公开(公告)号：US20070294787A1

公开(公告)日：2007-12-20

申请号：US11762886

申请日：2007-06-14

申请人： Nadine Carozzi ,  Nicholas Duck ,  Theodore Kahn ,  Nalini Desai ,  Shruti Jain ,  Daniel Tomso ,  Rong Guo ,  Julia Thissen

发明人： Nadine Carozzi ,  Nicholas Duck ,  Theodore Kahn ,  Nalini Desai ,  Shruti Jain ,  Daniel Tomso ,  Rong Guo ,  Julia Thissen

IPC分类号： A01H1/00 ,  C07H21/04 ,  C12N15/82 ,  A01N65/00 ,  C12N5/04

CPC分类号： C07K14/325 ,  C12N15/8285 ,  C12N15/8286 ,  Y02A40/162 ,  Y02A40/164

摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, 4, 6, 8, 10, 12, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, or 38, or the nucleotide sequence set forth in SEQ ID NO: 1, 3, 5, 7, 9, 11, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, or 37, as well as variants and fragments thereof.

摘要翻译： 提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。 提供了包含δ-内毒素多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了分离的δ-内毒素核酸分子。 另外，包括对应于多核苷酸的氨基酸序列，以及与这些氨基酸序列特异性结合的抗体。 具体地说，本发明提供了分离的核酸分子，其包含编码SEQ ID NO： 27,29,31,33,35或38所示的核苷酸序列，或SEQ ID NO：1,3,5,7,9,11,14,16,18,20,22,24,26， 28，30，32，34或37，以及其变体和片段。

公开(公告)号：US08759619B2

公开(公告)日：2014-06-24

申请号：US13030399

申请日：2011-02-18

申请人： Kimberly Sampson ,  Daniel Tomso

发明人： Kimberly Sampson ,  Daniel Tomso

IPC分类号： A01H5/00 ,  C12N15/82 ,  C12P1/00 ,  C07K1/00 ,  C07H21/02

CPC分类号： C07K14/325 ,  A01N37/46 ,  A01N63/02 ,  C12N15/8285 ,  C12N15/8286 ,  Y02A40/162 ,  Y02A40/164

摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a toxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated toxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:13-30, or the nucleotide sequence set forth in SEQ ID NO:1-12, as well as variants and fragments thereof.

摘要翻译： 提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。 提供了包含毒素多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了分离的毒素核酸分子。 另外，包括对应于多核苷酸的氨基酸序列，以及与这些氨基酸序列特异性结合的抗体。 特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：13-30所示的氨基酸序列的核苷酸序列或SEQ ID NO：1-12所示的核苷酸序列，以及变体和 片段。

公开(公告)号：US20070289035A1

公开(公告)日：2007-12-13

申请号：US11760570

申请日：2007-06-08

申请人： Brian Vande Berg ,  Cheryl Peters ,  Brian Carr ,  Daniel Tomso

发明人： Brian Vande Berg ,  Cheryl Peters ,  Brian Carr ,  Daniel Tomso

IPC分类号： A01H5/00 ,  C12N15/82

CPC分类号： C12N15/8275 ,  C12N9/1092 ,  C12N15/8261

摘要： Compositions and methods for conferring herbicide resistance or tolerance to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a polypeptide that confers resistance or tolerance to glyphosate herbicides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated nucleic acid molecules corresponding to glyphosate resistant nucleic acid sequences are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NOS:2, 4, 6, 8, 10, 12, or 14 or the nucleotide sequence set forth in SEQ ID NOS:1, 3, 5, 7, 9, 11, 13, 28, 29, 30, 31, 32, 33, or 34.

摘要翻译： 提供了赋予除草剂抗性或对细菌，植物，植物细胞，组织和种子的耐受性的组合物和方法。 提供了包含赋予对草甘膦除草剂的抗性或耐受性的多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中用于在植物中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了与草甘膦抗性核酸序列相对应的分离的核酸分子。 另外，包括对应于多核苷酸的氨基酸序列。 特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：2,4,6,8,10,12或14所示氨基酸序列的核苷酸序列或SEQ ID NOS ：1，3，5，7，9，11，13，28，29，30，31，32，33或34。

公开(公告)号：US08686124B2

公开(公告)日：2014-04-01

申请号：US13030415

申请日：2011-02-18

申请人： Kimberly Sampson ,  Daniel Tomso

发明人： Kimberly Sampson ,  Daniel Tomso

IPC分类号： A01H5/00 ,  C12N15/82 ,  C12N15/00 ,  C12N5/00 ,  C12P21/00 ,  A01N63/00

CPC分类号： C12N15/8286 ,  A01N37/46 ,  A01N63/02 ,  C07K14/325 ,  C07K16/1278 ,  C12N15/8279 ,  Y02A40/162

摘要： Compositions and methods for conferring pesticidal activity, including against lepidopteran pests, to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a toxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated toxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:27 and 28, or the nucleotide sequence set forth in SEQ ID NO:3, 8, 13, and 18, as well as variants and fragments thereof.

摘要翻译： 提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性（包括防鳞翅目害虫）的组合物和方法。 提供了包含毒素多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了分离的毒素核酸分子。 另外，包括对应于多核苷酸的氨基酸序列，以及与这些氨基酸序列特异性结合的抗体。 特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：27和28所示的氨基酸序列的核苷酸序列或SEQ ID NO：3,8,13和18所示的核苷酸序列， 以及其变体和片段。

公开(公告)号：US20110203015A1

公开(公告)日：2011-08-18

申请号：US13030415

申请日：2011-02-18

申请人： Kimberly Sampson ,  Daniel Tomso

发明人： Kimberly Sampson ,  Daniel Tomso

IPC分类号： A01H5/00 ,  C07H21/04 ,  C12N15/63 ,  C12N1/21 ,  C12N5/10 ,  C07K14/00 ,  A01N37/18 ,  C12P21/00 ,  A01H1/00 ,  C12N15/82 ,  A01H5/10 ,  A01P7/04

CPC分类号： C12N15/8286 ,  A01N37/46 ,  A01N63/02 ,  C07K14/325 ,  C07K16/1278 ,  C12N15/8279 ,  Y02A40/162

摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a toxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated toxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:21-32, or the nucleotide sequence set forth in SEQ ID NO:1-20, as well as variants and fragments thereof.

摘要翻译： 提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。 提供了包含毒素多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了分离的毒素核酸分子。 另外，包括对应于多核苷酸的氨基酸序列，以及与这些氨基酸序列特异性结合的抗体。 特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：21-32所示的氨基酸序列的核苷酸序列或SEQ ID NO：1-20所示的核苷酸序列，以及变体和 片段。

公开(公告)号：US20110203014A1

公开(公告)日：2011-08-18

申请号：US13030399

申请日：2011-02-18

申请人： Kimberly Sampson ,  Daniel Tomso

发明人： Kimberly Sampson ,  Daniel Tomso

IPC分类号： A01H5/00 ,  C07H21/04 ,  C12N15/63 ,  C12N1/21 ,  C12N5/10 ,  C07K14/00 ,  A01N37/18 ,  C12P21/00 ,  A01H1/00 ,  C12N15/82 ,  A01H5/10 ,  A01P7/04

CPC分类号： C07K14/325 ,  A01N37/46 ,  A01N63/02 ,  C12N15/8285 ,  C12N15/8286 ,  Y02A40/162 ,  Y02A40/164

摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a toxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated toxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:13-30, or the nucleotide sequence set forth in SEQ ID NO:1-12, as well as variants and fragments thereof.

摘要翻译： 提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。 提供了包含毒素多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了分离的毒素核酸分子。 另外，包括对应于多核苷酸的氨基酸序列，以及与这些氨基酸序列特异性结合的抗体。 特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：13-30所示的氨基酸序列的核苷酸序列或SEQ ID NO：1-12所示的核苷酸序列，以及变体和 片段。

公开(公告)号：US20080070829A1

公开(公告)日：2008-03-20

申请号：US11763947

申请日：2007-06-15

申请人： Nadine Carozzi ,  Michael Koziel ,  Nicholas Duck ,  Nalini Desai ,  Rong Guo ,  Daniel Tomso ,  Rebekah Deter ,  Tracy Hargiss

发明人： Nadine Carozzi ,  Michael Koziel ,  Nicholas Duck ,  Nalini Desai ,  Rong Guo ,  Daniel Tomso ,  Rebekah Deter ,  Tracy Hargiss

IPC分类号： A01N37/18 ,  A01H1/00 ,  A01H5/00 ,  A01P5/00 ,  C12N1/00 ,  C12N15/63 ,  C12P21/00 ,  C12N5/04 ,  C12N1/21 ,  C07H21/00 ,  A01P15/00 ,  A01H5/10

CPC分类号： C12N15/8286 ,  A01N37/44 ,  C07K14/325 ,  C12P21/02 ,  Y02A40/162

摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for pesticidal polypeptides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated pesticidal nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, 4, 6, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37 or 61, the nucleotide sequence set forth in SEQ ID NO:1, 3, 5, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, or 60, or the nucleotide sequence deposited in a bacterial host as Accession No. NRRL B-30961, B-30955, B-30956, B-30957, B-30958, B-30942, B-30939, B-30941, B-50047, B-50047, B-30959, B-30960, B-30943, B-50048, or B-50048, as well as variants and fragments thereof.

摘要翻译： 提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。 提供了包含杀虫多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了分离的杀虫核酸分子。 另外，包括对应于多核苷酸的氨基酸序列。 特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO： 25，27，29，31，33，35，37或61所述的核苷酸序列，所述核苷酸序列如SEQ ID NO：1,3,5,8,10,12,14,16,18,20,22,24， 26,28,30,32,34,36或60个核苷酸序列，或作为保藏号NRRL B-30961，B-30955，B-30956，B-30957，B-30958，B -30942，B-30939，B-30941，B-50047，B-50047，B-30959，B-30960，B-30943，B-50048或B-50048，以及其变体和片段。