Abstract:
A connector for facilitating the connection of one piece of tubing to another so that fluid can flow from one piece to the other in a sterile manner. Characteristics of the connector include, but are not limited to, a flange at one of the connector, the flange comprising a recess for storage of at least part of a protective shield which Is removable after the flange is connected to the flange of a second connector.
Abstract:
Enclosed reactor systems, each of at least three chambers, fluid flow between the chambers controlled by selectively permeable barriers, flow controlled by an alternating flow diaphragm pump. Also dual diaphragm pump, a diaphragm pump-driven sampling manifold, and a modifier module, all usable with the enclosed reactor systems as well as other systems.
Abstract:
Improved screen filter modules, related compartmentalized filtration modules, and related filtration processes, suitable for filtering fluid to eliminate suspended particulate matter, such as living cells or microcarriers anchoring living cells, or to separate particulate matter based on size. The improvement is the presence of a barrier that channels redirected filtrate to the portion of the filter most susceptible to clogging by the particulate matter and induces flow patterns that act against clogging.
Abstract:
A filtration systems for fluids, particularly biological fluids. The filtration system includes a filter containing compartment connected at one end to a storage vessel and at the other end to a diaphragm pump. The filter comprises a hollow fiber module or a screen filter. The vessel serves as a storage container for a process stream to be filtered. The diaphragm pump provides the means for generating rapid, alternating, low shear tangential flow between the vessel and pump and through the hollow fibers or screen filter. The system allows easy removal of wastes from the fluid and the addition of fresh fluid to replenish the filtered fluid.
Abstract:
A system for electrophoretic analysis includes four tanks, each of which carries an electrolytic solution which accesses a different edge surface of a pair of parallel gel plates. The gel plates include an electrophoretic separation path formed of a selected medium, e.g. an isoelectric focusing gel, extending in a space between the plates and across opposite edges of the plates. A pair of nonpolar barrier webs extend between the plates parallel to and on each side of the path. A different medium, e.g. a running gel, occupies the areas between the gel plates from the opposite sides of the barriers to the remaining edges of the plates. A pair of gaskets are positioned along plate edges which are perpendicular to the path. The gaskets include access apertures for the path and the barriers. A specimen is positioned within a channel leading from an electrolytic solution tank to the path aperture of a gasket and between a dam and the aperture. Electrophoretic separation of the specimen constituents within the separation path is achieved by current flow between a pair of tanks which communicate with the path through the gasket apertures. The barriers are removed preferably by aspiration after specimen separation. An equilibrating medium and gel are infused into the spaces previously occupied by the barriers and electrophoretic separation into the different medium is commenced by current flow between the remaining tanks which access the edges of the gel plates in communication with the different medium.