公开(公告)号：US20160002677A1

公开(公告)日：2016-01-07

申请号：US14494930

申请日：2014-09-24

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Han Min WOO ,  Jung Seok LEE ,  Youngsoon UM ,  Gyeong Taek GONG ,  Byoung Koun MIN ,  Jeong-Myeong HA ,  Yunje KIM ,  Sang Jun SIM

IPC: C12P7/46 ,  C12N15/77

CPC classification number: C12P7/46 ,  C12N15/77

Abstract: The present disclosure relates to a strain capable of producing succinate using starch accumulated in microalgae which grow using carbon dioxide as a direct carbon source without converting it to glucose and a method for producing succinate using the same. The present disclosure provides a strain producing succinate from carbon dioxide, selected from a group consisting of Corynebacterium glutamicum BL-1-pBlAmyS (KCTC 12585BP) and Corynebacterium glutamicum BL-1-pSbAmyA (KCTC 12587BP). The present disclosure also provides a method for producing succinate from carbon dioxide, including fermenting starch by inoculating the strain producing succinate from carbon dioxide in a starch-containing medium.

Abstract translation: 本公开涉及使用在二氧化碳作为直接碳源而不将其转化为葡萄糖而生长的微藻中积累的淀粉生产琥珀酸的菌株，以及使用该淀粉生产琥珀酸盐的方法。 本公开提供了从二氧化碳生产琥珀酸盐的菌株，其选自谷氨酸棒杆菌BL-1-pBlAmyS（KCTC 12585BP）和谷氨酸棒杆菌BL-1-pSbAmyA（KCTC 12587BP）。 本公开还提供了一种从二氧化碳生产琥珀酸的方法，包括通过在含淀粉培养基中从二氧化碳接种产生琥珀酸的菌株来发酵淀粉。

公开(公告)号：US20150275187A1

公开(公告)日：2015-10-01

申请号：US14338770

申请日：2014-07-23

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Han Min WOO ,  Hong-Sil PARK ,  Gyeong Taek Gong ,  Youngsoon UM ,  Yunje KIM ,  Dong Jin SUH

IPC: C12N9/02 ,  C12Q1/68 ,  C12P13/00

CPC classification number: C12Q1/6876 ,  C12N9/0006 ,  C12P13/04 ,  C12Q1/689 ,  C12Q2600/142 ,  C12Y101/03021 ,  C12Y103/01032

Abstract: The furfural-resistant strain containing the furfural-resistant gene according to the present disclosure may be effectively grown in a furfural-containing medium. Accordingly, the problem that microorganism fermentation was difficult because toxic by-products such as furfural are contained in a hydrolysate derived from inedible lignocellulosic biomass may be solved. Further, according to the method for producing a strain of the present disclosure, the resistant gene may be selected from relatively small number of target genes. Thus, time, cost and the like for developing the resistant strain may be saved. Further, this method for identifying genes may be broadly applied to methods for identifying various unknown functional genes in addition to the furfural-resistant gene.

Abstract translation: 含有根据本公开的糠醛抗性基因的糠醛抗性菌株可以有效地在含糠醛的培养基中生长。 因此，可以解决微生物发酵困难的问题，因为衍生自不可食用的木质纤维素生物质的水解产物中含有糠醛等有毒副产物。 此外，根据本公开的菌株的制造方法，抗性基因可以选自相对少量的靶基因。 因此，可以节省开发耐药菌株的时间，成本等。 此外，用于鉴定基因的方法可广泛地应用于除了糠醛抗性基因之外鉴定各种未知功能基因的方法。