allowed to flow and interact with the biological samples. Excitation radiation from at least one radiation source may be used to excite the biological samples on multiple surfaces. In this manner, fluorescent emission radiation may be generated from the biological samples and subsequently captured and detected by detection optics and at least one detector. The detected fluorescent emission radiation may then be used to generate image data. This imaging of multiple surfaces may be accomplished either sequentially or simultaneously. In addition, the techniques of the present invention may be used with any type of imaging system. For instance, both epifluorescent and total internal reflection methods may benefit from the techniques of the present invention.

摘要翻译： 公开了用于在支撑结构的多个表面上成像生物样品的系统和方法。 支撑结构可以是允许试剂流体通过其流动并与生物样品相互作用的流动池。 来自至少一个辐射源的激发辐射可用于在多个表面上激发生物样品。 以这种方式，可以从生物样品产生荧光发射辐射，随后由检测光学器件和至少一个检测器捕获和检测。 然后可以使用检测到的荧光发射辐射来产生图像数据。 多个表面的这种成像可以顺序地或同时地完成。 此外，本发明的技术可以与任何类型的成像系统一起使用。 例如，荧光和全内反射方法都可以受益于本发明的技术。

公开(公告)号：US09068220B2

公开(公告)日：2015-06-30

申请号：US14229455

申请日：2014-03-28

申请人： Illumina, Inc.

发明人： Wenyi Feng ,  Jason Bryant ,  Dale Buermann

IPC分类号： C12Q1/68 ,  G01N21/05 ,  G01N21/64 ,  G02B21/00 ,  G01N21/03

CPC分类号： C12Q1/6874 ,  C12Q1/6825 ,  C12Q1/6869 ,  G01N21/05 ,  G01N21/6458 ,  G01N2021/0346 ,  G01N2021/6419 ,  G01N2021/6421 ,  G02B21/0024 ,  G02B2207/113

摘要： A system and method for imaging biological samples on multiple surfaces of a support structure are disclosed. The support structure may be a flow cell through which a reagent fluid is allowed to flow and interact with the biological samples. Excitation radiation from at least one radiation source may be used to excite the biological samples on multiple surfaces. In this manner, fluorescent emission radiation may be generated from the biological samples and subsequently captured and detected by detection optics and at least one detector. The detected fluorescent emission radiation may then be used to generate image data. This imaging of multiple surfaces may be accomplished either sequentially or simultaneously. In addition, the techniques of the present invention may be used with any type of imaging system. For instance, both epifluorescent and total internal reflection methods may benefit from the techniques of the present invention.

摘要翻译： 公开了用于在支撑结构的多个表面上成像生物样品的系统和方法。 支撑结构可以是允许试剂流体通过其流动并与生物样品相互作用的流动池。 来自至少一个辐射源的激发辐射可用于在多个表面上激发生物样品。 以这种方式，可以从生物样品产生荧光发射辐射，随后由检测光学器件和至少一个检测器捕获和检测。 然后可以使用检测到的荧光发射辐射来产生图像数据。 多个表面的这种成像可以顺序地或同时地完成。 此外，本发明的技术可以与任何类型的成像系统一起使用。 例如，荧光和全内反射方法都可以受益于本发明的技术。

公开(公告)号：US08586947B1

公开(公告)日：2013-11-19

申请号：US13974976

申请日：2013-08-23

申请人： Illumina, Inc.

发明人： Wenyi Feng ,  Jason Bryant ,  Dale Buermann

IPC分类号： G01N21/64

CPC分类号： C12Q1/6874 ,  C12Q1/6825 ,  C12Q1/6869 ,  G01N21/05 ,  G01N21/6458 ,  G01N2021/0346 ,  G01N2021/6419 ,  G01N2021/6421 ,  G02B21/0024 ,  G02B2207/113

摘要： A system and method for imaging biological samples on multiple surfaces of a support structure are disclosed. The support structure may be a flow cell through which a reagent fluid is allowed to flow and interact with the biological samples. Excitation radiation from at least one radiation source may be used to excite the biological samples on multiple surfaces. In this manner, fluorescent emission radiation may be generated from the biological samples and subsequently captured and detected by detection optics and at least one detector. The detected fluorescent emission radiation may then be used to generate image data. This imaging of multiple surfaces may be accomplished either sequentially or simultaneously. In addition, the techniques of the present invention may be used with any type of imaging system. For instance, both epifluorescent and total internal reflection methods may benefit from the techniques of the present invention.