公开(公告)号：US08404934B2

公开(公告)日：2013-03-26

申请号：US12638591

申请日：2009-12-15

申请人： Cheryl L. Peters ,  Brian Vande Berg ,  Brian Carr ,  Julia T. Daum ,  Vadim Beilinson ,  Sandra Volrath ,  Candace Poutre ,  Kimberly Sampson

发明人： Cheryl L. Peters ,  Brian Vande Berg ,  Brian Carr ,  Julia T. Daum ,  Vadim Beilinson ,  Sandra Volrath ,  Candace Poutre ,  Kimberly Sampson

IPC分类号： C12N1/21 ,  C12N15/32 ,  C12N15/82 ,  A01N37/18 ,  C07K14/325

CPC分类号： C12N15/8241 ,  C12N9/0059 ,  C12N9/0071 ,  C12N15/8285 ,  C12Y110/03001 ,  C12Y114/18001 ,  Y02A40/164

摘要： Compositions and methods for conferring nematicidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions including a coding sequence for nematicidal polypeptides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated nematicidal nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules including nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:4, 5, 8, 9, 13, 14, 47, 48, or 49, the nucleotide sequence set forth in SEQ ID NO:1, 2, 3, 6, 7, 10, 11, 12, 15, 45, or 46, as well as variants and fragments thereof.

摘要翻译： 提供了赋予细菌，植物，植物细胞，组织和种子的杀线虫活性的组合物和方法。 提供了包含杀线虫多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了分离的杀线虫剂核酸分子。 另外，包含对应于多核苷酸的氨基酸序列。 特别地，本发明提供了分离的核酸分子，其包括编码SEQ ID NO：4,5,8,9,13,14,77,48或49所示氨基酸序列的核苷酸序列，所列出的核苷酸序列 在SEQ ID NO：1,2,3,6,7,10,11，12，15，45或46中，以及其变体和片段。

公开(公告)号：US5541310A

公开(公告)日：1996-07-30

申请号：US236427

申请日：1994-04-29

申请人： Eric R. Ward ,  Sandra Volrath ,  Shinichi Koizumi ,  Sachiyo Tada ,  Ichiro Mori ,  Genji Iwasaki

发明人： Eric R. Ward ,  Sandra Volrath ,  Shinichi Koizumi ,  Sachiyo Tada ,  Ichiro Mori ,  Genji Iwasaki

IPC分类号： A01H5/00 ,  C12N5/10 ,  C12N9/88 ,  C12N15/09 ,  C12N15/60 ,  C12N15/82 ,  C12Q1/527 ,  C12N15/29 ,  C12N15/63

CPC分类号： C12N9/88 ,  C12N15/8278 ,  C12Q1/527

摘要： Disclosed are plants, plant tissue and plant seed, whose growth and development are tolerant of, or resistant to various imidazole and triazole herbicidal compounds, at levels which normally are inhibitory to the plants. The tolerance or resistance is conferred by an altered imidazoleglycerol phosphate dehydratase (IGPD). Plant genes encoding wild-type and altered IGPD, purified plant IGPD, methods of isolating IGPD from plants, and methods of using both purified IGPD and IGPD-encoding genes are also disclosed.

摘要翻译： 公开了植物，植物组织和植物种子，其生长发育对各种咪唑和三唑除草化合物具有耐受性或耐受性，其水平通常对植物具有抑制作用。 耐受性或抗性由改变的咪唑磷酸脱水酶（IGPD）赋予。 还公开了编码野生型和改变的IGPD的植物基因，纯化植物IGPD，从植物分离IGPD的方法，以及使用纯化的IGPD和IGPD编码基因的方法。

公开(公告)号：US20090144852A1

公开(公告)日：2009-06-04

申请号：US12252453

申请日：2008-10-16

申请人： Daniel J. Tomso ,  Nalini Desai ,  Shruti Agarwal ,  Kimberly S. Sampson ,  Sandra Volrath ,  Volker Heinrichs

发明人： Daniel J. Tomso ,  Nalini Desai ,  Shruti Agarwal ,  Kimberly S. Sampson ,  Sandra Volrath ,  Volker Heinrichs

IPC分类号： C12N15/82 ,  A01H5/00 ,  C12N15/11 ,  C12N15/00 ,  C12N1/21 ,  C12N5/04 ,  C07K14/00 ,  A61K38/16 ,  C12P21/02

CPC分类号： C07K14/325 ,  C12N15/8285 ,  C12N15/8286 ,  Y02A40/162 ,  Y02A40/164

摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for pesticidal polypeptides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated pesticidal nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:5, 2, or 10, the nucleotide sequence set forth in SEQ ID NO:4, 1, 3, 4, 6, 9, or 11, or the nucleotide sequence deposited in a bacterial host as Accession No. B-50045, as well as variants and fragments thereof.

摘要翻译： 提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。 提供了包含杀虫多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了分离的杀虫核酸分子。 另外，包括对应于多核苷酸的氨基酸序列。 特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：5,2或10所示氨基酸序列的核苷酸序列，SEQ ID NO：4,1,3,4 ，6,9或11个核苷酸序列，或以细菌宿主保藏号B-50045以及其变体和片段沉积的核苷酸序列。

公开(公告)号：US06177245B1

公开(公告)日：2001-01-23

申请号：US09071296

申请日：1998-05-01

申请人： Eric R. Ward ,  Sandra Volrath

发明人： Eric R. Ward ,  Sandra Volrath

IPC分类号： C12Q168

CPC分类号： C12Y103/03004 ,  C12N5/04 ,  C12N9/001 ,  C12N15/8274 ,  C12Q1/6895

摘要： The present invention provides novel eukaryotic DNA sequences coding for native protoporphyrinogen oxidase (protox) or modified forms of the enzyme which are herbicide tolerant. Plants having altered protox activity which confers tolerance to herbicides are also provided. These plants may be bred or engineered for resistance to protox inhibitors via mutation of the native protox gene to a resistant form or through increased levels of expression of the native protox gene, or they may be transformed with modified eukaryotic or prokaryotic protox coding sequences or wild type prokaryotic protox sequences which are herbicide tolerant. Diagnostic and other uses for the novel eukaryotic protox sequence are also described. Plant genes encoding wild-type and altered protox, purified plant protox, methods of isolating protox from plants, and methods of using protox-encoding genes are also disclosed.

摘要翻译： 本发明提供编码天然原卟啉原氧化酶（protox）或耐除草剂的酶的修饰形式的新型真核DNA序列。 还提供了赋予除草剂耐受性的具有改变的排毒活性的植物。 这些植物可以通过将天然protox基因突变为抗性形式或通过增加天然protox基因的表达水平培育或设计用于抵抗前毒素抑制剂，或者可以用经修饰的真核或原核protox编码序列或野生型 类型的原核生殖毒素序列是耐除草剂。 还描述了用于新型真核毒素序列的诊断和其它用途。 还公开了编码野生型和改变的protox的植物基因，纯化的植物protox，从植物中分离protox的方法，以及使用protox编码基因的方法。

公开(公告)号：US06282837B1

公开(公告)日：2001-09-04

申请号：US09196268

申请日：1998-11-19

申请人： Eric R. Ward ,  Sandra Volrath

发明人： Eric R. Ward ,  Sandra Volrath

IPC分类号： A01N2500

CPC分类号： C12Y103/03004 ,  C12N5/04 ,  C12N9/001 ,  C12N15/8274 ,  C12Q1/6895

摘要： The present invention provides novel eukaryotic DNA sequences coding for native protoporphyrinogen oxidase (protox) or modified forms of the enzyme which are herbicide tolerant. A method for controlling weeds using plants having altered protox activity which confers tolerance to herbicides is described.

摘要翻译： 本发明提供编码天然原卟啉原氧化酶（protox）或耐除草剂的酶的修饰形式的新型真核DNA序列。 描述了使用赋予对除草剂耐受性的具有改变的排毒活性的植物来控制杂草的方法。

公开(公告)号：US20050081259A1

公开(公告)日：2005-04-14

申请号：US10625648

申请日：2003-07-23

申请人： Peter Heifetz ,  Sandra Volrath ,  Marie Johnson ,  Eric Ward

发明人： Peter Heifetz ,  Sandra Volrath ,  Marie Johnson ,  Eric Ward

IPC分类号： C12N5/04 ,  C12N9/02 ,  C12N15/82 ,  C07H21/04 ,  A01H1/00

CPC分类号： C12Y103/03004 ,  C12N5/04 ,  C12N9/001 ,  C12N15/8214 ,  C12N15/8216 ,  C12N15/8223 ,  C12N15/8274

摘要： The present invention provides novel DNA sequences coding for protoporphyrinogen oxidase (protox) enzymes from soybean, wheat, cotton, sugar beet, oilseed rape, rice, sorghum, and sugar cane. In addition, the present invention teaches modified forms of protox enzymes that are herbicide tolerant. Plants expressing herbicide tolerant protox enzymes taught herein are also provided. These plants may be engineered for resistance to protox inhibitors via mutation of the native protox gene to a resistant form or they may be transformed with a gene encoding an herbicide tolerant form of a plant protox enzyme. The present invention further provides shuffled DNA molecules encoding protox enzymes having enhanced tolerance to a herbicide that inhibits the protox activity encoded by a template DNA molecule from which the shuffled DNA molecule is derived.

摘要翻译： 本发明提供了编码大豆，小麦，棉花，甜菜，油菜，稻，高粱和甘蔗的原卟啉原氧化酶（protox）酶的新型DNA序列。 此外，本发明教导了耐除草剂的脱毒酶的修饰形式。 还提供了表达本文教导的除草剂耐受性前体毒素酶的植物。 这些植物可以通过将天然protox基因突变为抗性形式而被设计用于抵抗前毒素抑制剂，或者它们可以用编码除草剂耐受形式的植物泌毒素酶的基因转化。 本发明进一步提供编码具有增强的对除草剂的耐受性的排毒酶的洗牌DNA分子，所述除草剂抑制由洗牌的DNA分子衍生的模板DNA分子编码的前导毒素活性。

公开(公告)号：US5767373A

公开(公告)日：1998-06-16

申请号：US472028

申请日：1995-06-06

申请人： Eric R. Ward ,  Sandra Volrath

发明人： Eric R. Ward ,  Sandra Volrath

IPC分类号： G01N33/566 ,  A01H1/04 ,  A01H5/00 ,  A01N43/38 ,  A01N43/54 ,  A01N43/56 ,  A01N43/653 ,  A01N43/824 ,  A01N43/836 ,  A01N43/84 ,  A61K38/44 ,  A61K48/00 ,  A61P43/00 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/04 ,  C12N5/10 ,  C12N5/14 ,  C12N9/02 ,  C12N15/09 ,  C12N15/53 ,  C12N15/82 ,  C12Q1/26 ,  C12Q1/68 ,  C12R1/91 ,  C12N15/00

CPC分类号： C12Y103/03004 ,  C12N15/8274 ,  C12N5/04 ,  C12N9/001 ,  C12Q1/6895

摘要： The present invention provides novel eukaryotic DNA sequences coding for native protoporphyrinogen oxidase (protox) or modified forms of the enzyme which are herbicide tolerant. Plants having altered protox activity which confers tolerance to herbicides are also provided. These plants may be bred or engineered for resistance to protox inhibitors via mutation of the native protox gene to a resistant form or through increased levels of expression of the native protox gene, or they may be transformed with modified eukaryotic or prokaryotic protox coding sequences or wild type prokaryotic protox sequences which are herbicide tolerant. Diagnostic and other uses for the novel eukaryotic protox sequence are also described. Plant genes encoding wild-type and altered protox, purified plant protox, methods of isolating protox from plants, and methods of using protox-encoding genes are also disclosed.

摘要翻译： 本发明提供编码天然原卟啉原氧化酶（protox）或耐除草剂的酶的修饰形式的新型真核DNA序列。 还提供了赋予除草剂耐受性的具有改变的排毒活性的植物。 这些植物可以通过将天然protox基因突变为抗性形式或通过增加天然protox基因的表达水平培育或设计用于抵抗前毒素抑制剂，或者可以用经修饰的真核或原核protox编码序列或野生型 类型的原核生殖毒素序列是耐除草剂。 还描述了用于新型真核毒素序列的诊断和其它用途。 还公开了编码野生型和改变的protox的植物基因，纯化的植物protox，从植物中分离protox的方法，以及使用protox编码基因的方法。

公开(公告)号：US20100166723A1

公开(公告)日：2010-07-01

申请号：US12638591

申请日：2009-12-15

申请人： Cheryl L. Peters ,  Brian Vande Berg ,  Brian Carr ,  Julia T. Daum ,  Vadim Beilinson ,  Sandra Volrath ,  Candace Poutre ,  Kimberly Sampson ,  Theodore Kahn

发明人： Cheryl L. Peters ,  Brian Vande Berg ,  Brian Carr ,  Julia T. Daum ,  Vadim Beilinson ,  Sandra Volrath ,  Candace Poutre ,  Kimberly Sampson ,  Theodore Kahn

IPC分类号： A61K38/44 ,  A01H5/00 ,  C12N15/82 ,  C07H21/04 ,  C12N15/63 ,  C12N1/21 ,  C12N5/10 ,  C12N9/02

CPC分类号： C12N15/8241 ,  C12N9/0059 ,  C12N9/0071 ,  C12N15/8285 ,  C12Y110/03001 ,  C12Y114/18001 ,  Y02A40/164

摘要： Compositions and methods for conferring nematicidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions including a coding sequence for nematicidal polypeptides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated nematicidal nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules including nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:4, 5, 8, 9, 13, 14, 47, 48, or 49, the nucleotide sequence set forth in SEQ ID NO:1, 2, 3, 6, 7, 10, 11, 12, 15, 45, or 46, as well as variants and fragments thereof.

摘要翻译： 提供了赋予细菌，植物，植物细胞，组织和种子的杀线虫活性的组合物和方法。 提供了包含杀线虫多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了分离的杀线虫剂核酸分子。 另外，包括对应于多核苷酸的氨基酸序列。 特别地，本发明提供了分离的核酸分子，其包括编码SEQ ID NO：4,5,8,9,13,14,77,48或49所示氨基酸序列的核苷酸序列，所列出的核苷酸序列 在SEQ ID NO：1,2,3,6,7,10,11，12，15，45或46中，以及其变体和片段。

公开(公告)号：US06307129B1

公开(公告)日：2001-10-23

申请号：US09191998

申请日：1998-11-12

申请人： Eric R. Ward ,  Sandra Volrath

发明人： Eric R. Ward ,  Sandra Volrath

IPC分类号： A01H500

CPC分类号： C12Y103/03004 ,  C12N5/04 ,  C12N9/001 ,  C12N15/8274 ,  C12Q1/6895

摘要： The present invention provides novel eukaryotic DNA sequences coding for native protoporphyrinogen oxidase (protox) or modified forms of the enzyme which are herbicide tolerant. Plants having altered protox activity which confers tolerance to herbicides are also provided. These plants are engineered for resistance to protox inhibitors via mutation of the native protox gene to a resistant form or they are transformed with modified eukaryotic or prokaryotic protox coding sequences or wild type prokaryotic protox sequences which are herbicide tolerant. Plant genes encoding wild-type and altered protox, purified plant protox, methods of isolating protox from plants, and methods of using protox-encoding genes are also disclosed.

摘要翻译： 本发明提供编码天然原卟啉原氧化酶（protox）或耐除草剂的酶的修饰形式的新型真核DNA序列。 还提供了赋予除草剂耐受性的具有改变的排毒活性的植物。 这些植物通过将天然protox基因突变为抗性形式而被设计用于抵抗前毒素抑制剂，或者用修饰的真核或原核protox编码序列或耐除草剂的野生型原核protox序列转化。 还公开了编码野生型和改变的protox的植物基因，纯化的植物protox，从植物中分离protox的方法，以及使用protox编码基因的方法。

公开(公告)号：US06288306B1

公开(公告)日：2001-09-11

申请号：US09015683

申请日：1998-01-29

申请人： Eric R. Ward ,  Sandra Volrath

发明人： Eric R. Ward ,  Sandra Volrath

IPC分类号： A01H500

CPC分类号： C12Y103/03004 ,  C12N5/04 ,  C12N9/001 ,  C12N15/8274 ,  C12Q1/6895

摘要： The present invention provides novel eukaryotic DNA sequences coding for native protoporphyrinogen oxidase (protox) or modified forms of the enzyme which are herbicide tolerant. Plants having altered protox activity which confers tolerance to herbicides and a method of selecting transformed plants are also provided.

摘要翻译： 本发明提供编码天然原卟啉原氧化酶（protox）或耐除草剂的酶的修饰形式的新型真核DNA序列。 还提供了赋予对除草剂的耐受性的改变的排毒活性的植物和选择转化植物的方法。