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11.发明申请公开(公告)号：US20090099081A1
公开(公告)日：2009-04-16
申请号：US12209354
申请日：2008-09-12
IPC分类号： A61K38/16 , C12N15/11 , C12N15/00 , C12N1/21 , C12N5/04 , C12P21/04 , A01H5/00 , C07K14/00 , C07K16/18
CPC分类号： C07K14/325 , C12N15/8286 , Y02A40/162
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided, particularly synthetically-derived coding sequences. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:9, 11, 13, 15, or 18, or the nucleotide sequence set forth in SEQ ID NO:1, 2, 4, 6, 7, 8, 10, 12, 14, 16, or 17, as well as variants and fragments thereof.
摘要翻译： 提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。 提供包含δ-内毒素多肽的编码序列的组合物，特别是合成来源的编码序列。 编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了分离的δ-内毒素核酸分子。 另外，包括对应于多核苷酸的氨基酸序列，以及与这些氨基酸序列特异性结合的抗体。 特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：9,11,13,15或18所示氨基酸序列的核苷酸序列或SEQ ID NO：1所示的核苷酸序列， 2，4，6，7，8，10，12，14，16或17，以及其变体和片段。
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12.发明授权公开(公告)号：US08541366B2
公开(公告)日：2013-09-24
申请号：US12209354
申请日：2008-09-12
IPC分类号： A01N37/18 , C07K14/325
CPC分类号： C07K14/325 , C12N15/8286 , Y02A40/162
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided, particularly synthetically-derived coding sequences. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:9, 11, 13, 15, or 18, or the nucleotide sequence set forth in SEQ ID NO:1, 2, 4, 6, 7, 8, 10, 12, 14, 16, or 17, as well as variants and fragments thereof.
摘要翻译： 提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。 提供包含δ-内毒素多肽的编码序列的组合物，特别是合成来源的编码序列。 编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了分离的δ-内毒素核酸分子。 另外，包括对应于多核苷酸的氨基酸序列，以及与这些氨基酸序列特异性结合的抗体。 特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：9,11,13,15或18所示氨基酸序列的核苷酸序列或SEQ ID NO：1所示的核苷酸序列， 2，4，6，7，8，10，12，14，16或17，以及其变体和片段。
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13.发明授权AXMI-031, AXMI-039, AXMI-040 and AXMI-049, a family of novel delta endotoxin genes and methods for their use 有权AXMI-031，AXMI-039，AXMI-040和AXMI-049，一种新型的δ内毒素基因及其使用方法公开(公告)号：US07923602B2
公开(公告)日：2011-04-12
申请号：US11762886
申请日：2007-06-14
申请人： Nadine Carozzi , Nicholas Duck , Theodore Kahn , Nalini Desai
发明人： Nadine Carozzi , Nicholas Duck , Theodore Kahn , Nalini Desai
CPC分类号： C07K14/325 , C12N15/8285 , C12N15/8286 , Y02A40/162 , Y02A40/164
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, 4, 6, 8, 10, 12, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, or 38, or the nucleotide sequence set forth in SEQ ID NO: 1, 3, 5, 7, 9, 11, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, or 37, as well as variants and fragments thereof.
摘要翻译： 提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。 提供了包含δ-内毒素多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了分离的δ-内毒素核酸分子。 另外，包括对应于多核苷酸的氨基酸序列，以及与这些氨基酸序列特异性结合的抗体。 具体地说，本发明提供了分离的核酸分子，其包含编码SEQ ID NO： 27,29,31,33,35或38所示的核苷酸序列，或SEQ ID NO：1,3,5,7,9,11,14,16,18,20,22,24,26， 28，30，32，34或37，以及其变体和片段。
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14.发明申请AXMI-031, AXMI-039, AXMI-040 AND AXMI-049, A FAMILY OF NOVEL DELTA-ENDOTOXIN GENES AND METHODS FOR THEIR USE 有权AXMI-031，AXMI-039，AXMI-040和AXMI-049，新型内啡肽基因家族及其使用方法公开(公告)号：US20090181116A1
公开(公告)日：2009-07-16
申请号：US12364335
申请日：2009-02-02
申请人： Nadine Carozzi , Nicholas Duck , Theodore Kahn , Nalini Desai , Shruti Agarwal , Daniel John Tomso , Rong Guo , Julia Daum
发明人： Nadine Carozzi , Nicholas Duck , Theodore Kahn , Nalini Desai , Shruti Agarwal , Daniel John Tomso , Rong Guo , Julia Daum
CPC分类号： C07K14/325 , C12N15/8285 , C12N15/8286 , Y02A40/162 , Y02A40/164
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, 4, 6, 8, 10, 12, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, or 38, or the nucleotide sequence set forth in SEQ ID NO:1, 3, 5, 7, 9, 11, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, or 37, as well as variants and fragments thereof.
摘要翻译： 提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。 提供了包含δ-内毒素多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了分离的δ-内毒素核酸分子。 另外，包括对应于多核苷酸的氨基酸序列，以及与这些氨基酸序列特异性结合的抗体。 具体地说，本发明提供了分离的核酸分子，其包含编码SEQ ID NO： 27,29,31,33,35或38所示的核苷酸序列，或SEQ ID NO：1,3,5,7,9,11,14,16,18,20,22,24,26， 28，30，32，34或37，以及其变体和片段。
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15.发明申请AXMI-066 AND AXMI-076: DELTA-ENDOTOXIN PROTEINS AND METHODS FOR THEIR USE 审中-公开AXMI-066和AXMI-076：DELTA-内毒素蛋白及其使用方法公开(公告)号：US20090144852A1
公开(公告)日：2009-06-04
申请号：US12252453
申请日：2008-10-16
申请人： Daniel J. Tomso , Nalini Desai , Shruti Agarwal , Kimberly S. Sampson , Sandra Volrath , Volker Heinrichs
发明人： Daniel J. Tomso , Nalini Desai , Shruti Agarwal , Kimberly S. Sampson , Sandra Volrath , Volker Heinrichs
IPC分类号： C12N15/82 , A01H5/00 , C12N15/11 , C12N15/00 , C12N1/21 , C12N5/04 , C07K14/00 , A61K38/16 , C12P21/02
CPC分类号： C07K14/325 , C12N15/8285 , C12N15/8286 , Y02A40/162 , Y02A40/164
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for pesticidal polypeptides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated pesticidal nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:5, 2, or 10, the nucleotide sequence set forth in SEQ ID NO:4, 1, 3, 4, 6, 9, or 11, or the nucleotide sequence deposited in a bacterial host as Accession No. B-50045, as well as variants and fragments thereof.
摘要翻译： 提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。 提供了包含杀虫多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了分离的杀虫核酸分子。 另外，包括对应于多核苷酸的氨基酸序列。 特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：5,2或10所示氨基酸序列的核苷酸序列，SEQ ID NO：4,1,3,4 ，6,9或11个核苷酸序列，或以细菌宿主保藏号B-50045以及其变体和片段沉积的核苷酸序列。
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公开(公告)号：US20090126044A1
公开(公告)日：2009-05-14
申请号：US12249016
申请日：2008-10-10
申请人： Nadine Carozzi , Nalini Desai , Daniel J. Tomso
发明人： Nadine Carozzi , Nalini Desai , Daniel J. Tomso
CPC分类号： C12N15/8286 , C07K14/325 , Y02A40/162
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions containing a synthetic nucleotide sequence encoding a Cry1Ac protein are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated pesticidal nucleic acid molecules are provided, wherein the nucleotide sequences are set forth in SEQ ID NO:1, 2, 3, 4, 5 or 6, as well as variants and fragments thereof.
摘要翻译： 提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。 提供了含有编码Cry1Ac蛋白质的合成核苷酸序列的组合物。 编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了分离的杀虫核酸分子，其中核苷酸序列如SEQ ID NO：1,2,3,4,5或6所示，以及其变体和片段。
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17.发明申请AXMI-031, AXMI-039, AXMI-040 AND AXMI-049, A FAMILY OF NOVEL DELTA ENDOTOXIN GENES AND METHODS FOR THEIR USE 有权AXMI-031，AXMI-039，AXMI-040和AXMI-049，新型内啡肽基因的家族及其使用方法公开(公告)号：US20070294787A1
公开(公告)日：2007-12-20
申请号：US11762886
申请日：2007-06-14
申请人： Nadine Carozzi , Nicholas Duck , Theodore Kahn , Nalini Desai , Shruti Jain , Daniel Tomso , Rong Guo , Julia Thissen
发明人： Nadine Carozzi , Nicholas Duck , Theodore Kahn , Nalini Desai , Shruti Jain , Daniel Tomso , Rong Guo , Julia Thissen
CPC分类号： C07K14/325 , C12N15/8285 , C12N15/8286 , Y02A40/162 , Y02A40/164
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, 4, 6, 8, 10, 12, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, or 38, or the nucleotide sequence set forth in SEQ ID NO: 1, 3, 5, 7, 9, 11, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, or 37, as well as variants and fragments thereof.
摘要翻译： 提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。 提供了包含δ-内毒素多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了分离的δ-内毒素核酸分子。 另外，包括对应于多核苷酸的氨基酸序列，以及与这些氨基酸序列特异性结合的抗体。 具体地说，本发明提供了分离的核酸分子，其包含编码SEQ ID NO： 27,29,31,33,35或38所示的核苷酸序列，或SEQ ID NO：1,3,5,7,9,11,14,16,18,20,22,24,26， 28，30，32，34或37，以及其变体和片段。
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