公开(公告)号：US07135540B2

公开(公告)日：2006-11-14

申请号：US10359600

申请日：2003-02-07

申请人： Tsutomu Honma ,  Shinya Kozaki ,  Takeshi Imamura ,  Takashi Kenmoku ,  Tatsuki Fukui ,  Etsuko Sugawa ,  Tetsuya Yano

发明人： Tsutomu Honma ,  Shinya Kozaki ,  Takeshi Imamura ,  Takashi Kenmoku ,  Tatsuki Fukui ,  Etsuko Sugawa ,  Tetsuya Yano

IPC分类号： C08G63/02

CPC分类号： C12P7/625 ,  C08G63/6822 ,  C08G63/6882 ,  C12P11/00

摘要： The invention provides a polyhydroxyalkanoate having a bromo group in a unit and being thermally stable and capable of arbitrarily controlling physical properties, and a producing method thereof. According to the invention, there are provided a polyhydroxyalkanoate copolymer including a 3-hydroxy-ω-bromoalkanoic acid unit represented by a formula—[OCH((CH2)nBr)CH2C(O)]— (n=1 to 8) (1) and a unit represented by a formula —[OCH((CH2)mR)CH2C(O)]— (2) within a same molecule, and a method for producing a polyhydroxyalkanoate copolymer by microorganisms, utilizing a ω-bromoalkanoic acid represented by a formula Br(CH2)pCH2CH2COOH (p=1 to 8) (20) and a compound represented by a formula R(CH2)qCH2CH2COOH (21) as raw materials.

摘要翻译： 本发明提供一种具有溴基团并且是热稳定的并能够任意控制物理性能的聚羟基链烷酸酯及其制备方法。 根据本发明，提供了一种包含由式 - [OCH（（CH 2）n）n Br表示的3-羟基-ω-溴代链烷酸单元的聚羟基链烷酸酯共聚物 ）CH 2 C（O）] - （n = 1-8）（1）和由式 - [OCH（（CH 2）2） （O）] - （2），以及通过微生物制备聚羟基链烷酸酯共聚物的方法，使用由以下化合物表示的ω-溴烷酸： 式（Br 2 CH 2 CH 2 CH 2 CH 2 CH 2 CH 2 CH 2 COOH（p = 1〜8）（20 ）和由式R（CH 2 CH 2）q CH 2 CH 2 COOH（21）表示的化合物 作为原料。

公开(公告)号：US20060040366A1

公开(公告)日：2006-02-23

申请号：US10531572

申请日：2003-10-23

申请人： Takashi Kenmoku ,  Tetsuya Yano ,  Shinya Kozaki ,  Tsutomu Honma

发明人： Takashi Kenmoku ,  Tetsuya Yano ,  Shinya Kozaki ,  Tsutomu Honma

IPC分类号： C12P7/62 ,  C08G69/44

CPC分类号： C12P7/625 ,  C08G63/06 ,  C08G63/664 ,  C08G63/6882

摘要： Provided are a PHA having an active group, which is produced by microorganisms with high productivity, and in which the ratio of units on its side chain having an active group can be controlled and the physical properties can be arbitrarily controlled so that its application as a polymer is not limited, and a method for producing the same. A 3-hydroxy-ω-[(phenylmethyl)oxy]alkanoic acid unit expressed by the following chemical formula (1): wherein x can be one or more integers within the range shown in the chemical formula.

摘要翻译： 提供具有活性基团的PHA，其由高生产率的微生物产生，并且其中可以控制具有活性基团的侧链上的单元比例，并且物理性质可以任意控制，使得其作为 聚合物不受限制，也可以是其制造方法。 由以下化学式（1）表示的3-羟基-ω（ - （（苯甲基）氧基）链烷酸单元：其中x可以是化学式所示范围内的一个或多个整数。

公开(公告)号：US20050196521A1

公开(公告)日：2005-09-08

申请号：US11062816

申请日：2005-02-23

申请人： Shinya Kozaki ,  Tsutomu Honma ,  Tetsuya Yano

发明人： Shinya Kozaki ,  Tsutomu Honma ,  Tetsuya Yano

IPC分类号： B41J2/01 ,  A61L2/00 ,  C12N15/09 ,  C12P7/62

CPC分类号： C12P7/625 ,  G01N33/54353

摘要： A process for forming a fine pattern comprised of a polyhydroxyalkanoate on a substrate is provided. The process comprises steps of applying a polyhydroxyalkanoate-synthesizing enzyme and a 3-hydroxyacyl CoA to coexist in an intended pattern on a substrate, and polymerizing the 3-hydroxyalkanoate by action of the enzyme to form the polyhydroxyalkanoate in a pattern on the substrate. In particular, the fine pattern can be formed by an inkjet system.

摘要翻译： 提供了在基材上形成由聚羟基链烷酸酯构成的精细图案的方法。 该方法包括将聚羟基链烷酸酯合成酶和3-羟基酰基CoA以预期图案共存于底物上的步骤，并通过酶的作用聚合3-羟基链烷酸酯以在基底上形成图案的聚羟基链烷酸酯。 特别地，精细图案可以由喷墨系统形成。

公开(公告)号：US06660516B1

公开(公告)日：2003-12-09

申请号：US09025424

申请日：1998-02-18

申请人： Takeshi Imamura ,  Yasutsugu Yamada ,  Tetsuya Yano ,  Akira Kuriyama ,  Shinya Kozaki

发明人： Takeshi Imamura ,  Yasutsugu Yamada ,  Tetsuya Yano ,  Akira Kuriyama ,  Shinya Kozaki

IPC分类号： B09B300

CPC分类号： C12N1/38 ,  C02F1/4618 ,  C02F3/34 ,  C02F2209/04 ,  C02F2209/06 ,  C12R1/19 ,  Y10S435/822 ,  Y10S435/849

摘要： A method is provided for culturing a microorganism in a culture medium containing a carbon source and an electrolyzed water containing not more than 0.4 ppm chlorine produced during electrolyzing water containing an electrolyte in an electrolytic cell. The culture medium may also contain a carbon source and an acidic water having a pH value of 1-4 and a redox potential from 800 mV to 1500 mV. The acidic water is obtained by electrolysis of water in an electrolytic cell. Further, the culture medium may contain a carbon source and an alkaline water having a pH value of 10-13 and a redox potential from −1000 mV to 800 mV. The alkaline water is obtained by electrolysis of water in an electrolytic cell. The redox potential for the alkaline water is determined by the use of a platinum electrode as a working electrode and a silver-silver chloride electrode as a reference electrode. The microorganism may be selected from Escherichia coli, strain J1, strain JM2N, or an artificial recombinant. The microorganism can decompose a pollutant, such as phenol, toluene, cresol, trichlorethylene and dichloroethylene.

摘要翻译： 提供了一种在含有碳源的培养基中培养微生物的方法，以及在电解槽中含有电解质中含有电解质的水中产生的含有不超过0.4ppm氯的电解水。 培养基也可以含有碳源和pH值为1-4，氧化还原电位为800mV〜1500mV的酸性水。 酸性水通过在电解池中电解水而获得。 此外，培养基可以含有碳源和pH值为10-13，氧化还原电位为-1000mV至800mV的碱性水。 碱性水通过在电解槽中电解电解而获得。 碱性水的氧化还原电位通过使用铂电极作为工作电极和银 - 氯化银电极作为参比电极来确定。 微生物可以选自大肠杆菌，菌株J1，菌株JM2N，或人工重组体。 微生物可以分解苯酚，甲苯，甲酚，三氯乙烯和二氯乙烯等污染物。

公开(公告)号：US07615233B2

公开(公告)日：2009-11-10

申请号：US10191563

申请日：2002-07-10

申请人： Tetsuya Yano ,  Tsuyoshi Nomoto ,  Shinya Kozaki ,  Tsutomu Honma

发明人： Tetsuya Yano ,  Tsuyoshi Nomoto ,  Shinya Kozaki ,  Tsutomu Honma

IPC分类号： A01N25/34

CPC分类号： A61K8/11 ,  A61K8/35 ,  A61K9/5031 ,  A61K47/6925 ,  A61K49/223 ,  A61K2800/412 ,  A61Q17/04 ,  B01J13/04 ,  B01J13/06 ,  B01J13/14 ,  Y02A50/473

摘要： The invention provides a particulate construct comprising a polyhydroxyalkanoate to serve as microcapsules containing a drug, and serving a slow releasing preparation not associated with a practically unacceptable initial burst release but showing a practically acceptable zero-order release for a certain period and a producing method for such particulate construct, and a slow releasing preparation of a high drug content capable of stably incorporating the drug in the particulate construct such as microcapsules, and a producing method for such preparation.

摘要翻译： 本发明提供了一种包含聚羟基链烷酸酯的颗粒构造物，用作含有药物的微胶囊，并且用于与实际上不可接受的初始爆发释放无关的缓释制剂，但在一定时间内显示出实际可接受的零级释放， 这种颗粒构造物，以及能够稳定地将药物并入微粒构造物如微胶囊中的高药物含量的缓释制剂及其制备方法。

公开(公告)号：US07527809B2

公开(公告)日：2009-05-05

申请号：US10553221

申请日：2004-04-30

申请人： Tetsuya Yano ,  Tsuyoshi Nomoto ,  Shinya Kozaki ,  Takeshi Imamura ,  Tsutomu Honma

发明人： Tetsuya Yano ,  Tsuyoshi Nomoto ,  Shinya Kozaki ,  Takeshi Imamura ,  Tsutomu Honma

IPC分类号： A61K9/14 ,  A61K9/50

CPC分类号： A61K9/5094 ,  A61K49/226 ,  Y10T428/12014

摘要： A structure containing polyhydroxyalkanoate and a magnetic substance includes an external phase part containing polyhydroxyalkanoate and an internal phase part contained in the external phase part, at least one of the external phase part and the internal phase part containing a magnetic substance. Accordingly, provided is a microcapsule, which can be suitably used for formation of an active ingredient of a sustained release pharmaceutical preparation or an ultrasonic contrast agent.

摘要翻译： 包含聚羟基链烷酸酯和磁性物质的结构包括含有聚羟基链烷酸酯的外相部分和包含在外部相部分中的内相部分，外相部分和内相部分中的至少一个含有磁性物质。 因此，提供了一种微胶囊，其可适用于形成持续释放药物制剂或超声造影剂的活性成分。

公开(公告)号：US07351786B2

公开(公告)日：2008-04-01

申请号：US10692206

申请日：2003-10-22

申请人： Tsutomu Honma ,  Tetsuya Yano ,  Shinya Kozaki

发明人： Tsutomu Honma ,  Tetsuya Yano ,  Shinya Kozaki

IPC分类号： C08G63/06 ,  C00G63/137

CPC分类号： C08G63/6882 ,  C08G63/06 ,  C12P7/625

摘要： Disclosed are a polyhydroxyalkanoate, containing in a polymer molecule thereof a monomer unit represented by the following general formula (1): (wherein R1 represents a substituent to a cyclohexyl group, where R1 is a H atom, a CN group, a NO2 group, a halogen atom, a CH3 group, a C2H5 group, a C3H7 group, a CF3 group, a C2F5 group, or a C3F7 group; and if there are two or more monomer units, R1 may be different from every monomer unit), and a binder resin using the polyhydroxyalkanoate. The binder resin is biodegradable, so that the binder resin is more responsible for the conservation of natural environment or the like. Also, the binder resin facilitates deinking in the deinking process using an alkali, so that the binder resin accelerates recycling of used copying paper. Furthermore, the binder resin satisfies the desired characteristics of a toner for developing an electrostatic charge image.

摘要翻译： 公开了一种聚羟基链烷酸酯，其聚合物分子中含有下列通式（1）表示的单体单元：（其中R 1表示与环己基的取代基，其中R 1为H原子，CN基团，NO _{2个基团，卤素原子，CH 3 3基团，C 2 H 5 H基团，C 1 -C 4亚烷基， 3个H 7基团，CF 3 N基团，C 2 H 5 F 5基团，或 C 3 N 7 F 7基团;如果有两个或多个单体单元，则R1可以不同于每个单体单元）和使用聚羟基链烷酸酯的粘合剂树脂。 粘合剂树脂是可生物降解的，使得粘合剂树脂对于保护自然环境等更有责任感。 此外，粘合剂树脂有助于使用碱的脱墨工艺中的脱墨，使得粘合剂树脂加速废旧复印纸的再循环。 此外，粘合剂树脂满足用于显影静电荷图像的调色剂的期望特性。}

公开(公告)号：US20060287473A1

公开(公告)日：2006-12-21

申请号：US11172662

申请日：2005-06-24

申请人： Tatsuki Fukui ,  Tetsuya Yano ,  Chieko Mihara ,  Shinya Kozaki ,  Tsutomu Honma ,  Takashi Kenmoku ,  Ako Kusakari

发明人： Tatsuki Fukui ,  Tetsuya Yano ,  Chieko Mihara ,  Shinya Kozaki ,  Tsutomu Honma ,  Takashi Kenmoku ,  Ako Kusakari

IPC分类号： C08G63/02

CPC分类号： C08G63/912 ,  C08G63/6852 ,  C08G63/6882 ,  G03G9/08755 ,  G03G9/08791

摘要： The invention provides a novel polyester adapted for use in a charge control agent for a toner for an electrophotographic process, having a charge stability, a high charge amount, an improved dispersibility and a biodegradability, and a producing method and an application technology therefor. According to the invention, there is provided polyhydroxyalkanoate comprising at least one unit represented by the following chemical formula in a molecule: —(O—CH(—(CH2)m—CH2-p-C6H4—CONH-A1SO2R1)CH2CO)— (wherein R1 represents OH, a halogen atom, ONa, OK or OR1a; A1 and R1a each represents a substituted or unsubstituted aliphatic hydrocarbon structure, a substituted or unsubstituted aromatic cyclic structure or a substituted or unsubstituted heterocyclic structure; m represents an integer selected from a range of 0 to 7; and R1, A1, R1a and m have the aforementioned meanings independently for each unit when plural units are present), a charge control agent containing such polyhydroxyalkanoate, a toner utilizing such charge control agent, and an image forming method and an image forming apparatus utilizing such toner.

摘要翻译： 本发明提供一种适用于电荷控制剂用电荷控制剂的新型聚酯，其具有电荷稳定性，高电荷量，改进的分散性和生物降解性，以及其制备方法和应用技术。 根据本发明，提供了在分子中包含由以下化学式表示的至少一个单元的聚羟基链烷酸酯：<？in-line-formula description =“In-line Formulas”end =“lead”→> - （O- CH（ - （CH 2 CH 2）m CH 2 CH 2 CH 2 CH 2 CH 2 - -CONH-A 1 2 1 CO 2） （其中R 1表示OH，卤素原子，ONa，OK或OR 1a; A < 取代或未取代的脂族烃结构，取代或未取代的芳族环状结构或取代或未取代的杂环结构; m表示选自以下的整数; R 1，R 2， 0至7;当存在多个单元时，R 1，A 1，R 1a和m对于每个单元具有上述含义 ），含有这种聚羟基链烷酸酯的电荷控制剂 e，使用这种电荷控制剂的调色剂，以及使用这种调色剂的图像形成方法和图像形成装置。

公开(公告)号：US20060211100A1

公开(公告)日：2006-09-21

申请号：US10531689

申请日：2003-10-23

申请人： Takashi Kenmoku ,  Tetsuya Yano ,  Chieko Mihara ,  Shinya Kozaki ,  Tsutomu Honma ,  Tatsuki Fukui ,  Takeshi Imamura ,  Etsuko Sugawa

发明人： Takashi Kenmoku ,  Tetsuya Yano ,  Chieko Mihara ,  Shinya Kozaki ,  Tsutomu Honma ,  Tatsuki Fukui ,  Takeshi Imamura ,  Etsuko Sugawa

IPC分类号： C12P7/62 ,  C08G63/87

CPC分类号： C08G63/06 ,  C08G63/6882 ,  C12P7/625

摘要： The invention provides a PHA copolymer including at least a 3-hydroxy-ω-carboxyalkanoic acid represented by a formula (19) or (32) and simultaneously at least a unit represented by a formula (2) or a formula (3) in a molecule, a precursor PHA copolymer having a corresponding vinyl group or a corresponding alkoxycarbonyl group, a biosynthesis method thereof by microorganisms, and a method of producing a desired PHA copolymer from the precursor PHA copolymer: (wherein k, m, n are integers; R18 represents H, Na, K, R27? represents (A); R1 represents a substituent on a cyclohexyl group and represents H, CN, NO2, a halogen atom, CH3, C2H5, C3H7, CF3, C2F5, or C3F7; R includes a residue including a phenyl structure or a thienyl structure; these being independent for each unit).

摘要翻译： 本发明提供一种PHA共聚物，其包含至少一种由式（19）或（32）表示的3-羟基-ω-羧基烷酸，同时至少由式（2）或式（3）表示的单元在 分子，具有相应的乙烯基或相应的烷氧基羰基的前体PHA共聚物，微生物的生物合成方法以及由前体PHA共聚物制备所需PHA共聚物的方法（其中k，m，n为整数; R 表示H，Na，K，R 27？表示（A）; R 1表示环己基上的取代基，代表H，CN ，NO 2 2，卤素原子，CH 3，C 2 H 5，C 3， H 3，CF 3，C 2 H 5，或C 3 H 3， R> R包括残基 e包括苯基结构或噻吩基结构; 这些对于每个单元是独立的）。

公开(公告)号：US20060172394A1

公开(公告)日：2006-08-03

申请号：US11340728

申请日：2006-01-27

申请人： Shinya Kozaki ,  Tsutomu Honma ,  Tetsuya Yano

发明人： Shinya Kozaki ,  Tsutomu Honma ,  Tetsuya Yano

IPC分类号： C12P17/00 ,  C12P11/00 ,  C12P7/62 ,  C12N1/21

CPC分类号： C12P7/625 ,  C12N1/20 ,  C12P17/00 ,  C12R1/38

摘要： Microorganisms showing higher productivity of unusual-polyhydroxyalkanoate (PHA) than ever by using an inexpensive substrate or under specific conditions are obtained from wild strains and mutant strains. The microorganisms are cultured with such substrates to synthesize an unusual-polyhydroxyalkanoate. The unusual-polyhydroxyalkanoate can be produced with high efficiency at low cost.

摘要翻译： 通过使用廉价的底物或在特定条件下显示异常聚羟基链烷酸酯（PHA）的较高生产力的微生物从野生菌株和突变菌株获得。 用这种底物培养微生物以合成异常聚羟基链烷酸酯。 异常的聚羟基链烷酸酯可以以低成本高效率地生产。