公开(公告)号：US20240118188A1

公开(公告)日：2024-04-11

申请号：US18542840

申请日：2023-12-18

Applicant: Illumina, Inc.

Inventor： Helmy A. Eltoukhy ,  Robert C. Kain ,  Wenyi Feng ,  Mark Pratt ,  Bernard Hirschbein ,  Poorya Sabounchi ,  Tarun Khurana

IPC: G01N15/14 ,  B01L3/00 ,  C12Q1/6874

CPC classification number: G01N15/1434 ,  B01L3/502715 ,  C12Q1/6874 ,  G01N15/1404 ,  B01L2300/0654 ,  B01L2300/168

Abstract: A biosensor is provided including a detection device and a flow cell mounted to the detection device. The detection device has a detector surface with a plurality of reaction sites. The detection device also includes a filter layer. A method is providing including obtaining signal data from an array of light detectors; determining a crosstalk function for each of the light detectors of the array of light detectors; and determining characteristics of analytes of interest based on the signal data using the crosstalk functions.

公开(公告)号：US20220276169A1

公开(公告)日：2022-09-01

申请号：US17663476

申请日：2022-05-16

Applicant: Illumina, Inc.

Inventor： Yinghua Sun ,  Stanley S. Hong ,  Frederick Erie ,  Alex Nemiroski ,  M. Shane Bowen ,  Danilo Condello ,  Dietrich Dehlinger ,  Marco A. Krumbuegel ,  Anthony Lam ,  Aaron Liu ,  Bojan Obradovic ,  Mark Pratt

IPC: G01N21/64

Abstract: A system includes: an objective lens; a first light source to feed first illuminating light through the objective lens and into a flowcell (e.g., with a relatively thin film waveguide) to be installed in the system, the first illuminating light to be fed using a first grating on the flowcell; and a first image sensor to capture imaging light using the objective lens, wherein the first grating is positioned outside a field of view of the first image sensor. Dual-surface imaging can be performed. Flowcells with multiple swaths bounded by gratings can be used. An auto-alignment process can be performed.

公开(公告)号：US20210318999A1

公开(公告)日：2021-10-14

申请号：US17304758

申请日：2021-06-25

Applicant: Illumina, Inc.

Inventor： Helmy A. Eltoukhy ,  Robert C. Kain ,  Wenyi Feng ,  Mark Pratt ,  Bernard Hirschbein ,  Poorya Sabounchi ,  Tarun Khurana

IPC: G06F16/21 ,  G01N21/64 ,  G01N33/68

Abstract: A biosensor is provided including a detection device and a flow cell mounted to the detection device. The detection device has a detector surface with a plurality of reaction sites. The detection device also includes a filter layer that is configured to at least one of (a) filter unwanted excitation light signals; (b) direct emission signals from a designated reaction site toward one or more associated light detectors that are configured to detect the emission signals from the designated reaction site; or (c) block or prevent detection of crosstalk emission signals from adjacent reaction sites.

公开(公告)号：US11080248B2

公开(公告)日：2021-08-03

申请号：US15995767

申请日：2018-06-01

Applicant: Illumina, Inc.

Inventor： Helmy A. Eltoukhy ,  Robert C. Kain ,  Wenyi Feng ,  Mark Pratt ,  Bernard Hirschbein ,  Poorya Sabounchi ,  Tarun Khurana

IPC: G06F16/21 ,  G01N21/64 ,  G01N33/68

Abstract: A biosensor is provided including a detection device and a flow cell mounted to the detection device. The detection device has a detector surface with a plurality of reaction sites. The detection device also includes a filter layer that is configured to at least one of (a) filter unwanted excitation light signals; (b) direct emission signals from a designated reaction site toward one or more associated light detectors that are configured to detect the emission signals from the designated reaction site; or (c) block or prevent detection of crosstalk emission signals from adjacent reaction sites.

公开(公告)号：US20150079596A1

公开(公告)日：2015-03-19

申请号：US14552673

申请日：2014-11-25

Applicant: ILLUMINA, INC.

Inventor： Helmy A. Eltoukhy ,  Robert C. Kain ,  Wenyi Feng ,  Mark Pratt ,  Bernard Hirschbein ,  Poorya Sabounchi

IPC: G01N21/64 ,  G01N33/68

CPC classification number: G06F17/303 ,  G01N21/6486 ,  G01N33/6875

Abstract: A biosensor is provided including a detection device and a flow cell mounted to the detection device. The detection device has a detector surface with a plurality of reaction sites. The detection device also includes a filter layer that is configured to at least one of (a) filter unwanted excitation light signals; (b) direct emission signals from a designated reaction site toward one or more associated light detectors that are configured to detect the emission signals from the designated reaction site; or (c) block or prevent detection of crosstalk emission signals from adjacent reaction sites.

Abstract translation: 提供了一种生物传感器，包括检测装置和安装在检测装置上的流通池。 该检测装置具有多个反应位置的检测器表面。 检测装置还包括滤波器层，其被配置为以下中的至少一个：（a）滤除不需要的激发光信号; （b）从被指定的反应部位向被配置成检测来自指定反应部位的发射信号的一个或多个相关联的光检测器的直接发射信号; 或（c）阻止或阻止从相邻反应位点检测串扰发射信号。

公开(公告)号：US20250053547A1

公开(公告)日：2025-02-13

申请号：US18931141

申请日：2024-10-30

Applicant: Illumina, Inc.

Inventor： Helmy A. Eltoukhy ,  Robert C. Kain ,  Wenyi Feng ,  Mark Pratt ,  Bernard Hirschbein ,  Poorya Sabounchi ,  Tarun Khurana

IPC: G06F16/21 ,  B01L3/00 ,  C12Q1/6874 ,  G01N15/1404 ,  G01N15/1434 ,  G01N21/64 ,  G01N33/68

Abstract: A biosensor is provided including a detection device and a flow cell mounted to the detection device. The detection device has a detector surface with a plurality of reaction sites. The detection device also includes a filter layer. A method is providing including obtaining signal data from an array of light detectors; determining a crosstalk function for each of the light detectors of the array of light detectors; and determining characteristics of analytes of interest based on the signal data using the crosstalk functions.

公开(公告)号：US12182084B2

公开(公告)日：2024-12-31

申请号：US18542840

申请日：2023-12-18

Applicant: Illumina, Inc.

Inventor： Helmy A. Eltoukhy ,  Robert C. Kain ,  Wenyi Feng ,  Mark Pratt ,  Bernard Hirschbein ,  Poorya Sabounchi ,  Tarun Khurana

IPC: G06F16/21 ,  B01L3/00 ,  C12Q1/6874 ,  G01N15/1404 ,  G01N15/1434 ,  G01N21/64 ,  G01N33/68

Abstract: A biosensor is provided including a detection device and a flow cell mounted to the detection device. The detection device has a detector surface with a plurality of reaction sites. The detection device also includes a filter layer. A method is providing including obtaining signal data from an array of light detectors; determining a crosstalk function for each of the light detectors of the array of light detectors; and determining characteristics of analytes of interest based on the signal data using the crosstalk functions.

公开(公告)号：US12044624B2

公开(公告)日：2024-07-23

申请号：US17663476

申请日：2022-05-16

Applicant: Illumina, Inc.

Inventor： Yinghua Sun ,  Stanley S. Hong ,  Frederick Erie ,  Alex Nemiroski ,  M. Shane Bowen ,  Danilo Condello ,  Dietrich Dehlinger ,  Marco A. Krumbuegel ,  Anthony Lam ,  Aaron Liu ,  Bojan Obradovic ,  Mark Pratt

IPC: G01N21/64

CPC classification number: G01N21/6456 ,  G01N21/6428 ,  G01N2021/6439 ,  G01N2021/6463 ,  G01N2021/6482

Abstract: A system includes: an objective lens; a first light source to feed first illuminating light through the objective lens and into a flowcell (e.g., with a relatively thin film waveguide) to be installed in the system, the first illuminating light to be fed using a first grating on the flowcell; and a first image sensor to capture imaging light using the objective lens, wherein the first grating is positioned outside a field of view of the first image sensor. Dual-surface imaging can be performed. Flowcells with multiple swaths bounded by gratings can be used. An auto-alignment process can be performed.

公开(公告)号：US11874214B1

公开(公告)日：2024-01-16

申请号：US18179525

申请日：2023-03-07

Applicant: Illumina, Inc.

Inventor： Helmy A. Eltoukhy ,  Robert C. Kain ,  Wenyi Feng ,  Mark Pratt ,  Bernard Hirschbein ,  Poorya Sabounchi ,  Tarun Khurana

IPC: G01N15/14 ,  C12Q1/6874 ,  B01L3/00

CPC classification number: G01N15/1434 ,  B01L3/502715 ,  C12Q1/6874 ,  G01N15/1404 ,  B01L2300/0654 ,  B01L2300/168

Abstract: A biosensor is provided including a detection device and a flow cell mounted to the detection device. The detection device has a detector surface with a plurality of reaction sites. The detection device also includes a filter layer. A method is providing including obtaining signal data from an array of light detectors; determining a crosstalk function for each of the light detectors of the array of light detectors; and determining characteristics of analytes of interest based on the signal data using the crosstalk functions.

公开(公告)号：US09990381B2

公开(公告)日：2018-06-05

申请号：US14552673

申请日：2014-11-25

Applicant: ILLUMINA, INC.

Inventor： Helmy A. Eltoukhy ,  Robert C. Kain ,  Wenyi Feng ,  Mark Pratt ,  Bernard Hirschbein ,  Poorya Sabounchi

IPC: G01N21/64 ,  G06F17/30 ,  G01N33/68

CPC classification number: G06F17/303 ,  G01N21/6486 ,  G01N33/6875

Abstract: Method of analyzing signal data from a biosensor including a detection device having an array of light detectors. The method includes obtaining signal data from the light detectors. The signal data includes light scores that are based on an amount of light detected by the light detectors during a plurality of imaging events. The method also includes analyzing the light scores from a group of light detectors for each of the plurality of the imaging events. The method also includes determining respective crosstalk functions of the light detectors in the group. Each of the crosstalk functions for a corresponding light detector is based on the amount of light detected by other light detectors in the group. The method also includes analyzing the signal data for each of the imaging events using the crosstalk functions to determine characteristics of the analytes-of-interest.