摘要:
Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for pesticidal polypeptides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated pesticidal nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO: 2, 4, 6, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37 or 61, the nucleotide sequence set forth in SEQ ID NO: 1, 3, 5, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, or 60, or the nucleotide sequence deposited in a bacterial host as Accession No. B-30955, B-30956, B-30957, B-30958, B-30942, B-30939, B-30941, B-50047, B-30959, B-30960, B-30943, or B-50048, as well as variants and fragments thereof.
摘要翻译:提供了赋予细菌,植物,植物细胞,组织和种子的杀虫活性的组合物和方法。 提供了包含杀虫多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中,用于在植物和细菌中转化和表达。 组合物还包括转化的细菌,植物,植物细胞,组织和种子。 特别地,提供了分离的杀虫核酸分子。 另外,包括对应于多核苷酸的氨基酸序列。 特别地,本发明提供了分离的核酸分子,其包含编码SEQ ID NO: 25,27,29,31,33,35,37或61所述的核苷酸序列,所述核苷酸序列如SEQ ID NO:1,3,5,8,10,12,14,16,18,20,22,24, 26,28,30,32,34,36或60个核苷酸序列,或作为保藏号B-30955,B-30956,B-30957,B-30958,B-30942,B- 30939,B-30941,B-50047,B-30959,B-30960,B-30943或B-50048,以及其变体和片段。
摘要:
Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, 4, 6, 8, 10, 12, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, or 38, or the nucleotide sequence set forth in SEQ ID NO:1, 3, 5, 7, 9, 11, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, or 37, as well as variants and fragments thereof.
摘要翻译:提供了赋予细菌,植物,植物细胞,组织和种子的杀虫活性的组合物和方法。 提供了包含δ-内毒素多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中,用于在植物和细菌中转化和表达。 组合物还包括转化的细菌,植物,植物细胞,组织和种子。 特别地,提供了分离的δ-内毒素核酸分子。 另外,包括对应于多核苷酸的氨基酸序列,以及与这些氨基酸序列特异性结合的抗体。 具体地说,本发明提供了分离的核酸分子,其包含编码SEQ ID NO: 27,29,31,33,35或38所示的核苷酸序列,或SEQ ID NO:1,3,5,7,9,11,14,16,18,20,22,24,26, 28,30,32,34或37,以及其变体和片段。
摘要:
A method and apparatus for the decapsulation of integrated circuit packages. The apparatus includes a support member, the support member having an open region and an adjustable device coupled to the support member. The adjustable device can be adapted to hold a BGA package such that a surface region of the BGA package is spatially disposed to face a decapsulation source and a plurality of balls on the BGA package remain free from contact from the decapsulation source and free from contact from a thermal source capable of causing damage to one or more of the balls. The decapsulation source is provided to subject a portion of the surface region of the BGA package for removal of the portion of the BGA package.
摘要:
Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, 4, 6, 8, 10, 12, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, or 38, or the nucleotide sequence set forth in SEQ ID NO: 1, 3, 5, 7, 9, 11, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, or 37, as well as variants and fragments thereof.
摘要翻译:提供了赋予细菌,植物,植物细胞,组织和种子的杀虫活性的组合物和方法。 提供了包含δ-内毒素多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中,用于在植物和细菌中转化和表达。 组合物还包括转化的细菌,植物,植物细胞,组织和种子。 特别地,提供了分离的δ-内毒素核酸分子。 另外,包括对应于多核苷酸的氨基酸序列,以及与这些氨基酸序列特异性结合的抗体。 具体地说,本发明提供了分离的核酸分子,其包含编码SEQ ID NO: 27,29,31,33,35或38所示的核苷酸序列,或SEQ ID NO:1,3,5,7,9,11,14,16,18,20,22,24,26, 28,30,32,34或37,以及其变体和片段。
摘要:
Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, 4, 6, 8, 10, 12, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, or 38, or the nucleotide sequence set forth in SEQ ID NO:1, 3, 5, 7, 9, 11, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, or 37, as well as variants and fragments thereof.
摘要翻译:提供了赋予细菌,植物,植物细胞,组织和种子的杀虫活性的组合物和方法。 提供了包含δ-内毒素多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中,用于在植物和细菌中转化和表达。 组合物还包括转化的细菌,植物,植物细胞,组织和种子。 特别地,提供了分离的δ-内毒素核酸分子。 另外,包括对应于多核苷酸的氨基酸序列,以及与这些氨基酸序列特异性结合的抗体。 具体地说,本发明提供了分离的核酸分子,其包含编码SEQ ID NO: 27,29,31,33,35或38所示的核苷酸序列,或SEQ ID NO:1,3,5,7,9,11,14,16,18,20,22,24,26, 28,30,32,34或37,以及其变体和片段。
摘要:
A method and apparatus for the decapsulation of integrated circuit packages. The apparatus includes a support member, the support member having an open region and an adjustable device coupled to the support member. The adjustable device can be adapted to hold a BGA package such that a surface region of the BGA package is spatially disposed to face a decapsulation source and a plurality of balls on the BGA package remain free from contact from the decapsulation source and free from contact from a thermal source capable of causing damage to one or more of the balls. The decapsulation source is provided to subject a portion of the surface region of the BGA package for removal of the portion of the BGA package.
摘要:
Compositions and methods for conferring insecticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions including a coding sequence for a Brevibacillus-derived delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules having nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, 4, 7, or 10, or the nucleotide sequence set forth in SEQ ID NO:1, 3, 5, 6, 8, 9, 11, 12, 13, 14, or 15, as well as variants and fragments thereof.
摘要翻译:提供了赋予细菌,植物,植物细胞,组织和种子的杀虫活性的组合物和方法。 提供了包含短杆菌属来源的δ-内毒素多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中,用于在植物和细菌中转化和表达。 组合物还包括转化的细菌,植物,植物细胞,组织和种子。 特别地,提供了分离的δ-内毒素核酸分子。 另外,包括对应于多核苷酸的氨基酸序列,以及与这些氨基酸序列特异性结合的抗体。 特别地,本发明提供了具有编码SEQ ID NO:2,4,7或10所示的氨基酸序列的核苷酸序列的分离的核酸分子或者SEQ ID NO: 5,6,8,9,11,12,13,14或15,以及其变体和片段。
摘要:
Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for pesticidal polypeptides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated pesticidal nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, 4, 6, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37 or 61, the nucleotide sequence set forth in SEQ ID NO:1, 3, 5, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, or 60, or the nucleotide sequence deposited in a bacterial host as Accession No. NRRL B-30961, B-30955, B-30956, B-30957, B-30958, B-30942, B-30939, B-30941, B-50047, B-30959, B-30960, B-30943, or B-50048, as well as variants and fragments thereof.
摘要翻译:提供了赋予细菌,植物,植物细胞,组织和种子的杀虫活性的组合物和方法。 提供了包含杀虫多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中,用于在植物和细菌中转化和表达。 组合物还包括转化的细菌,植物,植物细胞,组织和种子。 特别地,提供了分离的杀虫核酸分子。 另外,包括对应于多核苷酸的氨基酸序列。 特别地,本发明提供了分离的核酸分子,其包含编码SEQ ID NO: 25,27,29,31,33,35,37或61所述的核苷酸序列,所述核苷酸序列如SEQ ID NO:1,3,5,8,10,12,14,16,18,20,22,24, 26,28,30,32,34,36或60个核苷酸序列,或作为保藏号NRRL B-30961,B-30955,B-30956,B-30957,B-30958,B -30942,B-30939,B-30941,B-50047,B-30959,B-30960,B-30943或B-50048,以及其变体和片段。
摘要:
Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a toxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated toxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:28-62, or the nucleotide sequence set forth in SEQ ID NO:1-27, as well as variants and fragments thereof.
摘要翻译:提供了赋予细菌,植物,植物细胞,组织和种子的杀虫活性的组合物和方法。 提供了包含毒素多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中,用于在植物和细菌中转化和表达。 组合物还包括转化的细菌,植物,植物细胞,组织和种子。 特别地,提供了分离的毒素核酸分子。 另外,包括对应于多核苷酸的氨基酸序列,以及与这些氨基酸序列特异性结合的抗体。 特别地,本发明提供了分离的核酸分子,其包含编码SEQ ID NO:28-62所示氨基酸序列的核苷酸序列或SEQ ID NO:1-27所示的核苷酸序列,以及变体和 片段。
摘要:
Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for pesticidal polypeptides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated pesticidal nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, 4, 6, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37 or 61, the nucleotide sequence set forth in SEQ ID NO:1, 3, 5, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, or 60, or the nucleotide sequence deposited in a bacterial host as Accession No. NRRL B-30961, B-30955, B-30956, B-30957, B-30958, B-30942, B-30939, B-30941, B-50047, B-30959, B-30960, B-30943, or B-50048, as well as variants and fragments thereof.
摘要翻译:提供了赋予细菌,植物,植物细胞,组织和种子的杀虫活性的组合物和方法。 提供了包含杀虫多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中,用于在植物和细菌中转化和表达。 组合物还包括转化的细菌,植物,植物细胞,组织和种子。 特别地,提供了分离的杀虫核酸分子。 另外,包括对应于多核苷酸的氨基酸序列。 特别地,本发明提供了分离的核酸分子,其包含编码SEQ ID NO: 25,27,29,31,33,35,37或61所述的核苷酸序列,所述核苷酸序列如SEQ ID NO:1,3,5,8,10,12,14,16,18,20,22,24, 26,28,30,32,34,36或60个核苷酸序列,或作为保藏号NRRL B-30961,B-30955,B-30956,B-30957,B-30958,B -30942,B-30939,B-30941,B-50047,B-30959,B-30960,B-30943或B-50048,以及其变体和片段。